期刊
FOOD CHEMISTRY
卷 125, 期 3, 页码 1104-1109出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2010.09.101
关键词
Stypocaulon scoparium (S. scoparium); Total phenolic content (TPC); Polyphenols; Reverse phase high performance liquid; chromatography (RP-HPLC); Gallic acid (GA); 1,1-Diphenyl-2-picrylhydrazyl (DPPH); Radical scavenging activity (RSA), Dry alga powder
资金
- Caja Insular de Ahorros de Canarias
- Consejeria de Vivienda y Arquitectura, Agricultura, Ganaderia y Pesca y Agua del Cabildo Insular de Gran Canaria
Water, water/methanol (1/1), methanol and ethanol crude extracts from a brown alga Stypocaulon scoparium were examined for total phenolic contents (TPC) using Folin-Ciocalteu method. DPPH scavenging assay was performed to measure the radical scavenging activities (RSA) of the extracts. Results showed a significant association between the antioxidant potency and the TPC. The aqueous extract showed both, the highest antioxidant activity and highest phenolic contents. The identification and quantification of phenolic antioxidants were carried out with a rapid and simple method of reverse phase high performance liquid chromatography (RP-HPLC). This method was developed for the simultaneous analysis of 14 polyphenols, namely gallic acid, catechin, epicatechin, rutin, p-coumaric acid, myricetin, quercetin and protocatechuic, vanillic, caffeic, ferulic, chlorogenic, syringic and gentisic acids. The chromatographic separation of 14 polyphenols was achieved in less than 40 min by RP-HPLC (Varian, Pursuit XRs C18 column, 5 mu m, 250 mm x 4.6 mm) using linear gradient elution of methanol and water (0.1% formic acid) with a flow rate of 1 ml/min. Gallic acid was by far the predominant polyphenol. (C) 2010 Elsevier Ltd. All rights reserved.
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