Article
Immunology
Dipasree Hajra, Abhilash Vijay Nair, Atish Roy Chowdhury, Soumyadeep Mukherjee, Ritika Chatterjee, Dipshikha Chakravortty
Summary: This study reveals a novel role of U32 peptidase YdcP in Salmonella Typhimurium by protecting the bacteria from oxidative stress. The knockout of YdcP leads to attenuated intracellular proliferation of Salmonella and increased susceptibility to in vitro oxidative stress.
MICROBIAL PATHOGENESIS
(2022)
Article
Chemistry, Medicinal
Jie Zang, Felix Peters, Yves Cambet, Eugenia Cifuentes-Pagano, Munira Mohamed Shishay Hissabu, Christopher M. Dustin, Lars Henrik Svensson, Martin Mariboe Olesen, Mathias Feldt Lomholt Poulsen, Stig Jacobsen, Pernille Sonderby Tuelung, Dilip Narayanan, Annette Eva Langkilde, Michael Gajhede, Patrick J. Pagano, Vincent Jaquet, Frederik Vilhardt, Anders Bach
Summary: In this study, we optimized and explored bivalent small-molecule drugs targeting the NOX2 subunit p47phox and identified compounds with high binding affinities and cellular activities, demonstrating the potential of p47phox as an effective small-molecule target. This has important implications for the research and development of therapeutic drugs for related diseases.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Cell Biology
Daniel Simoes de Jesus, Paula Bargi-Souza, Vinicius Cruzat, Vijay Yechoor, Angelo Rafael Carpinelli, Rodrigo Antonio Peliciari-Garcia
Summary: The pancreatic beta cells circadian clock and NADPH oxidase (NOX) family play important roles in glucose metabolism. Absence of BMAL1 may trigger an increase in NOX2-derived reactive oxygen species (ROS) in pancreatic beta cells, leading to inhibition of insulin secretion.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2022)
Article
Multidisciplinary Sciences
Guzal Khayrullina, Sara Bermudez, Deanna Hopkins, Young Yauger, Kimberly R. R. Byrnes
Summary: NOX2 knockout improves recovery after spinal cord injury, while acute inhibition of NOX4 fails to enhance long-term recovery. Both NOX2 and NOX4 reduce ROS production and oxidative stress markers, and NOX2 knockout shows a shift in microglial activation towards a more neuroprotective state.
Article
Biochemistry & Molecular Biology
Xavier Serfaty, Pauline Lefrancois, Chantal Houee-Levin, Stephane Arbault, Laura Baciou, Tania Bizouarn
Summary: The study reveals that monitoring NADPH oxidation instead of cyt c reduction provides a more accurate measurement of Nox2 activity, indicating a potential underestimation of enzyme activity using the traditional method.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2021)
Article
Biochemistry & Molecular Biology
Jonathan R. Erlich, Eunice E. To, Raymond Luong, Felicia Liong, Stella Liong, Osezua Oseghale, Mark A. Miles, Steven Bozinovski, Robert D. Brooks, Ross Vlahos, Stanley Chan, John J. O'Leary, Doug A. Brooks, Stavros Selemidis
Summary: The study reveals that macrophages undergo a metabolic switch from oxidative phosphorylation to glycolysis when exposed to gram-negative bacterial lipopolysaccharide (LPS). This switch modulates antibacterial host defence mechanisms by increasing the activity of NOX2 enzyme and promoting the expression of type I IFN-beta. Both glycolysis and the pentose phosphate pathway play important roles in promoting LPS-induced inflammation in macrophages.
Article
Microbiology
Hasnaa Maksouri, Dounia Darif, Jerome Estaquier, Myriam Riyad, Christophe Desterke, Meryem Lemrani, Pham My-Chan Dang, Khadija Akarid
Summary: This study evaluated the impact of Moroccan strains of L. major and L. tropica on PMNs. The results showed that L. tropica inhibited O-2(-) production, while L. major did not. Additionally, Leishmania soluble antigens from both species inhibited O-2(-) induction.
Review
Cell Biology
Marie-Helene Paclet, Salome Laurans, Sophie Dupre-Crochet
Summary: This article reviews the multiple roles of NOX2 in neutrophil functions, with a focus on the description of its components and their assembly mechanisms. It also explains the role of energy metabolism and phosphoinositides in regulating NADPH oxidase activity.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Dezhen Tu, Ravikanth Velagapudi, Yun Gao, Jau-Shyong Hong, Hui Zhou, Hui-Ming Gao
Summary: This study investigates the expression patterns, regulatory mechanisms, and pathological roles of neuronal NADPH oxidase in inflammation-associated neurodegeneration. The results show that NOX2 is persistently upregulated in neurons during chronic neuroinflammation and is associated with oxidative stress and neurodegeneration. Inhibition of neuronal NOX2 can block the degenerative effects of inflammatory mediators and prevent neurodegeneration.
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Carla Ijurko, Marta Romo-Gonzalez, Clara Garcia-Calvo, Jose Luis Sardina, Carmen Sanchez-Bernal, Jesus Sanchez-Yaguee, Benedicte Elena-Herrmann, Joran Villaret, Catherine Garrel, Julie Mondet, Pascal Mossuz, Angel Hernandez-Hernandez
Summary: Acute myeloid leukaemia (AML) is a highly heterogeneous disease, and the lack of NOX2 protein affects the metabolism control of AML cells. A panel of differentially expressed metabolic genes has been discovered, which has predictive value for AML prognosis and survival.
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Article
Cell Biology
Edna Bechor, Anat Zahavi, Yevgeny Berdichevsky, Edgar Pick
Summary: This study demonstrates the important role of p67(phox) in the assembly/activation of NADPH oxidase through direct interaction with Nox2. Additionally, it shows that Rac-GTP acts as a carrier for p67(phox) to the membrane and induces a conformational change that enhances its affinity for Nox2, unmasking a previously hidden binding site.
JOURNAL OF LEUKOCYTE BIOLOGY
(2021)
Article
Immunology
Chih-Fen Hu, San-Pin Wu, Gu-Jiun Lin, Chi-Chang Shieh, Chih-Sin Hsu, Jing-Wun Chen, Shih-Heng Chen, Jau-Shyong Hong, Shyi-Jou Chen
Summary: The study showed that Nox2 deficiency attenuates neural damage induced by EAE, reduces disease severity, pathogenic immune cells infiltration, demyelination, and oxidative stress in the CNS. It also revealed that microglial Nox2 plays a regulatory role in multiple pathways important for MS/EAE pathogenesis, suggesting that Nox2 inhibition may represent an important therapeutic target for MS.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Endocrinology & Metabolism
Alessio Alfieri, Juraj Koudelka, Mosi Li, Sanny Scheffer, Jessica Duncombe, Andrea Caporali, Rajesh N. Kalaria, Colin Smith, Ajay M. Shah, Karen Horsburgh
Summary: Chronic microvascular inflammation and oxidative stress are important mechanisms underlying white matter disease and vascular cognitive impairment (VCI). Nox2 plays a critical role, and its genetic deletion can protect against white matter pathology and cognitive impairment. This study has clinical implications for the treatment of vascular diseases.
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
(2022)
Article
Immunology
Anthos Christofides, Xanthi-Lida Katopodi, Carol Cao, Dimitra Karagkouni, Konstantinos Aliazis, Sasitorn Yenyuwadee, Halil-Ibrahim Aksoylar, Rinku Pal, Mohamed A. A. Mahmoud, Laura Strauss, Natalia M. Tijaro-Ovalle, Louis Boon, John Asara, Ioannis S. Vlachos, Nikolaos Patsoukis, Vassiliki A. Boussiotis
Summary: Mice with targeted SHP-2 deletion in myeloid cells showed reduced tumor growth, similar to mice with targeted PD-1 deletion in myeloid cells. SHP-2 and PD-1-SHP-2 signaling suppress myeloid cell differentiation, resulting in suppressed antitumor immunity.
Article
Biochemistry & Molecular Biology
Carolina Prolo, Damian Estrada, Lucia Piacenza, Diego Benitez, Marcelo A. Comini, Rafael Radi, Maria Noel Alvarez
Summary: This study shows that the lack of superoxide radical produced by Nox2-deficient macrophages leads to increased susceptibility to T. cruzi infection in a murine model. Reconstitution of intraphagosomal superoxide radical formation in these macrophages restores the control of infection. The presence of Nox2-derived superoxide radical plays a crucial role in controlling early phase T. cruzi infection in a murine model of Chagas disease.
Article
Medicine, General & Internal
Ching-Long Lai, Chun-Chung Lu, Hui-Ching Lin, Yueh-Feng Sung, Yi-Pin Wu, Jau-Shyong Hong, Giia-Sheun Peng
JOURNAL OF THE FORMOSAN MEDICAL ASSOCIATION
(2019)
Article
Neurosciences
Sheng Song, Lulu Jiang, Esteban A. Oyarzabal, Belinda Wilson, Zibo Li, Yen-Yu Ian Shih, Qingshan Wang, Jau-Shyong Hong
MOLECULAR NEUROBIOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Ran Zhou, Shih-Heng Chen, Guorong Li, Hui-Ling Chen, YuXin Liu, Hung-Ming Wu, Yubao Wang, Jing Feng, Jau-Shyong Hong
CHEMICO-BIOLOGICAL INTERACTIONS
(2019)
Article
Substance Abuse
Ru-Band Lu, Tzu-Yun Wang, Sheng-Yu Lee, Shiou-Lan Chen, Yun-Hsuan Chang, Po See Chen, Shih-Hsien Lin, Chun-Hsien Chu, San-Yuan Huang, Nian-Sheng Tzeng, I. Hui Lee, Kao Chin Chen, Yen Kuang Yang, Ping Chen, Shih-Heng Chen, Jau-Shyong Hong
DRUG AND ALCOHOL DEPENDENCE
(2019)
Article
Neurosciences
Ru-Band Lu, Yun-Hsuan Chang, Sheng-Yu Lee, Tzu-Yun Wang, Shu-Li Cheng, Po-See Chen, Yen-Kuang Yang, Jau-Shyong Hong, Shiou-Lan Chen
CLINICAL PSYCHOPHARMACOLOGY AND NEUROSCIENCE
(2020)
Article
Biochemistry & Molecular Biology
Charles Romeo, Shih-Heng Chen, Eugenia Goulding, Lucas Van Gorder, Maura Schwartz, Mitzie Walker, Gregory Scott, Erica Scappini, Manas Ray, Negin P. Martin
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2020)
Article
Cell Biology
Sawako Shindo, Shih-Heng Chen, Saki Gotoh, Kosuke Yokobori, Hao Hu, Manas Ray, Rick Moore, Kiyoshi Nagata, Jennifer Martinez, Jau-Shyong Hong, Masahiko Negishi
CELL COMMUNICATION AND SIGNALING
(2020)
Article
Biochemical Research Methods
Shih-Heng Chen, Amy Papaneri, Mitzie Walker, Erica Scappini, Robert D. Keys, Negin P. Martin
JOURNAL OF VIROLOGICAL METHODS
(2020)
Article
Multidisciplinary Sciences
Alexander C. Y. Foo, Peter M. Thompson, Shih-Heng Chen, Ramesh Jadi, Brianna Lupo, Eugene F. DeRose, Simrat Arora, Victoria C. Placentra, Lakshmanane Premkumar, Lalith Perera, Lars C. Pedersen, Negin Martin, Geoffrey A. Mueller
Summary: The mosquito protein AEG12 has been shown to inhibit replication of enveloped viruses such as Dengue and Zika viruses by selectively delivering unsaturated fatty acids into lipid membranes, demonstrating its potential as a broad-spectrum therapeutic agent.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Immunology
Chih-Fen Hu, San-Pin Wu, Gu-Jiun Lin, Chi-Chang Shieh, Chih-Sin Hsu, Jing-Wun Chen, Shih-Heng Chen, Jau-Shyong Hong, Shyi-Jou Chen
Summary: The study showed that Nox2 deficiency attenuates neural damage induced by EAE, reduces disease severity, pathogenic immune cells infiltration, demyelination, and oxidative stress in the CNS. It also revealed that microglial Nox2 plays a regulatory role in multiple pathways important for MS/EAE pathogenesis, suggesting that Nox2 inhibition may represent an important therapeutic target for MS.
FRONTIERS IN IMMUNOLOGY
(2021)
Correction
Endocrinology & Metabolism
Tzu-Yun Wang, Sheng-Yu Lee, Ming-Chuan Hu, Shiou-Lan Chen, Yun-Hsuan Chang, Chun-Hsien Chu, Shih-Hsien Lin, Chia-Ling Li, Liang-Jen Wang, Po See Chen, Shih-Heng Chen, San-Yuan Huang, Nian-Sheng Tzeng, I. Hui Lee, Kao Chin Chen, Yen Kuang Yang, Jau-Shyong Hong, Ru-Band Lu
PSYCHONEUROENDOCRINOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Shih-Heng Chen, Shuangyu Han, Chih-Fen Hu, Ran Zhou, Yun Gao, Dezhen Tu, Huiming Gao, Jing Feng, Yubao Wang, Ru-Band Lu, Jau-Shyong Hong
Summary: Recent studies have found that improper resolution of acute neuroinflammation can lead to long-lasting low-grade chronic neuroinflammation and progressive neurodegeneration. This study aimed to identify the potential pathways mediating chronic neuroinflammation and resultant neurodegeneration following LPS stimulation. The results showed that TLR-4 activation, which is essential for acute neuroinflammation, is not sufficient for maintaining chronic neuroinflammation. Instead, the MAC1-NOX2-ERK1/2 signaling pathway plays a crucial role in the initiation and maintenance of low-grade chronic neuroinflammation. Continuous activation of ERK1/2 and NOX2 in microglia forms a vicious cycle, sustaining the low-grade neuroinflammation and driving neurodegeneration.
Article
Microbiology
Christopher McGee, Min Shi, John House, Anna Drude, Gladys Gonzalez, Negin Martin, Shih-Heng Chen, Heidi Rogers, Alex Njunge, Xiomara Hodge, Brittany Mosley, Margaret George, Ruhani Agrawal, Catherine Wild, Cynthia Smith, Audrey Brown, Lisa Barber, Stavros Garantziotis
Summary: The COVID-19 pandemic continues to impact societies and healthcare systems worldwide. Vaccination and social distancing behavior are positively correlated. Breakthrough infections are associated with low antibody titers.
MICROBIOLOGY SPECTRUM
(2022)
Article
Neurosciences
Ran Zhou, Shih-Heng Chen, Zhan Zhao, Dezhen Tu, Sheng Song, Yubao Wang, Qingshan Wang, Jing Feng, Jau-Shyong Hong
Summary: Recent studies have found increased expression of complements in neurodegenerative diseases like Alzheimer's and Parkinson's diseases. However, the mechanism and role of complements in neurodegeneration are unclear. This study showed that acute neuroinflammation increased the expression of complement C3, and sustained expression during chronic neuroinflammation required the release of damage-associated molecule patterns (DAMPs). The activation of microglial NADPH oxidase (NOX2) was found to be involved in the sustained expression of C3. The findings suggest a role of C3 in regulating chronic neuroinflammation and driving progressive neurodegeneration.
MOLECULAR NEUROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Kaoru Inoue, Hamed Bostan, MaKenna R. Browne, Owen F. Bevis, Carl D. Bortner, Steven A. Moore, Aaron A. Stence, Negin P. Martin, Shih-Heng Chen, Adam B. Burkholder, Jian-Liang Li, Natalie D. Shaw
Summary: SMCHD1 mutations cause congenital arhinia and FSHD2. Loss of SMCHD1 activity leads to DUX4 expression and cell death in placode cells derived from hESCs and iPSCs. Herpesvirus infection may amplify DUX4 expression in SMCHD1 KO cells, indicating an environmental disease modifier.
Correction
Biochemistry & Molecular Biology
Siew Chin Chan, Chih-Wei Tung, Chia-Wei Lin, Yun-Shiuan Tung, Po-Min Wu, Pei-Hsun Cheng, Chuan-Mu Chen, Shang-Hsun Yang
FREE RADICAL BIOLOGY AND MEDICINE
(2024)
Article
Biochemistry & Molecular Biology
Suyuan Liu, Meiling Tan, Jiangxue Cai, Chenxuan Li, Miaoxin Yang, Xiaoxiao Sun, Bin He
Summary: This study reveals that the antibiotic doxycycline effectively inhibits NLRP3 inflammasome activation by targeting mitochondrial translation and mtDNA synthesis, offering potential for the treatment of NLRP3-related diseases.
FREE RADICAL BIOLOGY AND MEDICINE
(2024)
Article
Biochemistry & Molecular Biology
Hao Liu, Nana Li, Ge Kuang, Xia Gong, Ting Wang, Jun Hu, Hui Du, Minxuan Zhong, Jiashi Guo, Yao Xie, Yang Xiang, Shengwang Wu, Yiling Yuan, Xinru Yin, Jingyuan Wan, Ke Li
Summary: Protectin D1 (PTD1) improves hepatic steatosis, inflammation and fibrosis in a NASH mouse model by inhibiting the activation of TLR4 downstream signaling pathway, possibly through upregulation of IRAK-M expression, suggesting a potential new treatment for NASH.
FREE RADICAL BIOLOGY AND MEDICINE
(2024)