期刊
FUNGAL GENETICS AND BIOLOGY
卷 53, 期 -, 页码 34-41出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.fgb.2013.01.007
关键词
Calcineurin; Nitrogen; Signalling; Calcium; Yeast; Hansenula polymorpha
资金
- Ministerio de Ciencia e Innovacion (MICINN, Spain) [BFU2010-16192]
- Gobierno de Canarias [PI 2008/338]
- Agencia Canaria de Investigacion e Innovacion y Sociedad de la Informacion (ACIISI)
Non-preferred nitrogen sources, unlike preferred ones, raised total cell Ca2+ content and expression of ENA1, a very well-known calcineurin-regulated gene. This indicates calcineurin activation is regulated by nitrogen source. Nitrogen catabolite repression (NCR) and nitrate induction mechanisms, both regulating nitrate assimilation in Hansenula polymorpha, are controlled by calcineurin. Concerning NCR, lack of calcineurin (cnb1 mutant) decreased nitrate-assimilation gene expression, levels of the transcription factor Gat1 and growth in several nitrogen sources. We found that the role of calcineurin in NCR was mediated by Crz1 via Gat1. Regarding nitrate induction, calcineurin also affects the levels of transcription factors Gat2 and Yna2 involved in this process. We conclude that Ca2+ and calcineurin play a central role in nitrogen signalling and assimilation. Thus, the nitrogen source modulates Ca2+ content and calcineurin activation. Calcineurin in turn regulates nitrogen assimilation genes. (C) 2013 Elsevier Inc. All rights reserved.
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