Article
Chemistry, Analytical
Yao Yu, Yansong Li, Ning Xu, Yonghong Zha, Xue Bai, Mingyuan Liu, Yu Zhou, Xiaolei Liu
Summary: A sensitive fluorescence ratio immunoassay was developed for the detection of the mycotoxin fumonisin B1 (FB1) in maize samples. The assay utilizes the growth of monodispersed 2-D MnO2 nanosheets on gold nanoparticles (AuNP@MnO2) and the competitive binding of FB1 with the coated FB1-BSA to the FB1 monoclonal antibody. The content of FB1 can be determined by the fluorescence ratio of blue/yellow.
Article
Chemistry, Applied
Susana Ferreyra, Ruben Bottini, Ariel Fontana
Summary: A liquid chromatography method combining DAD and FLD detectors was developed for simultaneous quantification of 32 phenolic compounds in winemaking products. An excitation wavelength of 290 nm was selected, with three different emission wavelengths recorded for detection and quantification of phenolic compounds. The combination of DAD and FLD detectors improved LODs and LOQs for specific compounds, enhancing sensitivity and reducing interfering signals.
Article
Chemistry, Applied
Hyoyoung Kim, Eun Joo Baek, Yong-Kyoung Kim, Hyejin Park, Suel Hye Hur, Jang-Eok Kim, Ho Jin Kim
Summary: In this study, a specific, reliable, and suitable method for a fast and simultaneous analysis of 335 pesticides in ginseng was developed using liquid chromatography quadrupole mass spectrometry (LC-MS/MS) and gas chromatography quadrupole mass spectrometry (GC-MS/MS). The method was validated for linearity, sensitivity, selectivity, accuracy, and precision, with limits of detection (LOD) and limits of quantification (LOQ) ranging from 0.1-5.8 μg/kg and 0.3-17.5 μg/kg, respectively. Analysis of 467 ginseng samples from 2016 to 2019 revealed pesticide residues in 304 samples, but most were below the standard. The hazard quotient (HQ) of ginseng for detected pesticides was less than 1, indicating low risk.
Article
Chemistry, Multidisciplinary
Alina Mihalcea, Sonia Amariei
Summary: Crops can be contaminated by fungi producing mycotoxins, with over 400 different mycotoxins described so far. This study evaluates the prevalence of mycotoxins in corn and corn-derived products in Romania. Fumonisins were found to be the most prevalent mycotoxin, while zearalenone had the lowest presence. The levels of these mycotoxins were within the legal limits.
APPLIED SCIENCES-BASEL
(2022)
Article
Food Science & Technology
Wenshuo Zhang, Hongda Zhong, Pingping Zhao, Aiguo Shen, Houbin Li, Xinghai Liu
Summary: Carbon quantum dots (CDs) show great potential in detecting food ingredients and contaminants due to their easy preparation, good biocompatibility, and adjustable modification. The design of probes for CDs can be categorized into three types, with the introduction of functional groups on the surface being a key factor. The intermolecular interactions of different target substances play a role in determining the choice of carbon source and synthesis method.
Review
Chemistry, Analytical
Donato Calabria, Martina Zangheri, Seyedeh Rojin Shariati Pour, Ilaria Trozzi, Andrea Pace, Elisa Lazzarini, Maria Maddalena Calabretta, Mara Mirasoli, Massimo Guardigli
Summary: The presence of hidden allergens in food products has led to the urgent need for rapid and reliable analytical methods. Portable biosensors based on immunoassays and aptamers have shown promise as alternative detection methods for food allergens.
Review
Food Science & Technology
Clara Sousa
Summary: Natural pigments are widely distributed molecules in nature and play a significant role in our daily lives. In addition to their colors, they also have relevant biological properties associated with health benefits. Edible plant leaves, although less known as a source of natural pigments, contain a high variety of chlorophylls, carotenoids, and anthocyanins. This review critically analyzes the workflow used to identify and quantify these pigments in edible plant leaves using tandem mass spectrometry. Consistency issues in the methods used to extract and analyze these compounds are addressed. Mass spectrometry is a widely employed technique in metabolomics and will be discussed in the context of pigment identification and quantification.
Article
Food Science & Technology
Simon D. Kelly, Aiman Abrahim, Peter Rinke, Andrew Cannavan
Summary: An improved procedure utilizing gas chromatography-isotope ratio mass spectrometry has successfully detected the addition of exogenous C4 plant-derived sugars to pineapple juice. Site-specific C-13 analysis of ethanol derived from pineapple sugars has proven to be a reliable method in differentiating between authentic pineapple juices and those adulterated with commercial beet and cane sucrose.
NPJ SCIENCE OF FOOD
(2021)
Article
Biochemistry & Molecular Biology
Meg Shieh, Shi Xu, Oren L. Lederberg, Ming Xian
Summary: This review summarizes current detection methods for sulfane sulfur species, including cyanolysis, chemical derivatization and mass spectrometry, proteomic analysis, fluorescent probes, and resonance synchronous/Raman spectroscopic methods. The design principles, advantages, applications, and limitations of each method are discussed, along with suggested directions for future research on these methods.
Article
Spectroscopy
Yunlian Zhang, Rui Liu, Md Mehedi Hassan, Huanhuan Li, Qin Ouyang, Quansheng Chen
Summary: A simple fluorescence resonance energy transfer (FRET)-based aptasensor was developed for sensitive detection of kanamycin (KAN) in food. The method showed high detection sensitivity, satisfactory recovery rate, and successful application for KAN detection in food samples.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Review
Food Science & Technology
Liping Hong, Mingfei Pan, Xiaoqian Xie, Kaixin Liu, Jingying Yang, Shan Wang, Shuo Wang
Summary: Food allergies have a significant impact on quality of life and can even be life-threatening, with no effective cure currently available. Avoiding allergenic foods remains the best prevention method. Development of rapid, accurate, sensitive, and reliable analysis methods, such as aptamer-based fluorescence sensors, is crucial for detecting food allergens effectively. This article provides a comprehensive review of major allergens in animal and plant foods, and summarizes the principles and applications of aptamer-based fluorescence biosensors, offering strategies for rapid and sensitive allergen detection in food matrices.
Article
Food Science & Technology
Lukas Haefner, Stefan Kalkhof, Wolfgang Jira
Summary: This study developed an HPLC-MS/MS method for detecting multiple poultry meats and identified potential marker peptides through shotgun proteomics. Twenty-eight marker peptides specific for nine targeted species were selected and validated using multiple reaction monitoring (MRM). Heat stability of all twenty-nine marker peptides was confirmed.
Article
Chemistry, Applied
Yunlian Zhang, Md Mehedi Hassan, Yawen Rong, Rui Liu, Huanhuan Li, Qin Ouyang, Quansheng Chen
Summary: An upconversion nanosensor for detecting tetracycline was developed using magnetic separation and electrostatic adsorption, with a linear detection range of 0.5-1000 ng mL(-1) and a detection limit of 0.17 ng mL(-1). This method showed great potential for tetracycline detection in food samples, providing simple operation, high sensitivity, excellent repeatability, and selectivity.
Article
Chemistry, Applied
Zhuye Shang, Jianhua Liu, Zhizhi Hu, Qingtao Meng, Yue Wang, Run Zhang, Zhiqiang Zhang
Summary: The article introduces a near infrared emitted fluorescence probe (QNP) for detecting HSO3- levels in biological systems and food samples, and demonstrates its application in live animals and food samples.
Review
Food Science & Technology
Aldrey Nathalia Ribeiro Correa, Cristiano Dietrich Ferreira
Summary: This article introduces the regulations and maximum tolerated limits for mycotoxins in Brazil, and discusses the findings of recent studies indicating that several grains intended for human consumption in Brazil exceed the mycotoxins limits. The co-occurrence of mycotoxins in different grains grown in Brazil is also addressed.
FOOD REVIEWS INTERNATIONAL
(2023)
Review
Biochemistry & Molecular Biology
Angelina Pena, Sofia Duarte, Andre M. P. T. Pereira, Liliana J. G. Silva, Celia S. M. Laranjeiro, Marta Oliveira, Celeste Lino, Simone Morais
Summary: This study reviews the human biomonitoring data on mycotoxins detected in the Portuguese population and analyzes the exposure patterns and potential health risks. By addressing the difficulties and challenges of HBM studies in Portugal, this research aims to support national and European health policies.
Review
Biochemistry & Molecular Biology
Angelina Pena, Sofia Duarte, Andre M. P. T. Pereira, Liliana J. G. Silva, Celia S. M. Laranjeiro, Marta Oliveira, Celeste Lino, Simone Morais
Summary: This study presents a comprehensive review of human biomonitoring data in Portugal, focusing on the concentrations of polycyclic aromatic hydrocarbons, metals, metalloids, and pesticides in different biological matrices. The analysis of these data allows for an assessment of the exposure patterns and potential health risks among the Portuguese population. Additionally, the study identifies the main difficulties and challenges in conducting human biomonitoring studies and aims to support national and European policies promoting human health.
Article
Food Science & Technology
Paula Llorens, Renata Pietrzak-Fiecko, Juan Carlos Molto, Jordi Manes, Cristina Juan
Summary: Validated extraction methods are necessary for obtaining suitable bioaccessibility studies of mycotoxins in bakery products. The bakery industry produces bread with different ingredients to enrich its nutritional properties and protect it from fungal growth. Therefore, an extraction method was developed to analyze the legislated mycotoxins in digested phases of two types of bread.
Article
Food Science & Technology
Laura Escriva, Fojan Agahi, Pilar Vila-Donat, Jordi Manes, Giuseppe Meca, Lara Manyes
Summary: The presence of mycotoxins in cereals and cereal products is a significant issue. This study evaluated the bioaccessibility of aflatoxin B-1 (AFB1) and ochratoxin (OTA) in bread and investigated the effects of milk whey and pumpkin in reducing mycotoxins bioaccessibility. The results showed that pumpkin powder was the most effective in reducing AFB1 and OTA bioaccessibility, followed by whey, fermented whey, and the combination of pumpkin-fermented whey. These findings suggest that pumpkin and milk whey could be potential bioactive ingredients for the bakery industry.
Article
Food Science & Technology
Alexandra Silva, Andre Pereira, Liliana Silva, Angelina Pena
Summary: This study evaluated the inorganic arsenic content of Portuguese rice samples and found that some samples exceeded the maximum residue limit for infant consumption. The estimated arsenic intake for children may exceed the safe threshold, but other sources of arsenic exposure should also be considered.
Article
Food Science & Technology
Sofia Duarte, Liliana J. G. Silva, Andre M. P. T. Pereira, Marta Gimbi, Cristiane Cesar, Vanessa Vidal, Rita Basilio, Anabela Almeida, Celeste Lino, Angelina Pena
Summary: This pilot survey assessed the exposure of breastfed infants to mycotoxins in Angola and found that all breast milk samples analyzed were contaminated with mycotoxins. The contamination levels were lower for aflatoxin M1 and higher for ochratoxin A compared to previous studies. The study also identified a significant association between the consumption of cookies by lactating mothers and the levels of mycotoxin in breast milk.
Article
Food Science & Technology
Paula Llorens Castello, Ana Juan-Garcia, Juan Carlos Molto Cortes, Jordi Manes Vinuesa, Cristina Juan Garcia
Summary: This study evaluated the bioaccessibility and bioavailability of three legislated mycotoxins in two breads. The presence of other components and the co-presence of mycotoxins were found to affect the final bioavailability.
Article
Food Science & Technology
Nuria Dasi-Navarro, Manuel Lozano, Sabrina Llop, Ana Esplugues, Alessandra Cimbalo, Guillermina Font, Lara Manyes, Jordi Manes, Pilar Vila-Donat
Summary: Mycotoxin contamination in food is a global health concern. This study developed a sensitive method using LC-Q-TOF-MS for the simultaneous determination of targeted mycotoxins and the detection of untargeted ones in urine samples. The method was successfully applied to confirm internal exposure in women.
Article
Pharmacology & Pharmacy
Ines Garcia, Diana Valente, Nuno Carolino, Helder Dinis, Rui Sousa, Sofia C. Duarte, Liliana J. G. Silva, Andre M. P. T. Pereira, Angelina Pena
Summary: The Azorean dairy industry is affected by the proliferation of fungi and mycotoxin contamination in the feed. This can result in reduced reproductive performance and decreased productivity and quality of milk produced. It is important to implement strategies to reduce mycotoxin concentration and monitor their levels in food.
Article
Food Science & Technology
Liliana J. G. Silva, Andre M. P. T. Pereira, Sofia Duarte, Ines Pedro, Catarina Perdigao, Alexandra Silva, Celeste M. Lino, Anabela Almeida, Angelina Pena
Summary: Rice is a crucial staple crop for billions of people, but its consumption can lead to exposure to chemical contaminants such as mycotoxins and metalloids. This study evaluated the presence and human exposure to AFB1, OTA, ZEN, and InAs in Portuguese rice samples. AFB1 was found in 4.8% of the samples, exceeding the European MPL, while ZEN was present in 88.89% of the samples. InAs was detected in all samples, but none exceeded the MPL. There was no correlation between mycotoxin and InAs contamination. AFB1 exceeded the provisional maximum tolerable daily intake, with children being the most vulnerable group.
Article
Food Science & Technology
Ana Rita Soares Mateus, Carmen Crisafulli, Matilde Vilhena, Silvia Cruz Barros, Angelina Pena, Ana Sanches Silva
Summary: Herbal infusions are popular beverages known for their health benefits and antioxidants. This study developed a method to determine tropane alkaloids in herbal infusions, while also evaluating their antioxidant capacity. The presence of atropine in one of the samples exceeded the European regulation. Yerba mate, lemon balm, and peppermint were found to have high antioxidant capacity.
Article
Food Science & Technology
Rui R. Martins, Andre M. P. T. Pereira, Liliana J. G. Silva, Alexandra Esteves, Sofia C. Duarte, Andreia Freitas, Angelina Pena
Summary: This study aimed to evaluate the presence of nine coccidiostats in 62 egg samples using UHPLC-MS/MS. The results showed that only diclazuril and nicarbazin were detected, with nicarbazin being more frequently detected. Home-raised eggs had higher levels of nicarbazin compared to diclazuril. Overall, consumers' health should not be adversely affected by egg consumption.
Article
Food Science & Technology
Tiago de Melo Nazareth, Jorge Calpe, Carlos Luz, Jordi Manes, Giuseppe Meca
Summary: This study aimed to isolate, identify, characterize, and quantify novel lactic acid bacteria (LAB) with antifungal activity from natural sources and develop a meat product ingredient. The screening process involved assessing the antifungal activity of isolated bacteria using overlay and agar diffusion assays. The most active bacteria-free supernatants (BFS) were further quantified using the microdilution method. A meat broth with high antifungal activity was selected for ingredient preparation, and antifungal compounds were identified in the fermented meat broth. Lactiplantibacillus plantarum and Pediococcus pentosaceus were the most promising LAB, with P. pentosaceus C15 producing nonanoic acid and phenyl ethyl alcohol, as well as higher levels of lactic and acetic acid.
Article
Infectious Diseases
Andre M. P. T. Pereira, Andreia Freitas, Angelina Pena, Liliana J. G. Silva
Summary: The presence of pharmaceuticals, particularly antibiotics, in aquatic ecosystems can have significant negative impacts on natural ecosystems. Bivalves are ideal sentinel species for biomonitoring environmental health hazards in coastal and estuarine ecosystems due to their ability to filter water and bioconcentrate chemicals. An analytical strategy was developed to assess the occurrence of antibiotics as emerging pollutants in aquatic environments, and the method was validated for 43 antibiotics according to European regulations.
Article
Chemistry, Applied
M. Graca Dias, Elsa Vasco, Francisco Ravasco, Lufsa Oliveira
Summary: This study estimated the vitamin D intake of "adults" and "elderly" populations in Portugal using the TDS methodology. The results showed that the majority of people had inadequate vitamin D intake, well below the Dietary Reference Values.
Article
Chemistry, Applied
Yanan Wang, Jiachen Shi, Yong-Jiang Xu, Chin-Ping Tan, Yuanfa Liu
Summary: This study investigates the variations in lipid digestion profiles among individuals of different ages using in vitro digestion models. The findings suggest that adults have a more comprehensive lipid digestion compared to infants, and infants tend to release shorter chain length and more saturated free fatty acids during digestion. Additionally, the particle sizes in the stomach of the elderly were consistently larger. This study enhances our understanding of how lipids with different degrees of unsaturation undergo digestion in diverse age groups.
Article
Chemistry, Applied
Hyunjong Yu, Huisu Kim, Pahn-Shick Chang
Summary: Chitosan-pectin emulsion-filled hydrogel (EFH) was developed to enhance the bioaccessibility of lipophilic bioactive compounds through intestinal delivery. The EFH, prepared without crosslinking agents, demonstrated improved mechanical strength and compactness with higher pectin concentration. It retained the emulsion at pH 2.0 and released it at pH 7.4, resulting in enhanced release of free fatty acids and improved bioaccessibility of curcumin.
Article
Chemistry, Applied
Tongze Zhang, Siqi Hong, Jia-Rong Zhang, Pin-He Liu, Siyi Li, Zixian Wen, Jianwei Xiao, Guirong Zhang, Olivier Habimana, Nagendra P. Shah, Zhongquan Sui, Harold Corke
Summary: Lactic acid fermentation significantly affects the morphology and physicochemical properties of proso millet starch, including the formation of surface indentations and small pores, decrease in gelatinization temperatures, and changes in hardness and adhesiveness.
Article
Chemistry, Applied
Liqin Kong, Feng Hong, Peng Luan, Yiping Chen, Yaoze Feng, Ming Zhu
Summary: This study presents a novel impedance biosensor using composite nanomaterials and T1R1 as a signal probe, which can competitively and ultra-sensitively detect umami intensity. The biosensor exhibits exceptional analytical performance and is suitable for food flavor evaluation.
Article
Chemistry, Applied
Kunying Lv, Yixin Yang, Qilong Li, Ran Chen, Liang Deng, Yiwei Zhang, Ning Jiang
Summary: Horse's milk, with its high nutritional value and low allergenic proteins, could be a substitute for cow's milk in infant consumption. A proteomic method was used to identify and compare milk fat globule membrane (MFGM) and whey proteins from different horse breeds. The study found differences in protein composition and functionality, which could support the development of formulas more suitable for human infants.
Article
Chemistry, Applied
Enrique Jacobo Diaz-Montana, Helene Brignot, Ramon Aparicio-Ruiz, Thierry Thomas- Danguin, Maria Teresa Morales
Summary: Sensory perception of virgin olive oil is influenced by phenols and volatiles, which are affected by the composition of the oil and biological factors. This study investigated the effect of saliva and phenols on the release of volatiles, and found that the presence of phenols decreased the release of saturated volatiles.
Article
Chemistry, Applied
Wei Zhou, Rui Zhang, Zhen Cai, Fangfang Wu, Yong Hu, Chao Huang, Kun Hu, Yun Chen
Summary: Environmentally friendly and outstanding pH-responsive cationic starch nanoparticles (CSNP) were prepared from pH-sensitive starch. CSNP exhibited nanosize and regular sphere, highly free-flowing molecular chains, and demonstrated excellent pH responsiveness through multiple emulsion/demulsification transitions.
Article
Chemistry, Applied
Andrea Koo, Vinayak Ghate, Weibiao Zhou
Summary: This study suggests that direct seeding may negatively affect the nutritional quality of crops, causing a decrease in ascorbic acid, vitamin K, and total glucosinolate content.
Article
Chemistry, Applied
Tonghao Du, Yazhou Xu, Xiaoyan Xu, Shijin Xiong, Linli Zhang, Biao Dong, Jinqing Huang, Tao Huang, Muyan Xiao, Tao Xiong, Mingyong Xie
Summary: This study successfully improved the ACE inhibitory activity of black sesame seeds by fermenting them with Lactobacillus Plantarum NCU116 and hydrolyzing them using acid protease. The RF-PSO model was used to predict the ACE inhibitory activity during the hydrolysis process. Eight peptides with ACE inhibitory activity were identified from fermented black sesame seed hydrolysates after separation and screening.
Article
Chemistry, Applied
Yao Liu, Songyi Lin, Kexin Liu, Shan Wang, Qiaozhen Liu, Na Sun
Summary: This study analyzed the structural changes of shrimp proteins during digestion, predicted the immunodominant epitopes, and validated their allergenicity. The results showed that shrimp proteins were degraded into peptides during digestion, but still carried IgE epitopes that trigger allergic reactions.
Article
Chemistry, Applied
Tiantian Fu, Hongwei Cao, Yu Zhang, Xiao Guan
Summary: This study investigates the impact of milling on the active components in rice, with a focus on the stability and bioaccessibility of phenols, VB1, and alpha-GABA during cooking and digestion. The findings show that milling exacerbates the instability of gamma-GABA during cooking and VB1 during digestion, and it affects the bioaccessibility of these active compounds.
Article
Chemistry, Applied
Zhihao Yang, Yanru Hou, Min Zhang, Puxin Hou, Chang Liu, Lu Dou, Xiaoyu Chen, Lihua Zhao, Lin Su, Ye Jin
Summary: This study investigated the molecular mechanism of feeding regimes on lamb flavor by using TMT labeling combined with MS. The results showed that pasture-fed groups had higher levels of amino acids and volatile flavor substances compared to concentrate-fed groups. Additionally, several differentially abundant proteins associated with lamb flavor were identified.
Article
Chemistry, Applied
Zixuan Xie, De Zhang, Junyu Zhu, Qianqian Luo, Jun Liu, Jingtao Zhou, Xiaoyong Wang, Yuqiong Chen, Zhi Yu, Dejiang Ni
Summary: This study investigated the acidification of aroma-enhanced black tea during storage. Analysis of non-volatile substances and organic acids using UPLC-Q-TOF/MS and HPLC revealed a decrease in soluble sugars and amino acids, while an increase in organic acids such as oxalic acid, malic acid, and quinic acid. In vitro experiments further demonstrated that the acidification is a result of the decomposition of sugars and amino acids by heating, as well as the oxidation of aromatic aldehydes. Additionally, the study showed that the taste composition of tea infusion is altered, with reduced amino acids, catechins, soluble sugars, and flavonoids. This research provides a theoretical basis for improving the quality of black tea.
Article
Chemistry, Applied
Lin Wang, Falai Ma, Zihan Li, Yan Zhang
Summary: This study developed time-temperature integrators based on amyloglucosidase@Cu3(PO4)2 nanoflowers for monitoring the freshness of chilled pork. The results showed that the integrators were highly reliable and accurate in predicting the quality of chilled pork.
