Article
Biochemistry & Molecular Biology
Berken Cimen, Turgut Yesiloglu, Dicle Donmez, Yildiz Aka Kacar, Sezai Ercisli
Summary: This study aimed to develop local triploid hybrids using the embryo rescue technique in citrus. A total of 574 triploid plants were recovered from different crosses, and their genetic characteristics and stomatal traits were analyzed. SSR markers were successfully used to confirm the genotyping of triploid plants, which are currently undergoing field trials for yield and quality performances.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Agronomy
Vijayakumari Narukulla, Yogesh Lahane, Krutika Fiske, Shashi Pandey, Vasileios Ziogas
Summary: In this study, stable tetraploids were developed in citrus rootstock cultivars including Rough lemon, Rangpur lime, and Alemow through in vitro colchicine treatments. The treatment with 0.1% colchicine for 24 hours resulted in the highest tetraploid induction percentage (18.3%) in all the rootstock cultivars.
Article
Biology
Alessio De Magis, Melanie Kastl, Peter Brossart, Annkristin Heine, Katrin Paeschke
Summary: The study introduces a novel protocol using the G4-specific antibody BG4 for quantifying G4 structures with flow cytometry (BG-flow). It demonstrates the effectiveness of BG-flow in quantifying G4 levels in various cells, ranging from mouse to human, and suggests the combination of BG-flow with additional antibodies for cell surface markers to study G4 structures in subpopulations of cells for potential diagnostic applications.
Article
Agronomy
Lucy N. Gitonga, Glynis Cron, Kelsey L. Glennon, Andrew J. McConnachie, Marcus J. Byrne
Summary: The invasive species Campuloclinium macrocephalum in South Africa and Argentina exhibit different ploidy levels, with mainly triploids and tetraploids identified in South Africa, and diploids, triploids, tetraploids, and aneuploids found in Argentina. The distribution of these ploidy levels varies between the two countries, with tetraploids being more widespread in South Africa and triploids mainly found in the likely origin of the invasion. Different ploidy levels also show distinct phenotypic variation in the individuals of Campuloclinium macrocephalum.
Article
Biology
Neda Javadian, Ghasem Karimzadeh, Mohsen Sharifi, Ahmad Moieni
Summary: In this study, tetraploid callus cultures of Linum album, a plant endemic to Iran known for its medicinal properties, were established to increase the production of podophyllotoxin (PTOX). The tetraploid calli produced 2.1-fold more PTOX compared to diploid calli. The up-regulation of key genes involved in PTOX biosynthesis was observed in the tetraploid calli, indicating that autopolyploidy could be an effective strategy for enhancing PTOX production in L. album callus.
Article
Mycology
Kris Van Poucke, Annelies Haegeman, Thomas Goedefroit, Fran Focquet, Leen Leus, Marilia Horta Jung, Corina Nave, Miguel Angel Redondo, Claude Husson, Kaloyan Kostov, Aneta Lyubenova, Petya Christova, Anne Chandelier, Slavcho Slavov, Arthur de Cock, Peter Bonants, Sabine Werres, Jonas Oliva Palau, Benoit Marcais, Thomas Jung, Jan Stenlid, Tom Ruttink, Kurt Heungens
Summary: The genus Phytophthora contains economically and ecologically important plant pathogens, with hybrid species posing a serious threat to agriculture and natural ecosystems. By combining genotyping-by-sequencing and genome size estimation, researchers were able to identify and characterize previously described hybrid species and discover new ones, ultimately improving species identification and phylogenetic relations in an efficient and cost-effective manner.
Article
Plant Sciences
Gregor Kozlowski, Yann Fragniere, Benoit Clement, Conor Meade
Summary: This study estimated the genome sizes of different species in the genus Arenaria using flow cytometry and found that the subspecies Arenaria ciliata subsp. bernensis has the highest ploidy level. This high alpine endemic plant shows distinct differences in morphology, phylogeny, phenology, and ecology compared to other taxa, providing further support for its taxonomic distinctiveness. Upgrading its species status would greatly improve its conservation prospects in the face of climate warming.
Article
Multidisciplinary Sciences
Ryan J. Quinton, Amanda DiDomizio, Marc A. Vittoria, Kristyna Kotynkova, Carlos J. Ticas, Sheena Patel, Yusuke Koga, Jasmine Vakhshoorzadeh, Nicole Hermance, Taruho S. Kuroda, Neha Parulekar, Alison M. Taylor, Amity L. Manning, Joshua D. Campbell, Neil J. Ganem
Summary: Whole-genome doubling (WGD) in human cancers results in genetically unstable tetraploid cells that depend on specific signaling pathways and require the KIF18A gene for viability. Targeting vulnerabilities unique to WGD(+) cells presents a potential strategy for cancer therapy.
Article
Agronomy
Adina Y. Grossman, Mario Henrique Murad Leite Andrade, Ana Luisa Arantes Chaves, Marco Tulio Mendes Ferreira, Vania Helena Techio, Yolanda Lopez, Kevin Begcy, Kevin E. Kenworthy, Esteban F. Rios
Summary: This study investigated the USDA bermudagrass germplasm set, identifying ploidy levels and evaluating their impact on estimating genetic parameters. The findings will support future breeding efforts and selections for forage bermudagrass.
Article
Plant Sciences
Juneyd Alavi, Asad Maroufi, Ghader Mirzaghaderi
Summary: The study demonstrates that induction of tetraploid plants in fenugreek through embryo culture treated with trifluralin is a more efficient method compared with colchicine, as trifluralin treatment results in higher survival rates of embryos and potentially increases secondary metabolite content. The elevated transcript expression of key genes involved in diosgenin biosynthesis in tetraploid plants suggests that trifluralin-induced tetraploidization may be beneficial for improving yield and quality of secondary metabolites in fenugreek.
ACTA PHYSIOLOGIAE PLANTARUM
(2022)
Article
Gastroenterology & Hepatology
Mariia Taguer, B. Jesse Shapiro, Corinne F. Maurice
Summary: The research optimized the BONCAT method and FACS-Seq technique to identify translationally active members of the gut microbiota, revealing a more accurate way to study gut microbiota. The study found that bacteria with high nucleic acid content play a significant role in the gut microbiota, correlating with a subset of damaged bacteria, and physiologically distinct subsets may provide more informative insights than whole-community profiling.
Article
Horticulture
Matthew S. Lobdell, Kimberly Shearer
Summary: This study used flow cytometry and cytological analysis to investigate the genome size and ploidy of various Styrax species. The findings demonstrated the presence of different ploidy levels within the Styrax genus and provided information on the genome size of multiple species.
Article
Engineering, Environmental
Ruihua Cao, Qiqi Wan, Xiangqian Xu, Shiqi Tian, Gehui Wu, Jingyi Wang, Tinglin Huang, Gang Wen
Summary: A new method was established in this study to differentiate DNA damage and membrane damage of fungal spores, elucidating the disinfection mechanism during chemical disinfection. The repeatability and applicability of this method were confirmed, and it was further applied to explore the inactivation mechanism during chlorine-based disinfection.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Chemistry, Analytical
Yiyang Li, Boqian Wang, Zara Ahmad Khan, Jie He, Edwin Cheung, Wenqiong Su, Aiting Wang, Hui Jiang, Lai Jiang, Xianting Ding
Summary: This study introduces a method of mass cytometry profiling for analyzing samples with limited or rare cell subsets using a platinum-chimeric carrier cell strategy. The results demonstrate the effectiveness of this method in preserving rare target cells and performing comprehensive single-cell subtype classification analysis.
ANALYTICAL CHEMISTRY
(2023)
Article
Genetics & Heredity
Haluk Kulaz, Solmaz Najafi, Ruveyde Tuncturk, Murat Tuncturk, Marzough Aziz Albalawi, Adel I. Alalawy, Atif Abdulwahab A. Oyouni, Abdulrahman Alasmari, Peter Poczai, R. Z. Sayyed
Summary: In this study, the karyotype variations and genome sizes of four common bean genotypes in Turkey were investigated to determine the impact of geographic variables on these parameters. The results showed differences in karyotype and genome size among the genotypes, indicating the influence of climate change in the regions. This information can be valuable for the improvement of common beans and breeding programs.
Article
Environmental Sciences
Jianfeng Feng, Leif Chr Stige, Dag Olav Hessen, Zhiling Zuo, Lin Zhu, Nils Chr Stenseth
Summary: The study shows that ocean phytoplankton chlorophyll is significantly influenced by sea-surface temperatures, with effects varying based on baseline temperature levels and showing a shift from positive to negative effects at around 14 degrees Celsius.
GLOBAL BIOGEOCHEMICAL CYCLES
(2021)
Article
Limnology
Thomas R. Anderson, Dag O. Hessen, Daniel J. Mayor
Summary: The growth of marine copepods is limited by both food quantity and quality, with a stronger limitation by C element despite significant losses of N during metabolism. Developing mechanistic representations of zooplankton metabolism is crucial for improving the accuracy of biogeochemical models.
LIMNOLOGY AND OCEANOGRAPHY LETTERS
(2021)
Article
Environmental Sciences
Benjamin M. Kraemer, Rachel M. Pilla, R. Iestyn Woolway, Orlane Anneville, Syuhei Ban, William Colom-Montero, Shawn P. Devlin, Martin T. Dokulil, Evelyn E. Gaiser, K. David Hambright, Dag O. Hessen, Scott N. Higgins, Klaus D. Johnk, Wendel Keller, Lesley B. Knoll, Peter R. Leavitt, Fabio Lepori, Martin S. Luger, Stephen C. Maberly, Dorthe C. Mueller-Navarra, Andrew M. Paterson, Donald C. Pierson, David C. Richardson, Michela Rogora, James A. Rusak, Steven Sadro, Nico Salmaso, Martin Schmid, Eugene A. Silow, Ruben Sommaruga, Julio A. A. Stelzer, Dietmar Straile, Wim Thiery, Maxim A. Timofeyev, Piet Verburg, Gesa A. Weyhenmeyer, Rita Adrian
Summary: Long-term temperature changes in lakes lead to thermal habitat changes, exacerbated by seasonal and depth-related constraints. Tropical lakes show higher thermal non-overlap compared to lakes at other latitudes, and lakes with high biodiversity and endemism exhibit higher thermal habitat changes.
NATURE CLIMATE CHANGE
(2021)
Article
Marine & Freshwater Biology
Sabrina Schultze, Tom Andersen, Dag O. Hessen, Anders Ruus, Katrine Borga, Amanda E. Poste
Summary: Climate and land-use changes have impacts on individual and interconnected ecosystems. This study examines the dynamics of organic matter (OM) and nutrients in two Norwegian river-to-fjord systems and finds that changes in river inputs can lead to shifts in coastal carbon and nutrient cycling, affecting ecosystem structure and function.
ESTUARINE COASTAL AND SHELF SCIENCE
(2022)
Review
Obstetrics & Gynecology
Hans Ivar Hanevik, Dag O. Hessen
Summary: Humans and all species are shaped by evolution through natural selection. IVF, as a reproductive technology, alters selection pressures and may have long-term impacts on human evolution.
HUMAN REPRODUCTION UPDATE
(2022)
Article
Environmental Sciences
You-Ren Wang, Nina Buchmann, Dag O. Hessen, Frode Stordal, Jan Willem Erisman, Ane Victoria Vollsnes, Tom Andersen, Han Dolman
Summary: By analyzing data from 22 forest sites across Europe, we found that deposition of sulphur and nitrogen has significant impacts on Net Ecosystem Production (NEP) of forests. Sulphur deposition above 5 kg S ha(-1) yr(-1) can significantly reduce NEP, while nitrogen deposition around 22 kg N ha(-1) yr(-1) has the highest positive effect on NEP. Our results emphasize the importance of air quality management for maintaining the biogeochemical functions of forests and mitigating climate change.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Environmental Studies
Dag O. Hessen, Vigdis Vandvik
Summary: It is increasingly clear that climate sustainability relies not only on societal actions, but also on the functioning and responses of ecosystems. Climate change and ecosystem degradation are reducing the capacity of global ecosystems to provide services such as carbon sequestration and hydrological regulation. Climate change adaptation and mitigation strategies should focus on conserving and managing ecosystems to minimize climate hazards and risks.
WEATHER CLIMATE AND SOCIETY
(2022)
Article
Environmental Sciences
Thomas R. Anderson, Dag O. Hessen, Wendy C. Gentleman, Andrew Yool, Daniel J. Mayor
Summary: This study investigates the biogeochemical cycling of carbon and nitrogen by high-latitude copepods using a new individual-based stoichiometric model. The results highlight the importance of understanding the physiological utilization of lipids by copepods and suggest that the seasonal lipid pump may be comparable to carbon sequestration via sinking particles in the North Atlantic.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Biodiversity Conservation
Isolde Callisto Puts, Jenny Ask, Matthias B. Siewert, Ryan A. Sponseller, Dag O. Hessen, Ann-Kristin Bergstrom
Summary: Global change affects gross primary production in benthic and pelagic habitats of northern lakes by influencing catchment characteristics and lake water biogeochemistry. Changes in key environmental drivers manifest in light limitation of total GPP and the relative size of the benthic habitat. External inputs of inorganic carbon can influence lake productivity patterns independent of terrestrial DOC supply.
GLOBAL CHANGE BIOLOGY
(2022)
Article
Environmental Sciences
You-Ren Wang, Dag O. Hessen, Bjorn H. Samset, Frode Stordal
Summary: Global surface temperature has been setting new record highs, posing increasing challenges for societies and ecosystems worldwide. In this study, two independent datasets, one from remote-sensing and one from surface modeling and observations, are analyzed to estimate land surface temperature change rates. The results show that the global average land surface temperature rate of change was 0.26-0.34°C per decade during the period 2001-2020, with significant regional variations. The Arctic, Europe, and Russia experienced the most significant warming.
REMOTE SENSING OF ENVIRONMENT
(2022)
Article
Ecology
Bjorn Walseng, Joel M. Durant, Dag O. Hessen, Kurt Jerstad, Anna L. K. Nilsson, Ole W. Rostad, Tore Slagsvold
Summary: Mating strategies in white-throated dippers were investigated based on a comprehensive dataset. The study revealed that a small percentage of breeding events were performed by polygynous males, and the occurrence of polygyny remained stable throughout the study period. Surprisingly, there was no difference in territory quality between polygynous and monogamous males. This study also found that the age and proximity of the females mated to polygynous males differed from those mated to monogamous males.
ECOLOGY AND EVOLUTION
(2022)
Article
Environmental Sciences
Nicolas Valiente, Alexander Eiler, Lina Allesson, Tom Andersen, Francois Clayer, Camille Crapart, Peter Dorsch, Laurent Fontaine, Jan Heuschele, Rolf D. Vogt, Jing Wei, Heleen A. de Wit, Dag O. Hessen
Summary: Boreal lakes, the most abundant lakes on Earth, are experiencing browning due to changes in acid rain deposition, climate, and land use. This browning affects aquatic communities, ecosystem processes, and greenhouse gas emissions. A study conducted in southeastern Norway showed that lake size, precipitation, and terrestrial primary production in the watershed are key determinants of greenhouse gas saturation in boreal lakes. The findings highlight the importance of incorporating multiple explanatory parameters in prediction models for better understanding and extrapolation across the boreal biome.
FRONTIERS IN ENVIRONMENTAL SCIENCE
(2022)
Article
Multidisciplinary Sciences
Lina Allesson, Nicolas Valiente, Peter Dorsch, Tom Andersen, Alexander Eiler, Dag O. Hessen
Summary: Lakes play a significant role in the global climate, but the environmental drivers of CO2 concentrations along latitudinal and climate gradients are not well understood. This study investigated boreal, sub-Arctic, and high-Arctic lakes in Norway to better understand net heterotrophy and gas balance. The results showed that CO2 saturation levels varied more than O2 saturation levels, and the CO2:O2 ratio was mainly influenced by DOC concentration in boreal lakes and conductivity in sub-Arctic and high-Arctic lakes.
SCIENTIFIC REPORTS
(2022)
Article
Marine & Freshwater Biology
Sabrina Schultze, Tom Andersen, Nina Knudtzon, Anders Ruus, Jan T. Rundberget, Steven J. Brooks, Amanda Poste, Dag O. Hessen, Katrine Borga
Summary: The export of terrestrial dissolved organic matter (terrDOM) to coastal marine ecosystems can affect local filter feeders and the local food web. In this study, blue mussels were exposed to different types of DOM along with teflubenzuron, a commonly used aquaculture medicine. The results showed that the accumulation of teflubenzuron was influenced by the type of DOM, but the differences between DOM types were not considered environmentally relevant.
AQUATIC TOXICOLOGY
(2023)
Article
Medicine, General & Internal
Hans Ivar Hanevik, Dag Olav Hessen
TIDSSKRIFT FOR DEN NORSKE LAEGEFORENING
(2022)