期刊
FOOD CHEMISTRY
卷 134, 期 4, 页码 2526-2531出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2012.04.083
关键词
Chloramphenicol; Monoclonal antibody; Immunomagnetic assay; Drug residue
资金
- National Natural Science Foundation [81173017, 31101277]
- National High-Tech Research and Development Program of China (863 Program) [2010AA10Z402, 2007AA06A407]
- Tianjin Science and Technology Program [09ZCKFSH07500]
- Ministry of Science and Technology of China [SQ2009GJA0002591]
- Fundamental Research Funds for the Central Universities [65011121]
In this research, magnetic beads-based enzyme immunoassays were investigated for rapid analysis of chloramphenicol (CAP) in milk. To improve sensitivity of CAP determination, two kinds of immunomagnetic separation methods were designed and compared. Magnetic polystyrene microspheres were conjugated with anti-CAP antibody (Method I) or goat-anti-mouse IgG (Method II). The whole determination could be finished in 1.25 h. Both methods showed high sensitivity to CAP in buffer, and obtained an IC50 value of 0.05 ngmL(-1) for Method I and 0.4 ngmL(-1) for Method H. The methods showed high specificity, only showing a little cross-reaction towards CAP succinate. The two methods were applied to detect CAP in milk. The recovery rates were 80-106% and the coefficients of variation (CVs) were 4.7-15%. The immunomagnetic assay showed promising potential in rapid screening field for CAP analysis. Between the two methods, Method I is more sensitive, and Method II is more suitable for producing a general assay by changing a primary antibody for another analyte. (C) 2012 Elsevier Ltd. All rights reserved.
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