Article
Biochemistry & Molecular Biology
Petra Mikolcevic, Andrea Hlousek-Kasun, Ivan Ahel, Andreja Mikoc
Summary: ADP-ribosylation is an ancient posttranslational modification present in all kingdoms of life, with crucial roles in eukaryotes but still with limited understanding in bacteria and viruses. Research on this system may provide a fighting advantage in the search for therapeutic targets in the future. The relevance of this subject is highlighted by the current world pandemic caused by a virus dependent on a representative of such a system.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2021)
Article
Biology
C. Maresca, A. Dello Stritto, C. D'Angelo, E. Petti, A. Rizzo, E. Vertecchi, F. Berardinelli, L. Bonanni, A. Sgura, A. Antoccia, G. Graziani, A. Biroccio, E. Salvati
Summary: PARP1 interacts with TRF1 and modifies its DNA affinity, influencing telomere replication and helicase recruitment. This study uncovers a new role for PARP1 as a surveillant of telomere replication, orchestrating protein dynamics at the replication fork.
COMMUNICATIONS BIOLOGY
(2023)
Article
Medicine, Research & Experimental
Jeffrey Wang, Mohamed A. Ghonim, Salome Ibba, Hanh H. Luu, Yucel Aydin, Peter A. Greer, A. Hamid Boulares
Summary: This study reveals that poly(ADP-ribosyl)ation plays a critical role in protecting STAT6 from degradation, and can be synthetically targeted for degradation by inhibiting PARP-1. Additionally, the study identifies STAT6 as a bonafide substrate for chaperone-mediated autophagy in the human Jurkat cell-line.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Review
Oncology
Giulia Pinton, Sara Boumya, Maria Rosa Ciriolo, Fabio Ciccarone
Summary: This review article explores the role of PARP-1 and poly(ADP-ribosyl)ation in gene expression, DNA repair pathways, and genomic stability, including their impact on chromatin remodelling. The article specifically focuses on how PARP-1 directly modifies histone proteins and enzymes involved in DNA/histone epigenetic modifications to shape chromatin structure during transcription and DNA damage response. Understanding the role of poly(ADP-ribosyl)ation in regulating chromatin organization could provide insights into resistance mechanisms to PARP inhibitors and the clinical relevance of combining epigenetic drugs.
Article
Biochemistry & Molecular Biology
Masato Mashimo, Momoko Kita, Arina Uno, Moe Nii, Moe Ishihara, Takuya Honda, Yuka Gotoh-Kinoshita, Atsuo Nomura, Hiroyuki Nakamura, Toshihiko Murayama, Ryoichi Kizu, Takeshi Fujii
Summary: Poly(ADP-ribosyl)ation is a post-translational modification process that transfers poly(ADP-ribose) to proteins. TNKS1/2 have been found to play important roles in neuronal development, promoting neurite outgrowth and synapse formation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Anna Rita Bianchi, Alessandra La Pietra, Valeria Guerretti, Anna De Maio, Teresa Capriello, Ida Ferrandino
Summary: By studying the brains of adult zebrafish exposed to aluminum, we found hyperactivation of poly(ADPribosyl)ation and changes in the synthesis and degradation of poly(ADP-ribose). The highest activity levels of poly(ADP-ribose) synthesis and degradation were observed after 10 and 15 days of exposure. We hypothesize that the activation of poly(ADP-ribose) polymerase (PARP) is related to aluminum-induced DNA damage, while the activation of poly(ADPR) glycohydrolase (PARG) is necessary to avoid the accumulation of poly(ADP-ribose).
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Yaroslava Karpova, Danping Guo, Peter Makhov, Adam M. Haines, Dmitriy A. Markov, Vladimir Kolenko, Alexei V. Tulin
Summary: We have shown that the development of clear cell renal cell carcinoma (ccRCC) is associated with the accumulation of poly(ADPribose) due to increased PARP-1 and decreased PARG levels. Modulation of gene expression is a primary cause for the observed anti-tumor effects, with PARP-1 and PARG enzymes playing a crucial role in regulating pADPr level. Interest in PARP-1 inhibitors has increased due to their recognized antitumor efficacy in disrupting malignancy in ccRCC cells.
Review
Microbiology
Joshua Dowling, Craig L. Doig
Summary: ADP-ribosylation is a reversible protein modification that plays crucial roles in cellular functions. It is thought to be important in Trypanosomatidae parasites during infection. Currently available medications for these infections are outdated and have side effects, and access to them is limited in marginalized communities. Therefore, understanding the molecular mechanisms of infection and targeting ADP-ribosylation may offer new therapeutic options.
Review
Biochemistry & Molecular Biology
N. Maluchenko, D. O. Koshkina, A. Feofanov, V. M. Studitsky, M. P. Kirpichnikov
Summary: Poly(ADP-ribosyl)ation plays a crucial role in cellular metabolism and various cellular processes through complex regulatory mechanisms. Pharmacological correction of PAR levels may provide a novel approach for treating a variety of diseases.
Article
Multidisciplinary Sciences
Fabian Konrath, Michael Willenbrock, Dorothea Busse, Claus Scheidereit, Jana Wolf
Summary: The activation of IKK/NF-kappa B is a crucial process in the DNA damage response, affecting cell-fate decisions and interpathway regulation. This study reveals a pathway structure with two hubs and demonstrates how changes in individual components impact pathway activity. It provides mechanistic understanding relevant for experimental and clinical studies.
Article
Oncology
Emad Matanes, Vanessa M. Lopez-Ozuna, David Octeau, Tahira Baloch, Florentin Racovitan, Amandeep Kaur Dhillon, Roy Kessous, Oded Raban, Liron Kogan, Shannon Salvador, Susie Lau, Walter H. Gotlieb, Amber Yasmeen
Summary: The study showed that PARG inhibition can complement PARP inhibition in the treatment of ovarian cancer, especially in the presence of homologous recombination defects. PARG inhibitor alone or in combination with PARPi or Cisplatin can reduce cell migration and induce cell death.
FRONTIERS IN ONCOLOGY
(2021)
Article
Cell Biology
Konstantin N. Naumenko, Mariya V. Sukhanova, Loic Hamon, Tatyana A. Kurgina, Rashid O. Anarbaev, Aswin Mangerich, David Pastre, Olga I. Lavrik
Summary: Y-box-binding protein 1 (YB-1) is involved in the regulation of gene expression and has been found to play a role in the regulation of PARP1 activity. The C-terminal domain of YB-1 is able to interact with PAR and control its synthesis, providing important insights into the regulation of PARP1 activity.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Cell Biology
Palmiro Poltronieri, Angela Celetti, Luca Palazzo
Summary: NAD(+) is crucial in post-translational modification of proteins and nucleic acids, with its synthesis, degradation, and transport playing a vital role in maintaining optimal NAD(+) levels. The functional connection between NAD(+-utilizing enzymes and mono(ADP-ribosyl)ating enzymes is linked to chronic diseases.
Article
Biochemistry & Molecular Biology
Liang Kong, Baomin Feng, Yan Yan, Chao Zhang, Jun Hyeok Kim, Lahong Xu, Johannes Gregor Matthias Rack, Ying Wang, Jyan-Chyun Jang, Ivan Ahel, Libo Shan, Ping He
Summary: This study reveals that the noncanonical ADP-ribosyltransferase SRO2 mediates the MARylation of zinc finger proteins SZF1 and SZF2, key regulators of immune gene expression. MARylation antagonizes polyubiquitination of SZF1, stabilizing the protein and maintaining protein homeostasis to mediate immune responses.
Article
Pharmacology & Pharmacy
Zhenzhen Li, Zhen Guo, Rui Lan, Sidong Cai, Zhirong Lin, Jingyan Li, Junjian Wang, Zhuoming Li, Peiqing Liu
Summary: BRD4 is crucial in the pathogenesis of cardiac hypertrophy by interacting with PARP1 to induce hypertrophic gene expression and transcription activation. Targeting the inhibition of PARP1-BRD4 interactions may have therapeutic potential for pathological cardiac hypertrophy.
ACTA PHARMACEUTICA SINICA B
(2021)
Article
Biochemistry & Molecular Biology
Zsolt Regdon, Agnieszka Robaszkiewicz, Katalin Kovacs, Zaneta Rygielska, Csaba Hegedus, Khaldon Bodoor, Eva Szabo, Laszlo Virag
FREE RADICAL BIOLOGY AND MEDICINE
(2019)
Article
Oncology
Maciej Sobczak, Andrew R. Pitt, Corinne M. Spickett, Agnieszka Robaszkiewicz
Article
Oncology
Maciej Sobczak, Julita Pietrzak, Tomasz Ploszaj, Agnieszka Robaszkiewicz
Article
Cell Biology
Abdennour Douida, Frank Batista, Agnieszka Robaszkiewicz, Pal Boto, Azzam Aladdin, Monika Szenykiv, Rita Czinege, Laszlo Virag, Krisztina Tar
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2020)
Review
Cell Biology
Maciej Sobczak, Marharyta Zyma, Agnieszka Robaszkiewicz
Article
Biochemistry & Molecular Biology
Abdennour Douida, Frank Batista, Pal Boto, Zsolt Regdon, Agnieszka Robaszkiewicz, Krisztina Tar
Summary: The study revealed that PA200 protein regulates gene expression in neuroblastoma cells by impacting metabolic processes such as glycolysis and mitochondrial function at the transcriptional level. Depletion of PA200 led to metabolic shifts towards glycolysis from oxidative phosphorylation, along with changes in mitochondrial morphology and proteolytic cleavage. These findings suggest a role for PA200 in modulating metabolic changes in response to ATP synthase inhibition in a cellular model.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Medicinal
Julita Pietrzak, Karolina Gronkowska, Agnieszka Robaszkiewicz
Summary: Secondary infections can lead to sepsis which may cause patient disability or death. Macrophages react to bacterial components by triggering an immune proinflammatory response, but the expression of proinflammatory cytokines significantly decreases after LPS stimulation. PARP1 plays a role in controlling macrophage immunoparalysis and may contribute to maintaining chromatin responsiveness during TLR activation.
Article
Oncology
Magdalena Strachowska, Karolina Gronkowska, Sylwia Michlewska, Agnieszka Robaszkiewicz
Summary: This study investigated the CBP/p300 bromodomain inhibitor I-CBP112, which can repress multidrug-resistance genes, enhance drug accumulation inside cells, and significantly potentiate drug effects. It was found that I-CBP112 decreased the expression of certain ABC transporters in breast, lung, and hepatic cancer cell lines, leading to increased sensitivity to a wide range of chemotherapeutics. This compound shows promise as a potent anti-multidrug-resistance agent in various cancer types.
Article
Cell Biology
Maciej Sobczak, Magdalena Strachowska, Karolina Gronkowska, Iwona Karwaciak, Lukasz Pulaski, Agnieszka Robaszkiewicz
Summary: The study shows that activation of toll-like receptors with LPS leads to a decline in the transcription of hydrogen peroxide decomposing enzyme-catalase, promoting polarization of human macrophages towards the pro-inflammatory phenotype. Inhibition of LSD1 activity in LPS-stimulated macrophages can negatively control expression of key pro-inflammatory markers.
Article
Oncology
Maciej Sobczak, Magdalena Strachowska, Karolina Gronkowska, Agnieszka Robaszkiewicz
Summary: In this research, the CoREST complex is identified as a key factor that controls the expression of ABC transporters in cisplatin-treated cancer cells, preventing multidrug resistance. The CoREST complex occupancy at gene promoters suppresses the EP300-dependent increase in ABCC transcription induced by cisplatin and gene overexpression in cisplatin-resistant phenotypes. The EP300-mediated activation of ABCC10 in response to cisplatin is only possible in the presence of p53.
Meeting Abstract
Oncology
A. Robaszkiewicz, K. Gronkowska
ANNALS OF ONCOLOGY
(2022)
Article
Medicine, Research & Experimental
Magdalena Strachowska, Karolina Gronkowska, Maciej Sobczak, Marika Grodzicka, Sylwia Michlewska, Kinga Kolacz, Tuhin Sarkar, Joanna Korszun, Maksim Ionov, Agnieszka Robaszkiewicz
Summary: This study tested the efficacy of I-CBP112, a CBP/EP300 bromodomain inhibitor, in overcoming drug resistance in breast cancer and lung cancer models. It was found that I-CBP112 significantly reduced the overexpression of ATP-binding cassette transporters, thereby increasing intracellular drug accumulation and cytotoxicity. The study also demonstrated that I-CBP112 polarized human macrophages into proinflammatory phenotypes. Importantly, I-CBP112 did not show toxicity to primary cell lines or enhance drug toxicity to blood-immune cells.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Meeting Abstract
Biochemistry & Molecular Biology
M. Strachowska, A. Robaszkiewicz
Meeting Abstract
Biochemistry & Molecular Biology
K. Gronkowska, T. Ploszaj, A. Robaszkiewicz
Article
Anthropology
Tomasz Ploszaj, Krystyna Jedrychowska-Danska, Alicja Zamerska, Magda Lewandowska, Jacek Bojarski, Wojciech Chudziak, Alicja Drozd-Lipinska, Agnieszka Robaszkiewicz, Henryk W. Witas
HOMO-JOURNAL OF COMPARATIVE HUMAN BIOLOGY
(2020)
Correction
Biochemistry & Molecular Biology
Siew Chin Chan, Chih-Wei Tung, Chia-Wei Lin, Yun-Shiuan Tung, Po-Min Wu, Pei-Hsun Cheng, Chuan-Mu Chen, Shang-Hsun Yang
FREE RADICAL BIOLOGY AND MEDICINE
(2024)
Article
Biochemistry & Molecular Biology
Suyuan Liu, Meiling Tan, Jiangxue Cai, Chenxuan Li, Miaoxin Yang, Xiaoxiao Sun, Bin He
Summary: This study reveals that the antibiotic doxycycline effectively inhibits NLRP3 inflammasome activation by targeting mitochondrial translation and mtDNA synthesis, offering potential for the treatment of NLRP3-related diseases.
FREE RADICAL BIOLOGY AND MEDICINE
(2024)
Article
Biochemistry & Molecular Biology
Hao Liu, Nana Li, Ge Kuang, Xia Gong, Ting Wang, Jun Hu, Hui Du, Minxuan Zhong, Jiashi Guo, Yao Xie, Yang Xiang, Shengwang Wu, Yiling Yuan, Xinru Yin, Jingyuan Wan, Ke Li
Summary: Protectin D1 (PTD1) improves hepatic steatosis, inflammation and fibrosis in a NASH mouse model by inhibiting the activation of TLR4 downstream signaling pathway, possibly through upregulation of IRAK-M expression, suggesting a potential new treatment for NASH.
FREE RADICAL BIOLOGY AND MEDICINE
(2024)
