期刊
FOOD CONTROL
卷 23, 期 1, 页码 245-250出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodcont.2011.07.023
关键词
Melamine; Dairy products; HILIC; Amino column
资金
- National Natural Science Foundation of China [30771509]
A HPLC method using a NH2 column for determination of melamine was reported, with acetonitrile-H3PO4 (15 mmol L-1. pH = 6.5) (V/V = 88:12) as mobile phase and UV detection at 215 nm. The tested parameters included mobile phase composition, percentage of organic solvent, and buffer concentration, and pH value and UV detection wavelength. Good linearity was observed within the concentration range of 0.005-32 mg L-1 (n = 7, r = 0.9997), the detection limit was 0.003 mg L-1 (S/N = 3). The chromatography mode was considered to be hydrophilic interaction chromatography (HILIC). The method was successfully applied to determine melamine in several dairy products, with acetonitrile as extract solvent of melamine and also as protein precipitant. The spike recovery of melamine was within 73-96%. (C) 2011 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据