Article
Biochemical Research Methods
Mihai Victor Curtasu, Natalja P. Norskov
Summary: The present work aims to develop a high-yield extraction procedure combined with a robust, sensitive, and fast LC-MS/MS method for quantifying flavonoids and salicylic acid in Willow bark. The research investigates the effect of freeze and oven-drying procedures and five extraction solvents on the yield of individual flavonoids and salicylic acid. The freeze-drying procedure is the best for preserving monomeric and polymeric flavan-3-ols, while the best extraction solvent is methanol acidified with 1% hydrochloric acid.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Chemistry, Applied
Ajay Anand, Anusha Komati, Kumar Katragunta, Hussain Shaik, Narendra Kumar Nagendla, Madhusudana Kuncha, Mohana Krishna Reddy Mudiam, Katragadda Suresh Babu, Ashok Kumar Tiwari
Summary: Boiled rhizome of Nymphaea nouchali shows significant antihyperglycemic and antioxidative stress potentials, making it a promising food material for managing hyperglycemia and oxidative stress.
Article
Medicine, Research & Experimental
Arafa Hamed, Ridha Ben Said, Mohamed Ali Ben Aissa, Ibrahim Bayoumi Abdel-Farid, Bogdan Kontek, Mariusz Kowalczyk, Wieslaw Oleszek, Anna Stochmal, Ivona Kowalska, Beata Olas
Summary: This study tentatively elucidated the structure of polyoxypregnane glycosides from Caralluma quadrangula and explored their fragmentation pathways. The analysis provided useful information for phytochemistry analysis, which can reduce material consuming and time.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Energy & Fuels
Nushrat Yeasmen, Valerie Orsat
Summary: A maceration process was optimized to extract phenolic compounds from sugar maple leaves. High-speed homogenization was found to be an effective pre-treatment. The study identified the optimal conditions for extraction and characterized the extract. LC-ESI-Q-TOF-MS/MS analysis identified 81 phenolic compounds and proposed a gallotannins pathway.
BIOMASS CONVERSION AND BIOREFINERY
(2022)
Article
Plant Sciences
Jiameng Qu, Fan Ke, Ziru Liu, Xiao Yang, Xianzhe Li, Huarong Xu, Qing Li, Kaishun Bi
Summary: This study comprehensively demonstrates the multi-target mechanisms of dandelion against TNBC using network pharmacology, molecular pharmacology, and metabolomics approaches. The findings indicate that dandelion can treat TNBC by regulating cell cycle and metabolism-related biological processes. These findings are of great importance for further mechanism investigations and the development of highly effective dandelion-based treatments for TNBC.
Article
Food Science & Technology
Jing Wan, Peng He, Yuanshan Chen, Qiujin Zhu
Summary: A practical, efficient, and rapid method for quantifying 19 pyrethroids in tea and orange samples was developed. Out of 262 tea samples, 11 pyrethroids were detected positive with concentrations below maximum residue limits. No pyrethroid was found in 209 peeled orange samples.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Cell Biology
Chandranayaka Siddaiah, Anil Kumar BM, Saligrama Adavigowda Deepak, Syed Salman Lateef, Saurabh Nagpal, Kanchugarakoppal S. Rangappa, Chakrabhavi D. Mohan, Shobith Rangappa, Madan S. Kumar, Minaxi Sharma, Vijai Kumar Gupta
Summary: This study focused on metabolite profiling and identification from the bark of Alangium salviifolium by using organic and aqueous solvents. Eighty-one secondary metabolites with therapeutic potential were identified, showcasing the therapeutic value of this plant.
Review
Chemistry, Analytical
Hugo M. Santos, Luis B. Carvalho, Carlos Lodeiro, Goncalo Martins, Ines L. Gomes, Wilson D. T. Antunes, Vanessa Correia, Maria M. Almeida-Santos, Helena Rebelo-de-Andrade, Antonio P. A. Matos, J. L. Capelo
Summary: This work presents a tutorial on the capabilities of bioanalytical mass spectrometry in detecting and differentiating viruses at the peptide level, as well as providing diagnosis and prognosis for infected patients. It offers insights into the possibilities of current high-resolution mass spectrometry technology and bioinformatics.
MICROCHEMICAL JOURNAL
(2023)
Review
Food Science & Technology
Minke Yang, Juan Li, Chengying Zhao, Hang Xiao, Xiang Fang, Jinkai Zheng
Summary: Flavonoids have gained increasing attention for their various health-promoting effects. Liquid chromatography-quadrupole time-of-flight tandem mass spectrometry (LC-Q-TOF-MS/MS) has become a widely used technique for detecting flavonoids, providing efficient separation and structural information. This review comprehensively summarizes the principles and methodologies of LC-Q-TOF-MS/MS for detecting flavonoids in food, including qualitative and quantitative determination. It also discusses recent applications in agricultural products, food processing, and in vivo metabolism, as well as suggesting technological improvements and potential applications.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Biochemistry & Molecular Biology
Guo-Ming Dong, Hang Yu, Li-Bin Pan, Shu-Rong Ma, Hui Xu, Zheng-Wei Zhang, Pei Han, Jie Fu, Xin-Yu Yang, Adili Keranmu, Hai-Tao Niu, Jian-Dong Jiang, Yan Wang
Summary: The study revealed that gut microbiota plays a significant role in the metabolism of timosaponin BII, resulting in the production of various metabolites, including three newly identified structures in the gut microbiota. The metabolic behavior of timosaponin BII in gut microbiota system was found to be unique compared to rat liver homogenate and a rat liver microsome incubation system.
Article
Food Science & Technology
Nannan Li, Shuqin Li, Qirou Wang, Shuyu Yang, Yinuo Hou, Yan Gao, Xiangyang Zhang, Min Zhang, Haixia Chen
Summary: This study applied MALDI-TOF MSI and QTOF LC-MS/MS techniques to investigate the spatial distribution changes of low molecular weight compounds in garlic tissue during Laba garlic processing. It was found that some bioactive compounds were transformed or leached out during the processing, while new compounds related to pigments emerged. This study provides a basis for understanding the spatial distributions and changes of compounds in garlic tissue during Laba garlic processing, which may affect the bioactivities of garlic.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Biochemical Research Methods
Daseul Jeong, Sanggil Choe, Sooyeun Lee, Kang Min Kim, Jaesung Pyo
Summary: The metabolites of five major illegal anti-obesity drugs were analyzed for the first time, providing a tool for toxicological detection and metabolite identification in forensic cases. The study revealed that these drugs were metabolized into multiple constituents in in vivo and in vitro models.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Food Science & Technology
Sanja Vlaisavljevic, Milena Raseta, Sanja Berezni, Sabina Passamonti, Federica Tramer
Summary: The study found that Arame algae extract has the highest scavenging activity, and the antioxidant potential of all extracts is correlated with total phenol content, rather than total carotenoids content. Although biomolecules in the algae extracts showed in vitro antioxidant activity, they did not exhibit a significant effect on PC3 cells.
INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION
(2021)
Article
Plant Sciences
Ivana Carev, Cengiz Sarikurkcu
Summary: The study demonstrated the significant antioxidant activity of the endemic Stachys species S. cretica ssp. anatolica in Turkey. The extracts of this plant are rich in polyphenols, showing strong activity against antioxidant enzymes and potential therapeutic effects for skin disorders and diabetes.
Article
Agriculture, Multidisciplinary
Aipeng Hu, Fang Wei, Fenghong Huang, Ya Xie, Bangfu Wu, Xin Lv, Hong Chen
Summary: An ultrahigh-performance liquid chromatography coupled with mass spectrometry method was established for comprehensive lipidomic profiling of oilseeds, revealing significant variations in lipid composition and content among soybean, sesame, peanut, and rapeseed. The study provided a theoretical basis for the selection and breeding of oilseed varieties as well as processing of oilseed for the edible oil industry.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Min-Jeong Kim, Eun-Sang Hwang, Kwan Joong Kim, Sungho Maeng, Ho Jin Heo, Ji-Ho Park, Dae-Ok Kim
Summary: This study found that EGCG can alleviate scopolamine-induced learning and memory impairments and improve brain function. This may be achieved through mechanisms such as improving cholinergic function and reducing oxidative stress.
Article
Chemistry, Applied
Mi-Seon Kim, Young Sung Jung, Davin Jang, Chi Heung Cho, Sang-Hoon Lee, Nam Soo Han, Dae-Ok Kim
Summary: This study evaluated the antioxidant capacity, bioaccessibility, and bioavailability of 12 soy isoflavones (SIs). The results showed that SIs were stable under gastric digestion conditions but unstable under small intestinal digestion conditions for acetyl and malonyl conjugates. SI aglycones had higher permeability and cellular accumulation than their glucosides. A small amount of intact SI conjugates were found in the cell and in the basolateral side of each Transwell.
Article
Food Science & Technology
Briana M. Nosal, Junichi R. Sakaki, Dae-Ok Kim, Ock K. Chun
Summary: The brewing methods and types of coffee can affect the antioxidant capacity of coffee consumers, with capsule coffee showing higher total phenolic content and antioxidant capacity.
FOOD SCIENCE AND BIOTECHNOLOGY
(2022)
Review
Food Science & Technology
Ahmad Rois Mansur, Sang Gil Lee, Bong-Han Lee, Sang Gyu Han, Sung-Won Choi, Won-Jae Song, Tae Gyu Nam
Summary: Phenolic compounds in common buckwheat sprouts have been shown to possess various health benefits, including anti-inflammatory, antioxidant, anti-proliferative, and immunomodulatory effects. HPLC coupled with a UV-visible detector and/or MS is the most commonly used analytical method for determining the phenolic profile.
FOOD SCIENCE AND BIOTECHNOLOGY
(2022)
Article
Food Science & Technology
Sun Hye Hwang, JaeHwan Lee, Tae Gyu Nam, Minseon Koo, Yong Sun Cho
Summary: The study observed significant changes in the physicochemical properties and microorganism communities of aged beef during refrigerated storage, including exceeded VBN values, fluctuations in various microbial populations, and contributions of volatile substances and amino acids to high VBN levels.
FOOD SCIENCE & NUTRITION
(2022)
Article
Integrative & Complementary Medicine
Eun-Sang Hwang, Ga-Young Choi, Kwan Joong Kim, Min-Jeong Kim, Seok Lee, Jin-Won Lee, Dae-Ok Kim, Ji-Ho Park
Summary: Vitamin C (VC) can lower blood pressure in spontaneously hypertensive rats (SHRs) by directly targeting ACE I production and may improve endothelial function, with the frequency of administration affecting its effectiveness.
EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
(2022)
Article
Food Science & Technology
Young Sung Jung, Nho-Eul Song, Ji Yeon Choi, Sun Hye Hwang, Minseon Koo, Tae Gyu Nam
Summary: The study evaluated the effects of different sorbents on removing pigments from kale extracts, and found that a sorbent combination based on Z-Sep+ effectively improved pigment removal capacity while maintaining high pesticide recovery rates and low matrix effects.
FOOD SCIENCE AND BIOTECHNOLOGY
(2022)
Article
Agriculture, Multidisciplinary
Young Sung Jung, Hyoung-Geun Kim, Seon Min Oh, Dae Young Lee, Cheon-Seok Park, Dae-Ok Kim, Nam-In Baek
Summary: Using amylosucrase from Deinococcus geothermalis, alpha-linked glucosylation of Soybean isoflavone aglycones (SIAs) was achieved, resulting in 13 alpha-linked glucosyl SIAs with confirmed colors in solution. The structures of the compounds were identified using mass spectrometry and multidimensional nuclear magnetic resonance spectroscopy. The study suggests a strategy for the synthesis of bioactive compounds with enhanced water solubility through alpha-linked glucosylation, with the products attached to the C-4 ' of SIAs found to be more water-soluble than those attached to the C-7 and/or beta-linkages.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Kwan Joong Kim, Eun-Sang Hwang, Min-Jeong Kim, Chan-Su Rha, Myoung Chong Song, Sungho Maeng, Ji-Ho Park, Dae-Ok Kim
Summary: The study demonstrated the cognitive-enhancing effect of Pinus densiflora Sieb. et Zucc. bark extract (PBE). PBE improved cognitive impairment in rats through its antioxidant properties and enhancement of long-term potentiation (LTP) induction in organotypic hippocampal slices.
Article
Chemistry, Applied
Chan-Su Rha, Eun Kyu Jang, Jong Suk Lee, Ji-Sung Kim, Min-Ji Ko, Sol Lim, Gun Hwan Park, Dae-Ok Kim
Summary: A study was conducted on the chemical constituents of soybean leaves and their correlations across phenotypes, growing periods, and cultivation areas using targeted metabolomics and machine learning models. The distribution of isoflavones and flavonols was found to be influenced by the growing period and cultivation area, and the neural network model showed the best predictive capacity for the differences in soybean leaves.
Article
Food Science & Technology
Ga-Young Choi, Kwan Joong Kim, Ho-Sub Park, Eun-Sang Hwang, Jae-Min Cho, Hyun-Bum Kim, Dae-Ok Kim, Ji-Ho Park
Summary: The study investigated the potential anti-atopic dermatitis (AD) effects of a herbal combination of Lithospermum erythrorhizon (LE), Houttuynia cordata (HC), and Spirodela polyrhiza (SP). Through ultra-high-pressure liquid chromatography (UHPLC)-Orbitrap mass spectrometer (MS), a total of 42 compounds were identified in LE, HC, SP, and the combined herb extract of LE, HC, and SP (LHS). The LHS showed increased concentration of flavonoid glycosides compared to individual extracts, and effectively inhibited the key characteristics of AD such as epidermal thickness increase, mast cell proliferation, and immunoglobulin E release.
FOOD SCIENCE AND BIOTECHNOLOGY
(2023)
Review
Food Science & Technology
Young Sung Jung, Hyunbin Seong, Nam Soo Han, Dae-Ok Kim
Summary: Soybean is an important crop and a significant dietary source of isoflavones. Understanding the bioaccessibility and bioavailability of soy isoflavones is crucial for comprehending their bioactivities. This review evaluates reports using in vitro methods to study the bioavailability of soy isoflavones and discusses how their molecular structures influence their bioactivities.
FOOD REVIEWS INTERNATIONAL
(2023)
Article
Food Science & Technology
Eun-Ha Park, Inil Lee, Gi-Cheol Park, Seung-Ju Lee, Kwan Joong Kim, Jisuk Yun, Dae-Ok Kim
Summary: Canola meal, a by-product of canola oil processing, has antioxidant and skin anti-aging effects. This is due to the bioactive compounds found in canola meal, particularly the antioxidative phenolics present in canola meal extract (CME).
FOOD SCIENCE AND BIOTECHNOLOGY
(2023)
Article
Agriculture, Multidisciplinary
Young Sung Jung, Hyoung-Geun Kim, Seon Min Oh, Dae Young Lee, Cheon-Seok Park, Dae-Ok Kim, Nam-In Baek
Summary: Soybean isoflavone aglycones (SIAs) have low water solubility in the human body. By glycosylation, SIAs can obtain structural diversity and change their physicochemical properties, including water solubility. The alpha-linked glucosylation of SIAs was achieved using amylosucrase from Deinococcus geothermalis. 13 alpha-linked glucosyl SIAs were obtained and their structures were identified by mass spectrometry and multidimensional nuclear magnetic resonance spectroscopy. The glucosylated SIAs attached to C-4' were found to be more water-soluble than those attached to C-7 and/or beta-linkages.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Min Young Um, Minseok Yoon, Minji Kim, Jonghoon Jung, Seonghui Kim, Dae-Ok Kim, Suengmok Cho
Summary: This study aimed to investigate the effects and underlying mechanisms of curcuminoids and their constituents on the sleep-wake cycle of mice. The results showed that curcuminoids reduced sleep latency and increased sleep duration by interacting with H1R. These findings suggest the potential importance of curcuminoids in treating insomnia.
Article
Chemistry, Applied
M. Graca Dias, Elsa Vasco, Francisco Ravasco, Lufsa Oliveira
Summary: This study estimated the vitamin D intake of "adults" and "elderly" populations in Portugal using the TDS methodology. The results showed that the majority of people had inadequate vitamin D intake, well below the Dietary Reference Values.
Article
Chemistry, Applied
Yanan Wang, Jiachen Shi, Yong-Jiang Xu, Chin-Ping Tan, Yuanfa Liu
Summary: This study investigates the variations in lipid digestion profiles among individuals of different ages using in vitro digestion models. The findings suggest that adults have a more comprehensive lipid digestion compared to infants, and infants tend to release shorter chain length and more saturated free fatty acids during digestion. Additionally, the particle sizes in the stomach of the elderly were consistently larger. This study enhances our understanding of how lipids with different degrees of unsaturation undergo digestion in diverse age groups.
Article
Chemistry, Applied
Hyunjong Yu, Huisu Kim, Pahn-Shick Chang
Summary: Chitosan-pectin emulsion-filled hydrogel (EFH) was developed to enhance the bioaccessibility of lipophilic bioactive compounds through intestinal delivery. The EFH, prepared without crosslinking agents, demonstrated improved mechanical strength and compactness with higher pectin concentration. It retained the emulsion at pH 2.0 and released it at pH 7.4, resulting in enhanced release of free fatty acids and improved bioaccessibility of curcumin.
Article
Chemistry, Applied
Tongze Zhang, Siqi Hong, Jia-Rong Zhang, Pin-He Liu, Siyi Li, Zixian Wen, Jianwei Xiao, Guirong Zhang, Olivier Habimana, Nagendra P. Shah, Zhongquan Sui, Harold Corke
Summary: Lactic acid fermentation significantly affects the morphology and physicochemical properties of proso millet starch, including the formation of surface indentations and small pores, decrease in gelatinization temperatures, and changes in hardness and adhesiveness.
Article
Chemistry, Applied
Liqin Kong, Feng Hong, Peng Luan, Yiping Chen, Yaoze Feng, Ming Zhu
Summary: This study presents a novel impedance biosensor using composite nanomaterials and T1R1 as a signal probe, which can competitively and ultra-sensitively detect umami intensity. The biosensor exhibits exceptional analytical performance and is suitable for food flavor evaluation.
Article
Chemistry, Applied
Kunying Lv, Yixin Yang, Qilong Li, Ran Chen, Liang Deng, Yiwei Zhang, Ning Jiang
Summary: Horse's milk, with its high nutritional value and low allergenic proteins, could be a substitute for cow's milk in infant consumption. A proteomic method was used to identify and compare milk fat globule membrane (MFGM) and whey proteins from different horse breeds. The study found differences in protein composition and functionality, which could support the development of formulas more suitable for human infants.
Article
Chemistry, Applied
Enrique Jacobo Diaz-Montana, Helene Brignot, Ramon Aparicio-Ruiz, Thierry Thomas- Danguin, Maria Teresa Morales
Summary: Sensory perception of virgin olive oil is influenced by phenols and volatiles, which are affected by the composition of the oil and biological factors. This study investigated the effect of saliva and phenols on the release of volatiles, and found that the presence of phenols decreased the release of saturated volatiles.
Article
Chemistry, Applied
Wei Zhou, Rui Zhang, Zhen Cai, Fangfang Wu, Yong Hu, Chao Huang, Kun Hu, Yun Chen
Summary: Environmentally friendly and outstanding pH-responsive cationic starch nanoparticles (CSNP) were prepared from pH-sensitive starch. CSNP exhibited nanosize and regular sphere, highly free-flowing molecular chains, and demonstrated excellent pH responsiveness through multiple emulsion/demulsification transitions.
Article
Chemistry, Applied
Andrea Koo, Vinayak Ghate, Weibiao Zhou
Summary: This study suggests that direct seeding may negatively affect the nutritional quality of crops, causing a decrease in ascorbic acid, vitamin K, and total glucosinolate content.
Article
Chemistry, Applied
Tonghao Du, Yazhou Xu, Xiaoyan Xu, Shijin Xiong, Linli Zhang, Biao Dong, Jinqing Huang, Tao Huang, Muyan Xiao, Tao Xiong, Mingyong Xie
Summary: This study successfully improved the ACE inhibitory activity of black sesame seeds by fermenting them with Lactobacillus Plantarum NCU116 and hydrolyzing them using acid protease. The RF-PSO model was used to predict the ACE inhibitory activity during the hydrolysis process. Eight peptides with ACE inhibitory activity were identified from fermented black sesame seed hydrolysates after separation and screening.
Article
Chemistry, Applied
Yao Liu, Songyi Lin, Kexin Liu, Shan Wang, Qiaozhen Liu, Na Sun
Summary: This study analyzed the structural changes of shrimp proteins during digestion, predicted the immunodominant epitopes, and validated their allergenicity. The results showed that shrimp proteins were degraded into peptides during digestion, but still carried IgE epitopes that trigger allergic reactions.
Article
Chemistry, Applied
Tiantian Fu, Hongwei Cao, Yu Zhang, Xiao Guan
Summary: This study investigates the impact of milling on the active components in rice, with a focus on the stability and bioaccessibility of phenols, VB1, and alpha-GABA during cooking and digestion. The findings show that milling exacerbates the instability of gamma-GABA during cooking and VB1 during digestion, and it affects the bioaccessibility of these active compounds.
Article
Chemistry, Applied
Zhihao Yang, Yanru Hou, Min Zhang, Puxin Hou, Chang Liu, Lu Dou, Xiaoyu Chen, Lihua Zhao, Lin Su, Ye Jin
Summary: This study investigated the molecular mechanism of feeding regimes on lamb flavor by using TMT labeling combined with MS. The results showed that pasture-fed groups had higher levels of amino acids and volatile flavor substances compared to concentrate-fed groups. Additionally, several differentially abundant proteins associated with lamb flavor were identified.
Article
Chemistry, Applied
Zixuan Xie, De Zhang, Junyu Zhu, Qianqian Luo, Jun Liu, Jingtao Zhou, Xiaoyong Wang, Yuqiong Chen, Zhi Yu, Dejiang Ni
Summary: This study investigated the acidification of aroma-enhanced black tea during storage. Analysis of non-volatile substances and organic acids using UPLC-Q-TOF/MS and HPLC revealed a decrease in soluble sugars and amino acids, while an increase in organic acids such as oxalic acid, malic acid, and quinic acid. In vitro experiments further demonstrated that the acidification is a result of the decomposition of sugars and amino acids by heating, as well as the oxidation of aromatic aldehydes. Additionally, the study showed that the taste composition of tea infusion is altered, with reduced amino acids, catechins, soluble sugars, and flavonoids. This research provides a theoretical basis for improving the quality of black tea.
Article
Chemistry, Applied
Lin Wang, Falai Ma, Zihan Li, Yan Zhang
Summary: This study developed time-temperature integrators based on amyloglucosidase@Cu3(PO4)2 nanoflowers for monitoring the freshness of chilled pork. The results showed that the integrators were highly reliable and accurate in predicting the quality of chilled pork.
