Assessing directed evolution methods for the generation of biosynthetic enzymes with potential in drug biosynthesis

Laboratory Evolution of Stereoselective Enzymes: A Prolific Source of Catalysts for Asymmetric Reactions

(2010) Manfred T. Reetz   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

High-Throughput Metabolic Engineering: Advances in Small-Molecule Screening and Selection

(2010) Jeffrey A. Dietrich et al.   Annual Review of Biochemistry

Iterative Saturation Mutagenesis Accelerates Laboratory Evolution of Enzyme Stereoselectivity: Rigorous Comparison with Traditional Methods

(2010) Manfred T. Reetz et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Expanding metabolism for total biosynthesis of the nonnatural amino acid L-homoalanine

(2010) K. Zhang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Design and directed evolution of a dideoxy purine nucleoside phosphorylase

(2010) David P. Nannemann et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Biocatalytic Asymmetric Synthesis of Chiral Amines from Ketones Applied to Sitagliptin Manufacture

(2010) C. K. Savile et al.   SCIENCE

Computational Design of an Enzyme Catalyst for a Stereoselective Bimolecular Diels-Alder Reaction

(2010) J. B. Siegel et al.   SCIENCE

Directed evolution of endoglucanase III (Cel12A) from Trichoderma reesei

(2009) Hikaru Nakazawa et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Directed evolution: new parts and optimized function

(2009) Michael J Dougherty et al.   CURRENT OPINION IN BIOTECHNOLOGY

Biocatalysis in development of green pharmaceutical processes

(2009) Junhua Tao et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Engineering Monolignol 4-O-Methyltransferases to Modulate Lignin Biosynthesis

(2009) Mohammad-Wadud Bhuiya et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Structure-Based and Random Mutagenesis Approaches Increase the Organophosphate-Degrading Activity of a Phosphotriesterase Homologue from Deinococcus radiodurans

(2009) Renda Hawwa et al.   JOURNAL OF MOLECULAR BIOLOGY

Catalytic Turnover-Based Phage Selection for Engineering the Substrate Specificity of Sfp Phosphopantetheinyl Transferase

(2009) Murat Sunbul et al.   JOURNAL OF MOLECULAR BIOLOGY

Design and engineering of an O2 transport protein

(2009) Ronald L. Koder et al.   NATURE

Directed evolution of an orthogonal nucleoside analog kinase via fluorescence-activated cell sorting

(2009) Lingfeng Liu et al.   NUCLEIC ACIDS RESEARCH

Establishing wild-type levels of catalytic activity on natural and artificial (  )8-barrel protein scaffolds

(2009) J. Claren et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Distributions of enzyme residues yielding mutants with improved substrate specificities from two different directed evolution strategies

(2009) J. Paramesvaran et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Two alternative modes for optimizing nylon-6 byproduct hydrolytic activity from a carboxylesterase with a β-lactamase fold: X-ray crystallographic analysis of directly evolved 6-aminohexanoate-dimer hydrolase

(2009) Taku Ohki et al.   PROTEIN SCIENCE

Protein Design by Directed Evolution

(2008) Christian Jäckel et al.   Annual Review of Biophysics

Improved catalytic efficiency of Endo-β-1,4-glucanase from Bacillus subtilis BME-15 by directed evolution

(2008) Ling Lin et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Expanding substrate specificity of GT-B fold glycosyltransferase via domain swapping and high-throughput screening

(2008) Sung-Hee Park et al.   BIOTECHNOLOGY AND BIOENGINEERING

Identification of amino acid residues involved in 4-chloroindole 3-hydroxylation by cytochrome P450 2A6 using screening of random libraries

(2008) Zhi-Gang Zhang et al.   JOURNAL OF BIOTECHNOLOGY

Evolutionary History of a Specialized P450 Propane Monooxygenase

(2008) Rudi Fasan et al.   JOURNAL OF MOLECULAR BIOLOGY

A Study in Molecular Contingency: Glutamine Phosphoribosylpyrophosphate Amidotransferase is a Promiscuous and Evolvable Phosphoribosylanthranilate Isomerase

(2008) Wayne M. Patrick et al.   JOURNAL OF MOLECULAR BIOLOGY

Kemp elimination catalysts by computational enzyme design

(2008) Daniela Röthlisberger et al.   NATURE

Highly L and D enantioselective variants of horseradish peroxidase discovered by an ultrahigh-throughput selection method

(2008) E. Antipov et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

De Novo Computational Design of Retro-Aldol Enzymes

(2008) L. Jiang et al.   SCIENCE