Article
Multidisciplinary Sciences
Lihao Ge, Wonchul Shin, Gianvito Arpino, Lisi Wei, Chung Yu Chan, Christopher K. E. Bleck, Weidong Zhao, Ling-Gang Wu
Summary: This study revealed the existence of sequential compound fusion and compound kiss-and-run mechanisms during vesicle fusion in excitable cells, which enhance exocytosis and endocytosis capacities, as well as dynamic ranges.
Article
Medicine, Research & Experimental
Alexey M. Petrov, Guzalia F. Zakirjanova, Irina Kovyazina, Andrei N. Tsentsevitsky, Ellya A. Bukharaeva
Summary: This study investigated the influence of nerve activity and adrenoceptor stimulation on exocytosis modes in the motor nerve terminal. It was found that high frequency activity promoted neurotransmitter release via kiss-and-run mechanism, while noradrenaline and alpha 2-adrenoreceptors agonist favored full-collapse exocytosis. This has implications for the regulation of respiratory system during stress.
Article
Biochemistry & Molecular Biology
Matheus C. Bianchini, Luiz F. W. Soares, Joao M. F. M. Sousa, Bruna P. Ramborger, Mateus C. Gayer, Jessika C. Bridi, Rafael Roehrs, Simone Pinton, Michael Aschner, Daiana S. Avila, Robson L. Puntel
Summary: This study found that exposure to methylmercury in fruit flies led to reduced survival rate, decreased levels of dopamine and octopamine, and changes in behavior. These effects were associated with inhibition of acetylcholinesterase and tyrosine hydroxylase, increased oxidative stress, and altered glutathione-S-transferase activity.
CHEMICO-BIOLOGICAL INTERACTIONS
(2022)
Article
Neurosciences
Wonchul Shin, Lisi Wei, Gianvito Arpino, Lihao Ge, Xiaoli Guo, Chung Yu Chan, Edaeni Hamid, Oleg Shupliakov, Christopher K. E. Bleck, Ling-Gang Wu
Summary: This study reveals that endocytosis is mainly mediated by calcium-triggered and dynamin-mediated closure of Omega profiles and fusion pores, leading to the formation of Lambda-shaped, Omega-shaped, and O-shaped vesicles, rather than the traditional flat-to-round membrane transformation.
Article
Neurosciences
Silas A. Buck, Briana R. De Miranda, Ryan W. Logan, Kenneth N. Fish, J. Timothy Greenamyre, Zachary Freyberg
Summary: The study found that DA neurons expressing VGLUT2 in the VTA/SNc exhibit higher resilience to rotenone-induced neurodegeneration, with an increase in VGLUT2 expression in response to rotenone. Dopaminergic terminals with VGLUT2 expression show greater protection throughout the striatum.
JOURNAL OF NEUROSCIENCE
(2021)
Article
Neurosciences
Yuxiao Yao, Diego Baronio, Yu-Chia Chen, Congyu Jin, Pertti Panula
Summary: This study found that the absence of the hrh1 gene in zebrafish leads to deficits in the dopaminergic and hypocretin systems during early development, but these deficits are compensated for in adulthood. However, adult fish exhibit impaired sociability and anxious-like behavior, along with downregulation of choline O-acetyltransferase and LIM homeodomain transcription factor Islet1.
MOLECULAR NEUROBIOLOGY
(2023)
Article
Chemistry, Physical
Ary Lautaro Di Bartolo, Claudia N. Tomes, Luis S. Mayorga, Diego Masone
Summary: This work reveals the importance of the C2B domains in the expansion of fusion pores and their ability to decrease the occurrence of kiss-and-run events.
JOURNAL OF CHEMICAL THEORY AND COMPUTATION
(2022)
Article
Biochemistry & Molecular Biology
Zhi Dong Zhou, Wuan Ting Saw, Patrick Ghim Hoe Ho, Zhi Wei Zhang, Li Zeng, Ya Yin Chang, Alfred Xu Yang Sun, Dong Rui Ma, Hong Yan Wang, Lei Zhou, Kah Leong Lim, Eng-King Tan
Summary: This study found that the TH-DA pathway plays a critical role in the pathogenesis of Parkinson's disease. LRRK2 and PINK1 have opposing effects on the TH-DA pathway, and the balance between them affects the survival of DA neurons. Mutations in LRRK2 or PINK1 can disrupt this balance, leading to the demise of DA neurons.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Biology
Sage S. Frehner, Kip T. Dooley, Michelle C. Palumbo, Aaron L. Smith, Mark M. Goodman, Karen L. Bales, Sara M. Freeman
Summary: This study found significantly lower levels of OXTR in females with autism compared to other groups. There were no differences in mRNA levels for either gene across the four groups. These results suggest that dysregulation in OXTR protein translation or increased OXTR internalization/recycling may contribute to the differences in social symptoms seen in females with autism.
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2022)
Article
Neurosciences
Mohamad Ayajuddin, Rahul Chaurasia, Abhik Das, Priyanka Modi, Limamanen Phom, Zevelou Koza, Sarat Chandra Yenisetti
Summary: Parkinson's disease is characterized by the loss of dopaminergic neurons in the substantia nigra pars compacta. Using a fly model, our laboratory has shown that there is a reduction in fluorescence intensity of secondary antibodies targeting tyrosine hydroxylase, indicating a decrease in dopamine synthesis and dysfunction of dopaminergic neurons. This quantification method of fluorescence intensity can be a sensitive and cost-effective approach for studying neurodegenerative diseases.
FRONTIERS IN NEUROSCIENCE
(2023)
Article
Pharmacology & Pharmacy
Weimin Kong, Xueying Sun, Siyu Yu, Peihua Liu, Xiaoke Zheng, Jiaxin Zhang, Liang Zhu, Tianxin Jiang, Mengmeng Jin, Jinghui Gao, Xiaomin Fan, Xiaodong Liu, Li Liu
Summary: Liver failure is associated with increased levels of dopamine in the brain, which can lead to psychiatric alterations. This study investigated the relationship between dopamine levels and mental abnormalities using BDL rats and SH-SY5Y cells, and explored the mechanism by which liver failure increases dopamine levels.
BRITISH JOURNAL OF PHARMACOLOGY
(2023)
Article
Medicine, Research & Experimental
Alba Tristan-Noguero, Irene Fernandez-Carasa, Carles Calatayud, Cristina Bermejo-Casadesus, Meritxell Pons-Espinal, Arianna Colini Baldeschi, Leticia Campa, Francesc Artigas, Analia Bortolozzi, Rosario Domingo-Jimenez, Salvador Ibanez, Merce Pineda, Rafael Artuch, Angel Raya, Angels Garcia-Cazorla, Antonella Consiglio
Summary: This study used induced pluripotent stem cells (iPSCs) to generate dopaminergic neurons (DAn) from patients with tyrosine hydroxylase deficiency (THD) and compared them with control-DAn. THD iPSC-DAn showed lower levels of dopamine metabolites and reduced TH expression compared to controls. Additionally, THD iPSC-DAn displayed abnormal morphology and neuronal defects, which were rescued by L-Dopa treatment only in the milder form of THD (THD-A). Treatment at the stage of neuronal precursors could prevent the alterations in the severe form of THD (THD-B). This iPSC-based model recapitulates THD disease phenotypes and response to treatment.
EMBO MOLECULAR MEDICINE
(2023)
Article
Peripheral Vascular Disease
Laia Canes, Judith Alonso, Carme Ballester-Servera, Saray Varona, Jose R. Escudero, Vicente Andres, Cristina Rodriguez, Jose Martinez-Gonzalez
Summary: The upregulation of the sympathetic nervous system pathway involving tyrosine hydroxylase (TH) may play a critical role in the pathophysiology of AAA. Pharmacological strategies targeting TH show potential for the management of AAA by normalizing vascular Th expression and preserving vascular integrity.
Article
Biochemistry & Molecular Biology
Laura M. Vecchio, Patricia Sullivan, Amy R. Dunn, Marie Kristel Bermejo, Rong Fu, Shababa T. Masoud, Emil Gregersen, Nikhil M. Urs, Reza Nazari, Poul Henning Jensen, Amy Ramsey, David S. Goldstein, Gary W. Miller, Ali Salahpour
Summary: TH plays a crucial role in Parkinson's disease, increasing TH activity leads to oxidative stress and accumulation of toxic dopamine metabolites in vivo, augmenting toxicity.
JOURNAL OF NEUROCHEMISTRY
(2021)
Article
Agronomy
Shimin Chen, Satyabrata Nanda, Mujuan Guo, Lan Kong, Chunxiao Yang, Zhuoqi Liu, Ran Gao, Baoli Qiu, Youjun Zhang, Xuguo Zhou, Huipeng Pan
Summary: The role of tyrosine hydroxylase (HvTH) in the 28-spotted potato ladybeetle (Henosepilachna vigintioctopunctata) was investigated using dietary RNA interference (RNAi) and hematoxylin and eosin (H&E) staining. The results showed that HvTH is involved in larval-pupal-adult cuticle melanization, sclerotization, and reproduction. Ingestion of dsHvTH led to cuticle tanning impairment and larval feeding arrest, resulting in 100% mortality. HvTH inhibition disrupted cuticle tanning and prevented pupation. HvTH silencing in adult females reduced offspring hatching rates.
PEST MANAGEMENT SCIENCE
(2022)
Article
Neurosciences
Daniela B. Pereira, Yvonne Schmitz, Jozsef Meszaros, Paolomi Merchant, Gang Hu, Shu Li, Adam Henke, Jose Lizardi-Ortiz, Richard J. Karpowicz, Travis J. Morgenstern, Mark S. Sonders, Ellen Kanter, Pamela C. Rodriguez, Eugene V. Mosharov, Dalibor Sames, David Sulzer
NATURE NEUROSCIENCE
(2016)
Article
Neurosciences
Natalia Assaife-Lopes, Vasco C. Sousa, Daniela B. Pereira, Joaquim A. Ribeiro, Moses V. Chao, Ana M. Sebastiao
JOURNAL OF NEUROSCIENCE
(2010)
Correction
Neurosciences
Natalia Assaife-Lopes, Vasco C. Sousa, Daniela B. Pereira, Joaquim A. Ribeiro, Moses V. Chao, Ana M. Sebastiao
JOURNAL OF NEUROSCIENCE
(2013)
Article
Multidisciplinary Sciences
Pamela C. Rodriguez, Daniela B. Pereira, Anders Borgkvist, Minerva Y. Wong, Candace Barnard, Mark S. Sonders, Hui Zhang, Dalibor Sames, David Sulzer
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2013)
Article
Biochemistry & Molecular Biology
Natalia Assaife-Lopes, Vasco C. Sousa, Daniela B. Pereira, Joaquim A. Ribeiro, Ana M. Sebastiao
PURINERGIC SIGNALLING
(2014)
Article
Multidisciplinary Sciences
Niko G. Gubernator, Hui Zhang, Roland G. W. Staal, Eugene V. Mosharov, Daniela B. Pereira, Minerva Yue, Vojtech Balsanek, Paul A. Vadola, Bipasha Mukherjee, Robert H. Edwards, David Sulzer, Dalibor Sames
Correction
Biochemistry & Molecular Biology
Margarida V. Caldeira, Carlos V. Melo, Daniela B. Pereira, Ricardo Carvalho, Susana S. Correia, Donald S. Backos, Ana Luisa Carvalho, Jose A. Esteban, Carlos B. Duarte
JOURNAL OF BIOLOGICAL CHEMISTRY
(2007)
Article
Neurosciences
Margarida V. Caldeira, Carlos V. Melo, Daniela B. Pereira, Ricardo F. Carvalho, Ana Luisa Carvalho, Carlos B. Duarte
MOLECULAR AND CELLULAR NEUROSCIENCE
(2007)
Article
Neurosciences
Daniela B. Pereira, Moses V. Chao
JOURNAL OF NEUROSCIENCE
(2007)
Article
Biochemistry & Molecular Biology
Margarida V. Caldeira, Carlos V. Melo, Daniela B. Pereira, Ricardo Carvalho, Susana S. Correia, Donald S. Backos, Ana Luisa Carvalho, Jose A. Esteban, Carlos B. Duarte
JOURNAL OF BIOLOGICAL CHEMISTRY
(2007)
Article
Neurosciences
DB Pereira, N Rebola, RJ Rodrigues, RA Cunha, AP Carvalho, CB Duarte
JOURNAL OF NEUROSCIENCE RESEARCH
(2006)
Article
Biochemistry & Molecular Biology
JC Arévolo, DB Pereira, H Yano, KK Teng, MV Chao
JOURNAL OF BIOLOGICAL CHEMISTRY
(2006)
Letter
Neurosciences
DB Pereira, CB Duarte
Article
Biochemistry & Molecular Biology
DB Pereira, AP Carvalho, CB Duarte
NEUROCHEMISTRY INTERNATIONAL
(2003)