Article
Biochemistry & Molecular Biology
Viktoria Rungelrath, Frank R. DeLeo
Summary: Staphylococcus aureus is a major cause of bacterial infections worldwide, with resistance to antibiotics and the ability to evade host defenses. Research has identified certain virulence molecules of S. aureus as therapeutic targets. Treatment options for methicillin-resistant S. aureus are limited, highlighting the need for further research to develop new therapies.
ANTIOXIDANTS & REDOX SIGNALING
(2021)
Review
Biochemistry & Molecular Biology
Beata Mlynarczyk-Bonikowska, Cezary Kowalewski, Aneta Krolak-Ulinska, Wojciech Marusza
Summary: This paper discusses the mechanisms of drug resistance in S. aureus, including resistance to beta-lactam antibiotics, glycopeptides, oxazolidinones, MLS-B, aminoglycosides and spectinomicin, fluoroquinolones, tetracyclines, mupirocin, fusidic acid, daptomycin, as well as other antibiotics and chemotherapeutics. It also covers the molecular epidemiology of MRSA.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Sarah E. Sansom, Emily Benedict, Stephanie N. Thiede, Bala Hota, Alla Aroutcheva, Darjai Payne, Chad Zawitz, Evan S. Snitkin, Stefan J. Green, Robert A. Weinstein, Kyle J. Popovich
Summary: This study utilized whole-genome sequencing to distinguish MRSA isolates, showing that a combination of clindamycin or levofloxacin susceptibility and resistance to <= 2 antibiotic classes can predict USA300 and USA100 strains. Additionally, resistance to <= 2 classes was found to be predictive for USA300 strains.
MICROBIOLOGY SPECTRUM
(2021)
Article
Multidisciplinary Sciences
Tatiana Dimitriu, Andrew C. Matthews, Angus Buckling
Summary: Conjugative plasmids can rapidly evolve increased transfer rates and antimicrobial resistance when confronted with susceptible hosts. This evolution is driven by mutations in the copA gene, leading to higher copy numbers and subsequently higher transfer rates and AMR. Correlated selection between plasmid transfer and AMR may increase the spread of AMR within populations and communities.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Infectious Diseases
Feiteng Zhu, Hemu Zhuang, Lingfang Di, Zhengan Wang, Yiyi Chen, Shengnan Jiang, Chao Gu, Lu Sun, Haiping Wang, Yiwei Zhu, Peng Lan, Dandan Wu, Yunsong Yu, Shujuan Ji, Yan Chen
Summary: This study evaluated the susceptibility of 472 methicillin-resistant Staphylococcus aureus (MRSA) isolates to ceftobiprole (BPR) and investigated the mechanisms underlying BPR resistance. The results showed that community-associated MRSA isolates were more susceptible to BPR than healthcare-associated MRSA isolates. Nanopore sequencing revealed SCCmec repeat-mediated resistance mechanism in BPR-resistant MRSA isolates, and mutations in multiple genes were found to potentially contribute to BPR-resistance development in MRSA.
CLINICAL MICROBIOLOGY AND INFECTION
(2022)
Article
Agriculture, Dairy & Animal Science
Pengfei Zhang, Xinyu Liu, Jie Zhang, Xueting Fu, Yangli Wan, Hu Pan, Congming Wu, Xin Wang
Summary: The study found that yak butter from Tibet, China, is contaminated with Staphylococcus aureus, including methicillin-resistant strains. The isolates carried various virulence genes, with blaZ being the most frequently detected resistance gene.
JOURNAL OF DAIRY SCIENCE
(2021)
Review
Biochemistry & Molecular Biology
Josefien W. Hommes, Bas G. J. Surewaard
Summary: MRSA infections pose a global health threat and have a high mortality rate. In addition to being an extracellular pathogen, MRSA can also survive and replicate intracellularly, using the antimicrobial environment of phagocytes to evade immune cells and antibiotics. The intracellular niche of bacteria may contribute to the development of antibiotic tolerance, and there are various new antibacterial strategies targeting this intracellular bacterial niche.
Article
Biochemistry & Molecular Biology
Marta Woroszylo, Daria Ciecholewska-Jusko, Adam Junka, Radoslaw Drozd, Marcin Wardach, Pawel Migdal, Patrycja Szymczyk-Ziolkowska, Daniel Styburski, Karol Fijalkowski
Summary: The combination of rotating magnetic field (RMF) with beta-lactam antibiotics shows promising results in enhancing the antibacterial effects against methicillin-resistant Staphylococcus aureus (MRSA) by causing morphological alterations in bacterial cells. This study provides potential new treatment options for MRSA infections by utilizing the disruptive effect of RMF on bacterial cell walls.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Muhammad Mubashar Idrees, Khadija Saeed, Muhammad Akbar Shahid, Muhammad Akhtar, Khadija Qammar, Javariya Hassan, Tayyaba Khaliq, Ali Saeed
Summary: This cross-sectional study aimed to determine the prevalence of mecA and mecC genes among phenotypically identified MRSA and evaluate their effectiveness against different antibiotics. The results showed a high prevalence of mecA and mecC in MRSA samples, and penicillin and amoxicillin/clavulanic acid were ineffective against MRSA, while vancomycin, teicoplanin, and linezolid were the most effective drugs.
Article
Microbiology
Rachida Namoune, Abla Djebbar, Rebecca Mekler, Martin Mchugh, Mohammed El Amine Bekara, Arun Decano, Matthew T. G. Holden, Mohammed Sebaihia
Summary: This study used whole genome sequencing to analyze the genotypes and genetic determinants of antimicrobial resistance in clinical isolates of Staphylococcus aureus from two hospitals in Algeria. The isolates showed high rates of multidrug resistance, with the ST80 type being predominant. The results emphasize the importance of continuous surveillance to monitor the spread of S. aureus in healthcare settings.
Article
Microbiology
Jacqueline L. Gill, Jessica Hedge, Daniel J. Wilson, R. Craig MacLean
Summary: Our research shows that the MRSA strain Staphylococcus aureus ST239 originated from a large-scale recombination between ST8 and ST30 before 1945. Despite being primarily influenced by purifying selection, ST239 has undergone parallel evolution in genes related to antibiotic resistance and virulence. However, ST239 exhibits lower competitive fitness compared to other strains of S. aureus, which may explain its rapid decline in prevalence worldwide.
Article
Microbiology
Pedro Dorado-Morales, M. Pilar Garcillan-Barcia, Inigo Lasa, Cristina Solano
Summary: Plasmids play a major role in the spread of antibiotic resistance genes among bacterial strains. This study demonstrates that the initial fitness cost of plasmids in Staphylococcus aureus can be alleviated over time through plasmid rearrangements mediated by insertion sequences, leading to the loss of antimicrobial resistance genes. The results suggest the potential benefits of reducing antibiotic use in both animal and clinical settings to prevent the spread of clinical multidrug resistance plasmids.
Article
Environmental Sciences
Anne Mette Madsen, Fei Zhang, Yonghui Zeng, Margit W. Frederiksen
Summary: This study investigated the potential human exposure to airborne microorganisms, including MRSA, in a pigeon exhibition in Denmark. The results showed high concentrations of fungi, bacteria, and endotoxin in the air, with concentrations up to 200 times higher than outdoor references. The bacteria found in the exhibition were mainly species found in pigeon coops, indicating that pigeons are the sources of exposure. The presence of airborne MRSA highlights the importance of considering this environment as a potential place of exchange of resistant bacteria between animals and humans.
ENVIRONMENTAL RESEARCH
(2023)
Review
Infectious Diseases
Martyna Kasela, Mateusz Ossowski, Ewelina Dzikon, Katarzyna Ignatiuk, Lukasz Wlazlo, Anna Malm
Summary: Methicillin-resistant Staphylococcus aureus (MRSA) is a significant cause of infectious diseases in humans and animals, resulting in economic losses in human healthcare and the farming sector. The changing epidemiology of MRSA globally affects animal welfare and public health concerns. Livestock have high rates of MRSA colonization, raising questions about reservoirs and transmission, while companion animals vary in MRSA colonization and infection rates, impacting human health. This article highlights the circulation of animal-associated MRSA (AA-MRSA) in the environment and factors influencing its transmission, as well as successful strategies to limit its spread among animals and humans.
Article
Multidisciplinary Sciences
Zhengan Wang, Chao Gu, Lu Sun, Feng Zhao, Ying Fu, Lingfang Di, Junxiong Zhang, Hemu Zhuang, Shengnan Jiang, Haiping Wang, Feiteng Zhu, Yiyi Chen, Mengzhen Chen, Xia Ling, Yan Chen, Yunsong Yu
Summary: Staphylococcus capitis is a common cause of bloodstream infections in neonatal intensive care units, with multidrug-resistant isolates complicating treatment. The aim of this study was to establish a core genome multilocus sequence typing (cgMLST) scheme to document the transmission and dissemination of multidrug-resistant S. capitis isolates.
NATURE COMMUNICATIONS
(2022)
Article
Infectious Diseases
K. Johani, M. Malone, S. O. Jensen, H. G. Dickson, I. B. Gosbell, H. Hu, Q. Yang, G. Schultz, K. Vickery
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2018)
Article
Infectious Diseases
K. Johani, B. G. Fritz, T. Bjarnsholt, B. A. Lipsky, S. O. Jensen, M. Yang, A. Dean, H. Hu, K. Vickery, M. Malone
CLINICAL MICROBIOLOGY AND INFECTION
(2019)
Article
Infectious Diseases
Vidthiya Menon, Rebecca Davis, Nick Shackel, Bjorn A. Espedido, Alicia G. Beukers, Slade O. Jensen, Sebastiaan J. van Hal
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2018)
Article
Public, Environmental & Occupational Health
Andie S. Lee, Elizabeth White, Leigh G. Monahan, Slade O. Jensen, Raymond Chan, Sebastiaan J. van Hal
INFECTION CONTROL AND HOSPITAL EPIDEMIOLOGY
(2018)
Article
Public, Environmental & Occupational Health
A. Almatroudi, S. Tahir, H. Hu, D. Chowdhury, I. B. Gosbell, S. O. Jensen, G. S. Whiteley, A. K. Deva, T. Glasbey, K. Vickery
JOURNAL OF HOSPITAL INFECTION
(2018)
Review
Microbiology
Sally R. Partridge, Stephen M. Kwong, Neville Firth, Slade O. Jensen
CLINICAL MICROBIOLOGY REVIEWS
(2018)
Article
Public, Environmental & Occupational Health
C. E. McCafferty, D. Abi-Hanna, M. J. Aghajani, G. T. Micali, I Lockart, K. Vickery, I. B. Gosbell, S. O. Jensen
JOURNAL OF HOSPITAL INFECTION
(2018)
Article
Public, Environmental & Occupational Health
D. Chowdhury, A. Rahman, H. Hu, S. O. Jensen, A. K. Deva, K. Vickery
JOURNAL OF HOSPITAL INFECTION
(2019)
Article
Microbiology
Sarah L. Baines, Slade O. Jensen, Neville Firth, Anders Goncalves da Silva, Torsten Seemann, Glen P. Carter, Deborah A. Williamson, Benjamin P. Howden, Timothy P. Stinear
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2019)
Article
Biochemistry & Molecular Biology
Saskia Schwarzer, Michael Radzieta, Slade O. Jensen, Matthew Malone
Summary: A topical desiccating wound agent containing specific ingredients was evaluated for its efficacy against biofilms produced by two bacterial strains, demonstrating complete eradication in different in vitro models.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Elena Gomez-Sanz, Jose Manuel Haro-Moreno, Slade O. Jensen, Juan J. Roda-Garcia, Mario Lopez-Perez
Summary: This study identified a novel transferable staphylococcal TMP resistance gene, dfrE, in MRSS strain C2865, shedding light on the genome plasticity of S. sciuri and its role as a resourceful reservoir for adaptive traits. The data highlight the importance of genome-wide ecological studies in understanding bacterial adaptive features.
Article
Microbiology
Ana Domazetovska, Slade O. Jensen, Matthew Gray, Michael Radzieta, Michael Maley
Summary: This study developed a CRISPR/Cas9-based targeted next-generation sequencing (NGS) method for direct sequencing of Legionella pneumophila from patient samples. The method was more efficient than whole-genome sequencing and accurately classified patients. This method has the potential to be widely applicable in microbial outbreak investigations.
MICROBIOLOGY SPECTRUM
(2022)
Review
Biochemistry & Molecular Biology
Xian Zhou, Paayal Kumar, Deep J. Bhuyan, Slade O. Jensen, Tara L. Roberts, Gerald W. Muench
Summary: This narrative review proposes that neuroinflammation in Alzheimer's disease (AD) may be partially caused by external pathogens entering the brain through the nose and the olfactory system. The olfactory system represents a plausible route for pathogen entry, and accidental exposure of the olfactory mucosa to hands contaminated with soil and feces when picking one's nose is one potential mechanism.
Article
Microbiology
Neville Firth, Slade O. Jensen, Stephen M. Kwong, Ronald A. Skurray, Joshua P. Ramsay
MICROBIOLOGY SPECTRUM
(2018)
Letter
Immunology
Rowena McMullan, Vidthiya Menon, Alicia G. Beukers, Slade O. Jensen, Sebastiaan J. van Hal, Rebecca Davis
EMERGING INFECTIOUS DISEASES
(2018)