Article
Agriculture, Multidisciplinary
Jiaxu Wang, Boyue Shao, Jiaxin Li, Zhimin Wang, Mixia Zhang, Lili Jia, Pengfei Yu, Chunli Ma
Summary: This study evaluated the production of ACE-inhibitory peptides during the fermentation of skimmed milk by Lacticaseibacillus paracasei M3 strain and investigated their inhibitory mechanism and stability. The fermented milk showed ACE inhibition activity of 71.94±1.39%. After digestion, the ACE inhibitory activities of the fermented milk were 78.40±1.93% and 74.96±1.73% by gastric juice and pancreatic juice, respectively. Bioinformatics analysis identified 11 peptides from milk proteins, and molecular docking revealed a high affinity between peptide PWIQPK and ACE. In silico proteolysis suggested that PWIQPK is relatively stable in the digestive tract. These findings suggest that milk fermented by L. paracasei M3 has the potential to be used as a functional food with antihypertensive effects.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Review
Pharmacology & Pharmacy
Michael J. Davies
Summary: Herne peroxidases are a major source of reactive oxidants involved in immune response, tissue development, and hormone synthesis. Improper production of these oxidants can lead to tissue damage and is associated with various inflammatory pathologies.
PHARMACOLOGY & THERAPEUTICS
(2021)
Article
Biochemistry & Molecular Biology
I. V. Gorudko, D. V. Grigorieva, E. V. Shamova, N. P. Gorbunov, A. U. Kokhan, V. A. Kostevich, V. B. Vasilyev, O. M. Panasenko, N. V. Khinevich, H. V. Bandarenka, A. A. Burko, A. V. Sokolov
Summary: Myeloperoxidase (MPO), an oxidant-producing enzyme, exists in various forms in inflammatory foci and can interact with other molecules. In this study, we compared the effects of different forms of MPO on platelet activity. We found that monomeric MPO had a stronger effect on platelet activity compared to dimeric MPO. MPO modified by HOCl and MPO in complex with CP did not affect platelet activity.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2022)
Article
Biochemistry & Molecular Biology
Saniya Arfin, Kirti Agrawal, Sujata Maurya, Shailendra Asthana, Dario Di Silvestre, Dhruv Kumar
Summary: In silico docking studies were used to identify potential inhibitors for the CP-Myeloperoxidase (MPO) interaction site. Lycoperoside F derived from tomatoes and Xyloglucan (XG) oligosaccharides from plants showed high binding affinity with CP, indicating their potential use as strong drug candidates. Ardimerin digallate, known as a retroviral ribonuclease H inhibitor, also displayed favorable interactions. These phytochemicals have the potential to progress to in vitro studies as CP inhibitors.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Review
Chemistry, Physical
Marcos Lopez Hernandez, Jan Skov Pedersen, Daniel E. Otzen
Summary: The synergies between surfactants and proteins are widely utilized in various fields, but there is still room for improvement in terms of sustainability. Biosurfactants offer an attractive alternative, and understanding their interactions with proteins can open up new opportunities. This review focuses on the self-assembly of protein:BS complexes and the effects of BS on enzymatic activity.
CURRENT OPINION IN COLLOID & INTERFACE SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Whendy Contreras, J. Fernando Bazan, Torben Mentrup
Summary: F rey1 has been identified as an inhibitory protein of SPPL2c, and its specific motif can be transplanted to the substrate PLN to generate inhibitors for this enzyme. The findings suggest a mechanism for inhibiting aspartic I-CLIPs and provide potential tools for targeted drug development.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Chemistry, Applied
Da Chen, Osvaldo H. Campanella
Summary: In this study, pea protein hydrolysates were obtained through enzymatic hydrolysis, and their physicochemical properties and gelling capacity were investigated. The hydrolysates showed decreased fluorescence intensity and surface hydrophobicity compared to the original protein solution, indicating increased water accessibility and polarity. The hydrolysates were unstable at high temperatures but formed gels under specific conditions. Addition of certain substances decreased the gel strength, suggesting the involvement of electrostatic and hydrophobic interactions, as well as hydrogen and disulfide bonds in gelation.
FOOD HYDROCOLLOIDS
(2022)
Article
Pharmacology & Pharmacy
Xiaoxi Lv, Shanshan Liu, Chang Liu, Yunxuan Li, Tingting Zhang, Jie Qi, Ke Li, Fang Hua, Bing Cui, Xiaowei Zhang, Yuxin Liu, Jiaojiao Yu, Jinmei Yu, Li Li, Xia Li, Zhigang Yao, Bo Huang
Summary: This study reveals that repetitive lung damage leads to the accumulation of the transcriptional repressor SLUG, inhibiting lung alveolar regeneration and causing the failure of pulmonary fibrosis. The elevated SLUG represses the expression of phosphate transporter SLC34A2, reducing intracellular phosphate and inhibiting key kinases JNK and P38 MAPK, resulting in LAR failure. Targeting the TRIB3/MDM2 interaction can restore LAR capacity and provide potential therapeutic efficacy against fibroproliferative lung diseases.
ACTA PHARMACEUTICA SINICA B
(2023)
Article
Plant Sciences
Ismail Dahmani, Kezhen Qin, Youjun Zhang, Alisdair R. Fernie
Summary: Metabolons are temporary structural-functional complexes of sequential enzymes in a metabolic pathway. In plants, only four substrate channels have been demonstrated. This study provides an overview of these four metabolons and discusses methodologies to enhance our knowledge of plant metabolons that assemble via different mechanisms, including liquid droplet phase separation and enzyme chemotaxis. Novel approaches based on subcellular-level mass spectral imaging, proteomics, and structural and computational biology are also proposed.
Article
Food Science & Technology
Hao Zhu, Yi Zhang, Tao Yang, Dongyun Zheng, Xiaojun Liu, Juntao Zhang, Mingming Zheng
Summary: Metal affinity-based enzyme immobilization was studied with different metal ions chelated with triamino ligands on Alcalase-immobilized hollow mesoporous silica spheres. Transition metal ions exhibited easier chelation with the ligands. Immobilized Alcalase showed sustained activity after multiple cycles of reuse.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Tetsuya Sakajiri, Masatoshi Nakatsuji, Yoshiaki Teraoka, Kosuke Furuta, Katsuya Ikuta, Kotoe Shibusa, Eriko Sugano, Hiroshi Tomita, Takashi Inui, Takaki Yamamura
Summary: The study investigates the interaction mechanism between ceruloplasmin and transferrin mediated by zinc. The findings suggest that zinc may play a role in the transfer of iron between ceruloplasmin and transferrin.
Review
Biochemistry & Molecular Biology
Yuqing Lu, Yuewen Yang, Guanghao Zhu, Hairong Zeng, Yiming Fan, Fujia Guo, Dongshu Xu, Boya Wang, Dapeng Chen, Guangbo Ge
Summary: Targeted therapies in cancer treatment aim to improve treatment efficacy and reduce side effects by altering the exposure of specific biomolecules. However, many target proteins in cancer remain undruggable due to factors such as lack of binding pockets, protein-protein interactions, conserved active sites, and structural variability. This review discusses the current status of undruggable targets and explores novel techniques and drug designing strategies, including proteolysis targeting chimeras (PROTACs), protein degradation, protein-protein interactions, intrinsically disordered regions, and protein-DNA binding. Furthermore, the use of intelligence-assisted technologies like Alpha-Fold for protein structure prediction and the development of new drug development strategies are crucial for overcoming these undruggable targets.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Junjia Luan, Lei Wang, Junnan Yang, Xuepeng Li, Jinxiang Wang, Fangchao Cui, Hongbo Mi, Yongxia Xu, Shumin Yi, Jianrong Li
Summary: Novel antioxidant peptides were identified from Pacific saury, and their structures and properties were analyzed. These peptides showed strong antioxidant activity and protective effects against oxidative damage in cells.
Article
Food Science & Technology
Zhengli Lin, Junwen Lai, Ping He, Leiman Pan, Yizhe Zhang, Mengmeng Zhang, Hui Wu
Summary: In this study, six novel ACE inhibitor peptides were identified in maca protein hydrolysates, and RSRGVFF exhibited prominent ACE inhibitory activity. The analysis of the structure-activity relationship revealed that arginine at the N-terminal and two phenylalanines at the C-terminal contributed to the inhibitory activity. These findings suggest that maca protein may play a role in the antihypertensive activity.
Article
Biochemistry & Molecular Biology
Shifeng Xiao, Qiuping Wu, Xuanbao Yao, Jiahao Zhang, Weicong Zhong, Junyi Zhao, Qiong Liu, Mohan Zhang
Summary: Isobavachalcone is a promising compound for potential preventive and therapeutic medicine for Alzheimer's disease, as it can inhibit tau protein aggregation and disaggregation, as well as regulate tau phosphorylation.
ACS CHEMICAL NEUROSCIENCE
(2021)
Article
Virology
Alexey Sokolov, Irina Isakova-Sivak, Natalia Grudinina, Daria Mezhenskaya, Elena Litasova, Valeria Kostevich, Ekaterina Stepanova, Alexandra Rak, Ivan Sychev, Olga Kirik, Larisa Rudenko
Summary: The degradation of transferrin receptor 1 (TfR1) by ferristatin II inhibits the entry and replication of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). This finding suggests a promising therapeutic strategy for COVID-19.
Article
Food Science & Technology
Peter V. Dubovskii, Kira M. Dubova, Gleb Bourenkov, Vladislav G. Starkov, Anastasia G. Konshina, Roman G. Efremov, Yuri N. Utkin, Valeriya R. Samygina
Summary: This study determined the spatial structures of two cytotoxins from cobra venom and found that these toxins undergo conformational changes during interaction with lipid membranes.
Article
Nutrition & Dietetics
Alexey Sokolov, Nadezhda M. Dubrovskaya, Valeria A. Kostevich, Dmitrii S. Vasilev, Irina V. Voynova, Elena T. Zakharova, Olga L. Runova, Igor Semak, Alexander Budevich, Natalia N. Nalivaeva, Vadim B. Vasilyev
Summary: The protective effects of apo-rhLF on the cognitive functions of rat offspring subjected to prenatal hypoxia have been analyzed. The results showed that apo-rhLF can rescue cognitive deficits caused by prenatal hypoxia and improve memory.
Article
Crystallography
K. M. Dubova, A. V. Vlaskina, D. A. Korzhenevskiy, Yu. K. Agapova, T. V. Rakitina, V. R. Samygina
Summary: This study aims to determine the crystal structure of the envelope (E) protein ectodomain of Far-Eastern tick-borne encephalitis virus subtype (Sofjin strain) in order to develop specific inhibitors of conformational rearrangements that are essential for the initial stages of infection.
CRYSTALLOGRAPHY REPORTS
(2022)
Review
Biochemistry & Molecular Biology
V. E. Reut, I. V. Gorudko, D. V. Grigorieva, A. V. Sokolov, O. M. Panasenko
Summary: Hypochlorous acid (HOCl) plays a crucial role in the immune system but its high reactivity can also lead to the development of diseases. Therefore, developing highly sensitive and selective HOCl biosensors is important. This review summarizes the achievements in the development of chemosensors for HOCl detection in biological systems from 2016 to 2021. The challenges in designing and applying small-molecule probes are discussed, and recommendations are given for their future application.
RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY
(2022)
Article
Biochemical Research Methods
Vitaly G. Grigorenko, Tatiana E. Petrova, Ciaran Carolan, Maya Yu Rubtsova, Igor Uporov, Joana Pereira, Grzegorz Chojnowski, Valeriya R. Samygina, Victor S. Lamzin, Alexey M. Egorov
Summary: This study presents novel crystal structures of the native beta-lactamase TEM-171 and its complexes with the inhibitor tazobactam. The results provide valuable insights for the rational design of new beta-lactamase inhibitors.
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Veronika E. Reut, Stanislav O. Kozlov, Igor Kudryavtsev, Natalya A. Grudinina, Valeria A. Kostevich, Nikolay P. Gorbunov, Daria Grigorieva, Julia A. Kalvinkovskaya, Sergey B. Bushuk, Elena Yu Varfolomeeva, Natalia D. Fedorova, Irina Gorudko, Oleg M. Panasenko, Vadim B. Vasilyev, Alexey Sokolov
Summary: This paper introduces a fluorescent probe called Celestine Blue B (CB), which can be used for HOCl detection in living cells and for assaying the chlorinating activity of MPO. The probe shows remarkable selectivity and sensitivity, and has a rapid response to HOCl. It can also detect endogenous HOCl and reactive halogenated species using various techniques.
Article
Biochemistry & Molecular Biology
A. Sokolov, I. N. Isakova-Sivak, D. A. Mezhenskaya, V. A. Kostevich, N. P. Gorbunov, A. Yu Elizarova, V. A. Matyushenko, Yu M. Berson, N. A. Grudinina, N. N. Kolmakov, Y. A. Zabrodskaya, A. S. Komlev, I. Semak, A. Budevich, L. G. Rudenko, V. B. Vasilyev
Summary: The pathogenesis of SARS-CoV-2 infection involves dysregulations of iron metabolism. Spike protein competes with transferrin receptor 1 (TfR1) ligands and shows cross-reactivity with lactoferrin (Lf) and transferrin (Tf). Apo-Lf and holo-Lf can inhibit SARS-CoV-2 replication and protect against COVID-19-induced pathogenesis.
Article
Biochemistry & Molecular Biology
Oleg M. Panasenko, Viktor A. Ivanov, Elena V. Mikhalchik, Irina V. Gorudko, Daria V. Grigorieva, Liliya Yu. Basyreva, Ekaterina V. Shmeleva, Sergey A. Gusev, Valeria A. Kostevich, Nikolay P. Gorbunov, Alexey V. Sokolov
Summary: Hyperglycemia in diabetes mellitus alters the functionality of neutrophils, reducing their bactericidal activity mediated by MPO, which is affected by the modification of proteins by glucose and its derivative methylglyoxal. This phenomenon may contribute to impaired wound healing and increased susceptibility to infection in patients with hyperglycemia.
Article
Biochemistry & Molecular Biology
Tatyana V. Vakhrusheva, Alexey V. Sokolov, Grigoriy D. Moroz, Valeria A. Kostevich, Nikolay P. Gorbunov, Igor P. Smirnov, Ekaterina N. Grafskaia, Ivan A. Latsis, Oleg M. Panasenko, Vassili N. Lazarev
Summary: Cationic antimicrobial peptides (CAMPs) have potential as antimicrobial therapeutics with low bacterial resistance. However, their interaction with biologically important proteins lacks research. This study found that CAMPs may have a dual role as antimicrobial agents and regulators of MPO activation.
Article
Biochemistry & Molecular Biology
Elena V. Mikhalchik, Liliya N. Maltseva, Roxalana K. Firova, Marina A. Murina, Irina V. Gorudko, Daria V. Grigorieva, Viktor A. Ivanov, Ekaterina A. Obraztsova, Dmitry V. Klinov, Ekaterina V. Shmeleva, Sergey A. Gusev, Oleg M. Panasenko, Alexey V. Sokolov, Nikolay P. Gorbunov, Lyubov Y. Filatova, Nadezhda G. Balabushevich
Summary: This study investigated the effect of incorporating pectin into vaterite microparticles on cytotoxicity and mucin-dependent neutrophil activation. The results showed that the inclusion of pectin did not cause damage to neutrophils and did not have direct pro- or antioxidant effects. However, when neutrophils were activated by mucin-treated microparticles, the incorporation of pectin into vaterite microspheres enabled an antioxidant effect to be reached, presumably via exposed ligands.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Eleonora A. Starikova, Jennet T. Mammedova, Arina Ozhiganova, Tatiana A. Leveshko, Aleksandra M. Lebedeva, Alexey V. Sokolov, Dmitry V. Isakov, Alena B. Karaseva, Larissa A. Burova, Igor V. Kudryavtsev
Summary: In this study, the impact and mechanism of streptococcal arginine deiminase (ADI) on the function of human peripheral blood T lymphocytes were investigated. The results showed that ADI decreased arginine concentration, suppressed immune cell activity and altered cell differentiation state, as well as inhibited autophagy processes.
Correction
Crystallography
K. M. Dubova, A. V. Vlaskina, D. A. Korzhenevskiy, Yu. K. Agapova, T. V. Rakitina, V. R. Samygina
CRYSTALLOGRAPHY REPORTS
(2022)
Article
Crystallography
A. V. Sokolov, V. B. Vasilyev, V. R. Samygina
Summary: This study successfully obtained human ceruloplasmin crystals and determined the conformation of a labile functional loop between two structural domains. By comparing different crystal packings, the efficiency of the protein purification method was confirmed, along with the importance of loop integrity in neutrophil myeloperoxidase inhibition during complex formation with ceruloplasmin.
CRYSTALLOGRAPHY REPORTS
(2022)
