Article
Genetics & Heredity
Heike Katrin Schreier, Rahel Stefanie Wiehe, Miria Ricchetti, Lisa Wiesmueller
Summary: This study identifies the role of polymerase zeta (POLZ) in maintaining mitochondrial DNA (mtDNA) under conditions of oxidative stress. POLZ knockdown leads to mtDNA accumulation and requires the activity of the base excision repair enzyme APE1. Additionally, POLZ is involved in the formation of mtDNA deletions independently of reactive oxygen species. These findings highlight the significance of POLZ in mtDNA turnover, particularly in the presence of oxidative stress.
Review
Cell Biology
Mansour Akbari, Hilde Loge Nilsen, Nicola Pietro Montaldo
Summary: Mitochondria are essential for cellular energy production and play a crucial role in organismal development and normal function. Understanding the mechanisms of mtDNA maintenance and transcription can provide insights into the pathogenesis of various diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Adib Behrouzi, Hanyu Xia, Eric L. Thompson, Mark R. Kelley, Jill C. Fehrenbacher
Summary: Cisplatin-induced neurotoxicity is a common complication of anti-cancer treatment. Inhibition of OGG1 and APE1 endonuclease activity exacerbates oxidative DNA damage and neurotoxicity in sensory neurons.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Medicinal
Yi Yuan, Dingqiang Fu, Yan Xu, Xuyang Wang, Xiongfei Deng, Shan Zhou, Feng Du, Xin Cui, Yun Deng, Zhuo Tang
Summary: This study describes a novel Pt(IV) prodrug, AP1, that targets the BER protein APE1 and induces DNA damage response and apoptosis in cancer cells. AP1 shows strong inhibitory effects on malignant cells, including cisplatin-resistant cells, with minimal toxicity to normal cells. It can also directly inhibit the activity of APE1 and interrupt miRNA processing, leading to the upregulation of the tumor suppressor PTEN.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Yong Woong Jun, Eddy Albarran, David L. Wilson, Jun Ding, Eric T. Kool
Summary: Mitochondrial function declines with aging and neurodegeneration, largely due to accumulated mutations in mtDNA from deficient DNA repair. A fluorescent probe, UBER, was used to visualize mitochondrial BER activity, showing increased repair under oxidative stress and time delay between stress onset and BER initiation. Cells lacking MTH1 exhibited elevated demand for BER activity during extended stress, while absence of OGG1 limited glycosylation capacity.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Biochemistry & Molecular Biology
Zhouyiyuan Xue, Bruce Demple
Summary: Apurinic/apyrimidinic endonuclease 1/redox effector-1 (Ape1/Ref-1) is an important enzyme in mammalian cells that plays a key role in DNA repair and transcription factor activation. Recent studies have shown that APEX1 knockout cell lines are more sensitive to the inhibitors Compound 3 and APX2009, suggesting off-target effects of these inhibitors.
Article
Biochemistry & Molecular Biology
Mansoor Hussain, Xixia Chu, Burcin Duan Sahbaz, Samuel Gray, Komal Pekhale, Jae-Hyeon Park, Deborah L. Croteau, Vilhelm A. Bohr
Summary: Aging is associated with decreased DNA repair efficiency and increased inflammation, contributing to age-related diseases. Mitochondrial OGG1 plays a role in DNA repair and improved mitochondrial function. Overexpression of mitochondria-targeted OGG1 can reverse aging-associated inflammation and improve functions in mice. The release of mtDNA into the cytoplasm is regulated by mtOGG1, which controls age-associated inflammation.
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Article
Agronomy
Shiwen Liu, Shuhua Zhu, Guangqin Jing
Summary: Excessive reactive oxygen species (ROS) during storage can lead to oxidative damage to mitochondrial DNA (mtDNA) in peaches, exacerbating fruit damage. Nitric oxide (NO) has been found to help maintain the storage quality of peaches. This study investigates the effects of NO on mtDNA damage and repair, focusing on the base excision repair (BER) pathway. The results show that NO reduces ROS levels and oxidative damage markers in mitochondria, increases mtDNA copy number, and enhances the BER pathway, leading to improved repair efficiency and maintenance of mtDNA integrity.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Cynthia J. Burrows, Shereen A. Howpay Manage, Aaron M. Fleming, Hsiao-Nung Chen
Summary: This study quantitatively investigated cysteine oxidation to sulfenic acid in APE1 and how this modified form affects APE1's binding to the NEIL3 gene promoter G4; the results suggest that cysteine oxidation can enhance G4 binding but reduce endonuclease activity in APE1, potentially triggering a switch from base excision repair to transcriptional modulation.
ACS CHEMICAL BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Inga R. Grin, Daria V. Petrova, Anton V. Endutkin, Chunquan Ma, Bing Yu, Haiying Li, Dmitry O. Zharkov
Summary: Base excision DNA repair is an important pathway in safeguarding the genome of plants, and understanding their repair mechanisms is crucial for developing new crop varieties.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Xiaoying Wei, Zhishuo Wang, Caroline Hinson, Kun Yang
Summary: The study found that human tyrosyl-DNA phosphodiesterase 1 (TDP1), AP endonuclease 1 (APE1), and three-prime repair exonuclease 1 (TREX1) can repair 3'-PUA-protein crosslinks, providing new repair pathways for maintaining genome integrity.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Shereen A. Howpay Manage, Aaron M. Fleming, Hsiao-Nung Chen, Cynthia J. Burrows
Summary: This study provides a quantitative survey of cysteine oxidation to sulfenic acid in APE1 and demonstrates how this modification affects APE1's function towards the NEIL3 gene promoter G4. The research suggests that cysteine oxidation to sulfenic acid enhances APE1's affinity for G4 during oxidative stress, but attenuates its endonuclease activity.
ACS CHEMICAL BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Jiahui Zhu, Lexin Liu, Xiaodi Ma, Xinyu Cao, Yu Chen, Xiangping Qu, Ming Ji, Huijun Liu, Chi Liu, Xiaoqun Qin, Yang Xiang
Summary: The mortality rate of idiopathic pulmonary fibrosis (IPF) increases yearly due to ineffective treatment. Oxidative stress, especially DNA stimulation, plays a crucial role in pulmonary fibrosis. DNA damage is important in the initiation of IPF and DNA repair systems targeting damage are crucial for lung cell survival. However, existing research has not focused on the role of DNA damage and repair pathways in IPF.
Review
Biochemistry & Molecular Biology
Weiran Zhang, Ranwei Zhong, Xiangping Qu, Yang Xiang, Ming Ji
Summary: Excess reactive oxygen species (ROS) can lead to oxidative stress by disrupting the balance between oxidation and anti-oxidation. The main product of ROS-induced base damage is 8-hydroxyguanine (8-oxoG), which can cause mutations if not promptly removed during DNA replication. The OGG1-mediated oxidative damage repair pathway plays an important role in preventing dysfunction caused by oxidative stress.
Article
Microbiology
Filipe Rollo, Guilherme D. Martins, Andre G. Gouveia, Solenne Ithurbide, Pascale Servant, Celia V. Romao, Elin Moe
Summary: This study demonstrates the importance of three Endonuclease III-like enzymes encoded by genes in Deinococcus radiodurans. While DrEndoIII2 is the main enzyme in this bacterium, DrEndoIII1 and 3 show unusual and undetectable activity, respectively. The knockout mutants of these genes were more resistant to oxidative stress induced by methyl viologen, indicating the crucial role of all three EndoIII homologs for cell survival. The upregulation of EndoIII homologs gene expression in response to oxidative stress may compensate for the loss of one gene.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Endocrinology & Metabolism
Song Han, Ella W. Englander, Guillermo A. Gomez, Cristiana Rastellini, Thomas Quertermous, Ramendra K. Kundu, George H. Greeley
Article
Biochemistry & Molecular Biology
Ming Zhuo, Murat F. Gorgun, Ella W. Englander
FREE RADICAL BIOLOGY AND MEDICINE
(2016)
Article
Neurosciences
Murat F. Gorgun, Ming Zhuo, Ella W. Englander
MOLECULAR NEUROBIOLOGY
(2017)
Article
Toxicology
Murat F. Gorgun, Ming Zhuo, IbDanelo Cortez, Kelly T. Dineley, Ella W. Englander
INHALATION TOXICOLOGY
(2017)
Article
Neurosciences
Ming Zhuo, Murat F. Gorgun, Ella W. Englander
MOLECULAR NEUROBIOLOGY
(2018)
Article
Gastroenterology & Hepatology
Song Han, Ella W. Englander, Guillermo A. Gomez, George H. Greeley
Article
Biochemistry & Molecular Biology
Ming Zhuo, Murat F. Gorgun, Ella W. Englander
FREE RADICAL BIOLOGY AND MEDICINE
(2018)
Article
Gastroenterology & Hepatology
Song Han, Ella W. Englander, Guillermo A. Gomez, Judith F. Aronson, Cristiana Rastellini, R. P. Garofalo, Deepthi Kolli, Thomas Quertermous, Ramendra Kundu, George H. Greeley
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
(2013)
Article
Genetics & Heredity
Ella W. Englander
Article
Toxicology
Falih Murat Gorgun, Ming Zhuo, Shilpee Singh, Ella W. Englander
INHALATION TOXICOLOGY
(2014)
Article
Biochemistry & Molecular Biology
Prachi Bajpai, Michelle C. Sangar, Shilpee Singh, Weigang Tang, Seema Bansal, Goutam Chowdhury, Qian Cheng, Ji-Kang Fang, Martha V. Martin, F. Peter Guengerich, Narayan G. Avadhani
JOURNAL OF BIOLOGICAL CHEMISTRY
(2013)
Article
Biochemistry & Molecular Biology
Shilpee Singh, Ming Zhuo, Falih M. Gorgun, Ella W. Englander
NITRIC OXIDE-BIOLOGY AND CHEMISTRY
(2013)
Article
Biochemistry & Molecular Biology
Murat F. Gorgun, Ming Zhuo, Kelly T. Dineley, Ella W. Englander
NEUROCHEMICAL RESEARCH
(2019)
Article
Biochemistry & Molecular Biology
Ming Zhuo, Falih M. Gorgun, Douglas S. Tyler, Ella W. Englander
Correction
Biochemistry & Molecular Biology
Siew Chin Chan, Chih-Wei Tung, Chia-Wei Lin, Yun-Shiuan Tung, Po-Min Wu, Pei-Hsun Cheng, Chuan-Mu Chen, Shang-Hsun Yang
FREE RADICAL BIOLOGY AND MEDICINE
(2024)
Article
Biochemistry & Molecular Biology
Suyuan Liu, Meiling Tan, Jiangxue Cai, Chenxuan Li, Miaoxin Yang, Xiaoxiao Sun, Bin He
Summary: This study reveals that the antibiotic doxycycline effectively inhibits NLRP3 inflammasome activation by targeting mitochondrial translation and mtDNA synthesis, offering potential for the treatment of NLRP3-related diseases.
FREE RADICAL BIOLOGY AND MEDICINE
(2024)
Article
Biochemistry & Molecular Biology
Hao Liu, Nana Li, Ge Kuang, Xia Gong, Ting Wang, Jun Hu, Hui Du, Minxuan Zhong, Jiashi Guo, Yao Xie, Yang Xiang, Shengwang Wu, Yiling Yuan, Xinru Yin, Jingyuan Wan, Ke Li
Summary: Protectin D1 (PTD1) improves hepatic steatosis, inflammation and fibrosis in a NASH mouse model by inhibiting the activation of TLR4 downstream signaling pathway, possibly through upregulation of IRAK-M expression, suggesting a potential new treatment for NASH.
FREE RADICAL BIOLOGY AND MEDICINE
(2024)