Article
Biochemistry & Molecular Biology
Jing Lin, Jing Zeng, Sha Liu, Xin Shen, Nan Jiang, Yue-Song Wu, Hong Li, Long Wang, Jianming Wu
Summary: The study demonstrates that 3,3'-di-O-methylellagic acid 4'-glucoside (DMAG) promotes megakaryocyte differentiation and platelet production, showing therapeutic potential for thrombocytopenia.
MOLECULAR MEDICINE
(2021)
Article
Hematology
Huan Dong, Huiyuan Li, Lijun Fang, Anqi Zhang, Xiaofan Liu, Feng Xue, Yunfei Chen, Wei Liu, Ying Chi, Wentian Wang, Ting Sun, Mankai Ju, Xinyue Dai, Renchi Yang, Rongfeng Fu, Lei Zhang
Summary: This study investigated the causes of abnormal platelet function and potential therapeutic strategies in patients with essential thrombocythemia (ET). It was found that platelet aggregation, activation, apoptosis, ROS, and clot retraction were all elevated in ET patients. Inhibiting ROS reduced platelet dysfunction and lowered platelet count.
THROMBOSIS RESEARCH
(2023)
Article
Hematology
Qiuhong Wang, Haocai Chang, Qi Shen, Yonghua Li, Da Xing
Summary: Photobiomodulation therapy (PBMT) prevents chemotherapy-induced thrombocytopenia by increasing platelet production and upregulating hepatic TPO expression. PBMT activates Src protein through reactive oxygen species, leading to the promotion of megakaryocytopoiesis and thrombopoiesis. This research suggests that PBMT is a promising therapeutic strategy for the treatment of CIT.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2021)
Article
Cell Biology
M. Constanza Baroni Pietto, Paola R. Lev, Ana C. Glembotsky, Cecilia P. Marin Oyarzun, Graciela Gomez, Victoria Collado, Cecilia Pisoni, Ramiro A. Gomez, Matias Grodzielski, Jacqueline Gonzalez, Karina Marino, Paula G. Heller, Nora P. Goette, Rosana F. Marta
Summary: The study found that systemic lupus erythematosus (SLE) patients with thrombocytopenia have abnormalities in platelet clearance and production, mainly due to apoptosis and desialylation leading to low platelet count. These abnormalities are more frequently observed in patients with thrombocytopenia and active disease.
Article
Cell Biology
Joanna Nowaczyk, Barbara Poniedzialek, Piotr Rzymski, Dominika Sikora, Mariola Ropacka-Lesiak
Summary: This study aimed to evaluate platelet parameters in pregnant women with fetal growth restriction (FGR) and their relationship with hemodynamic abnormalities. The study found that FGR patients had impaired oxidative metabolism in platelets, which may lead to oxidative stress and impaired platelet function.
Article
Hematology
Weiwei Wang, Shuan Tao, Xia Zhang, Weiguo Wang, Yuanhong Xu, Wei Liang
Summary: The study suggests that bone marrow megakaryocyte count, the proportion of megakaryocytes at each stage, and platelet parameters have important reference value for the clinical diagnosis of primary immune thrombocytopenia (ITP).
CLINICAL AND APPLIED THROMBOSIS-HEMOSTASIS
(2022)
Review
Cell Biology
Cedric Garcia, Baptiste Compagnon, Michael Poette, Marie-Pierre Gratacap, Francois-Xavier Lapebie, Sophie Voisin, Vincent Minville, Bernard Payrastre, Fanny Vardon-Bounes, Agnes Ribes
Summary: Platelets play a crucial role in hemostasis and thrombosis, but they are also involved in mechanisms associated with inflammation, thrombosis, and the immune system in various diseases. During sepsis, platelets enhance the recruitment and activation of immune cells and contribute to pathogen elimination. However, these mechanisms can also lead to severe organ dysfunction.
Review
Medicine, General & Internal
Anurag Singh, Gunalp Uzun, Tamam Bakchoul
Summary: Immune thrombocytopenia (ITP) is an autoimmune disorder characterized by significantly reduced platelet counts in the blood circulation. The complexity of the etiology and heterogeneity of patient characteristics make it challenging for clinicians to choose appropriate therapeutic regimens, emphasizing the importance of considering individual patient factors and preferences in treatment decisions.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Developmental Biology
R. Emily Martin, Eliana Marzol, Jose M. Estevez, Gloria K. Muday
Summary: Research shows that ethylene and its precursor regulate root hair formation by increasing ROS accumulation. Ethylene-sensitive mutants have increased root hair number and ROS accumulation, while ethylene-insensitive mutants show the opposite effect.
Article
Environmental Sciences
Soyeon Jeon, Dong-Keun Lee, Jiyoung Jeong, Sung Ik Yang, Ji-Su Kim, Jinsik Kim, Wan-Seob Cho
Summary: This study investigated the toxicity of microplastics on different cell lines and found that microplastics showed significant toxicity to THP-1 macrophages, with toxicity correlated to the generation potential of reactive oxygen species (ROS). Weathering process reduced the ROS generation potential of microplastics, as weathered microplastics had increased affinity to bind serum protein acting as ROS scavenger.
ENVIRONMENTAL POLLUTION
(2021)
Article
Medicine, Research & Experimental
Daphne N. Pariser, Zachary T. Hilt, Sara K. Ture, Sara K. Blick-Nitko, Mark R. Looney, Simon J. Cleary, Estheany Roman-Pagan, Jerry Saunders, Steve N. Georas, Janelle Veazey, Ferralita Madere, Laura Tesoro Santos, Allison Arne, Nguyen P. T. Huynh, Alison C. Livada, Selena M. Guerrero-Martin, Claire Lyons, Kelly A. Metcalf-Pate, Kathleen E. McGrath, James Palis, Craig N. Morrell
Summary: Platelets, previously known as cellular mediators of thrombosis, are also immune cells that interact with immune cells, impacting their activation and differentiation. Lung megakaryocytes (Mk(L)) have gene expression patterns similar to antigen-presenting cells, and have key immune regulatory roles influenced by the tissue environment. This indicates the plasticity of the immune phenotype of Mk(L) and their ability to induce T cell activation.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Hematology
Manuella Caux, Rana Mansour, Jean-Marie Xuereb, Gaetan Chicanne, Julien Viaud, Alicia Vauclard, Frederic Boal, Bernard Payrastre, Helene Tronchere, Sonia Severin
Summary: This study demonstrates that PIKfyve and its lipid products PtdIns5P and PtdIns(3,5)P-2 play crucial roles in regulating granule integrity in both megakaryocytes and platelets. Inhibition of PIKfyve leads to impaired granule integrity, affecting platelet aggregation and thrombus formation.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2022)
Article
Biology
Christian A. Di Buduo, Pierre-Alexandre Laurent, Carlo Zaninetti, Larissa Lordier, Paolo M. Soprano, Aikaterini Ntai, Serena Barozzi, Alberto La Spada, Ida Biunno, Hana Raslova, James B. Bussel, David L. Kaplan, Carlo L. Balduini, Alessandro Pecci, Alessandra Balduini
Summary: A miniaturized bone marrow tissue model has been developed to predict individual patient responses to Eltrombopag, helping in the selection of personalized treatment for patients with Inherited Thrombocytopenias.
Article
Biochemistry & Molecular Biology
Alina A. Aktanova, Olga S. Boeva, Margarita Sh. Barkovskaya, Ekaterina A. Kovalenko, Ekaterina A. Pashkina
Summary: Reactive oxygen species (ROS) are highly reactive molecules that play important roles in cell signaling and homeostasis. However, at high concentrations, ROS can cause damage to cellular components. Although metal nanoparticles are known to increase ROS production, the effect of cucurbiturils on ROS production is still unknown. In this study, we evaluated the impact of cucurbiturils on ROS production in immune and non-immune cells, as well as tumor cells. Our results showed that cucurbiturils reduced the percentage of ROS-producing cells in platelets, erythrocytes, and activated T-helpers. Further research is needed to better understand the relationship between cucurbiturils and ROS production.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Hematology
Christian Andrea Buduo, Carolina Paula Miguel, Alessandra Balduini
Summary: A State of the Art lecture titled "Megakaryocytes and different thrombopoietic environments" was presented at the ISTH Congress in 2022. It discussed the influence of the bone marrow microenvironment on megakaryocyte maturation, proliferation, and platelet production. The review also highlighted the importance of reproducing the thrombopoietic environment for studying platelet activity and meeting the demand for human platelets. The new data presented during the congress provided insights for the future of megakaryopoiesis.
RESEARCH AND PRACTICE IN THROMBOSIS AND HAEMOSTASIS
(2023)
Article
Biotechnology & Applied Microbiology
Antonella Conforti, Emanuele Marra, Fabio Palombo, Giuseppe Roscilli, Micol Rava, Valeria Fumagalli, Alessia Muzi, Mariano Maffei, Laura Luberto, Lucia Lione, Erika Salvatori, Mirco Compagnone, Eleonora Pinto, Emiliano Pavoni, Federica Bucci, Grazia Vitagliano, Daniela Stoppoloni, Maria Lucrezia Pacello, Manuela Cappelletti, Fabiana Fosca Ferrara, Emanuela D'Acunto, Valerio Chiarini, Roberto Arriga, Abraham Nyska, Pietro Di Lucia, Davide Marotta, Elisa Bono, Leonardo Giustini, Eleonora Sala, Chiara Perucchini, Jemma Paterson, Kathryn Ann Ryan, Amy-Rose Challis, Giulia Matusali, Francesca Colavita, Gianfranco Caselli, Elena Criscuolo, Nicola Clementi, Nicasio Mancini, Ruediger Gross, Alina Seidel, Lukas Wettstein, Jan Muench, Lorena Donnici, Matteo Conti, Raffaele De Francesco, Mirela Kuka, Gennaro Ciliberto, Concetta Castilletti, Maria Rosaria Capobianchi, Giuseppe Ippolito, Luca G. Guidotti, Lucio Rovati, Matteo Iannacone, Luigi Aurisicchio
Summary: This study reports the preclinical evaluation of a novel COVID-19 vaccine candidate based on the electroporation of engineered, synthetic cDNA encoding a viral antigen in the skeletal muscle. The vaccine showed strong immunogenicity and significant protection in animal models, suggesting its potential as an ideal COVID-19 vaccine candidate for clinical development.
Article
Immunology
Valeria Fumagalli, Micol Rava, Davide Marotta, Pietro Di Lucia, Chiara Laura, Eleonora Sala, Marta Grillo, Elisa Bono, Leonardo Giustini, Chiara Perucchini, Marta Mainetti, Alessandro Sessa, Jose M. Garcia-Manteiga, Lorena Donnici, Lara Manganaro, Serena Delbue, Vania Broccoli, Raffaele De Francesco, Patrizia D'Adamo, Mirela Kuka, Luca G. Guidotti, Matteo Iannacone
Summary: In this study, researchers described a method using an inhalation tower system to expose unanesthetized transgenic mice to aerosolized virus. This approach has advantages in simulating COVID-19 pathogenesis and provides a useful tool for studying viral transmission, disease pathogenesis, and therapeutic interventions.
SCIENCE IMMUNOLOGY
(2022)
Article
Cell Biology
Roxana E. Oberkersch, Giovanna Pontarin, Matteo Astone, Marianna Spizzotin, Liaisan Arslanbaeva, Giovanna Pontarin, Emiliano Panieri, Sara Ricciardi, Maria Francesca Allega, Alessia Brossa, Paolo Grumati, Benedetta Bussolati, Stefano Biffo, Saverio Tardito, Massimo M. Santoro
Summary: Cell metabolism plays an important role in angiogenesis, as evidenced by the use of glutaminolysis-derived glutamate to produce aspartate. Aspartate activates the mTORC1 pathway, regulating endothelial translation and pyrimidine synthesis.
DEVELOPMENTAL CELL
(2022)
Editorial Material
Gastroenterology & Hepatology
Luca G. Guidotti, Carlo La Vecchia, Massimo Colombo
Editorial Material
Multidisciplinary Sciences
Matteo Iannacone, Luca G. Guidotti
Summary: According to researchers Matteo Iannacone and Luca G. Guidotti, finding a cure for chronic hepatitis B requires the development of new treatments based on the immune system.
Article
Biochemistry & Molecular Biology
Roberto Aiolfi, Giovanni Sitia, Matteo Iannacone, Ivan Brunetta, Luca G. Guidotti, Zaverio M. Ruggeri
Summary: In viral hemorrhagic fevers, the increase in IFN-I reduces the expression of certain genes involved in lipid biosynthesis and platelet formation, leading to reduced platelet count and defects in the arachidonic acid synthetic pathway. This results in decreased serotonin release from platelets and can cause bleeding when the serotonin level falls below a critical threshold. Transfusion of normal platelets can help control hemorrhage by increasing serotonin release.
Article
Immunology
Valeria Fumagalli, Valentina Venzin, Pietro Di Lucia, Federica Moalli, Xenia Ficht, Gioia Ambrosi, Leonardo Giustini, Francesco Andreata, Marta Grillo, Diletta Magini, Micol Rava, Christin Friedrich, Jason D. Fontenot, Philippe Bousso, Sarah A. Gilmore, Shahzada Khan, Manuel Baca, Eric Vivier, Georg Gasteiger, Mirela Kuka, Luca G. Guidotti, Matteo Iannacone
Summary: Group 1 innate lymphoid cells (ILCs) play a role in controlling T cell-mediated liver immunopathology by limiting local IL-2 concentration.
SCIENCE IMMUNOLOGY
(2022)
Review
Immunology
Matteo Iannacone, Francesco Andreata, Luca G. Guidotti
Summary: HBV can cause acute or chronic liver diseases, with chronic hepatitis often leading to cirrhosis and liver cancer. Immunological events play a crucial role in the outcome of HBV infection, as the virus itself is not highly pathogenic. Current therapies rely on antiviral drugs but cannot completely eradicate the virus from the liver. A cure for chronic hepatitis B may require combining antiviral drugs with immune-stimulating approaches.
CURRENT OPINION IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Alessandra Scagliola, Annarita Miluzio, Giada Mori, Sara Ricciardi, Stefania Oliveto, Nicola Manfrini, Stefano Biffo
Summary: Inhibition of the translation factor eIF6 can limit the progression of nonalcoholic fatty liver disease (NAFLD) to hepatocellular carcinoma (HCC), according to recent research. The study shows that reducing eIF6 levels in mice and using pharmacological inhibitors can reduce the formation and growth of HCC nodules. These findings suggest that targeting eIF6 activity could be a potential therapeutic strategy for managing NAFLD-driven HCC.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Medicinal
Leda Ivanova Bencheva, Lorena Donnici, Luca Ferrante, Adolfo Prandi, Roberta Sinisi, Marilenia De Matteo, Pietro Randazzo, Matteo Conti, Pietro Di Lucia, Elisa Bono, Leonardo Giustini, Maria Vittoria Orsale, Alexandros Patsilinakos, Edith Monteagudo, Matteo Iannacone, Vincenzo Summa, Luca G. Guidotti, Raffaele De Francesco, Romano Di Fabio
Summary: A novel series of SBA-derived CAM with high antiviral activity against HBV replication in CHB patients has been discovered, with compound 10 showing excellent pharmacokinetics in mice and dose-dependent reduction of viral replication. This new SBA CAM offers hope for the development of anti-HBV therapies.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2022)
Article
Biochemistry & Molecular Biology
Nicla Romano, Bruna Di Giacomo, Veronica Nobile, Antonella Borreca, Daniela Willems, Francesca Tilesi, Elisabetta Catalani, Manasi Agrawal, Kristy Welshhans, Sara Ricciardi, Davide Cervia, Marcello Ceci
Summary: This study reveals a mechanism by which FMRP regulates the localization of translational machinery and its translation activity of specific mRNAs by interacting with RACK1. These findings provide new insights into the altered development in Fragile X syndrome.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Ngoc Lan Tran, Lorena Maria Ferreira, Blanca Alvarez-Moya, Valentina Buttiglione, Barbara Ferrini, Paola Zordan, Andrea Monestiroli, Claudio Fagioli, Eugenia Bezzecchi, Giulia Maria Scotti, Antonio Esposito, Riccardo Leone, Chiara Gnasso, Andrea Brendolan, Luca G. Guidotti, Giovanni Sitia
Summary: This study investigated the effects of continuous interferon-alpha (IFN alpha) therapy on the metastasis of colorectal carcinoma (CRC) to the liver using mouse models. The results showed that IFN alpha can control CRC invasion by acting on hepatic endothelial cells (HECs). IFN alpha also strengthens the liver vascular barrier and promotes the generation of antitumor T cells.
Article
Medicine, General & Internal
Annarita Miluzio, Alessandro Cuomo, Chiara Cordiglieri, Lorena Donnici, Elisa Pesce, Mauro Bombaci, Matteo Conti, Alessandra Fasciani, Luigi Terracciano, Lara Manganaro, Mirco Toccafondi, Alessandra Scagliola, Stefania Oliveto, Sara Ricciardi, Renata Grifantini, Raffaele De Francesco, Sergio Abrignani, Nicola Manfrini, Stefano Biffo
Summary: The study found that the binding of the SARS-CoV-2 virus to hACE2 receptors is the first step in infection, but the abundance of receptors is influenced by various factors. Further research revealed SLC1A5 as a factor involved in receptor localization and viral entry, and co-expression with hACE2 improved the prediction of SARS-CoV-2 binding and entry. The Omicron variant showed higher efficiency in binding to hACE2 sites, but was equally sensitive to SLC1A5 downregulation.
Review
Immunology
Matteo Iannacone, Luca G. Guidotti
Summary: HBV is a hepatotropic virus that can cause persistent infection and serious liver diseases. Immune responses, particularly CD8(+) T cells, play a crucial role in viral clearance and liver pathology.
NATURE REVIEWS IMMUNOLOGY
(2022)