期刊
FOOD CHEMISTRY
卷 117, 期 4, 页码 641-646出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2009.04.068
关键词
Medicinal fungus; Polysaccharides; Hydrolysis; Molecular weight; Antioxidant activity; Radical scavenging
资金
- Shenzhen Municipal Government for the Shenzhen Virtual University Park (SZVUP)
- Hong Kong Polytechnic University for the State Key Laboratory of Chinese Medicine and Molecular Pharmacology in Shenzhen
An exopolysaccharide (EPS) fraction isolated from mycelial culture of a Cordyceps sinensis fungus, designated EPS-1 with an average molecular weight (MW) of 38 kDa, was hydrolysed in dilute sulphuric acid solution at pH I and 90 degrees C, yielding two major MW fractions, 3.0 kDa and 30 kDa, respectively. While the proportion of lower MW fraction increased with the hydrolysis period (18% in 0.5 h and 92% in 10 h), the polydispersity (M-w/M-n) of EPS decreased steadily (from 1.47 initially to 1.10 in 10 h). The IR spectra of hydrolysed EPS fractions showed changes only in the C-O-C and C-O-H band peaks from that of EPS-1. These results suggest that the hydrolysis of EPS in the acidic solution caused the EPS degradation mainly by cleaving the glycosidic linkage but no change in the primary molecular structure. The hydrolysed EPS fractions had much higher (30-80%) antioxidant and radical-scavenging activities. (c) 2009 Elsevier Ltd. All rights reserved.
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