期刊
FREE RADICAL BIOLOGY AND MEDICINE
卷 49, 期 4, 页码 674-681出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.freeradbiomed.2010.05.025
关键词
Ovulation; Oxidative stress; Oocyte; Mitochondria; Aging; Carnitine; Free radicals
资金
- Ministry of Education, Culture, Sports, Science, and Technology of Japan [16590252, 14370062]
- Grants-in-Aid for Scientific Research [16590252, 21700698, 14370062] Funding Source: KAKEN
We previously reported that superoxide generated in the ovary induces apoptosis of granulosa cells to break down follicular walls, thereby supporting ovulation in rodents, and suggested that oxidative stress underlies the mechanism of ovarian aging. To test this hypothesis, we successfully induced ovulation repeatedly in mice by sequentially administrating pregnant mare serum gonadotropin, human chorionic gonadotropin, and prostaglandin F2 alpha. Kinetic analysis revealed that the number of ovulated oocytes decreased significantly with repeated cycles of ovulation with a concomitant decrease in the gene expression of mitochondrial transcription factor A and nuclear respiratory factor 1 and an increase in oocytes having abnormally distributed mitochondria. Repeated ovulation decreased the amounts of mitochondrial DNA and increased 8-hydroxydeoxyguanosine in oocytes. Cell culture analysis of the in vivo fertilized oocytes revealed that their maturation from two cells to blastocyst was inhibited significantly by repeated ovulation. All these events induced by repeated ovulation were suppressed by oral administration of L-carnitine. These results suggest that oxidative stress associated with ovulation underlies the mechanism of ovarian aging and that L-carnitine may have therapeutic potential in patients with infertility and increased incidence of aneuploidy and to suppress impaired maturation of zygotes frequently observed in childbearing at an advanced age. (C) 2010 Elsevier Inc. All rights reserved.
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