Article
Biotechnology & Applied Microbiology
I. Ziegler, P. Vollmar, M. Knuepfer, P. Braun, K. Stoecker
Summary: The study aimed to determine and compare the limits of detection (LOD) of several commercially available LFAs using viable risk group-3 strains. The results showed significant variations in LODs between different tests and organisms, highlighting the importance of using accurately quantified viable strains for determining LODs.
JOURNAL OF APPLIED MICROBIOLOGY
(2021)
Article
Medicine, General & Internal
Brooke L. Deatherage Kaiser, Dawn N. Birdsell, Janine R. Hutchison, Johanna Thelaus, Sarah C. Jenson, Voahangy Andrianaivoarimanana, Mona Bystroem, Kerstin Myrtennaes, Ryelan F. McDonough, Roxanne D. Nottingham, Jason W. Sahl, Herbert P. Schweizer, Minoarisoa Rajerison, Mats Forsman, David S. Wunschel, David M. Wagner
Summary: Antimicrobial resistance is a growing concern, and we studied the proteins associated with Yersinia pestis and Francisella tularensis strains to identify potential protein signatures for antimicrobial resistance (AMR). We found that protein expression was significantly influenced by AMR, even in the absence of antibiotics in growth media. Additionally, the abundance of specific proteins involved in metabolic pathways and biological functions was altered in AMR strains, regardless of species or resistance mechanism. These features could be used to develop novel approaches for identifying AMR phenotypes.
FRONTIERS IN MEDICINE
(2022)
Article
Microbiology
Shahar Rotem, Ohad Shifman, Moshe Aftalion, David Gur, Tamar Aminov, Ronit Aloni-Grinstein
Summary: The rapid antibiotic susceptibility test method MAPt, which can be performed directly on whole blood samples, offers advantages by omitting time-consuming steps, determining minimal inhibitory concentration quickly, and improving rapid diagnosis and treatment of pathogens.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Ohad Shifman, Tamar Aminov, Moshe Aftalion, David Gur, Hila Cohen, Elad Bar-David, Ofer Cohen, Emanuelle Mamroud, Haim Levy, Ronit Aloni-Grinstein, Ida Steinberger-Levy, Shahar Rotem
Summary: The EUCAST RAST shows promising applicability for rapid antibiotic susceptibility testing of dangerous pathogens in positive blood cultures. This could lead to shorter diagnostic times and prompt antibiotic treatment.
Article
Immunology
Guillain Mikaty, Christian E. Demeure, Sofia Filali, Javier Pizarro-Cerda, Pierre Goossens, Elisabeth Carniel
Summary: This study aimed to determine whether DIC occurs at the late stages of plague and anthrax. The results showed that animals infected with anthrax exhibited typical DIC characteristics, while those infected with plague did not. Therefore, understanding the mechanisms behind these hemorrhages is crucial.
MICROBES AND INFECTION
(2023)
Editorial Material
Biochemistry & Molecular Biology
Verena J. Schuenemann
Summary: Ancient DNA methodologies have the potential to study the past prevalence and evolutionary history of pathogens. A recent study identified bacteria that cause plague and typhoid fever in Minoan Crete, illustrating both the possibilities and limitations of ancient pathogen genomics.
Article
Biotechnology & Applied Microbiology
Joerg Linde, Hanka Brangsch, Martin Hoelzer, Christine Thomas, Mandy C. Elschner, Falk Melzer, Herbert Tomaso
Summary: This study evaluated the application of Oxford Nanopore Technology (ONT) long-read sequencing in highly pathogenic bacteria and found that combining data from ONT and Illumina can be used for high-resolution genotyping, but further improvement is needed for certain bacteria.
Article
Biotechnology & Applied Microbiology
Sergei S. Biryukov, Nathaniel O. Rill, Christopher P. Klimko, Jennifer L. Dankmeyer, Jennifer L. Shoe, Melissa Hunter, Yuli Talyansky, Derrick Hau, Marcellene A. Gates-Hollingsworth, Sujata G. Pandit, David P. Fetterer, Ju Qiu, Michael L. Davies, David P. AuCoin, Christopher K. Cote
Summary: In this study, two screening assays were optimized to evaluate the ability of antibodies to promote phagocytosis of Y. pestis by macrophages and induce a cytokine signature predictive of protection in vivo. The results showed that certain antibodies increased bacterial uptake and produced unique cytokine signatures associated with in vivo protection. These findings are crucial for selecting effective antibodies for plague treatment.
HUMAN VACCINES & IMMUNOTHERAPEUTICS
(2023)
Article
Microbiology
Irina Buchovec, Laurita Klimkaite, Edita Suziedeliene, Saulius Bagdonas
Summary: Antimicrobial photodynamic therapy (aPDT) based on riboflavin and chlorophyllin can effectively inactivate Acinetobacter baumannii and Stenotrophomonas maltophilia, especially for biofilm bacteria.
Review
Biochemistry & Molecular Biology
Wieslaw Swietnicki
Summary: The review discusses strategies for designing vaccines against selected pathogens using bacterial secretory systems and connects them to the biology of the system. Examples are given for Y. pestis, S. enterica, B. anthracis, S. flexneri, and other human pathogens, and their effectiveness and long-term protection are discussed.
Article
Microbiology
Veronika Merold, Kim Silberreis, Kilian Stoecker
Summary: Real-time PCR has become an essential tool in clinical microbiology and key performance indicators of rtPCR assays include specificity, analytical and clinical sensitivity. This study demonstrated the use of naturally pre-amplified ribosomal RNA as a target for highly sensitive rtPCR detection of Yersinia pestis and Francisella tularensis, showing superior performance compared to previous assays. The assays can be applied on heat-inactivated samples without the need of time-consuming nucleic acid extraction, which may have potential for rapid pathogen diagnostics.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
History
Sabine R. Huebner, Brandon T. Mcdonald
Summary: Ancient Egypt played a crucial role in the history of infectious diseases by connecting different civilizations and ecologies, allowing the spread of local and Mediterranean-wide epidemics. However, the understanding of the origins and scope of later outbreaks was muddled due to the influence of Thucydides' account of the Plague of Athens. Critical reading of ancient literature and documents, along with archaeological and paleoscientific evidence, significantly improves our understanding of how Egypt facilitated the circulation of pathogens between the western Indian Ocean and the Mediterranean.
JOURNAL OF INTERDISCIPLINARY HISTORY
(2023)
Review
Biotechnology & Applied Microbiology
Abhinandan Patnaik, Sharad Kumar Rai, Ram Kumar Dhaked
Summary: Bacterial pathogens have always been present in our ecosystem, with some causing deadly outbreaks and being exploited as threats. There are natural hotspots for these pathogens worldwide, making them clinically important. Technological advancements and lifestyle changes have led to the evolution of more virulent and resistant strains. The development of multidrug-resistant bacteria as potential bioweapons is a growing concern. This review highlights encouraging developments in protecting against select biothreat bacterial pathogens.
INDIAN JOURNAL OF MICROBIOLOGY
(2023)
Article
Microbiology
Veronica Elizabeth Alvarez, Maria Paula Quiroga, Daniela Centron
Summary: Since the emergence of high-risk clones, there have been efforts to understand their molecular basis for prevalence in nosocomial settings. A machine learning algorithm has identified a biomarker (U1 sequence) for early and accurate detection of Acinetobacter baumannii global clone 1 (GC1). These findings suggest the potential use of machine learning to identify relevant biomarkers for other multidrug-resistant high-risk clones.
Article
Multidisciplinary Sciences
Amy L. Bogue, Warunya Panmanee, Cameron T. McDaniel, Joel E. Mortensen, Edwin Kamau, Luis A. Actis, Jay A. Johannigman, Michael J. Schurr, Latha Satish, Nalinikanth Kotagiri, Daniel J. Hassett
Summary: The novel biocide AB569, composed of acidified nitrite and ethylenediaminetetraacetic acid, demonstrated bactericidal activity against multi-drug resistant Acinetobacter baumannii and Acinetobacter spp. AB569 treatment can prevent the bacteria from gaining an early growth advantage during infection and lead to extensive bactericidal activity upon subsequent exposures.
Article
Public, Environmental & Occupational Health
Steev Loyola, Luz R. Gutierrez, Gertrudis Horna, Kyle Petersen, Juan Agapito, Jorge Osada, Paul Rios, Andres G. Lescano, Jesus Tamariz
AMERICAN JOURNAL OF INFECTION CONTROL
(2016)
Article
Physiology
Yan Bai, Nandakumar Selvaraj, Kyle Petersen, Richard Mahon, William A. Cronin, Joseph White, Peter R. Brink, Ki H. Chon
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY
(2013)
Article
Public, Environmental & Occupational Health
Seth Y. Flagg, David P. Regis, Kyle Petersen, Richard T. Mahon
AVIATION SPACE AND ENVIRONMENTAL MEDICINE
(2013)
Article
Public, Environmental & Occupational Health
G. J. Da Silva, T. Van der Reijden, S. Domingues, N. Mendonca, K. Petersen, L. Dijkshoorn
EPIDEMIOLOGY AND INFECTION
(2014)
Review
Infectious Diseases
Kyle Petersen, Paige Waterman
EXPERT REVIEW OF ANTI-INFECTIVE THERAPY
(2011)
Article
Microbiology
Kyle Petersen, Suzanne C. Cannegieter, Tanny J. van der Reijden, Beppie Van Strijen, David M. You, Britta S. Babel, Andrew I. Philip, Lenie Dijkshoorn
JOURNAL OF CLINICAL MICROBIOLOGY
(2011)
Review
Critical Care Medicine
Duane R. Hospenthal, Clinton K. Murray, Romney C. Andersen, R. Bryan Bell, Jason H. Calhoun, Leopoldo C. Cancio, John M. Cho, Kevin K. Chung, Jon C. Clasper, Marcus H. Colyer, Nicholas G. Conger, George P. Costanzo, Helen K. Crouch, Thomas K. Curry, Laurie C. D'Avignon, Warren C. Dorlac, James R. Dunne, Brian J. Eastridge, James R. Ficke, Mark E. Fleming, Michael A. Forgione, Andrew D. Green, Robert G. Hale, David K. Hayes, John B. Holcomb, Joseph R. Hsu, Kent E. Kester, Gregory J. Martin, Leon E. Moores, William T. Obremskey, Kyle Petersen, Evan M. Renz, Jeffrey R. Saffle, Joseph S. Solomkin, Deena E. Sutter, David R. Tribble, Joseph C. Wenke, Timothy J. Whitman, Andrew R. Wiesen, Glenn W. Wortmann
JOURNAL OF TRAUMA-INJURY INFECTION AND CRITICAL CARE
(2011)
Review
Critical Care Medicine
Kyle Petersen, Marcus H. Colyer, David K. Hayes, Robert G. Hale, R. Bryan Bell
JOURNAL OF TRAUMA-INJURY INFECTION AND CRITICAL CARE
(2011)
Review
Critical Care Medicine
Gregory J. Martin, James R. Dunne, John M. Cho, Joseph S. Solomkin
JOURNAL OF TRAUMA-INJURY INFECTION AND CRITICAL CARE
(2011)
Review
Critical Care Medicine
Duane R. Hospenthal, Andrew D. Green, Helen K. Crouch, Judith F. English, Jane Pool, Heather C. Yun, Clinton K. Murray
JOURNAL OF TRAUMA-INJURY INFECTION AND CRITICAL CARE
(2011)
Review
Critical Care Medicine
Laurie C. D'Avignon, Kevin K. Chung, Jeffery R. Saffle, Evan M. Renz, Leopoldo C. Cancio
JOURNAL OF TRAUMA-INJURY INFECTION AND CRITICAL CARE
(2011)
Review
Critical Care Medicine
Michael A. Forgione, Leon E. Moores, Glenn W. Wortmann
JOURNAL OF TRAUMA-INJURY INFECTION AND CRITICAL CARE
(2011)
Review
Critical Care Medicine
Clinton K. Murray, William T. Obremskey, Joseph R. Hsu, Romney C. Andersen, Jason H. Calhoun, Jon C. Clasper, Timothy J. Whitman, Thomas K. Curry, Mark E. Fleming, Joseph C. Wenke, James R. Ficke
JOURNAL OF TRAUMA-INJURY INFECTION AND CRITICAL CARE
(2011)
Article
Medicine, General & Internal
Kyle Petersen, Ting Dong, Paul A. Hemmer, William F. Kelly
Summary: This study compared the academic outcomes of virtual patient cases (VPCs) and live lectures, and found no significant difference between the two methods. Therefore, VPCs can be used as a substitute for traditional live lectures in situations where time or resources are limited.