期刊
FOOD CHEMISTRY
卷 124, 期 4, 页码 1760-1769出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2010.08.015
关键词
Grapes; Developmental stages; Chemometrics; Nuclear magnetic resonance spectroscopy; Relative quantification
资金
- ERA-PG in Portugal
- Higher Education Commission (HEC) of Pakistan
H-1 nuclear magnetic resonance (NMR) was applied for the metabolic profiling of grapes from three Portuguese cultivars including 'Trincadeira', 'Aragones', and 'Touriga Nacional', at four developmental stages. Two kinds of extraction methods including deuterated NMR solvent extraction and solid phase extraction (SPE) were used for the metabolomic analysis and all the metabolites detected in H-1 NMR were elucidated by two-dimensional NMR techniques as well as the in-house NMR chemical shift database. Multivariate data analyses were also performed to identify overall metabolic differences. Trincadeira was found different from the other two cultivars, having low phenolic contents as compared to other cultivars. The initial stages showed comparatively high phenolics and organic acid contents like caftaric and malic acid while the later stages showed higher glucose and fructose levels. Veraison was found to be a metabolically critical stage of berry development. On the basis of these findings distribution of metabolites among different cultivars at different developmental stages is discussed. (C) 2010 Elsevier Ltd. All rights reserved.
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