期刊
FUNGAL GENETICS AND BIOLOGY
卷 47, 期 7, 页码 608-618出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.fgb.2010.03.003
关键词
Arbuscular mycorrhiza; Intra-radical mycelium; Two-dimensional electrophoresis; MS/MS; Fungal proteins; Proteomics; Symbiosis; Mycorrhiza-related proteins
资金
- Conseil Regional de Bourgogne [07 9201 CPER 02 S 5524]
In the absence of sequenced genomes for arbuscular mycorrhizal (AM) fungi, their obligatory biotrophy makes their intra-radical biology especially recalcitrant to functional analyses. Because tandem mass spectrometry-based proteomics enables fungal gene product identifications in phyla lacking genomic information, we have compared as a way to enlarge the coverage of in planta expressed-mycorrhiza-related proteins, the root proteome responses of Medicago truncatula upon colonisation with two AM fungi, Glomus mosseae and G. intraradices, using two-dimensional electrophoresis. In contrast to phosphate fertilization, mycorrhization led to specific changes in the abundance of 99 spots, including 42 overlapping modifications between G. mosseae- and G. intraradices-colonised roots. The 32 confident identifications that could be retrieved following tandem mass spectrometry encompassed 21 fungal proteins whose homology-inferred functions were found to complement the working models so far proposed for the intra-radical functioning of AM fungi with regard to carbon utilization, energy generation, redox homeostasis and protein turnover-related processes. (C) 2010 Elsevier Inc. All rights reserved.
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