Compositions and yield of lipids extracted from hepatopancreas of Pacific white shrimp (Litopenaeus vannamei) as affected by prior autolysis

Compositions and yield of lipids extracted from hepatopancreas of Pacific white shrimp (Litopenaetis vannamei) subjected to autolysis at 60 degrees C for different times (0, 30, 60,90 120 and 150 min) were investigated. Extraction yield increased from 7.4% to 8.8% as autolysis time increased from 0 to 150 mm. Coincidental increase in total carotenoid content was obtained with increasing autolysis time (p < 0.05). The increases in thiobarbituric acid-reactive substances (TBARS) and p-anisidine value (AV) of lipids were noticeable when autolysis time increased (p < 0.05). However, no changes in free fatty acid (FFA) content were observed within the first 60 min of autolysis (p > 0.05), but subsequently increased up to 150 min. (p < 0.05). No differences in fatty acid profiles of lipids extracted from hepatopancreas without and with 60 min prior autolysis were observed. Lipids extracted contained docosahexaenoic acid (DNA: C22:6(n - 3)) as the most abundant fatty acid, followed by eicosapentaenoic acid (EPA: C20:5(n - 3)). Therefore, prior autolysis at 60 degrees C for 60 min increased the extraction yield without negative effect on lipid quality. (C) 2012 Elsevier Ltd. All rights reserved.