Article
Genetics & Heredity
Matthew D. Lynes, Qian Huang, Carolina Cora, Sheng-Chiang Su, Peng Yi, Yu-Hua Tseng
Summary: This study reveals the complexity of genetic regulation of brown adipogenesis and glucose metabolism. The E3-ubiquitin ligase Rfwd2 was found to suppress glucose uptake in brown adipocytes.
Article
Multidisciplinary Sciences
Adam J. M. Wollman, Dimitrios Kioumourtzoglou, Rebecca Ward, Gwyn W. Gould, Nia J. Bryant
Summary: Fluorescent biosensors are powerful tools for measuring metabolites and other properties inside live single cells, but lack of simple software has hindered their use. We have developed a new software package, FRETzel, which allows for the identification and quantification of biosensor signals in single cells. By using FRETzel, we were able to measure insulin-stimulated glucose uptake in individual fat cells of varying sizes, supporting the hypothesis that larger fat cells are less sensitive to insulin. FRETzel has been optimized for use in different cell types and provides a user-friendly interface for quantifying FRET biosensors.
Article
Biochemistry & Molecular Biology
Tomomi Minemura, Atsunori Fukuhara, Michio Otsuki, Iichiro Shimomura
Summary: The study reveals that LDHA regulates Glut1 expression and basal glucose uptake in adipocytes. Knockout or inhibition of LDHA leads to decreased Glut1 protein levels and reduced glucose uptake.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Yan-Bo Kou, Xiao-Qing Yan, Qi-Yue Jing, Sheng-Han Zhang, Zhuan-Zhuan Liu, Yan-Xia Wei, Yu-Gang Wang
Summary: The study showed that the LIGHT-LTβR-AKT-GLUT4 axis plays a crucial role in regulating glucose uptake in adipose tissue, highlighting the importance of LIGHT in maintaining glucose homeostasis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Seo Woo Nam, Min Seuk Kim, Younho Han, Kwang Youl Lee
Summary: The study shows that WJCPR11 has great potential for improving insulin resistance and diabetes treatment by promoting adipocyte differentiation, increasing the levels of adipogenic markers, and reducing the expression of inflammatory markers.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Noemi Esteras, Thomas S. Blacker, Evgeny A. Zherebtsov, Olga A. Stelmashuk, Ying Zhang, W. Christian Wigley, Michael R. Duchen, Albena T. Dinkova-Kostova, Andrey Y. Abramov
Summary: The transcription factor Nrf2 and its repressor Keap1 play a crucial role in cell stress adaptation by regulating gene expression related to cellular detoxification, antioxidant defense, and energy metabolism. This study investigated the impact of Nrf2 on glucose distribution and the connection between NADH production in energy metabolism and NADPH homeostasis using glio-neuronal cultures. The findings showed that Nrf2 activation enhances glucose uptake in neurons and astrocytes, with prioritized consumption for mitochondria-related energy production rather than NADPH synthesis in the pentose phosphate pathway.
Review
Endocrinology & Metabolism
Jonathan S. S. Bogan
Summary: This article reviews recent advances in the mechanism of GLUT4 glucose transporter mobilization to enhance glucose uptake in response to insulin stimulation. The processing of TUG proteins plays a central role in regulating this process, with endoproteolytic cleavage of TUG mediated by the Usp25m protease being a key step in vesicle release. TUG processing also affects gene expression related to fatty acid oxidation and thermogenesis, further regulating energy metabolism.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Chemistry, Organic
Zeyi Li, Tingting Sun, Jiaxiang Guo, Xinyu Tian, Yujia Liu, Shihou Sheng, Kaiqi Ye, Zhigang Xie, Chuandong Dou
Summary: The study reports two ladder-type boron-containing π-conjugated molecules with enhanced singlet diradical characteristic and partial contributions of tetraradical states. They exhibit attractive physicochemical properties including magnetic activities, reversible redox activity, narrow band gaps, and remarkable near-infrared light absorption properties. Moreover, the nanoparticles based on them show intriguing photothermal conversion properties and excellent photostability, suggesting their potential application as organic photothermal materials.
ORGANIC CHEMISTRY FRONTIERS
(2023)
Article
Engineering, Environmental
Chunyan Chen, Yao Lu, Jieying Liang, Liping Wang, Jingyun Fang
Summary: The role of nitrite (NO2-) in the transformation of aniline by sulfate radicals (SO4•-) and hydroxyl radicals (HO•) was investigated in this study. It was found that NO2- played an important catalytic role in both SO4•- and HO• systems, contributing to the overall degradation rates of aniline.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Chemistry, Multidisciplinary
Xuan Cui, Haojie Liu, Ting Shi, Qingwen Zhao, Feiyan Li, Wenjing Lv, Chao Yu, Haiyan Huang, Qi-Qun Tang, Dongning Pan
Summary: This study reveals the important role of IFI27 in maintaining mitochondrial morphology and function in brown adipocytes. IFI27 interacts with SDHB and HADHA proteins, and protects SDHB from oxidative damage-induced degradation by linking it to TRAP1. Additionally, IFI27 enhances the catalytic activity of HADHA in the β-oxidation pathway. Loss of IFI27 leads to reduced SDH levels and impaired fatty acid oxidation, resulting in defective oxygen consumption and thermogenesis in brown fat. Overall, IFI27 is a novel regulator of mitochondrial metabolism and thermogenesis.
Article
Pharmacology & Pharmacy
Shiqiao Peng, Xiaoying Zhang, Lili Yu, Yanhong Xu, Yang Zhou, Shengnan Qian, Xinyu Cao, Xiaotong Ye, Jiajun Yang, Weiping Jia, Jianping Ye
Summary: This study investigated the role of the transcription factor nuclear factor of kappa-light-chain-enhancer of activated B cells (NF-kappa B) in brown adipocytes. The findings showed that NF-kappa B activity increased during brown adipocyte differentiation and cold stimulation, protecting the cells from apoptosis by down-regulating the expression of adenine nucleotide translocase 2 (ANT2).
ACTA PHARMACEUTICA SINICA B
(2022)
Article
Cell Biology
Kornel Z. Varga, Katalin Gyurina, Adam Radvanyi, Tibor Pal, Laszlo Sasi-Szabo, Haidong Yu, Eniko Felszeghy, Tamas Szabo, Tamas Roeszer
Summary: Innate immune signaling in adipocytes can affect systemic metabolism. DNA sensing in the cytoplasm of adipocytes can stimulate thermogenic adipocyte differentiation and protect against obesity. However, DNA efflux from adipocyte mitochondria may lead to tissue dysfunction and insulin resistance. This study found that the signal transduction molecule STING can trigger autophagy in adipocytes, thereby protecting them from excessive IFN-I response.
Article
Physiology
Eric D. Queathem, Maggie Fitzgerald, Rebecca Welly, Candace C. Rowles, Kylie Schaller, Shahad Bukhary, Christopher P. P. Baines, R. Scott Rector, Jaume Padilla, Camila Manrique-Acevedo, Dennis B. Lubahn, Victoria J. Vieira-Potter
Summary: Dysfunction of white adipose tissue (WAT) is a predictor of cardiometabolic disease, and current therapeutics targeting adipocytes are not effective. This study found that estrogen receptor (ER) beta may enhance the response of WAT to B3AR agonists. Understanding the role of ER beta in adipocyte metabolism could have important clinical applications.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Mara Fiorani, Rita De Matteis, Barbara Canonico, Giulia Blandino, Alessandro Mazzoli, Mariele Montanari, Andrea Guidarelli, Orazio Cantoni
Summary: The conversion of human SW872 preadipocytes to mature adipocytes involves time-dependent changes in differentiation markers' expression, morphological changes, and accumulation of lipid droplets. Reactive oxygen species (ROS) formation was significant at specific time points during differentiation, with NADPH oxidase (NOX)-2 playing a role. Mitochondrial ROS (mROS) were only detected in the late phase of differentiation, indicating potential mitochondrial dysfunction. These findings suggest a potential link between ROS, adipogenesis, and adipose tissue dysfunction in SW872 cells.
Article
Chemistry, Organic
Zhenhui Wang, Xiaofeng Li, Wei Li, Yongyong Cao, Huaifeng Li
Summary: This study introduces a metal-free reductive acyldifluoroalkylation method for synthesizing β-difluoroalkyl ketones, which can proceed at room temperature. This method overcomes the limitations of previous synthesis methods that required heating or precious metal photocatalysts/oxidants, and it exhibits mild conditions, a broad substrate scope, and late-stage functionalization, facilitated by cooperative NHC and organophotocatalysis.
ORGANIC CHEMISTRY FRONTIERS
(2023)
Review
Toxicology
Kelly M. Quesnelle, Phillip V. Bystrom, Luis H. Toledo-Pereyra
ARCHIVES OF TOXICOLOGY
(2015)
Review
Surgery
Ryan T. Jones, Luis H. Toledo-Pereyra, Kelly M. Quesnelle
JOURNAL OF INVESTIGATIVE SURGERY
(2015)
Article
Cell Biology
Anupriya Khare, Mahesh Raundhal, Krishnendu Chakraborty, Sudipta Das, Catherine Corey, Christelle K. Kamga, Kelly Quesnelle, Claudette St Croix, Simon C. Watkins, Christina Morse, Timothy B. Oriss, Rachael Huff, Rachel Hannum, Prabir Ray, Sruti Shiva, Anuradha Ray
Article
Oncology
Changqing Ma, Kelly M. Quesnelle, Anthony Sparano, Shilpa Rao, Min S. Park, Marc A. Cohen, Yan Wang, Minu Samanta, Madhu S. Kumar, M. Usman Aziz, Tara L. Naylor, Barbara L. Weber, Steven S. Fakharzadeh, Gregory S. Weinstein, Anil Vachani, Michael D. Feldman, Marcia S. Brose
CANCER BIOLOGY & THERAPY
(2009)
Article
Oncology
Kelly M. Quesnelle, Sarah E. Wheeler, Mary K. Ratay, Jennifer R. Grandis
CANCER BIOLOGY & THERAPY
(2012)
Article
Cardiac & Cardiovascular Systems
Christelle Kamga Pride, Li Mo, Kelly Quesnelle, Ruben K. Dagda, Daniel Murillo, Lisa Geary, Catherine Corey, Rafael Portella, Sergey Zharikov, Claudette St Croix, Salony Maniar, Charleen T. Chu, Nicholas K. H. Khoo, Sruti Shiva
CARDIOVASCULAR RESEARCH
(2014)
Article
Oncology
Lynn M. Knowles, Laura P. Stabile, Ann Marie Egloff, Mary E. Rothstein, Sufi M. Thomas, Christopher T. Gubish, Edwina C. Lerner, Raja R. Seethala, Shinsuke Suzuki, Kelly M. Quesnelle, Sarah Morgan, Robert L. Ferris, Jennifer R. Grandis, Jill M. Siegfried
CLINICAL CANCER RESEARCH
(2009)
Correction
Oncology
Lynn M. Knowles
CLINICAL CANCER RESEARCH
(2010)
Article
Oncology
Kelly M. Quesnelle, Jennifer R. Grandis
CLINICAL CANCER RESEARCH
(2011)
Article
Oncology
Laura P. Stabile, Guoqing He, Vivian Wai Yan Lui, Cassandra Henry, Christopher T. Gubish, Sonali Joyce, Kelly M. Quesnelle, Jill M. Siegfried, Jennifer R. Grandis
CLINICAL CANCER RESEARCH
(2013)
Correction
Oncology
L. P. Stabile, G. He, V. W. Lui, S. Thomas, C. Henry, C. T. Gubish, S. Joyce, K. M. Quesnelle, J. M. Siegfried, J. R. Grandis
CLINICAL CANCER RESEARCH
(2013)
Article
Biochemical Research Methods
Wei Yang, Quan Cai, Vivian W. Y. Lui, Patrick A. Everley, Jayoung Kim, Neil Bhola, Kelly M. Quesnelle, Bruce R. Zetter, Hanno Steen, Michael R. Freeman, Jennifer R. Grandis
JOURNAL OF PROTEOME RESEARCH
(2010)
Article
Education, Scientific Disciplines
Kelly M. Quesnelle, David R. Bright, Lisa A. Salvati
CURRENTS IN PHARMACY TEACHING AND LEARNING
(2018)
Review
Biology
Phillip Bystrom, Nicole Foley, Luis Toledo-Pereyra, Kelly Quesnelle
Correction
Biochemistry & Molecular Biology
Siew Chin Chan, Chih-Wei Tung, Chia-Wei Lin, Yun-Shiuan Tung, Po-Min Wu, Pei-Hsun Cheng, Chuan-Mu Chen, Shang-Hsun Yang
FREE RADICAL BIOLOGY AND MEDICINE
(2024)
Article
Biochemistry & Molecular Biology
Suyuan Liu, Meiling Tan, Jiangxue Cai, Chenxuan Li, Miaoxin Yang, Xiaoxiao Sun, Bin He
Summary: This study reveals that the antibiotic doxycycline effectively inhibits NLRP3 inflammasome activation by targeting mitochondrial translation and mtDNA synthesis, offering potential for the treatment of NLRP3-related diseases.
FREE RADICAL BIOLOGY AND MEDICINE
(2024)
Article
Biochemistry & Molecular Biology
Hao Liu, Nana Li, Ge Kuang, Xia Gong, Ting Wang, Jun Hu, Hui Du, Minxuan Zhong, Jiashi Guo, Yao Xie, Yang Xiang, Shengwang Wu, Yiling Yuan, Xinru Yin, Jingyuan Wan, Ke Li
Summary: Protectin D1 (PTD1) improves hepatic steatosis, inflammation and fibrosis in a NASH mouse model by inhibiting the activation of TLR4 downstream signaling pathway, possibly through upregulation of IRAK-M expression, suggesting a potential new treatment for NASH.
FREE RADICAL BIOLOGY AND MEDICINE
(2024)