Article
Food Science & Technology
Qiyuan Jing, Shijian Liu, Yang Song, Xiaoqi Tao
Summary: In this study, a TaqMan qPCR method based on the cytochrome b gene was established for quantitative detection of beef tallow. A normalized Delta Cq method based on Cyt b and 18S rDNA was also created for quantitative analysis of oils, which was validated on simulated oil samples.
Article
Virology
Yi Huang, Shuqi Xiao, Zhiming Yuan
Summary: The study compared and evaluated the sensitivity, reproducibility, and specificity of 23 in-house assays for ebolavirus detection, finding that targeting the more conservative NP gene may offer better sensitivity for EVD case definition and quantitative measurement. This analysis could contribute to the standardization of ebolavirus detection and quantification assays, providing a better understanding of results and making implementation easier, particularly during outbreaks.
Article
Agronomy
Jiao He, Mei-Ling Sun, De-Wei Li, Li-Hua Zhu, Jian-Ren Ye, Lin Huang
Summary: The TaqMan real-time PCR targeting the ApMat gene has the potential to provide rapid, specific, and reliable molecular detection of C. fructicola, C. gloeosporioides, C. siamense, and C. cangyuanense. This study also offers a reference solution for identifying species within the Colletotrichum gloeosporioides species complex (CGSC) that share similar morphological characteristics.
PEST MANAGEMENT SCIENCE
(2023)
Article
Microbiology
Alexandra Puertolas, Peter J. M. Bonants, Eric Boa, Steve Woodward
Summary: Numerous outbreaks of Phytophthora and Pythium diseases have occurred in Europe due to introduction of contaminated ornamental plants. Detection and identification of pathogens are crucial. Results showed that oomycetes were isolated from 89.9% of plants using classical techniques, and real-time PCR demonstrated that oomycetes were associated with all plants tested.
Article
Agriculture, Dairy & Animal Science
Min Zheng, Su Lin, Shizhong Zhang, Xiuqin Chen, Dandan Jiang, Shaoying Chen, Shao Wang, Shilong Chen
Summary: A novel TaqMan-based real-time PCR approach was developed to reliably detect and diagnose H146-like GCV, enabling real-time diagnosis of infections and providing a greater understanding of the epidemiology and clinical presentation of the disease.
Article
Food Science & Technology
M. A. Motalib Hossain, Siti Aimi Sarah Zainal Abidin, Aishah Bujang, Mohd Nasir Taib, Suresh Sagadevan, Mohd Rafie Johan, Nina Naquiah Ahmad Nizar
Summary: Meat and meat products are highly sought after globally for their high-quality protein and nutrients, but the industry faces challenges such as food fraud, mislabeling, and zoonotic diseases. Quantitative detection of the species origin using methods like PCR is crucial for food control. This review focuses on the design, development, and applications of TaqMan probe-based mqPCR techniques for authenticating meat products, providing a reference for academia and industry to improve existing assays.
Article
Veterinary Sciences
Siti Tasnim Makhtar, Sheau Wei Tan, Nur Amalina Nasruddin, Nor Azlina Abdul Aziz, Abdul Rahman Omar, Farina Mustaffa-Kamal
Summary: A specific TaqMan-based real-time RT-PCR assay targeting the N gene of FeMV was developed in this study, showing higher detection rate and sensitivity in early detection of FeMV infection.
BMC VETERINARY RESEARCH
(2021)
Article
Veterinary Sciences
Yuelin Liu, Libing Liu, Jinfeng Wang, Ting Wang, Yaxin Gao, Xiaoxia Sun, Wanzhe Yuan, Ruiwen Li, Jianchang Wang
Summary: In this study, a new Taqman-based real-time RT-PCR method was developed for the detection of Bovine kobuvirus (BKV), which can be used efficiently and specifically for BKV detection and epidemiological research.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Da Zhang, Junhuang Wu, Jianfei Sun, Caixia Bai, Fazhi Xu, Pengfei Duan, Yong Wang
Summary: A TaqMan-based real-time PCR assay was established to detect Duck circovirus, showing high sensitivity, specificity, and repeatability, making it suitable for clinical use.
Article
Microbiology
Amro Hashish, Avanti Sinha, Yuko Sato, Nubia R. Macedo, Mohamed El-Gazzar
Summary: This article describes the development of an improved detection method for Ornithobacterium rhinotracheale (ORT). The modified method exhibits higher efficiency, sensitivity, and specificity, enabling sensitive and efficient diagnosis of ORT from clinical samples.
Article
Biochemical Research Methods
Babu K. Nath, Shubhagata Das, Tridip Das, Jade K. Forwood, Shane R. Raidal
Summary: TaqMan qPCR is a robust and reliable technique for detecting and quantifying target DNA copies. This study reports the development and validation of a highly sensitive TaqMan qPCR for the detection of highly diverse PiCV in pigeon samples with excellent reproducibility, specificity, and sensitivity.
JOURNAL OF VIROLOGICAL METHODS
(2022)
Article
Agriculture, Dairy & Animal Science
Jinpeng Zhang, Huanru Fu, Cuiteng Chen, Jinxiu Jiang, Yusheng Lin, Bin Jiang, Lin Lin, Qilin Hu, Chunhe Wan
Summary: PiMeV infection was detected in fecal samples of pigeons and a sensitive and specific TaqMan-qPCR method was established. The study also suggests that PiMeV may be vertically transmitted in racing pigeon embryos.
Article
Fisheries
Han Lin, Jingyang Sun, Jie Ma, Zhendong Qin, Biao Jiang, Wei Li, Qing Wang, Youlu Su, Li Lin, Chun Liu
Summary: This study investigated the spread and replication of Klebsiella pneumoniae in infected bullfrog tissues. A qPCR assay was developed to detect and quantify the bacteria, with the spleen identified as the major site for K. pneumoniae replication. Additionally, the mucus assay showed potential as a noninvasive tool for the early surveillance and prevention of K. pneumoniae in bullfrogs.
Article
Food Science & Technology
Liang Guo, Chun-Dong Li, Guo-Qiang Liu, Jian-Xing Luo, Wei-Liang Xu, Yuan-Sheng Guo
Summary: This study investigated SNPs in the FecB locus of Xilingol indigenous sheep breeds using novel techniques, laying the groundwork for future authentication and breeding strategies.
FOOD SCIENCE & NUTRITION
(2022)
Article
Biochemical Research Methods
Zeinab Alsaheli, Ali Abdallah, Ornella Incerti, Ahmad Shalaby, Sahar Youssef, Michele Digiaro, Toufic Elbeaino
Summary: Singleplex and multiplex real-time RT-PCR assays were developed to detect seven fig-infecting viruses, and a large-scale survey in Egypt showed a high prevalence of mixed infections, with FMV being the most commonly observed virus.
JOURNAL OF VIROLOGICAL METHODS
(2021)
Article
Food Science & Technology
Ewelina Bigoraj, Iwona Kozyra, Agnieszka Kaupke, Zbigniew Osinski, James Lowther, Artur Rzezutka
Summary: The aim of this study was to assess the prevalence of human enteric viruses in mussels sold on the Polish market and study the fecal contamination of shellfish. The study found that human enteric viruses were commonly present in shellfish, with norovirus being the most frequently detected. The study also observed a seasonal distribution pattern of viruses in shellfish samples.
Editorial Material
Food Science & Technology
Bojan Blagojevic, Lis Alban Denmark
Article
Food Science & Technology
Indy Magnus, Fatemeh Abbasi, Hugo Thienpont, Lien Smeesters
Summary: Food safety and quality are increasingly important in our society, driving the development of novel optical food sensing technologies. However, current optical sensing technologies are often limited in their sensitivity and product variability. In this study, we developed a novel pistachio nut screening methodology using fluorescence spectroscopy and advanced chemometrics to simultaneously detect shells, tree parts, and aflatoxins. By optimizing hardware and software parameters, we achieved a high classification accuracy for healthy pistachio kernels.
Article
Food Science & Technology
Xiaodong Wang, Jingqiang Yu, Haixia Wu, Leiying Wu, Wuyunga Borjihan, Cong Wang, Munkhjargal Burenjargal, Alideertu Dong
Summary: This study developed a novel photon-phonon coupling (PPC) cold sterilization technology and compared its effects with low-temperature pasteurization and microwave ultraviolet. The results showed that PPC effectively reduced bacterial count in raw milk with minimal impact on milk quality and nutritional composition.
Review
Food Science & Technology
Riyadh Abdulmalek Hassan, Sharina Abu Hanifah, Lee Yook Heng, Fawaz Al-badaii, Alizar Ulianas
Summary: This study used bibliometric visualisation map analysis to explore the publication outputs, trends, and hotspots for endocrine disruptors (EDMs) biosensor development in relation to mycotoxins. The analysis showed that the current literature primarily focuses on developing biosensors for detecting highly toxic and widely distributed mycotoxins in food, but there needs to be more focus on biosensors for detecting mycotoxins known as endocrine disruptors.
Article
Food Science & Technology
Yajie Zheng, Jianpeng Dou, Changpo Sun, Hujun Liu, Shipei Zhang, Wenfu Wu
Summary: This study investigated the efficiency of modified SA/ll-glucan/Mt microsphere adsorbents in removing DON and ZEN from CSL. The microspheres showed high adsorption capacity, and simple and rapid regeneration was achieved without affecting the removal efficiency of mycotoxins from CSL.
Article
Food Science & Technology
Carlos Adelantado, Jose Angel Salatti-Dorado, Carlos Herrera, Lourdes Arce, Maria Jose Cardador
Summary: High-performance-ion mobility spectrometry (HPIMS) was used for the first time in the analysis of olive oil samples, following a simple liquid-liquid extraction procedure. The developed methodology is user-friendly, fast, and inexpensive, complying with the principles of green chemistry. The proposed approach, combined with multivariate analysis, showed potential in classifying olive oil samples based on their organoleptic quality, with high accuracy rates.
Article
Food Science & Technology
Jia-Wen Wei, Jia-Rong He, Shi-Yi Chen, Yi-Han Guo, Xuan-Zhu Huo, Nuan Zheng, Shuo-Hui Cao, Yao-Qun Li
Summary: Polycyclic aromatic hydrocarbons (PAHs) are a major cause of human cancer. This study developed a quantitative analysis method for PAH4 using the back propagation neural network (BPNN) algorithm and constant wavelength synchronous fluorescence (CWSF) spectra as the data sets. The method can predict the concentrations of PAH4 in edible oil samples without preprocessing or pre-separation. It has been proven to be a powerful tool for the rapid detection of PAH4.
Article
Food Science & Technology
Liyun Chen, Xiumei Li, Jingyi Chen, Ranxun Lin, Yuhan Mai, Yuxin Lin, Guodong Wang, Zheng Chen, Wei Zhang, Jiang Wang, Cai Yuan, Longguang Jiang, Peng Xu, Mingdong Huang
Summary: Food spoilage is primarily caused by microbial contamination. Curcumin has shown moderate antimicrobial activity as a food preservative. This study formulates curcumin with zinc acetate to enhance its efficacy, resulting in Cur-Zn with increased antimicrobial activity against multiple food-contaminating bacteria and fungi. With light illumination, the antimicrobial activity of Cur-Zn is further enhanced due to the photodynamic inactivation (PDI) effect.
Article
Food Science & Technology
Marta Pilar Ortiz-Moriano, Gonzalo Machado-Schiaffino, Eva Garcia-Vazquez, Alba Ardura
Summary: Mislabelling in the global fisheries sector raises concerns about the identity, safety, and sustainability of seafood products. This study examined mislabelling in shrimps and prawns sold in the Spanish market and assessed potential risks of heavy metal ingestion.
Article
Food Science & Technology
Doyeon Kim, Jinwoo Kim, Minsik Kim
Summary: This study demonstrates the enhanced bactericidal activity of engineered lysins fused with amphipathic antimicrobial peptides against Gram-negative bacterium Cronobacter sakazakii. The fusion lysins are capable of disrupting bacterial cells and significantly reducing the risk of infection within a certain range of temperature and pH. Additionally, the study also shows the bactericidal effect of these fusion lysins against other Gram-negative pathogens.
Article
Food Science & Technology
Seul-Ki Park, Kyung-Jin Cho, Du-Min Jo, Young-Mog Kim
Summary: This study presents a new approach for detecting pathogenic Vibrio spp. in contaminated seafood, which combines PCR with a simplified DNA extraction process and pre-culturing step. The method has been validated and shown to be efficient, time-saving, and highly sensitive, offering a potential alternative for improving safety measures in the food industry.
Article
Food Science & Technology
Suwimol Surareungchai, Chaleeda Borompichaichartkul, Chitsiri Rachtanapun, Nutthachai Pongprasert, Pongphen Jitareerat, Varit Srilaong
Summary: This study builds upon previous research and focuses on risk assessment and analysis in the organic salad industry. The results show that previous risk matrix models can be adapted for various raw materials, and preventive control plans can be applied across different aspects of the production process.
Article
Food Science & Technology
Xuan Zhang, Changling Fang, Xiaoyi Lou, Feng Han, Siman Li, Yongfu Shi, Dongmei Huang
Summary: This study aimed to assess the levels of Biogenic amines (BAs) in four common food types in the Chinese market. It was found that cheese and seafood derived product showed higher levels of BAs, and the histamine level in mackerel and yellow rice wine exceeded the limits.
Article
Food Science & Technology
Martina Foschi, Alessandra Biancolillo, Federico Marini, Francesco Cosentino, Francesca Di Donato, Angelo Antonio D'Archivio
Summary: This study aimed to develop a potentially non-destructive and routine-compatible method for the geographical traceability of high-valued Italian chickpeas. By analyzing the spectral information of the outer and inner parts of the kernels, it was found that the spectral analysis of the outer part yielded accurate and reliable results, indicating the possibility of non-destructive authentication.