Article
Chemistry, Applied
Di Wu, Ran Duan, Lan Tang, Xia Hu, Fang Geng, Qiaomei Sun, Yin Zhang, Hui Li
Summary: The interaction between lipase and quercetin 3-rhamnoside was studied using fluorescence spectroscopy, enzyme kinetics, and molecular dynamics simulation. Quercetin 3-rhamnoside showed a strong quenching effect on lipase fluorescence and inhibited the enzyme in a dose-dependent manner. Thermodynamic parameters indicated hydrogen bonding and van der Waals forces as the main driving forces for the interaction, causing structural perturbation in the lipase.
Article
Food Science & Technology
Hong Xu, Hang Gao, Feiyue Liu, Lingxiao Gong
Summary: This study found that red-skin extracts from lotus seeds can significantly improve obesity characteristics in high-fat-diet-fed mice by regulating lipoprotein lipase activity.
Article
Biochemistry & Molecular Biology
Bilal Ahmad, Emily P. Friar, Muhammad Sufyan Vohra, Nasar Khan, Christopher J. Serpell, Michelle D. Garrett, Jason Siau Ee Loo, Isabel Lim Fong, Eng Hwa Wong
Summary: This study investigates the anti-obesity effects of hydroxylated polymethoxyflavones (HPMFs) and explores their mechanism of action. It demonstrates that two HPMFs, 3' OH-TetMF and 4' OH-TetMF, have anti-obesity effects by inhibiting pancreatic lipase activity, suppressing adipogenesis-related gene expression, promoting lipid breakdown, and reducing adipokine secretion. These findings suggest that HPMFs have potential in the treatment of obesity.
CHEMICO-BIOLOGICAL INTERACTIONS
(2023)
Article
Multidisciplinary Sciences
Yusuke Moritoh, Shin-ichi Abe, Hiroki Akiyama, Akihiro Kobayashi, Ryokichi Koyama, Ryoma Hara, Shizuo Kasai, Masanori Watanabe
Summary: The enzymatic activity of IP6K is crucial for regulating phosphate levels in mammals, with its inhibition reducing cellular phosphate export and increasing intracellular ATP levels. Inhibition of IP6K lowers plasma phosphate levels and improves kidney functions in chronic kidney disease rats.
NATURE COMMUNICATIONS
(2021)
Review
Medicine, Research & Experimental
Yaosheng Xiao, Qun Ren, Longhuo Wu
Summary: Acteoside has various pharmacological activities and shows potential in drug development due to its modulation of multiple signaling pathways.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Pharmacology & Pharmacy
Jia Zhang, Kai Li, Hao-Ran Sun, Shao-Kun Sun, Ya-Ting Zhu, Yu-Ting Ge, Yu-Xuan Wu, Qin-Yao Zhou, Guan-Ting Li, Xiao-Ai Chang, Peng Sun, Ying Ding, Xiao Han
Summary: This study found that muparfostat (PI-88), an HS mimetic, can cause hyperlipidemia and aggravated hepatic steatosis in obese mouse models and cultured murine hepatocytes. In in vivo tests, muparfostat reduces the degradation of circulating triglycerides by competitively binding to HS on vascular endothelial cell surfaces, leading to the uptake of fatty acids into vascular endothelial cells and causing hyperlipidemia. This hyperlipidemia exacerbates hepatic steatosis and causes liver injury in muparfostat-treated obese mice. Therefore, this study reveals that the binding activity of HS mimetics to lipoprotein lipase should be investigated as an additional pharmacological effect during heparanase inhibitor drug discovery, and provides novel evidence for an increased risk of drug-induced liver injury in obese individuals.
BRITISH JOURNAL OF PHARMACOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Marek Wilhelm, Lenka Rossmeislova, Michaela Siklova
Summary: This article reviews the characteristics of lipogenic and lipolytic enzymes and the methods available for measuring their activity. It evaluates the applicability of these methods to different biological samples and discusses the advantages and disadvantages of these methods in vivo, ex vivo, and in vitro.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Yuanyuan Wu, Mengnan Zeng, Ruiqi Xu, Beibei Zhang, Shengchao Wang, Benke Li, Yuxuan Kan, Bing Cao, Xiaoke Zheng, Weisheng Feng
Summary: Acteoside can inhibit melanoma growth, alleviate inflammation levels in mice, and regulate ROS and apoptosis levels in the spleen. By regulating the ERA-Ras/Raf1-STAT3 signaling axis, acteoside promotes tumor cell apoptosis, thus inhibiting the occurrence and development of melanoma.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2021)
Article
Biochemistry & Molecular Biology
Eisuke Kato, Ai Tsuruma, Ayaka Amishima, Hiroshi Satoh
Summary: Young barley leaf extract (YB) has pancreatic lipase inhibitory activity, which reduces lipid absorption and exhibits antiobesity effects. Fructose-bisphosphate aldolase in YB serves as a proteinous lipase inhibitor, showing characteristic inhibition at a low concentration of 16 ng/mL.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2021)
Review
Chemistry, Analytical
Silvia Rocha, Ana T. Rufino, Marisa Freitas, Artur M. S. Silva, Felix Carvalho, Eduarda Fernandes
Summary: Obesity is a concerning epidemic, and pancreatic lipase has been explored as an alternative for weight loss by reducing fat absorption. This review aims to understand and summarize the methodologies and experimental conditions used to evaluate pancreatic lipase catalytic activity.
CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Na Li, Xi Liu, Jing Zhang, Yan-Zhi Lang, Lu Lu, Jia Mi, You-Long Cao, Ya-Mei Yan, Lin-Wu Ran
Summary: This study demonstrates that anthocyanins from Lycium ruthenicum fruits have preventive effects in high-fat-diet induced obese mice. Anthocyanins regulate the diversity of gut microbiota, decrease the ratio of Firmicutes to Bacteroidota, increase the abundance of Akkermansia, and reduce the abundance of Faecalibaculum to prevent obesity.
Article
Chemistry, Applied
Tian-Ci Yan, Zi-Xuan Yue, Yu-Xin Gu, Hui Zheng, Jun Cao
Summary: An efficient and environmentally friendly method for online screening of lipase inhibitors using capillary electrophoresis was established, with optimized conditions and molecular docking technology validating the inhibitory activity of citrus flavonoids on lipase. The extract of Tangerine Pith showed the highest inhibitory activity, and tangeretin, nobiletin, hesperetin, and naringenin were identified as potential inhibitors of lipase through hydrogen bond formation. This method can be used effectively for preliminary screening of lipase inhibitors from citrus fruits.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2022)
Article
Chemistry, Medicinal
Zhao Zhuoyue, Wijitrapha Ruangaram, Eisuke Kato
Summary: Acacia concinna is a medicinal plant from Southeast Asia with potential anti-obesogenic compounds in its pods. Saponins isolated from the pods showed strong inhibition of pancreatic lipase activity, reduced lipid accumulation, and enhanced lipolysis in adipocytes, suggesting their potential for treating and preventing obesity in the future.
JOURNAL OF NATURAL MEDICINES
(2021)
Article
Chemistry, Analytical
Jia Liu, Hai-Xia Zhang, Yan-Ping Shi
Summary: This study successfully improved the stability and reusability of lipase by immobilizing it, and identified Crataegus pinnatifida Bunge as a potential anti-obesity drug due to its significant lipase inhibitory activity. The established method not only developed a platform for discovering novel anti-obesity drugs from traditional herbal medicines, but also laid a solid foundation for exploring the undiscovered medicinal value of these herbs.
Article
Biochemistry & Molecular Biology
Jong-Yeon Kim, You-Suk Lee, Eun-Jung Park, Hae-Jeung Lee
Summary: This study found that honeysuckle berry has inhibitory effects on lipase activity and can modulate gut microbiota composition, leading to reduced body fat. It suggests that honeysuckle berry could be a potential agent for treating metabolic diseases.
Article
Chemistry, Applied
M. Graca Dias, Elsa Vasco, Francisco Ravasco, Lufsa Oliveira
Summary: This study estimated the vitamin D intake of "adults" and "elderly" populations in Portugal using the TDS methodology. The results showed that the majority of people had inadequate vitamin D intake, well below the Dietary Reference Values.
Article
Chemistry, Applied
Yanan Wang, Jiachen Shi, Yong-Jiang Xu, Chin-Ping Tan, Yuanfa Liu
Summary: This study investigates the variations in lipid digestion profiles among individuals of different ages using in vitro digestion models. The findings suggest that adults have a more comprehensive lipid digestion compared to infants, and infants tend to release shorter chain length and more saturated free fatty acids during digestion. Additionally, the particle sizes in the stomach of the elderly were consistently larger. This study enhances our understanding of how lipids with different degrees of unsaturation undergo digestion in diverse age groups.
Article
Chemistry, Applied
Hyunjong Yu, Huisu Kim, Pahn-Shick Chang
Summary: Chitosan-pectin emulsion-filled hydrogel (EFH) was developed to enhance the bioaccessibility of lipophilic bioactive compounds through intestinal delivery. The EFH, prepared without crosslinking agents, demonstrated improved mechanical strength and compactness with higher pectin concentration. It retained the emulsion at pH 2.0 and released it at pH 7.4, resulting in enhanced release of free fatty acids and improved bioaccessibility of curcumin.
Article
Chemistry, Applied
Tongze Zhang, Siqi Hong, Jia-Rong Zhang, Pin-He Liu, Siyi Li, Zixian Wen, Jianwei Xiao, Guirong Zhang, Olivier Habimana, Nagendra P. Shah, Zhongquan Sui, Harold Corke
Summary: Lactic acid fermentation significantly affects the morphology and physicochemical properties of proso millet starch, including the formation of surface indentations and small pores, decrease in gelatinization temperatures, and changes in hardness and adhesiveness.
Article
Chemistry, Applied
Liqin Kong, Feng Hong, Peng Luan, Yiping Chen, Yaoze Feng, Ming Zhu
Summary: This study presents a novel impedance biosensor using composite nanomaterials and T1R1 as a signal probe, which can competitively and ultra-sensitively detect umami intensity. The biosensor exhibits exceptional analytical performance and is suitable for food flavor evaluation.
Article
Chemistry, Applied
Kunying Lv, Yixin Yang, Qilong Li, Ran Chen, Liang Deng, Yiwei Zhang, Ning Jiang
Summary: Horse's milk, with its high nutritional value and low allergenic proteins, could be a substitute for cow's milk in infant consumption. A proteomic method was used to identify and compare milk fat globule membrane (MFGM) and whey proteins from different horse breeds. The study found differences in protein composition and functionality, which could support the development of formulas more suitable for human infants.
Article
Chemistry, Applied
Enrique Jacobo Diaz-Montana, Helene Brignot, Ramon Aparicio-Ruiz, Thierry Thomas- Danguin, Maria Teresa Morales
Summary: Sensory perception of virgin olive oil is influenced by phenols and volatiles, which are affected by the composition of the oil and biological factors. This study investigated the effect of saliva and phenols on the release of volatiles, and found that the presence of phenols decreased the release of saturated volatiles.
Article
Chemistry, Applied
Wei Zhou, Rui Zhang, Zhen Cai, Fangfang Wu, Yong Hu, Chao Huang, Kun Hu, Yun Chen
Summary: Environmentally friendly and outstanding pH-responsive cationic starch nanoparticles (CSNP) were prepared from pH-sensitive starch. CSNP exhibited nanosize and regular sphere, highly free-flowing molecular chains, and demonstrated excellent pH responsiveness through multiple emulsion/demulsification transitions.
Article
Chemistry, Applied
Andrea Koo, Vinayak Ghate, Weibiao Zhou
Summary: This study suggests that direct seeding may negatively affect the nutritional quality of crops, causing a decrease in ascorbic acid, vitamin K, and total glucosinolate content.
Article
Chemistry, Applied
Tonghao Du, Yazhou Xu, Xiaoyan Xu, Shijin Xiong, Linli Zhang, Biao Dong, Jinqing Huang, Tao Huang, Muyan Xiao, Tao Xiong, Mingyong Xie
Summary: This study successfully improved the ACE inhibitory activity of black sesame seeds by fermenting them with Lactobacillus Plantarum NCU116 and hydrolyzing them using acid protease. The RF-PSO model was used to predict the ACE inhibitory activity during the hydrolysis process. Eight peptides with ACE inhibitory activity were identified from fermented black sesame seed hydrolysates after separation and screening.
Article
Chemistry, Applied
Yao Liu, Songyi Lin, Kexin Liu, Shan Wang, Qiaozhen Liu, Na Sun
Summary: This study analyzed the structural changes of shrimp proteins during digestion, predicted the immunodominant epitopes, and validated their allergenicity. The results showed that shrimp proteins were degraded into peptides during digestion, but still carried IgE epitopes that trigger allergic reactions.
Article
Chemistry, Applied
Tiantian Fu, Hongwei Cao, Yu Zhang, Xiao Guan
Summary: This study investigates the impact of milling on the active components in rice, with a focus on the stability and bioaccessibility of phenols, VB1, and alpha-GABA during cooking and digestion. The findings show that milling exacerbates the instability of gamma-GABA during cooking and VB1 during digestion, and it affects the bioaccessibility of these active compounds.
Article
Chemistry, Applied
Zhihao Yang, Yanru Hou, Min Zhang, Puxin Hou, Chang Liu, Lu Dou, Xiaoyu Chen, Lihua Zhao, Lin Su, Ye Jin
Summary: This study investigated the molecular mechanism of feeding regimes on lamb flavor by using TMT labeling combined with MS. The results showed that pasture-fed groups had higher levels of amino acids and volatile flavor substances compared to concentrate-fed groups. Additionally, several differentially abundant proteins associated with lamb flavor were identified.
Article
Chemistry, Applied
Zixuan Xie, De Zhang, Junyu Zhu, Qianqian Luo, Jun Liu, Jingtao Zhou, Xiaoyong Wang, Yuqiong Chen, Zhi Yu, Dejiang Ni
Summary: This study investigated the acidification of aroma-enhanced black tea during storage. Analysis of non-volatile substances and organic acids using UPLC-Q-TOF/MS and HPLC revealed a decrease in soluble sugars and amino acids, while an increase in organic acids such as oxalic acid, malic acid, and quinic acid. In vitro experiments further demonstrated that the acidification is a result of the decomposition of sugars and amino acids by heating, as well as the oxidation of aromatic aldehydes. Additionally, the study showed that the taste composition of tea infusion is altered, with reduced amino acids, catechins, soluble sugars, and flavonoids. This research provides a theoretical basis for improving the quality of black tea.
Article
Chemistry, Applied
Lin Wang, Falai Ma, Zihan Li, Yan Zhang
Summary: This study developed time-temperature integrators based on amyloglucosidase@Cu3(PO4)2 nanoflowers for monitoring the freshness of chilled pork. The results showed that the integrators were highly reliable and accurate in predicting the quality of chilled pork.