4.7 Article

Monitoring of Escherichia coli O157:H7 in food samples using lectin based surface plasmon resonance biosensor

期刊

FOOD CHEMISTRY
卷 136, 期 3-4, 页码 1303-1308

出版社

ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2012.09.069

关键词

Lectin; Surface plasmon resonance biosensor; Escherichia coli O157:H7; Food sample

资金

  1. National Natural Science Foundation of China [20907041]
  2. Zhejiang Provincial Natural Science Foundation of China [Y2080914]

向作者/读者索取更多资源

A novel surface plasmon resonance (SPR) biosensor using lectin as bioreceptor was developed for the rapid detection of Escherichia coli (E. coli) O157:H7. The selective interaction of lectins with carbohydrate components from bacterial cells surface was used as the recognition principle for the detection. Five types of lectins from Triticum vulgaris, Canavailia ensiformis, Ulex europaeus, Arachis hypogaea, and Maackia amurensis, were employed to evaluate the selectivity of the approach for binding E. coli O157:H7 effectively. A detection limit of 3 x 10(3) cfu mL(-1) was obtained for determination of E. coli O157:H7 when used the lectin from T. vulgaris as the binding molecule. Furthermore, the proposed biosensor was used to detect E. coli O157:H7 in real food samples. Results showed that the lectin based SPR biosensor was sensitive, reliable and effective for detection of E. coli 0157:H7, which hold a great promise in food safety analysis. (C) 2012 Elsevier Ltd. All rights reserved.

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