期刊
FOOD CHEMISTRY
卷 134, 期 2, 页码 1211-1219出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2012.02.177
关键词
Thunnus alalunga; Proteomics; Sarcoplasmic; Myofibrillar; By-product; Food protein
资金
- Ministerio de Educacion y Ciencia [CSD2007-00063]
- Comunidad de Madrid [ALIBIRD S2009/AGR-1469]
- Department of Agriculture, Fisheries, and Food of the Basque Government
- Fundacion Centros Tecnologicos-Inaki Goenaga
Proteomic approaches have been used to identify the main proteins present in processing by-products generated by the canning tuna-industry, as well as in by-products derived from filleting of skeletal red muscle of fresh tuna. Following fractionation by using an ammonium sulphate precipitation method, three proteins (tropomyosin, haemoglobin and the stress-shock protein ubiquitin) were identified in the highly heterogeneous and heat-treated material discarded by the canning-industry. Additionally, this fractionation method was successful to obtain tropomyosin of high purity from the heterogeneous starting material. By-products from skeletal red muscle of fresh tuna were efficiently fractionated to sarcoplasmic and myofibrillar fractions, prior to the identification based mainly on the combined searching of the peptide mass fingerprint (MALDI-TOF) and peptide fragment fingerprinting (MALDI LIFT-TOF/TOF) spectra of fifteen bands separated by 1D SDS-PAGE. Thus, the sarcoplasmic fraction contained myoglobin and several enzymes that are essential for efficient energy production. whereas the myofibrillar fraction had important contractile proteins, such as actin, tropomyosin, myosin or an isoform of the enzyme creatine kinase. Application of proteomic technologies has revealed new knowledge on the composition of important by-products from tuna species, enabling a better evaluation of their potential applications. (C) 2012 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据