Article
Food Science & Technology
Nan Jiang, Liuqing Wang, Dongmei Jiang, Meng Wang, Hongmei Liu, Hang Yu, Weirong Yao
Summary: This study evaluated the impact of eugenol on OTA production and mycelial growth of A. carbonarius. The results showed that eugenol significantly inhibited OTA production and caused morphological changes in spores and mycelia. Transcriptomic analysis identified differentially expressed genes associated with eugenol treatment. Additionally, eugenol disrupted cell integrity and induced oxidative stress in A. carbonarius.
Article
Agriculture, Multidisciplinary
Xinyu Cai, Boqiang Li, Xue Li, Hui Dang, Di Wang, Zhifei Pei, Xuan Feng, Xueyan Ren, Qingjun Kong
Summary: In this study, the inhibitionsites of ochratoxin A (OTA) synthesis pathway in Aspergillus carbonarius by stilbenes were accurately identified from the perspective of oxidative stress. The relationship between the physical and chemical properties of natural polyphenolic substances and their biochemical properties of antitoxin was comprehensively explored. Ultra-high-performance liquid chromatography and triple quadrupole mass spectrometry were utilized for real-time tracking of pathway intermediate metabolite content, facilitated by the synergistic effect of Cu2+-stilbenes self-assembled carriers. The findings provide a theoretical basis for the extensive and efficient application of natural polyphenolic substances for postharvest disease control and quality assurance of grape products.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Xinyu Cai, Jianrui Qi, Zhe Xu, Lingxuan Huang, Yihan Li, Xueyan Ren, Qingjun Kong
Summary: This study demonstrated the toxic effects of stilbenes on fungi, with different structural variations influencing their antifungal properties. Pterostilbene with methoxy exhibited the strongest antifungal activity, followed by piceatannol with a catechol structure, and finally resveratrol. Stilbenes inhibited the growth and germination of A. carbonarius mycelia, and their antitoxic activities were positively correlated with their antifungal activities. Stilbenes reduced OTA/OTB production and downregulated biosynthetic genes and regulatory factors associated with OTA production.
Article
Food Science & Technology
Yan Wang, Yong Zheng, Aiyun Zhou, Jing Neng, Di Wu, Xiao Li Shen, Xiuyu Lou, Kai Yang
Summary: Pulsed light technology has been proven to effectively control fungi in postharvest fruit. This study found that pulsed light inhibited the growth of Aspergillus carbonarius in a dose-dependent manner. Pears treated with pulsed light showed reduced scab diameter and decreased levels of ergosterol and OTA.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Biochemistry & Molecular Biology
Mariana Paiva Rodrigues, Aguida Aparecida de Oliveira, Gabriela Lago Biscoto, Priscila Natalia Pinto, Raul Roque de Souza Dias, Lauranne Alves Salvato, Luiz Antonio Moura Keller, Lilia Renee Cavaglieri, Carlos Alberto da Rocha Rosa, Kelly Moura Keller
Summary: The inhibitory effect of essential oils and plant extracts from 10 different plants cultivated in Brazil on the mycelial growth of Aspergillus westerdijkiae and A. carbonarius was evaluated. Oregano essential oil, rosemary essential oil, and clove ethanolic extract showed the best results in terms of growth parameters and volume of oil/extract obtained.
Article
Food Science & Technology
Stefanos I. Testempasis, Nathalie N. Kamou, Emmanouil-Nikolaos Papadakis, Urania Menkissoglu-Spiroudi, George S. Karaoglanidis
Summary: The study investigated the impact of conventional and organic farming systems on Black Aspergilli population structure in Greek vineyards and found that farming system may affect the species composition within the vineyard, with a higher risk of selection of Aspergillus strains with higher mycotoxigenic capacity in conventional vineyards.
Article
Chemistry, Multidisciplinary
Najmeh Yazdanfar, Trias Mahmudiono, Yadolah Fakhri, Amir Hossein Mahvi, Parisa Sadighara, Ali Akbar Mohammadi, Mahmood Yousefi
Summary: This study detected ochratoxin A (OTA) in coffee using high-performance liquid chromatography with fluorescence detectors and calculated the OTA content, Margin of exposure (MOEs), and Hazard quotient (HQ) in different types of coffee. The results showed that the OTA concentration in classic and instant coffee was below the maximum limit defined by the European Union legislation. The daily intakes of OTA through classic and instant coffee were also lower than the proposed tolerance daily intake. However, consumers of instant coffee are at a cancer risk based on neoplastic effects.
ARABIAN JOURNAL OF CHEMISTRY
(2022)
Article
Food Science & Technology
G. A. Dominguez-Gutierrez, I. Perraud-Gaime, H. Escalona-Buendia, N. Durand, E. I. Champion-Martinez, R. R. Fernandez-Martinez, G. Saucedo-Castaneda, G. Rodriguez-Serrano
Summary: This study optimized an inexpensive culture medium for Lactiplantibacillus plantarum MZ809351 strain B31, which resulted in a biomass concentration similar to the reference LAB culture medium. Sodium acetate and magnesium sulfate were the most significant factors affecting biomass concentration. Additionally, the inhibitory effects of LAB strains on the growth and production of OTA in a fungus were tested, and it was found that LAB can be used as a biological control agent against ochratoxigenic fungi in food.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Article
Food Science & Technology
Tehmina Anjum, Wajiha Iram, Mazhar Iqbal, Mateen Abbas, Waheed Akram, Guihua Li
Summary: The aqueous extracts of Mentha arvensis leaves show significant potential in degrading aflatoxin B1 and B2, with optimized conditions resulting in high degradation rates and low-toxicity products.
Article
Chemistry, Multidisciplinary
Paola Giorni, Leilei Zhang, Luigi Bavaresco, Luigi Lucini, Paola Battilani
Summary: Limited knowledge exists regarding the susceptibility of different grape varieties to ochratoxin A (OTA)-producing fungi. This study explored the susceptibility of six grape varieties to Aspergillus carbonarius in terms of OTA contamination and metabolome modulation. Significant differences were observed across cultivars, with varying incidences of moldy berries and levels of OTA contamination. Metabolomic analysis revealed cultivar-specific and general responses, providing valuable insights into the underlying mechanisms and facilitating preventive actions.
Review
Biochemistry & Molecular Biology
Alexandros Mavrommatis, Elisavet Giamouri, Savvina Tavrizelou, Maria Zacharioudaki, George Danezis, Panagiotis E. Simitzis, Evangelos Zoidis, Eleni Tsiplakou, Athanasios C. Pappas, Constantinos A. Georgiou, Kostas Feggeros
Summary: Mycotoxins are a significant threat to both the agriculture sector and the health of humans and animals, with detrimental effects on the antioxidant systems in livestock. The degree of oxidative stress is influenced by various factors, including contamination duration, co-contamination, toxin levels, animal characteristics, and production stage. A more comprehensive monitoring mechanism is urgently needed to address the damaging effects of mycotoxins on animal products.
Article
Food Science & Technology
Haiyong Zhang, Gang Wang, Qingli Yang, Xu Yang, Yongquan Zheng, Yang Liu, Fuguo Xing
Summary: The research found that UV-B light significantly inhibited fungal growth, while blue and violet light effectively inhibited the production of OTA. These results suggest that light could be used as a potential target for strategies in the prevention and control of ochratoxigenic fungi.
Article
Food Science & Technology
Ruifen Kang, Honglei Qu, Yanxin Guo, Mengjie Zhang, Tianze Fu, Shimeng Huang, Lihong Zhao, Jianyun Zhang, Cheng Ji, Qiugang Ma
Summary: Ochratoxin (OTA) is found in various foods and feeds, causing harmful effects on animals and humans. This study investigates the toxicokinetics of OTA-contaminated materials in Dezhou male donkeys. Donkeys were orally administered a single dose of 2500 μg OTA/kg BW from Aspergillus ochraceus culture material. The concentration of OTA in plasma was measured at different time points, and the excretion of OTA in urine and feces was quantified. The results showed that the maximum concentration of OTA in plasma was observed at 12 hours after administration, with an average value of 10.34 μg/mL. The total excretion in urine and feces accounted for approximately 10% of the intake within 120 hours.
Article
Food Science & Technology
Zsuzsanna Szoke, Bianka Babarczi, Miklos Mezes, Istvan Lakatos, Miklos Poor, Eszter Fliszar-Nyul, Miklos Oldal, Arpad Czeh, Kornelia Bodo, Gyorgy Nagyeri, Szilamer Ferenczi
Summary: Mycotoxins are bioaccumulative contaminants that affect animals and humans. There is a need for dedicated, sensitive, reliable, and practical assays to detect and quantify the accumulated mycotoxin levels. In this study, we successfully developed and modified assays, such as high-performance liquid chromatography with fluorescence detection, enzyme-linked immunosorbent assay, and flow cytometry assays, to measure the accumulated mycotoxin levels in mice exposed to Ochratoxin A. The results showed positive correlations between the exposure doses and accumulated mycotoxin levels. Among the assays, high-performance liquid chromatography had the highest sensitivity. Blood samples contained higher levels of mycotoxins than liver and kidney samples.
Article
Environmental Sciences
Zahra Zareshahrabadi, Mahdieh Karimirad, Keyvan Pakshir, Robab Bahmyari, Marjan Motamedi, Hasti Nouraei, Kamiar Zomorodian
Summary: Spices are essential in the diets of millions worldwide, but can be contaminated by fungi and mycotoxins. This study analyzed AFTs and OTA contamination in 80 spice samples, with the majority showing contamination, especially in curry samples. Only a small percentage of samples exceeded acceptable limits for AFTs and OTA.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Editorial Material
Plant Sciences
M. Rosati, M. Bogoescu, D. Spadaro
Article
Entomology
Valentina Candian, Marco Giuseppe Pansa, Karin Santoro, Davide Spadaro, Rossella Briano, Cristiana Peano, Luciana Tavella, Rosemarie Tedeschi
Summary: The use of exclusion nets significantly reduced pest populations, resulting in the production of healthier fruits with a strong reduction in insecticide treatments and related costs without any negative impact on postharvest rots, fruit quality, or nutraceutical properties.
Article
Microbiology
Ahmed Abdelfattah, Shiri Freilich, Rotem Bartuv, V. Yeka Zhimo, Ajay Kumar, Antonio Biasi, Shoshana Salim, Oleg Feygenberg, Erik Burchard, Christopher Dardick, Jia Liu, Awais Khan, Walid Ellouze, Shawkat Ali, Davide Spadaro, Rosario Torres, Neus Teixido, Okan Ozkaya, Andreas Buehlmann, Silvana Vero, Pedro Mondino, Gabriele Berg, Michael Wisniewski, Samir Droby
Summary: The first worldwide study on the apple fruit microbiome revealed significant variation in fungal diversity based on geographical location, with several beneficial microbial taxa forming the global core microbiome of apple fruit. This foundational information can be utilized for managing fruit quality and safety, and reducing losses due to postharvest pathogens.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Agronomy
Maria Aragona, Lidia Campos-Soriano, Edoardo Piombo, Elena Romano, Blanca San Segundo, Davide Spadaro, Alessandro Infantino
Summary: The study focused on the early interaction between Fusarium fujikuroi and rice roots, revealing that resistant cultivars trigger a hypersensitive response at the infection site and the expression of Chitinase1 gene is greatly increased in the resistant cultivar.
EUROPEAN JOURNAL OF PLANT PATHOLOGY
(2021)
Editorial Material
Plant Sciences
S. Prencipe, D. Spadaro
Article
Plant Sciences
F. Savian, S. Prencipe, N. Filippini, L. Nari, M. Martini, P. Ermacora, D. Spadaro
Summary: Research has shown that Kiwifruit vine decline syndrome may be caused by an interaction between multiple soil-borne pathogens, mainly oomycetes, and waterlogging. Experiment results demonstrate that P. chamaehyphon is pathogenic to KVDS, raising questions about the role of waterlogging in the etiology of KVDS.
Editorial Material
Plant Sciences
M. Waqas, V Guarnaccia, D. Spadaro
Article
Plant Sciences
Simona Prencipe, Giovanna Roberta Meloni, Luca Nari, Giada Schiavon, Davide Spadaro
Summary: In this study, black spot pathogens were isolated from symptomatic leaves and fruits of European pear, and their pathogenicity and characteristics were evaluated. The study found that the pathogens were present in both fruit and leaves, and produced multiple toxins. The results suggest potential risks to human health associated with black spot. This study also reports for the first time the infection of European pear by black spot pathogens in Italy.
Article
Plant Sciences
Simona Prencipe, Silvia Valente, Luca Nari, Davide Spadaro
Summary: The emerging pathogen Ramularia mali is causing dry lenticel rot in apple fruit. A SYBR Green qPCR assay was developed for the detection and quantification of R. mali in apple fruit. The assay showed good specificity, sensitivity, repeatability, and reproducibility, and can be used to clarify the life cycle of the pathogen for effective disease management.
Article
Biochemistry & Molecular Biology
Fabio Buonsenso, Giada Schiavon, Davide Spadaro
Summary: Biofumigation with slow-release diffusers of essential oils from basil, oregano, savoury, thyme, lemon, and fennel was effective in inhibiting the growth of Penicillium expansum, the pathogen causing blue mould of apples. The greatest reduction in blue mould was observed with lemon and oregano essential oils at 1.0% concentration after 60 days of storage and subsequent shelf-life. The essential oils did not have any phytotoxic effects on the apples during the storage period.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agronomy
Marco Garello, Edoardo Piombo, Simona Prencipe, Giada Schiavon, Lorenzo Berra, Michael Wisniewski, Samir Droby, Davide Spadaro
Summary: By measuring fruit disease incidence and using metabarcoding, this study characterized the microbial communities of apple fruit and revealed the epidemiology of white haze and dry lenticel rot. The study found that R. mali first appeared as an endophyte and then transitioned to an epiphyte, while Golubevia was the most abundant epiphyte associated with white haze. Additionally, significant differences in richness and composition were observed among different tissue, time points, and cultivars. Overall, this study provides insights into the diseases and suggests the potential of fruit metabarcoding as a tool for disease assessment and prediction.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2023)
Article
Microbiology
Simona Prencipe, Giada Schiavon, Marco Rosati, Luca Nari, Leonardo Schena, Davide Spadaro
Summary: Since 2012, the kiwifruit vine decline syndrome (KVDS) has been affecting Italian kiwifruit orchards. The presence of Phytopythium spp. has been identified in KVDS-affected orchards in north-western Italy. Inoculation experiments demonstrated the pathogenicity of P. vexans, P. litorale, P. chamaehyphon, and P. helicoides in potted plants. The study suggests that different species of Phytopythium play a significant role in the establishment and development of KVDS.
Article
Microbiology
Giada Schiavon, Marco Garello, Simona Prencipe, Giovanna Roberta Meloni, Fabio Buonsenso, Davide Spadaro
Summary: Biofumigation with thyme, savoury, and basil essential oils effectively inhibits the development of grey mould rot in apples caused by Botrytis cinerea. Thyme and savoury oils at 1% concentration significantly reduce the incidence of grey mould rot after 60 days of storage. Biofumigation also alters the species and abundance of microorganisms on the apple surface and inside the fruit.
Article
Microbiology
Muhammad Waqas, Simona Prencipe, Vladimiro Guarnaccia, Davide Spadaro
Summary: This study is the first characterization of Alternaria spp. associated with black rot of cherries in Italy, identifying A. arborescens species complex (AASC) and A. alternata as the main pathogens. All tested strains showed pathogenicity on sweet cherries.
Article
Biotechnology & Applied Microbiology
Francesco Ferrero, Simona Prencipe, Ernesto Tabacco, Ilario Ferrocino, Giorgio Borreani, Davide Spadaro
Summary: This study aimed to investigate the effects of a prothioconazole- and tebuconazole-based fungicide on the yield and quality of whole-crop corn (WCC) and high-moisture ear corn (HMC) silages, as well as on the dynamics of the fungal community from harvest to aerobic exposure. The results showed that the fungicide increased yield per hectare but did not consistently affect the nutritional characteristics of the silages. The main factors influencing the silage quality were the conservation time, regardless of the fungicide treatment. Additionally, the dominant fungal species changed from before ensiling to after silo opening and aerobic exposure.
JOURNAL OF APPLIED MICROBIOLOGY
(2023)
Article
Food Science & Technology
Ewelina Bigoraj, Iwona Kozyra, Agnieszka Kaupke, Zbigniew Osinski, James Lowther, Artur Rzezutka
Summary: The aim of this study was to assess the prevalence of human enteric viruses in mussels sold on the Polish market and study the fecal contamination of shellfish. The study found that human enteric viruses were commonly present in shellfish, with norovirus being the most frequently detected. The study also observed a seasonal distribution pattern of viruses in shellfish samples.
Editorial Material
Food Science & Technology
Bojan Blagojevic, Lis Alban Denmark
Article
Food Science & Technology
Indy Magnus, Fatemeh Abbasi, Hugo Thienpont, Lien Smeesters
Summary: Food safety and quality are increasingly important in our society, driving the development of novel optical food sensing technologies. However, current optical sensing technologies are often limited in their sensitivity and product variability. In this study, we developed a novel pistachio nut screening methodology using fluorescence spectroscopy and advanced chemometrics to simultaneously detect shells, tree parts, and aflatoxins. By optimizing hardware and software parameters, we achieved a high classification accuracy for healthy pistachio kernels.
Article
Food Science & Technology
Xiaodong Wang, Jingqiang Yu, Haixia Wu, Leiying Wu, Wuyunga Borjihan, Cong Wang, Munkhjargal Burenjargal, Alideertu Dong
Summary: This study developed a novel photon-phonon coupling (PPC) cold sterilization technology and compared its effects with low-temperature pasteurization and microwave ultraviolet. The results showed that PPC effectively reduced bacterial count in raw milk with minimal impact on milk quality and nutritional composition.
Review
Food Science & Technology
Riyadh Abdulmalek Hassan, Sharina Abu Hanifah, Lee Yook Heng, Fawaz Al-badaii, Alizar Ulianas
Summary: This study used bibliometric visualisation map analysis to explore the publication outputs, trends, and hotspots for endocrine disruptors (EDMs) biosensor development in relation to mycotoxins. The analysis showed that the current literature primarily focuses on developing biosensors for detecting highly toxic and widely distributed mycotoxins in food, but there needs to be more focus on biosensors for detecting mycotoxins known as endocrine disruptors.
Article
Food Science & Technology
Yajie Zheng, Jianpeng Dou, Changpo Sun, Hujun Liu, Shipei Zhang, Wenfu Wu
Summary: This study investigated the efficiency of modified SA/ll-glucan/Mt microsphere adsorbents in removing DON and ZEN from CSL. The microspheres showed high adsorption capacity, and simple and rapid regeneration was achieved without affecting the removal efficiency of mycotoxins from CSL.
Article
Food Science & Technology
Carlos Adelantado, Jose Angel Salatti-Dorado, Carlos Herrera, Lourdes Arce, Maria Jose Cardador
Summary: High-performance-ion mobility spectrometry (HPIMS) was used for the first time in the analysis of olive oil samples, following a simple liquid-liquid extraction procedure. The developed methodology is user-friendly, fast, and inexpensive, complying with the principles of green chemistry. The proposed approach, combined with multivariate analysis, showed potential in classifying olive oil samples based on their organoleptic quality, with high accuracy rates.
Article
Food Science & Technology
Jia-Wen Wei, Jia-Rong He, Shi-Yi Chen, Yi-Han Guo, Xuan-Zhu Huo, Nuan Zheng, Shuo-Hui Cao, Yao-Qun Li
Summary: Polycyclic aromatic hydrocarbons (PAHs) are a major cause of human cancer. This study developed a quantitative analysis method for PAH4 using the back propagation neural network (BPNN) algorithm and constant wavelength synchronous fluorescence (CWSF) spectra as the data sets. The method can predict the concentrations of PAH4 in edible oil samples without preprocessing or pre-separation. It has been proven to be a powerful tool for the rapid detection of PAH4.
Article
Food Science & Technology
Liyun Chen, Xiumei Li, Jingyi Chen, Ranxun Lin, Yuhan Mai, Yuxin Lin, Guodong Wang, Zheng Chen, Wei Zhang, Jiang Wang, Cai Yuan, Longguang Jiang, Peng Xu, Mingdong Huang
Summary: Food spoilage is primarily caused by microbial contamination. Curcumin has shown moderate antimicrobial activity as a food preservative. This study formulates curcumin with zinc acetate to enhance its efficacy, resulting in Cur-Zn with increased antimicrobial activity against multiple food-contaminating bacteria and fungi. With light illumination, the antimicrobial activity of Cur-Zn is further enhanced due to the photodynamic inactivation (PDI) effect.
Article
Food Science & Technology
Marta Pilar Ortiz-Moriano, Gonzalo Machado-Schiaffino, Eva Garcia-Vazquez, Alba Ardura
Summary: Mislabelling in the global fisheries sector raises concerns about the identity, safety, and sustainability of seafood products. This study examined mislabelling in shrimps and prawns sold in the Spanish market and assessed potential risks of heavy metal ingestion.
Article
Food Science & Technology
Doyeon Kim, Jinwoo Kim, Minsik Kim
Summary: This study demonstrates the enhanced bactericidal activity of engineered lysins fused with amphipathic antimicrobial peptides against Gram-negative bacterium Cronobacter sakazakii. The fusion lysins are capable of disrupting bacterial cells and significantly reducing the risk of infection within a certain range of temperature and pH. Additionally, the study also shows the bactericidal effect of these fusion lysins against other Gram-negative pathogens.
Article
Food Science & Technology
Seul-Ki Park, Kyung-Jin Cho, Du-Min Jo, Young-Mog Kim
Summary: This study presents a new approach for detecting pathogenic Vibrio spp. in contaminated seafood, which combines PCR with a simplified DNA extraction process and pre-culturing step. The method has been validated and shown to be efficient, time-saving, and highly sensitive, offering a potential alternative for improving safety measures in the food industry.
Article
Food Science & Technology
Suwimol Surareungchai, Chaleeda Borompichaichartkul, Chitsiri Rachtanapun, Nutthachai Pongprasert, Pongphen Jitareerat, Varit Srilaong
Summary: This study builds upon previous research and focuses on risk assessment and analysis in the organic salad industry. The results show that previous risk matrix models can be adapted for various raw materials, and preventive control plans can be applied across different aspects of the production process.
Article
Food Science & Technology
Xuan Zhang, Changling Fang, Xiaoyi Lou, Feng Han, Siman Li, Yongfu Shi, Dongmei Huang
Summary: This study aimed to assess the levels of Biogenic amines (BAs) in four common food types in the Chinese market. It was found that cheese and seafood derived product showed higher levels of BAs, and the histamine level in mackerel and yellow rice wine exceeded the limits.
Article
Food Science & Technology
Martina Foschi, Alessandra Biancolillo, Federico Marini, Francesco Cosentino, Francesca Di Donato, Angelo Antonio D'Archivio
Summary: This study aimed to develop a potentially non-destructive and routine-compatible method for the geographical traceability of high-valued Italian chickpeas. By analyzing the spectral information of the outer and inner parts of the kernels, it was found that the spectral analysis of the outer part yielded accurate and reliable results, indicating the possibility of non-destructive authentication.
