期刊
FOOD CHEMISTRY
卷 121, 期 1, 页码 257-263出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2009.11.081
关键词
UPLC; Tandem mass spectrometry; Flavonoids; Glucosinolates; Brassica oleracea; Sinapic acid
资金
- EU [FOOD-CT-2006-016264]
- Commissioner for University and Research of the Department of Innovation, Universities and Enterprise (Generalitat de Catalunya) [AGAUR IUE/2658/2007]
A method for the simultaneous determination of intact glucosinolates and main phenolic compounds (flavonoids and sinapic acid derivatives) in Brassica oleracea L. var. botrytis was proposed. A simplified sample extraction procedure and a UPLC separation were carried out to reduce the total time of analysis. B. oleracea samples were added with internal standards (glucotropaeolin and rutin), and extracted with boiling methanol. crude extracts were evaporated under nitrogen, redissolved in mobile phase and analysed by UPLC with double detection (ESI--MRM for glucosinolates and flavonoids, and DAD for main sinapic acid derivatives). The proposed method allowed a satisfactory quantification of main native sinapic acid derivatives, flavonoids and glucosinolates with a reduced time of analysis. (C) 2009 Elsevier Ltd. All rights reserved.
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