期刊
FOOD CHEMISTRY
卷 152, 期 -, 页码 66-74出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2013.11.125
关键词
Glucosinolates Broccoli leaves; LC-DAD-ESI-MS; Microwave extraction; Box-Behnken design
资金
- Junta de Castilla y Leon
- Spanish Government [RTA2012-00076-C02-02]
- Spanish Ministry Educacion, Cultura y Deporte
A new method has been developed and validated to determine twelve intact glucosinolates (glucoiberin, GIB; glucoraphanin, GRA; glucoerucin GER; gluconapin, GNA; glucotropaeolin, GTL; glucobrassicin, GBC; gluconasturtiin, GST; glucoalyssin, ALY; 4-hydroxyglucobrassicin, 4-OH; 4-metoxyglucobrassicin, 4ME; neoglucobrassicin, NEO; sinigrin, SIN) in broccoli leaves using liquid chromatography (LC) coupled to diode array (DAD) and electrospray ionization mass spectrometry (ESI-MS) detection. An extraction procedure has also been proposed and optimized by means of statistical analysis (the Box-Behnken design and analysis of variance); this is based on the deactivation of myrosinase using a microwave and heated water. Low limits of detection and quantification were obtained, ranging from 10 to 72 mu g/g with DAD and 0.01 to 0.23 mu g/g with ESI-MS, and the resulting recovery values ranged from 87% to 106% in all cases. Finally, glucosinolates were analyzed in broccoli leaf samples from six different cultivars (Ramoso calabrese Parthenon, Marathon, Nubia, Naxos and Viola). (c) 2013 Elsevier Ltd. All rights reserved.
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