Review
Pharmacology & Pharmacy
Song Yang, Jun Zhou, Dengwen Li
Summary: The retinal pigment epithelium is crucial for visual functions, but damage to its structure and function can lead to various retinopathies with no cure currently available. Understanding the development, function, and pathobiology of the retinal pigment epithelium is essential for preventing and treating retinopathies. This review discusses potential strategies for treatment and provides new perspectives for future research in this field.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Genetics & Heredity
Xiuxiu Jin, Jingyang Liu, Weiping Wang, Jiangfeng Li, Guangming Liu, Ruiqi Qiu, Mingzhu Yang, Meng Liu, Lin Yang, Xiaofeng Du, Bo Lei
Summary: This study analyzed the molecular changes of retinal pigment epithelium (RPE) cells during aging through quantitative proteomic analysis. The findings suggest that protein ubiquitination related to RNF123 and RNF149 plays a crucial role in protecting RPE cells from oxidative damage.
GENOMICS PROTEOMICS & BIOINFORMATICS
(2022)
Article
Ophthalmology
Kristine A. Tsantilas, Whitney M. Cleghorn, Celia M. Bisbach, Jeremy A. Whitson, Daniel T. Hass, Brian M. Robbings, Martin Sadilek, Jonathan D. Linton, Austin M. Rountree, Ana P. Valencia, Mariya T. Sweetwyne, Matthew D. Campbell, Huiliang Zhang, Connor S. R. Jankowski, Ian R. Sweet, David J. Marcinek, Peter S. Rabinovitch, James B. Hurley
Summary: The study revealed that aged mice had reduced retinal responses to light stimuli, with relatively stable metabolic flux and ATP levels in retinal tissues. However, there was a decrease in glutamine utilization in the Krebs Cycle in eyecups while other metabolic parameters remained constant. This suggests that external factors may play a role in influencing ocular metabolism in vivo.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Jeremie Canonica, Min Zhao, Tatiana Favez, Emmanuelle Gelize, Laurent Jonet, Laura Kowalczuk, Justine Guegan, Damien Le Menuet, Say Viengchareun, Marc Lombes, Eric Pussard, Yvan Arsenijevic, Francine Behar-Cohen
Summary: Glucocorticoids are commonly used drugs to treat retinal diseases, but the transcriptional regulations induced by GR and MR activation in RPE cells are not well understood. The study found that in the human eye, cortisol is the main ligand for GR and MR, and genes regulated by aldosterone and cortisol + RU-486 can mimic an imbalance toward MR activation. This activation in RPE cells may contribute to retinal pathology.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Editorial Material
Endocrinology & Metabolism
Zhongjie Fu, Lois E. H. Smith
Summary: Pathologic angiogenesis in eye diseases leading to blindness can be attributed to cell senescence, as demonstrated by the bioinformatics study conducted by Crespo-Garcia, Tsuruda, and Dejda. Their findings were validated in human and mouse retina models, where clearance of senescent cells effectively suppressed neovessel growth.
TRENDS IN ENDOCRINOLOGY AND METABOLISM
(2021)
Article
Biochemistry & Molecular Biology
Kei Takahashi, Shinsuke Nakamura, Masamitsu Shimazawa, Hideaki Hara
Summary: This study characterized the retinal phenotype in mature PGRN knockout mice, showing reduced a-wave amplitude and outer nuclear thickness in Grn(-/-) mice compared to wild-type mice. Increased accumulation of retinal microglial cells on the RPE apical layer and changes in lysosomal protein expression were observed in Grn(-/-) mice, indicating a potential modulation of microglial dynamics in PGRN deficiency.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Jongmin Kim, Jae Yon Won
Summary: As the world ages, the number of age-related diseases, particularly those related to retinal pigment epithelium (RPE) degeneration, has increased. Photobiomodulation (PBM), a non-invasive treatment using red and near-infrared light, has shown potential as a new treatment for ocular diseases. Recent studies have demonstrated its antioxidant effect, making it a promising approach to cure RPE degeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Ignacio Ramirez-Pardo, Beatriz Villarejo-Zori, Juan Ignacio Jimenez-Loygorri, Elena Sierra-Filardi, Sandra Alonso-Gil, Guillermo Marino, Pedro de la Villa, Patrick S. Fitze, Jose Manuel Fuentes, Ramon Garcia-Escudero, Deborah A. Ferrington, Raquel Gomez-Sintes, Patricia Boya
Summary: Macroautophagy/autophagy is a key process in maintaining cellular homeostasis, and a decline in retinal autophagy has been associated with photoreceptor degeneration. A study on mice with a haploinsufficiency for Ambra1, a pro-autophagic gene, has shown an accelerated aging phenotype in the retina, with protein aggregation, metabolic underperformance, and premature vision loss. These findings emphasize the importance of autophagy in supporting RPE-retinal metabolism and protecting against stress and physiological aging.
Review
Cell Biology
Jie Cheng, Casey J. Keuthan, Noriko Esumi
Summary: SIRT6 is a versatile protein that plays a key role in maintaining cellular and organismal homeostasis. It has various functions in gene silencing, metabolism, DNA repair, antioxidant defense, inflammation, aging and longevity, early development, stress response, macrophage polarization, and mitochondrial homeostasis. Recent studies have revealed its critical functions in the retina and RPE, such as regulating energy metabolism, protecting against oxidative stress, and participating in early neurodegenerative events in diabetic retinopathy.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Christopher DeVera, Jendayi Dixon, Micah A. Chrenek, Kenkichi Baba, Yun Z. Le, P. Michael Iuvone, Gianluca Tosini
Summary: The study found that the circadian clock in the retinal pigment epithelium (RPE) controls the daily peak of phagocytic activity. However, the absence of a clock in the RPE does not result in deterioration of photoreceptors or the RPE during aging.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Antonio E. Paniagua, Alicia Segurado, Jorge F. Dolon, Julian Esteve-Rudd, Almudena Velasco, David S. Williams, Concepcion Lillo
Summary: The study reveals that CRB2 plays a crucial role in the polarization process of human RPE cells, contributing to tight junction maintenance and cell cycle arrest. Knockdown of CRB2 in RPE cells affects the distribution of apical polarity proteins and disrupts retinal homeostasis by causing invasion of activated microglial cells into the subretinal space. Overall, CRB2 is identified as a key protein for the development and maintenance of a polarized epithelium.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Pharmacology & Pharmacy
R. Scott Duncan, Daniel T. Hurtado, Conner W. Hall, Peter Koulen
Summary: The purpose of this study was to determine the efficacy and mechanism of action of different vitamin E components in protecting RPE cells from oxidative damage. The study found that α T requires protein synthesis and transport/signaling for optimal cytoprotection, while γ T and δ T do not.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Cell & Tissue Engineering
Helena Isla-Magrane, Anna Veiga, Jose Garcia-Arumi, Anna Duarri
Summary: A two-step method was established to obtain self-organized multizone ocular progenitor cells (mzOPCs) from 2D hiPSC cultures within three weeks, which were then grown in suspension to create 3D multiocular organoids. These organoids exhibited correct layering of retinal regions, resembled transparent ocular-surface epithelium in the corneal regions, and contained interactions between different cell types and regions of the eye system. This approach demonstrated the advantages of the combined 2D-3D construction tissue model in creating a more ocular native-like cellular environment for modeling eye development and disease.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Microbiology
Liam M. Ashander, Amanda L. Lumsden, Abby C. Dawson, Yuefang Ma, Lisia B. Ferreira, Genevieve F. Oliver, Binoy Appukuttan, Jillian M. Carr, Justine R. Smith
Summary: The prevalence of dengue retinopathy varies across epidemics, and is linked to the circulation of dengue virus serotype 1 (DENV-1). This study investigated the infectivity, molecular response, and barrier function of epithelial cells infected with DENV strains from different outbreaks in Singapore. The results showed that DENV-1 strains from the 2005 epidemic, when retinopathy was prevalent, had higher viral RNA loads and up-regulated interferon transcripts compared to DENV-1 strains from the 2007 epidemic when retinopathy was not observed. The strain of DENV may play a role in the development of retinal pathology.
Article
Biochemistry & Molecular Biology
Shuyan Zhou, Jacob Hum, Kaan Taskintuna, Stephanie Olaya, Jeremy Steinman, Junfeng Ma, Nady Golestaneh
Summary: It has been found that the Klotho gene can suppress aging when overexpressed in mice and accelerate aging when deleted. This study demonstrates that Klotho regulates cell viability, mitochondrial gene expression and activity, as well as RPE/retinal resistance to oxidative stress. These findings highlight the important role of Klotho in aging and retinal health.
Article
Immunology
Xia Dong, Weiju Wu, Liang Ma, Chengfei Liu, Mohajeet B. Bhuckory, Liping Wang, Emeline F. Nandrot, Heping Xu, Ke Li, Yizhi Liu, Wuding Zhou
JOURNAL OF INNATE IMMUNITY
(2017)
Article
Cell & Tissue Engineering
Sacha Reichman, Amelie Slembrouck, Giuliana Gagliardi, Antoine Chaffiol, Angelique Terray, Celine Nanteau, Anais Potey, Morgane Belle, Oriane Rabesandratana, Jens Duebel, Gael Orieux, Emeline F. Nandrot, Jose-Alain Sahel, Olivier Goureau
Article
Immunology
Sara Touhami, Fanny Beguier, Sebastien Augustin, Hugo Charles-Messance, Lucile Vignaud, Emeline F. Nandrot, Sacha Reichman, Valerie Forster, Thibaud Mathis, Jose-Alain Sahel, Bahram Bodaghi, Xavier Guillonneau, Florian Sennlaub
JOURNAL OF NEUROINFLAMMATION
(2018)
Article
Biotechnology & Applied Microbiology
Christo Kole, Laurence Klipfel, Ying Yang, Vanessa Ferracane, Frederic Blond, Sacha Reichman, Geraldine Millet-Puel, Emmanuelle Clerin, Najate Ait-Ali, Delphine Pagan, Hawa Camara, Marie-Noelle Delyfer, Emeline F. Nandrot, Jose-Alain Sahel, Olivier Goureau, Thierry Leveillard
Article
Pathology
Michael H. Farkas, Deborah S. Lew, Maria E. Sousa, Kinga Bujakowska, Jonathan Chatagnon, Shomi S. Bhattacharya, Eric A. Pierce, Emeline F. Nandrot
AMERICAN JOURNAL OF PATHOLOGY
(2014)
Article
Biochemistry & Molecular Biology
Ah-Lai Law, Celia Parinot, Jonathan Chatagnon, Basile Gravez, Jose-Alain Sahel, Shomi S. Bhattacharya, Emeline F. Nandrot
JOURNAL OF BIOLOGICAL CHEMISTRY
(2015)
Article
Multidisciplinary Sciences
Sacha Reichman, Angelique Terray, Amelie Slembrouck, Celine Nanteau, Gael Orieux, Walter Habeler, Emeline F. Nandrot, Jose-Alain Sahel, Christelle Monville, Olivier Goureau
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2014)
Article
Multidisciplinary Sciences
Alvaro Plaza Reyes, Sandra Petrus-Reurer, Sara Padrell Sanchez, Pankaj Kumar, Iyadh Douagi, Hammurabi Bartuma, Monica Aronsson, Sofie Westman, Emma Lardner, Helder Andre, Anna Falk, Emeline F. Nandrot, Anders Kvanta, Fredrik Lanner
NATURE COMMUNICATIONS
(2020)
Article
Cell & Tissue Engineering
Sandra Petrus-Reurer, Pankaj Kumar, Sara Padrell Sanchez, Monica Aronsson, Helder Andre, Hammurabi Bartuma, Alvaro Plaza Reyes, Emeline F. Nandrot, Anders Kvanta, Fredrik Lanner
STEM CELLS TRANSLATIONAL MEDICINE
(2020)
Article
Cell Biology
Laurence Klipfel, Marie Cordonnier, Lea Thiebault, Emmanuelle Clerin, Frederic Blond, Geraldine Millet-Puel, Saddek Mohand-Said, Olivier Goureau, Jose-Alain Sahel, Emeline F. Nandrot, Thierry Leveillard
Summary: Age-related macular degeneration (AMD) is a blinding disease with limited treatment options, and researchers have discovered a rare allele in the SLC16A8 gene that may be associated with vision impairments through genetic studies.
Article
Biochemistry & Molecular Biology
Francesca Mazzoni, Ying Dun, Jade A. Vargas, Emeline F. Nandrot, Silvia C. Finnemann
Summary: A lack of methionine sulfoxide reductase A in mice leads to retinal protein oxidation, decreased mitochondrial function, and impaired phagocytic ability. Despite normal expression and localization of cone opsins, cone photoreceptors in these mice show reduced light responses, decreased lectin-labeled cone sheaths, and reduced cone arrestin. These cone defects can be completely prevented by dark rearing.
Article
Multidisciplinary Sciences
Pierre-Henry Gabrielle, Laurence Faivre, Isabelle Audo, Xavier Zanlonghi, Helene Dollfus, Alberta A. H. J. Thiadens, Christina Zeitz, Grazia M. S. Mancini, Yaumara Perdomo, Saddek Mohand-Said, Eleonore Lize, Vincent Lhussiez, Emeline F. Nandrot, Niyazi Acar, Catherine Creuzot-Garcher, Jose-Alain Sahel, Muhammad Ansar, Christel Thauvin-Robinet, Laurence Duplomb, Romain Da Costa
Summary: Cohen syndrome patients have a high prevalence of cystoid maculopathy (CM), with carbonic anhydrase inhibitors (CAI) treatment potentially not sufficient, and CM may resolve spontaneously during adulthood as photoreceptor dystrophy progresses. The progression and stability of CM vary among patients of different age groups.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Quentin Rieu, Antoine Bougouin, Yvrick Zagar, Jonathan Chatagnon, Abdallah Hamieh, Julie Enderlin, Thierry Huby, Emeline F. Nandrot
Summary: The scavenger receptor family members, including CD36 and SR-B2/LIMP-2, play a direct regulatory role in the phagocytic dynamics of photoreceptor outer segments (POS) in the retina. Other receptors such as MARCO may participate in the clearance of POS by retinal pigment epithelial (RPE) cells either as co-receptors or through an indirect process.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell & Tissue Engineering
Amelie Rodrigues, Amelie Slembrouck-Brec, Celine Nanteau, Angelique Terray, Yelyzaveta Tymoshenko, Yvrick Zagar, Sacha Reichman, Zhouhuan Xi, Jose-Alain Sahel, Stephane Fouquet, Gael Orieux, Emeline F. Nandrot, Leah C. Byrne, Isabelle Audo, Jerome E. Roger, Olivier Goureau
Summary: Mutations in the PRPF31 gene, a common cause of Retinitis Pigmentosa, lead to specific changes in retinal cells. Using retinal organoids and RPE cells derived from human iPSCs, we observed morphological and functional changes in cells carrying PRPF31 mutations, and replicated the human RP phenotype in retinal organoids.
NPJ REGENERATIVE MEDICINE
(2022)
Article
Ophthalmology
Vincent Lhussiez, Elisabeth Dubus, Quenol Cesar, Niyazi Acar, Emeline F. Nandrot, Manuel Simonutti, Isabelle Audo, Eleonore Lize, Sylvie Nguyen, Audrey Geissler, Andre Bouchot, Muhammad Ansar, Serge Picaud, Christel Thauvin-Robinet, Laurence Olivier-Faivre, Laurence Duplomb, Romain Da Costa
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2020)
Correction
Biochemistry & Molecular Biology
Siew Chin Chan, Chih-Wei Tung, Chia-Wei Lin, Yun-Shiuan Tung, Po-Min Wu, Pei-Hsun Cheng, Chuan-Mu Chen, Shang-Hsun Yang
FREE RADICAL BIOLOGY AND MEDICINE
(2024)
Article
Biochemistry & Molecular Biology
Suyuan Liu, Meiling Tan, Jiangxue Cai, Chenxuan Li, Miaoxin Yang, Xiaoxiao Sun, Bin He
Summary: This study reveals that the antibiotic doxycycline effectively inhibits NLRP3 inflammasome activation by targeting mitochondrial translation and mtDNA synthesis, offering potential for the treatment of NLRP3-related diseases.
FREE RADICAL BIOLOGY AND MEDICINE
(2024)
Article
Biochemistry & Molecular Biology
Hao Liu, Nana Li, Ge Kuang, Xia Gong, Ting Wang, Jun Hu, Hui Du, Minxuan Zhong, Jiashi Guo, Yao Xie, Yang Xiang, Shengwang Wu, Yiling Yuan, Xinru Yin, Jingyuan Wan, Ke Li
Summary: Protectin D1 (PTD1) improves hepatic steatosis, inflammation and fibrosis in a NASH mouse model by inhibiting the activation of TLR4 downstream signaling pathway, possibly through upregulation of IRAK-M expression, suggesting a potential new treatment for NASH.
FREE RADICAL BIOLOGY AND MEDICINE
(2024)