Article
Chemistry, Physical
Peicheng Sun, Zhiyu Huang, Sanchari Banerjee, Marco A. S. Kadowaki, Romy J. Veersma, Silvia Magri, Roelant Hilgers, Sebastian J. Muderspach, Christophe V. F. P. Laurent, Roland Ludwig, David Cannella, Leila Lo Leggio, Willem J. H. van Berkel, Mirjam A. Kabel
Summary: In this study, two new fungal oxidoreductases from the AA16 family were characterized. These enzymes were found to be capable of producing H2O2 by oxidizing low-molecular-weight reductants. The interplay between AA16 proteins and MtLPMO9 proteins significantly enhanced cellulose degradation. These findings provide new insights into the functions of copper-dependent enzymes and the interplay of oxidative enzymes within fungal systems to degrade lignocellulose.
Article
Biotechnology & Applied Microbiology
Jingen Li, Bingchen Chen, Shuying Gu, Zhen Zhao, Qian Liu, Tao Sun, Yongli Zhang, Taju Wu, Defei Liu, Wenliang Sun, Chaoguang Tian
Summary: This study successfully designed and constructed a novel biorefinery system named DMCC, which efficiently converts raw plant biomass and CO2 into organic acids, presenting a promising strategy for cost-effective production of value-added compounds in biorefinery. The DMCC system is one of the great options for realization of carbon neutral economy.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Environmental Sciences
Angela Piersanti, Katre Juganson, Matteo Mozzicafreddo, Wei Wei, Jing Zhang, Kangping Zhao, Patrizia Ballarini, Monika Mortimer, Sandra Pucciarelli, Wei Miao, Cristina Miceli
Summary: The study found that silver nanoparticles (AgNPs) and soluble silver salts have different toxicity mechanisms towards non-target organisms, with AgNPs affecting a greater number of cellular pathways. Additionally, some effects were only triggered by AgNPs, not soluble silver ions.
ENVIRONMENTAL POLLUTION
(2021)
Article
Chemistry, Multidisciplinary
Xing Qin, Jiahuan Zou, Kun Yang, Jinyang Li, Xiaolu Wang, Tao Tu, Jie Zhang, Xiaoyun Su, Bin Yao, Huoqing Huang, Huiying Luo
Summary: Transcriptomic analysis revealed abundant expression of AA9 LPMOs in Myceliophthora thermophila during lignocellulose degradation. Phenotypic characterization of single gene deletion mutants and functional characterization of AA9 LPMOs showed differences in oxidative regioselectivity, catalytic activity, and substrate specificity. Moreover, synergistic actions between AA9 LPMOs and GHs increased the catalytic efficiency of cellulolytic GHs.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2023)
Article
Biotechnology & Applied Microbiology
Monika Tolgo, Silvia Huttner, Peter Rugbjerg, Nguyen Thanh Thuy, Vu Nguyen Thanh, Johan Larsbrink, Lisbeth Olsson
Summary: The study sequenced and analyzed the genome and transcriptome of the thermophilic Ascomycete Thielavia terrestris strain LPH172, revealing a rich arsenal of CAZymes and auxiliary activity (AA) domains. Upregulation of different encoding genes on different substrates suggests a complex and nuanced degradation strategy employed by the fungus.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Agricultural Engineering
Eva Schmitz, Savvina Leontakianakou, Siri Norlander, Eva Nordberg Karlsson, Patrick Adlercreutz
Summary: This study explores the potential of using different enzymes to synergistically degrade agricultural by-products, finding that ferulic acid esterase and xylanases have synergy on all substrates, while different xylanases are particularly effective on oat hulls.
BIORESOURCE TECHNOLOGY
(2022)
Article
Agricultural Engineering
Xing Qin, Jiahuan Zou, Kun Yang, Jinyang Li, Xiaolu Wang, Tao Tu, Yuan Wang, Bin Yao, Huoqing Huang, Huiying Luo
Summary: The thermophilic fungus Myceliophthora thermophila secretes various enzymes for cellulose degradation, including cellulases GH5_5, GH6, GH7, and lytic polysaccharide monooxygenases (LPMOs) AA9. The core cellulolytic enzymes were identified as MtCel7A and MtCel5A, and their hydrolytic efficiencies were improved by C1/C4 oxidizing LPMO9H but inhibited by C1 oxidizing LPMO9E and C4 oxidizing LPMO9J. These findings suggest the potential application of C1/C4 oxidizing LPMOs for efficient conversion of cellulose into biofuels and biochemicals.
BIORESOURCE TECHNOLOGY
(2022)
Article
Microbiology
Zichen Zhang, Aabid Manzoor Shah, Hassan Mohamed, Yao Zhang, Nino Tsiklauri, Yuanda Song
Summary: Cerrena unicolor is a basidiomycete with high lignocellulose degrading ability. The genome sequence of C. unicolor SP02 was reported in this study, revealing its potential application in lignocellulosic biomass conversion. The genetic basis and molecular mechanisms for lignocellulosic degradation were explored, providing insights for industrial biotechnology.
Article
Biotechnology & Applied Microbiology
Yuanhao Ding, Rui Zhang, Longfu Zhu, Maojun Wang, Yizan Ma, Daojun Yuan, Nian Liu, Haiyan Hu, Ling Min, Xianlong Zhang
Summary: This study investigated the yield heterosis of the elite cotton hybrid variety 'Huaza Mian H318' (H318) and its parental lines over a four-year period. Results showed that H318 exhibited higher stability in boll number, faster growth, and increased weight compared to its parental lines. Transcriptome analysis revealed that H318 had enhanced photosynthesis and metabolic capabilities at the seedling stage, contributing to its heterosis.
Article
Energy & Fuels
Anica Dadwal, Shilpa Sharma, Tulasi Satyanarayana
Summary: This study attempts to develop an enzyme cocktail for the conversion of agricultural residues to ethanol. By utilizing recombinant enzymes and optimizing pretreatment methods, high saccharification rate and ethanol production levels were achieved.
BIOENERGY RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Vania Cardoso, Joana L. A. Bras, Ines F. Costa, Luis M. A. Ferreira, Luis T. Gama, Renaud Vincentelli, Bernard Henrissat, Carlos M. G. A. Fontes
Summary: In this study, a high-throughput strategy was used to successfully isolate and clone a diverse library of carbohydrate-active enzyme genes. Most of the genes showed good yield during recombinant expression, with bacteria having the highest soluble protein percentage. Gene synthesis resulted in lower soluble protein production, but the use of a green fluorescent protein tag improved solubility. It was also observed that the amino acid composition of a protein influenced its solubility in Escherichia coli.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Nabieu Kamara, Yamin Jiao, Weicong Huang, Lichong Cao, Lianjun Zhu, Chongchong Zhao, Xu Huang, Fimanekeni Ndaitavela Shivute, Xiangdong Liu, Jinwen Wu, Muhammad Qasim Shahid
Summary: This study aims to investigate the genetic defects related to pollen development and infertility in the novel tetraploid rice germplasm NY2, known as Neo-tetraploid rice. Combined cytological and transcriptome analysis revealed that NY2 gene regulates abnormal tapetal cells, microspore, and middle layer development, leading to pollen abortion and male sterility. Down-regulated transcription factor genes related to pollen development were also identified. This study provides important understanding of the molecular processes underlying fertility control in tetraploid rice.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biotechnology & Applied Microbiology
Wenxiang Lu, Qin Zhou, Yi Chen
Summary: RNA sequencing is an innovative technology for studying transcriptomes, but clinical specimens face challenges due to sample degradation. This study replicated the process of RNA degradation and found that even slight levels of degradation can significant affect RNA expression.
Article
Cell Biology
Di Wu
Summary: The germinal vesicle (GV) stage is a critical transition point in mammalian oocyte development that determines oocyte quality through active RNA degradation and transcriptome reprofiling. Oocyte RNA-seq has revealed differences between non-surrounded nucleolus (NSN) and surrounded nucleolus (SN) phases. Single oocyte RNA-seq (soRNA-seq) shows higher reproducibility and a better illustration of gene changes during oocyte development.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Plant Sciences
Xin Wang, Yue Liu, Zhongkui Han, Yuning Chen, Dongxin Huai, Yanping Kang, Zhihui Wang, Liying Yan, Huifang Jiang, Yong Lei, Boshou Liao
Summary: The study identified key genes and metabolites related to cold tolerance in peanut, with cold-tolerant cultivars showing higher accumulation of certain substances and quicker responses under low temperature stress. Transcriptome sequencing and metabolomic analysis provided valuable insights into the molecular mechanisms of cold tolerance in peanut, laying a foundation for genetic modification to enhance stress tolerance in crops.
FRONTIERS IN PLANT SCIENCE
(2021)