期刊
FOOD CHEMISTRY
卷 141, 期 2, 页码 1287-1294出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2013.03.088
关键词
Sea cucumber; Body wall; Autolysis; Noncollagenous proteins; Cysteine proteases
资金
- National Natural Science Foundation of China [31000754, 30901124]
Proteolysis of noncollagenous proteins in sea cucumber, Stichopus Japonicus, body wall (sjBW) was investigated. The proteins removed from sjBW by SDS and urea extraction were mainly noncollagenous proteins with molecular weights about 200 kDa (Band I) and 44 kDa (Band II), respectively. Band I and Band II were identified as major yolk protein (MYP) and actin, respectively, from holothurian species by liquid chromatography-mass spectrometry (LC-MS/MS) with significant scores. Based on TCA-soluble oligopeptide assay, the optimum proteolysis condition of noncollagenous proteins was at 46.3 degrees C and pH 6.1, by response surface methodology. The proteolysis of MYP, and actin, was partially inhibited by cysteine protease inhibitors, including Trans-epoxysuccinyl-L-leucyl-amido (4-guanidino) butane (E-64), iodoacetic acid, antipain and whey protein concentrate. These results suggest that cysteine proteases are partially involved in the proteolysis of noncollagenous proteins in body wall of sea cucumber, S. japonicus. Crown Copyright (C) 2013 Published by Elsevier Ltd. All rights reserved.
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