Article
Chemistry, Multidisciplinary
Joonyoung Lee, Ki-Yoon Kim, Yongchai Kwon, Dahl-Young Khang
Summary: This study proposes stretchable enzymatic biofuel cells (EBFCs) with high performance for power supply. The buckled gold electrodes increase the electrode surface area and catalyst loading, resulting in improved reaction rates. The evaluated EBFCs achieve a maximum power density of 7.1±0.64μW cm-2.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
V. Saska, U. Contaldo, I. Mazurenko, A. de Poulpiquet, E. Lojou
Summary: The nature, composition and concentration of electrolytes play a crucial role in electrocatalysis involving redox enzymes. In this study, the effect of different electrolyte compositions with increasing ionic strengths on the stability and activity of the bilirubin oxidase (BOD) from Myrothecium verrucaria was investigated. It was found that the catalytic current decreased reversibly with increasing salt concentrations on carbon nanotube-modified electrodes, while on gold electrodes modified with self-assembled thiols, the catalytic current decreased reversibly when the enzyme was adsorbed and increased when the enzyme was covalently attached.
BIOELECTROCHEMISTRY
(2023)
Article
Energy & Fuels
Priyanki Das, Vinay Bachu, Lepakshi Barbora, Arup Dutta, Mrinal Kumar Sarma, Pranab Goswami
Summary: This study presents critical findings on a methanol-fueled enzymatic biofuel cell using alcohol oxidase and bilirubin oxidase as catalysts, demonstrating high operational stability through efficient design improvements.
Article
Chemistry, Multidisciplinary
Yan Liu, Zhen Chen, Zhifang Shao, Rong Guo
Summary: Heme nanozymes display catalytic activity similar to peroxidases, and with the help of gold nanoparticles, the heme cofactor can maintain a low-valence state to trigger oxidase-like catalytic function.
CHEMICAL COMMUNICATIONS
(2021)
Article
Biophysics
Tiago Monteiro, Miguel Moreira, Sara B. R. Gaspar, Maria Gabriela Almeida
Summary: This study proposes a new mono-enzymatic oxygen scavenging system using multicopper oxidase as a single biocatalytic oxygen reducer. This system shows quick and effective reduction of oxygen and can be applied in voltammetric biosensors without the need for a second enzyme to handle oxygen reactive intermediaries.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Yanqun Chen, Min Zhou, Jinhua Yang, Yueming Tan, Wenfang Deng, Qingji Xie
Summary: This study presents a new strategy for sensitive PEC immunoassay by modifying the surface charge of photoactive materials. By utilizing CaCO3 nanoparticles as labels to regulate the surface charge of CdS@HMP QDs, sensitive detection of CEA is achieved with a linear detection range of 0.005-50 ng mL(-1) and a detection limit of 1 pg mL(-1).
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Physical
Sabrina Bichon, Priscilla Staigre, Sebastien Gounel, Tatjana Safarik, Cristina Carucci, Alexander Kuhn, Nicolas Mano
Summary: The researchers have developed a strategy to stabilize and maintain enzyme activity in mixed-solvent systems by using engineered enzymes and tuned osmium-based redox polymers. They have validated this approach and demonstrated the wide applicability of the electrode in various organic solvents.
JOURNAL OF CATALYSIS
(2023)
Article
Multidisciplinary Sciences
Hucheng Chang, Neus Gacias Amengual, Alexander Botz, Lorenz Schwaiger, Daniel Kracher, Stefan Scheiblbrandner, Florian Csarman, Roland Ludwig
Summary: This study demonstrates the activity of Lytic polysaccharide monooxygenase (LPMO) on wood cell walls and suggests that even low levels of hydrogen peroxide can support the enzymatic degradation of wood biomass. LPMO plays a synergistic role in fungal extracellular catabolism and industrial biomass degradation.
NATURE COMMUNICATIONS
(2022)
Article
Nanoscience & Nanotechnology
Sahar Cheraghi Shahi, Mehdi Dadmehr, Behnaz Korouzhdehi, Abolfazl Tavassoli
Summary: A colorimetric competitive assay for detecting AFB1 in foods was developed based on the interaction of AuNPs@gelatin. The method showed a linear detection range of 10 to 140 pg ml(-1), with a detection limit of 4 pg ml(-1) and recoveries percentage of 92.4%-95.3% in real saffron samples. This approach offers a simple, cost effective, and specific method for AFB1 toxin detection in food samples.
Article
Engineering, Biomedical
Justyna Sulej, Wiktoria Piatek-Golda, Marcin Graz, Katarzyna Szalapata, Piotr Wasko, Ewa Janik-Zabrotowicz, Monika Osinska-Jaroszuk
Summary: This study aimed to intensify the synthesis of lactobionic acid (LBA) using immobilised enzymes on chitosan microspheres. The results show that LBA immobilised on chitosan microspheres has great potential for medical applications.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
Article
Engineering, Chemical
T. D. Le, D. Lasseux, L. Zhang, C. Carucci, S. Gounel, S. Bichon, F. Lorenzutti, A. Kuhn, T. Safarik, N. Mano
Summary: This work focuses on a multi-scale modeling of coupled diffusion and reaction in a porous micro electrode operating in the Direct Electron Transfer mode. By developing and validating the model, it provides an operational simple way of understanding and further optimizing the functioning of porous electrodes in DET mode.
CHEMICAL ENGINEERING SCIENCE
(2022)
Article
Materials Science, Multidisciplinary
Bing Xu, Xiaoying Xiang, Zhuomei Ding, Zhihui Luo, Jianglou Huang
Summary: A colorimetric assay for glucose detection based on enzymatic etching of triangular gold nanosheets was developed. The presence of glucose oxidase catalyzed the oxidation of glucose, leading to the etching of triangular gold nanosheets and a blue shift in absorption peak. The sensor showed excellent sensing performance in a glucose range of 0.2-12 mM with a limit of detection of 0.1 mM, making it suitable for clinical diagnosis of diabetes.
MATERIALS CHEMISTRY AND PHYSICS
(2023)
Article
Energy & Fuels
Seongjun Kim, Jungyeon Ji, Yongchai Kwon
Summary: A new biocathode consisting of bilirubin oxidase (BOD), 4-aminophthalic acid (4-APA), and buckypaper (BP) electrode is proposed for enzymatic biofuel cells (EBFCs). By using an electrografting method and 4-APA, the BP surface is modified to have negative charges, forming a BP/4-APA structure. The modified surface enhances the reactivities of the cathodic reaction and improves EBFC performance. The membraneless paper-type EBFCs using the BP/4-APA/BOD biocathode exhibit excellent power density and voltage, making them suitable for power sources in implantable devices.
Article
Food Science & Technology
Hasan Nasiri, Hamed Baghban, Reza Teimuri-Mofrad, Ahad Mokhtarzadeh
Summary: Graphitic carbon nitride supported on magnetic chitosan was synthesized and used as a support for cellobiose dehydrogenase immobilization and as a modifier for glassy carbon electrode in the fabrication of an electrochemical lactose sensor. The composite exhibited excellent electrochemical performance and was successfully applied in lactose quantification in dairy products.
INTERNATIONAL DAIRY JOURNAL
(2023)
Article
Chemistry, Analytical
Eimantas Ramonas, Marius Butkevicius, Sergey Shleev, Marius Dagys, Dalius Ratautas
Summary: The study introduces a novel concept of an enzyme-driven pH electrode system composed of two electrodes, which requires no additional counter or reference electrodes and provides high-precision real-time pH monitoring. Additionally, an in-depth mechanistic study was presented to explain the relationship between electrode potential and pH dependence, offering new insights into the operational principles of bilirubin oxidase at different pH levels.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Vilma Ratautaite, Raimonda Boguzaite, Ernestas Brazys, Deivis Plausinaitis, Simonas Ramanavicius, Urte Samukaite-Bubniene, Mikhael Bechelany, Arunas Ramanavicius
Summary: This study utilized molecularly imprinted polypyrrole (MIP-Ppy) and non-imprinted polypyrrole (NIP-Ppy) modified electrodes to evaluate their performance through pulsed amperometric detection. The interaction between SARS-CoV-2 spike glycoproteins and MIP-Ppy was assessed based on the Anson plot calculations.
Article
Chemistry, Analytical
Viktorija Reinikovaite, Sarunas Zukauskas, Rokas Zalneravicius, Vilma Ratautaite, Simonas Ramanavicius, Vytautas Bucinskas, Monika Vilkiene, Arunas Ramanavicius, Urte Samukaite-Bubniene
Summary: The development of microbial fuel cells based on electro-catalytic processes is a new and sustainable approach to energy systems. Microbial fuel cells use microorganisms to transform chemical energy from renewable organic fuels and reduce pollution. However, not all microorganisms can generate sufficient energy. In this study, we evaluated the feasibility of using Rhizobium anhuiense as a catalyst for microbial fuel cells and found that using menadione as a mediator resulted in the best performance with an open circuit potential of 0.385 mV, maximal power density of 5.5 mu W/cm(2), and anode current of 50 mu A/cm(2) at 0.35 mV.
Review
Chemistry, Analytical
Kasparas Kizys, Antanas Zinovicius, Baltramiejus Jakstys, Ingrida Bruzaite, Evaldas Balciunas, Milda Petruleviciene, Arunas Ramanavicius, Inga Morkvenaite-Vilkonciene
Summary: This review discusses the development of microbial biofuel cells and how similar principles apply to bioelectronic device development. Microorganism-based amperometric biosensors, with their low specificity, can be used to design microbial biofuel cells that can consume a wider range of chemical fuels. Charge transfer efficiency is a major challenge in biofuel cell development, which can be improved using nanomaterials and specific redox mediators. Additionally, the biocompatibility of conducting polymers with microorganisms is explored, including ways to modify cell-walls/membranes and improve charge transfer efficiency for biofuel cell design.
Article
Chemistry, Analytical
Roman Viter, Viktoriia Fedorenko, Inga Gabriunaite, Irina Tepliakova, Simonas Ramanavicius, Viktoriia Holubnycha, Arunas Ramanavicius, Ausra Valiuniene
Summary: This study investigates changing/tuning the properties of ZnO by depositing a layer of polydopamine (PDA) on its surface. The electrochemical properties of FTO-ZnO and FTO-ZnO-PDA nanostructures can be changed by varying the applied electrical potential and/or exposure to light. The interaction between ZnO-PDA and bovine serum albumin (BSA) molecules has also been studied, and a possible interaction mechanism has been proposed.
Article
Biophysics
Rokas Zalneravicius, Vidas Pakstas, Giedre Grinciene, Vaclovas Klimas, Algimantas Paskevicius, Kristi Timmo, Marit Kauk-Kuusik, Marius Franckevicius, Gediminas Niaura, Martynas Talaikis, Arunas Jagminas, Arunas Ramanavicius
Summary: In this study, CZTS particles were successfully synthesized using the molten salt approach from raw precursors. SEM analysis revealed the tetragonal shape and crystal size of the CZTS particles. XRD analysis confirmed the presence of a tetragonal crystal structure kesterite phase. The CZTS particles exhibited antimicrobial properties against medically relevant bacteria and yeast strains, with high killing efficiency and the overproduction of reactive oxygen species playing a role in microorganism death.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Article
Biochemistry & Molecular Biology
Viktorija Liustrovaite, Dovydas Karoblis, Benediktas Brasiunas, Anton Popov, Arturas Katelnikovas, Aivaras Kareiva, Arunas Ramanavicius, Roman Viter, Maria Teresa Giardi, Donats Erts, Almira Ramanaviciene
Summary: ZnO nanostructures with different morphologies and particle sizes were evaluated and applied for the development of an immunosensor. The nanostructures included spherical polydisperse structures, compact rod-like spherical structures, and rod-shaped particles. These ZnO nanostructures were mixed with Nafion solution and drop-casted onto screen-printed carbon electrodes, followed by immobilization of prostate-specific antigen (PSA). The affinity interaction of PSA with monoclonal antibodies against PSA was evaluated using the differential pulse voltammetry technique. The detection and quantification limits for anti-PSA were determined for different ZnO nanostructures.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Physics, Applied
Edgaras Markauskas, Laimis Zubauskas, Arnas Naujokaitis, Bronislovas Cechavicius, Martynas Talaikis, Gediminas Niaura, Maria Caplovicova, Viliam Vretenar, Tadas Paulauskas
Summary: Water-assisted ultrashort laser pulse processing is a promising technique for improving the quality of semiconductor materials. This study focuses on femtosecond laser ablation of GaAs in different environments and analyzes the surface morphology, elemental mapping, and spectroscopic properties. The results show that using water as an environment enhances GaAs ablation efficiency and reduces heat-accumulation-related damage, leading to higher material quality in deep trenches.
JOURNAL OF APPLIED PHYSICS
(2023)
Review
Chemistry, Analytical
Greta Pilvenyte, Vilma Ratautaite, Raimonda Boguzaite, Simonas Ramanavicius, Chien-Fu Chen, Roman Viter, Arunas Ramanavicius
Summary: The appearance of biomarkers in body fluids at abnormal concentrations is considered a good tool for detecting disease. New tests that are pathogen-specific, easy to use, and produce results quickly are needed to positively impact healthcare. Molecularly imprinted polymer-based biosensors can achieve these goals and have enormous potential for disease detection.
Article
Electrochemistry
Daiva Bironaite, Jurate Petroniene, Rokas Miksiunas, Antanas Zinovicius, Inga Morkvenaite-Vilkonciene, Arunas Ramanavicius
Summary: This study separated the total population of human skeletal muscle-derived mesenchymal stem/stromal cells (SM-MSCs) into different subpopulations using scanning electrochemical microscopy (AC-SECM) and evaluated their redox changes and myogenic differentiation potential. The results showed that the CD56(+) and CD56(-) subpopulations had different redox activities and myogenic differentiation potential, indicating that the total population of human SM-MSCs is heterogeneous and unevenly responsive to extracellular stimuli.
ELECTROCHIMICA ACTA
(2023)
Article
Biochemistry & Molecular Biology
Ieva Plikusiene, Vincentas Maciulis, Vilius Vertelis, Silvija Juciute, Saulius Balevicius, Arunas Ramanavicius, Julian Talbot, Almira Ramanaviciene
Summary: This study investigated the mechanism of interaction between SCoV2-S protein and specific antibodies using molecular dynamics, revealing that the formation of immune complexes occurs in two stages. By applying a two-step binding model for kinetic analysis, more precise determination of interaction rate constants was achieved compared to the 1:1 Langmuir binding model. These findings provide deeper insights into the formation of antigen-antibody immune complexes and offer a new approach for evaluating antibody affinity towards antigens.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Analytical
Ieva Plikusiene, Vincentas Maciulis, Silvija Juciute, Arunas Ramanavicius, Almira Ramanaviciene
Summary: This study examines the interaction between monoclonal and polyclonal antibodies and the spike protein of SARS-CoV-2 variants. It finds that the B.1.351 variant decreases the dry mass density and increases the wet mass density of the antibody-virus complex. Conversely, the polyclonal antibodies in convalescent human serum lead to a decrease in mass density.
Article
Chemistry, Analytical
Milda Petruleviciene, Irena Savickaja, Jurga Juodkazyte, Arunas Ramanavicius
Summary: This study analyzes the applicability of photoelectrochemical (PEC) sensing of VOCs in aqueous medium using different photoanodes. The results show that aromatic compounds interfere with the PEC sensing of other substances and the WO3 photoanode is suitable for the PEC sensing of methanol, but further optimization is required.
Article
Chemistry, Analytical
Raimonda Boguzaite, Greta Pilvenyte, Vilma Ratautaite, Ernestas Brazys, Almira Ramanaviciene, Arunas Ramanavicius
Summary: This study focuses on the development of a sensor for methylene blue detection using molecularly imprinted polymers. The sensor is designed by molecularly imprinting polypyrrole with methylene blue as a template molecule. Different deposition conditions and the impact of layer durability and thickness were analyzed. The capability of the molecularly imprinted polymer layer to adsorb methylene blue was evaluated using optical absorption changes, and selectivity was monitored using Azure A. Linear relationships were observed in the concentration range of 0.1 μM to 10 mM for methylene blue adsorption.
Article
Optics
Vita Petrikaite, Ilja Ignatjev, Algirdas Selskis, Gediminas Niaura, Evaldas Stankevic
Summary: This study presents the generation of hybrid gold-silver nanoparticles through thermal heating thin metal films on a glass surface with a nanosecond pulsed laser. The optical properties of the obtained nanoparticles can be tuned by varying the ratio and order of the primary films, allowing for control over localized surface plasmon resonance. These engineered substrates act as highly enhanced SERS substrates at different excitation wavelengths and can be produced on a large scale using a laser-based process.
OPTICS AND LASER TECHNOLOGY
(2024)
Article
Chemistry, Multidisciplinary
Elissa Makhoul, Fida Tanos, Maged F. Bekheet, Wiebke Riedel, Eddy Petit, Roman Viter, Iryna Tepliakova, Arunas Ramanavicius, Antonio Razzouk, Geoffroy Lesage, Marc Cretin, Madona Boulos, David Cornu, Mikhael Bechelany
Summary: Some drugs classified as persistent organic pollutants (POPs) cannot be efficiently removed by routine wastewater treatments. A combination of electrocatalysis and a sulfate-based advanced oxidation process via peroxymonosulfate activation has been proven to be an efficient and non-selective method for removing POPs. Perovskites, specifically calcium copper titanate (CCTO), were evaluated as electrode materials due to their unique structural properties and high chemical stability. Through fabrication of porous CCTO anodes with different percentages of polymethyl methacrylate (PMMA), it was found that the CCTO-30 anode exhibited the highest performance in terms of CuO phase amount, defect percentage, oxidation-reduction peak, and resistance. By using CCTO-30 as anodes in a beaker with PMS, paracetamol was completely decomposed due to copper catalytic cycle activation, leading to the generation of radicals and non-radicals that selectively remove paracetamol.
ENVIRONMENTAL SCIENCE-NANO
(2023)
