期刊
FOOD CHEMISTRY
卷 138, 期 2-3, 页码 1079-1086出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2012.11.091
关键词
Citrus unshiu peel, Pectic-type polysaccharide; Base-catalysed beta-elimination; Peyer's patch; Intestinal immune system modulating activity
资金
- Korea National University of Transportation, Korea
An intestinal immune system modulating polysaccharide (CUI-3IIb-3-2, 18 kDa) was purified from Citrus unshiu peel. CUI-3IIb-3-2 mainly comprised GalA, GlcA, Ara, Gal and Rha, and it consisted of 4-linked GalA, terminal Araf, 4- or 5-linked/3,4- or 3,5-branched Ara, terminal Gal, and 2-linked/2,4-branched Rha. After CUI-3IIb-3-2 digestion by endo-alpha-D-(1 -> 4)-polygalacturonase, its hydrolysate was fractionated into PG-1 and PG-2. Methylation analyses of PG-1 and PG-2 using base-catalysed beta-elimination suggested that CUI-3IIb-3-2 be assumed as pectic-type polysaccharide. Since the activities of PG-1 and PG-2 were potently decreased, the whole polysaccharide structure of CUI-3IIb-3-2 would be essential to maintain the activity. Meanwhile, when CUI-3IIb was orally administered in mice, bone marrow cell proliferation and GM-CSF/IL-6 production from Peyer's patch cell were significantly higher (1.76- and 2.03/2.51-fold, respectively) than a saline. Therefore, a pectic-type polysaccharide from citrus peel could stimulate Peyer's patches and produce hematopoietic growth factors resulted in bone marrow cell proliferation. (C) 2012 Elsevier Ltd. All rights reserved.
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