期刊
FOOD CHEMISTRY
卷 139, 期 1-4, 页码 872-877出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2013.01.116
关键词
Dialyzability; Total selenium; Selenium species; Seafood; Characterization
资金
- Xunta de Galicia (Programa de Consolidacion y Estructuracion de Unidades de Investigacion Competitivas
- Ministerio de Ciencia y Tecnologia [AGL-2006-11034]
- Programa Sectorial de investiagacion aplicada
- Peme 1+D e 1+D Suma do Plan Galego de Investigacion
- Desenvolvemento e Innovacion Tecnoloxica (Incite) [09MDS038103PR]
In vitro bioavailability of total selenium and selenium species from different raw seafood has been assessed by using a simulated gastric and intestinal digestion/dialysis method. Inductively coupled plasma-mass spectrometry (ICP-MS) was used to assess total selenium contents after a microwave assisted acid digestion, and also to quantify total selenium in the dialyzable and non-dialyzable fractions. Selenium speciation in the dialyzates was assessed by high performance liquid chromatography (HPLC) coupled with ICP-MS detection. Major Se species (selenium methionine and oxidized selenium methionine) from dialyzate were identified and characterized by HPLC coupled to mass spectrometry (HPLC-MS). Selenocystine was detected at low concentrations while Se-(Methyl)selenocysteine and inorganic selenium species (selenite and selenate) were not detected in the dialyzate. Low bioavailability percentages for total selenium (6.69 +/- 3.39 and 5.45 +/- 2.44% for fish and mollusk samples, respectively) were obtained. Similar bioavailability percentages was achieved for total selenium as a sum of selenium species (selenocystine plus oxidized selenium methionine and selenium methionine, mainly). HPLC-MS data confirmed SeMet oxidation during the in vitro procedure. (C) 2013 Elsevier Ltd. All rights reserved.
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