Article
Biochemistry & Molecular Biology
Noriko Noguchi, Yoshiro Saito, Etsuo Niki
Summary: Thiol compounds play essential roles in redox signaling and protection. Recently, the roles of persulfides and polysulfides as mediators in various physiological processes have been revealed. However, the mechanisms and dynamics are still unclear.
ANTIOXIDANTS & REDOX SIGNALING
(2023)
Article
Biochemistry & Molecular Biology
David Soriano-Castell, Antonio Currais, Pamela Maher
Summary: Ferroptosis is a form of regulated cell death dependent on iron and lipid peroxidation. Various inhibitors have been tested to distinguish ferroptosis from other forms of cell death.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Takayuki Kaneko, Yuichiro Mita, Kanako Nozawa-Kumada, Masana Yazaki, Mieko Arisawa, Etsuo Niki, Noriko Noguchi, Yoshiro Saito
Summary: This study reveals the role of hydrogen sulfide, hydropersulfides, and hydropolysulfides in cell signaling and protection against oxidative stress. The mechanism of action involves both two-electron and one-electron redox reactions, with the former receiving more attention. The study also highlights the antioxidant effects of these compounds in inhibiting lipid peroxidation.
FREE RADICAL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Azza A. A. Attia, Jehan M. M. Sorour, Neama A. A. Mohamed, Tagreed T. T. Mansour, Rasha A. A. Al-Eisa, Nahla S. S. El-Shenawy
Summary: This study aimed to investigate the protective role of vitamin E in improving cardiotoxicity induced by cyclophosphamide in rats. The results showed that vitamin E could attenuate the elevation of cardiac markers, increase in lipid profiles, reduction in antioxidant enzyme activities, and increase in lipid peroxidation. In addition, vitamin E was found to improve the histopathological alterations caused by cyclophosphamide.
Review
Biochemistry & Molecular Biology
Geraldine Veeckmans, Emily Van San, Tom Vanden Berghe
Summary: Ferroptosis is a cell death mechanism that occurs through excessive peroxidation of polyunsaturated fatty acids within cellular membranes, catalyzed by iron. This process is tightly regulated by lipid, iron, and redox metabolic processes. This review provides an overview of how ferroptosis is executed, including the major classes of inducers and inhibitors, as well as methods for detection. The detrimental role of ferroptosis in disease is also briefly discussed.
Review
Pharmacology & Pharmacy
Parisa Sadat Delghandi, Vahid Soleimani, Bibi Sedigheh Fazly Bazzaz, Hossein Hosseinzadeh
Summary: This review focuses on the oxidant and antioxidant effects of different classes of antibacterial agents and the mechanisms of oxidative stress. Some of these agents have beneficial effects on killing bacteria due to their antioxidant or oxidant effects. However, some of their side effects may be due to their oxidative effects. It is concluded that some antibacterial agents have oxidant and other antioxidant effects, which may affect their therapeutic effects or side effects. Clarifying the exact oxidant and antioxidant effects of some antimicrobial agents needs more research projects.
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
(2023)
Article
Chemistry, Applied
Yuanyuan Hu, Guanhua Zhao, Fawen Yin, Zhongyuan Liu, Jialiang Wang, Lei Qin, Dayong Zhou, Fereidoon Shahidi, Beiwei Zhu
Summary: The study found that roasting significantly increased the oxidation products in scallop lipids, but pretreatment with natural antioxidants extracted from bamboo leaves effectively prevented the formation of aldehydes.
Article
Food Science & Technology
Phung Diep Huy Vu, Akkaratch Rodklongtan, Pakamon Chitprasert
Summary: The study found that the encapsulant weight ratio of whey protein isolate-lignin complex significantly affects the survival rate of spray-dried probiotics, with the 9:1 ratio offering the best protection. Lignin improves the survival of probiotics by enhancing the structure and antioxidant properties of WPI.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Alireza Behnamifar, Shaban Rahimi, Mohammad Amir Karimi Torshizi, Mohsen Sharafi, J. L. Grimes
Summary: This study investigated the effects of alpha-lipoic acid supplementation on the semen quality and fertility parameters of aging breeder roosters, finding that appropriate levels of ALA can improve sperm quality, while higher doses do not result in further improvement.
Review
Biochemistry & Molecular Biology
Xin Chen, Xuan Li, Xiaodong Xu, Luxiao Li, Ningning Liang, Lili Zhang, Jingwen Lv, Yun-Cheng Wu, Huiyong Yin
Summary: Cardiovascular disease (CVD) is a major cause of morbidity and mortality worldwide, with myocardial infarction caused by atherosclerosis being a leading cause of death in CVD. Ferroptosis, a newly identified form of regulated cell death characterized by iron-dependent accumulation of lipid hydroperoxides, appears to play a role in CVD, exhibiting distinct features from apoptosis, necrosis, and autophagy. The review summarized recent progress on ferroptosis in CVD and atherosclerosis, emphasizing the role of free radical lipid peroxidation (LPO) and discussing the evidence and challenges in this field.
FREE RADICAL RESEARCH
(2021)
Review
Biochemistry & Molecular Biology
Onchuma Mueangson, Aongart Mahittikorn, Nsoh Godwin Anabire, Wanida Mala, Manas Kotepui
Summary: This study examined the relationship between malondialdehyde (MDA) concentrations and Plasmodium infections. The results showed that MDA concentrations were significantly elevated in patients with malaria compared to uninfected controls, indicating an increase in oxidative stress and lipid peroxidation. MDA levels can be a valuable biomarker for evaluating these processes in individuals with malaria.
Review
Biochemistry & Molecular Biology
Albert W. Girotti
Summary: This review highlights the ability of nitric oxide (NO) to antagonize antitumor photodynamic therapy (PDT) and discusses the mechanisms by which NO protects cancer cells against photokilling. It also addresses the upregulation of inducible NO synthase (iNOS) after a photodynamic challenge and its role in enhancing resistance and aggressiveness in surviving cancer cells. Strategies for overcoming these negative effects through NO-mediated resistance mechanisms are discussed.
PHOTOCHEMISTRY AND PHOTOBIOLOGY
(2021)
Article
Medicine, Research & Experimental
Luis Alberto Ayala-Ruiz, Luis Gerardo Ortega-Perez, Jonathan Saul Pinon-Simental, Oliver Rafid Magana-Rodriguez, Esperanza Melendez-Herrera, Patricia Rios-Chavez
Summary: This study reveals that 1,8-Cineole, limonene and alpha-terpineol found in Callistemon citrinus attenuated oxidative stress through antioxidant and anti-inflammatory mechanisms in rats fed with a high-fat-sucrose diet. These terpenes showed different effects on hepatic cells and oxidative biomarkers, but exhibited the same positive effects on pro-inflammatory cytokines. The findings highlight the significant anti-inflammatory effects of these terpenes and their importance in the activities of Callistemon citrinus.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Biotechnology & Applied Microbiology
Huan Xiao, Shitao Peng, Xianbin Liu, Jianna Jia, Han Wang
Summary: The study investigated the integrated influence of pollutant concentrations on phytoremediation efficiency by aquatic macrophytes. Results showed that COD removal efficiency increased with pollutant concentration, while TN, NH4+-N and TP purification efficiencies were inhibited at high concentration levels. Physiological responses in submerged macrophytes were explored to estimate the correlation between them and pollutant concentrations, with oxidative stress proved by higher MDA content. Antioxidant enzyme responses were induced to maintain plant health, but were inhibited in high pollutant concentration levels, leading to unhealthy symptoms in plants. The study concludes that excessive concentrations of organics and nutrients may inhibit adaptive physiological regulations and reduce phytoremediation efficiency.
ENVIRONMENTAL TECHNOLOGY & INNOVATION
(2021)
Article
Biochemistry & Molecular Biology
Sung Ho Lim, Chang-Ik Choi
Summary: Oxidative stress is linked to various diseases, and studies have shown that raspberry ketone exhibits antioxidant properties by increasing total antioxidant capacity, upregulating antioxidant enzymes, and improving lipid peroxidation. Further clinical studies are needed to verify its value as an effective antioxidant and therapeutic agent.
Article
Pharmacology & Pharmacy
Takuya Imatoh, Kimie Sai, Yoshiro Saito
JOURNAL OF CLINICAL PHARMACOLOGY
(2020)
Meeting Abstract
Biochemistry & Molecular Biology
Yoshiro Saito
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Yoshiro Saito
Summary: Lipid peroxidation and its products play a significant role in physiological and pathophysiological conditions, with mechanisms including enzymatic oxidation, free radical-mediated oxidation, and nonradical-mediated oxidation. The products of lipid peroxidation can lead to cytotoxicity and novel biological activity, while the antioxidant system, particularly selenoproteins and vitamin E, plays a crucial role in preventing lipid peroxidation and ferroptosis.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2021)
Article
Multidisciplinary Sciences
Takashi Toyama, Takayuki Hoshi, Takuya Noguchi, Yoshiro Saito, Atsushi Matsuzawa, Akira Naganuma, Gi-Wook Hwang
Summary: Methylmercury-induced neuronal cell death in the mouse brain may be associated with activation of the ASK1/p38 pathway by mitochondrial ROS, leading to TNF-alpha expression and microglia involvement.
SCIENTIFIC REPORTS
(2021)
Article
Pharmacology & Pharmacy
Md. Golam Kibria, Yoko Akazawa-Ogawa, Yoshihisa Hagihara, Yutaka Kuroda
Summary: The study demonstrates that immunization with amorphous aggregates of a misfolded VHH can induce a strong serum IgG response against VHH-7D12 antibodies, with a specific focus on the CDRs. The long-term immune response established a strong memory response against native VHH-7D12, but a weaker response against native VHH analogs.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2021)
Article
Biochemistry & Molecular Biology
Md Golam Kibria, Akari Fukutani, Yoko Akazawa-Ogawa, Yoshihisa Hagihara, Yutaka Kuroda
Summary: The study found that under thermal stress conditions, lysine SEP tags have a more potent solubilization effect compared to arginine SEP tags, with no impact on the structural and functional properties of the protein.
Review
Nutrition & Dietetics
Yoshiro Saito
Summary: SELENOP is a selenium-containing protein primarily produced in the liver, playing a pivotal role in selenium metabolism and antioxidative defense. It can reduce phospholipid hydroperoxides and deliver selenium to other tissues, but decreased or excessive levels can cause various dysfunctions. This review focuses on SELENOP's selenium transport system, molecular mechanism, and its biological significance.
FRONTIERS IN NUTRITION
(2021)
Article
Chemistry, Analytical
Yeji Kim, Yuuki Inoue, Hijiri Hasegawa, Yasukazu Yoshida, Toshiya Sakata
Summary: This study introduces a novel method for detecting DNA methylation through conformational transition to G-quadruplex, using a solution-gated field-effect transistor (SG-FET) without the need for labeled materials. The method involves grafting a dense hydrophilic polymer brush onto the SG-FET sensor surface to reduce electrical noise and achieve high signal-to-noise ratio analysis of DNA sequences.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Yoshiro Saito
Summary: Vitamin E is a potent lipid-soluble antioxidant, consisting of tocopherol and tocotrienol. They have different isoforms which exhibit similar radical scavenging abilities, but offer unique cytoprotective effects in cells. The review focuses on the actions of vitamin E against oxidative stress and its relation to cellular uptake and distribution, as well as its role in preventing lipid peroxidation and ferroptosis.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Cell Biology
Machiko Honda, Tatsuya Segawa, Kiyoshi Ishikawa, Masahiro Maeda, Yoshiro Saito, Shigeyuki Kon
Summary: Blood levels of nephronectin (Npnt), a protein critical for kidney development, are elevated in EAE mice. Treatment with anti-Npnt antibody inhibits EAE development. The interaction between Npnt and SeP regulates the Th17/Treg balance via the ROS level.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Nanako Kitabayashi, Shohei Nakao, Yuichiro Mita, Kotoko Arisawa, Takayuki Hoshi, Kiyo-aki Ishii, Toshinari Takamura, Noriko Noguchi, Yoshiro Saito, Takashi Toyama
Summary: Selenoprotein P (SELENOP) is a selenium-containing protein that plays a significant role in pancreatic beta cell function by maintaining the levels of cellular selenoproteins. Knockdown of the Selenop gene resulted in decreased cell viability, insulin levels, and insulin secretion, which were recovered by the addition of selenium. These findings suggest that SELENOP expression is important for the function of pancreatic beta cells.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Allergy
Hirohisa Ogawa, Masahiko Azuma, Aya Umeno, Mayuko Shimizu, Kazutoshi Murotomi, Yasukazu Yoshida, Yasuhiko Nishioka, Koichi Tsuneyama
Summary: In this study, the roles of neutrophils and nerve growth factor (NGF) in refractory asthma were examined. The results showed that neutrophil myeloperoxidase (MPO)-derived singlet oxygen induces increased NGF production, leading to airway hyperreactivity (AHR) and the production of specific markers. These findings contribute to the development of new therapies and biomarkers for non-type 2 and refractory asthma.
ALLERGOLOGY INTERNATIONAL
(2022)
Review
Environmental Sciences
Marco Vinceti, Tommaso Filippini, Ewa Jablonska, Yoshiro Saito, Lauren A. Wise
Summary: Recent evidence suggests that previous research underestimated the adverse effects of selenium exposure at low levels and the potential drawbacks of maximizing selenoprotein expression. This calls for a reassessment of the recommended range of selenium exposure and dietary reference values.
ENVIRONMENTAL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Teresa Urbano, Marco Vinceti, Jessica Mandrioli, Annalisa Chiari, Tommaso Filippini, Roberta Bedin, Manuela Tondelli, Cecilia Simonini, Giovanna Zamboni, Misaki Shimizu, Yoshiro Saito
Summary: This study found abnormalities in the concentrations of selenium-transporter protein selenoprotein P in the central nervous system and blood, which are associated with amyotrophic lateral sclerosis (ALS) and neurocognitive disorders.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Takayuki Kaneko, Yuichiro Mita, Kanako Nozawa-Kumada, Masana Yazaki, Mieko Arisawa, Etsuo Niki, Noriko Noguchi, Yoshiro Saito
Summary: This study reveals the role of hydrogen sulfide, hydropersulfides, and hydropolysulfides in cell signaling and protection against oxidative stress. The mechanism of action involves both two-electron and one-electron redox reactions, with the former receiving more attention. The study also highlights the antioxidant effects of these compounds in inhibiting lipid peroxidation.
FREE RADICAL RESEARCH
(2022)
Article
Chemistry, Applied
M. Graca Dias, Elsa Vasco, Francisco Ravasco, Lufsa Oliveira
Summary: This study estimated the vitamin D intake of "adults" and "elderly" populations in Portugal using the TDS methodology. The results showed that the majority of people had inadequate vitamin D intake, well below the Dietary Reference Values.
Article
Chemistry, Applied
Yanan Wang, Jiachen Shi, Yong-Jiang Xu, Chin-Ping Tan, Yuanfa Liu
Summary: This study investigates the variations in lipid digestion profiles among individuals of different ages using in vitro digestion models. The findings suggest that adults have a more comprehensive lipid digestion compared to infants, and infants tend to release shorter chain length and more saturated free fatty acids during digestion. Additionally, the particle sizes in the stomach of the elderly were consistently larger. This study enhances our understanding of how lipids with different degrees of unsaturation undergo digestion in diverse age groups.
Article
Chemistry, Applied
Hyunjong Yu, Huisu Kim, Pahn-Shick Chang
Summary: Chitosan-pectin emulsion-filled hydrogel (EFH) was developed to enhance the bioaccessibility of lipophilic bioactive compounds through intestinal delivery. The EFH, prepared without crosslinking agents, demonstrated improved mechanical strength and compactness with higher pectin concentration. It retained the emulsion at pH 2.0 and released it at pH 7.4, resulting in enhanced release of free fatty acids and improved bioaccessibility of curcumin.
Article
Chemistry, Applied
Tongze Zhang, Siqi Hong, Jia-Rong Zhang, Pin-He Liu, Siyi Li, Zixian Wen, Jianwei Xiao, Guirong Zhang, Olivier Habimana, Nagendra P. Shah, Zhongquan Sui, Harold Corke
Summary: Lactic acid fermentation significantly affects the morphology and physicochemical properties of proso millet starch, including the formation of surface indentations and small pores, decrease in gelatinization temperatures, and changes in hardness and adhesiveness.
Article
Chemistry, Applied
Liqin Kong, Feng Hong, Peng Luan, Yiping Chen, Yaoze Feng, Ming Zhu
Summary: This study presents a novel impedance biosensor using composite nanomaterials and T1R1 as a signal probe, which can competitively and ultra-sensitively detect umami intensity. The biosensor exhibits exceptional analytical performance and is suitable for food flavor evaluation.
Article
Chemistry, Applied
Kunying Lv, Yixin Yang, Qilong Li, Ran Chen, Liang Deng, Yiwei Zhang, Ning Jiang
Summary: Horse's milk, with its high nutritional value and low allergenic proteins, could be a substitute for cow's milk in infant consumption. A proteomic method was used to identify and compare milk fat globule membrane (MFGM) and whey proteins from different horse breeds. The study found differences in protein composition and functionality, which could support the development of formulas more suitable for human infants.
Article
Chemistry, Applied
Enrique Jacobo Diaz-Montana, Helene Brignot, Ramon Aparicio-Ruiz, Thierry Thomas- Danguin, Maria Teresa Morales
Summary: Sensory perception of virgin olive oil is influenced by phenols and volatiles, which are affected by the composition of the oil and biological factors. This study investigated the effect of saliva and phenols on the release of volatiles, and found that the presence of phenols decreased the release of saturated volatiles.
Article
Chemistry, Applied
Wei Zhou, Rui Zhang, Zhen Cai, Fangfang Wu, Yong Hu, Chao Huang, Kun Hu, Yun Chen
Summary: Environmentally friendly and outstanding pH-responsive cationic starch nanoparticles (CSNP) were prepared from pH-sensitive starch. CSNP exhibited nanosize and regular sphere, highly free-flowing molecular chains, and demonstrated excellent pH responsiveness through multiple emulsion/demulsification transitions.
Article
Chemistry, Applied
Andrea Koo, Vinayak Ghate, Weibiao Zhou
Summary: This study suggests that direct seeding may negatively affect the nutritional quality of crops, causing a decrease in ascorbic acid, vitamin K, and total glucosinolate content.
Article
Chemistry, Applied
Tonghao Du, Yazhou Xu, Xiaoyan Xu, Shijin Xiong, Linli Zhang, Biao Dong, Jinqing Huang, Tao Huang, Muyan Xiao, Tao Xiong, Mingyong Xie
Summary: This study successfully improved the ACE inhibitory activity of black sesame seeds by fermenting them with Lactobacillus Plantarum NCU116 and hydrolyzing them using acid protease. The RF-PSO model was used to predict the ACE inhibitory activity during the hydrolysis process. Eight peptides with ACE inhibitory activity were identified from fermented black sesame seed hydrolysates after separation and screening.
Article
Chemistry, Applied
Yao Liu, Songyi Lin, Kexin Liu, Shan Wang, Qiaozhen Liu, Na Sun
Summary: This study analyzed the structural changes of shrimp proteins during digestion, predicted the immunodominant epitopes, and validated their allergenicity. The results showed that shrimp proteins were degraded into peptides during digestion, but still carried IgE epitopes that trigger allergic reactions.
Article
Chemistry, Applied
Tiantian Fu, Hongwei Cao, Yu Zhang, Xiao Guan
Summary: This study investigates the impact of milling on the active components in rice, with a focus on the stability and bioaccessibility of phenols, VB1, and alpha-GABA during cooking and digestion. The findings show that milling exacerbates the instability of gamma-GABA during cooking and VB1 during digestion, and it affects the bioaccessibility of these active compounds.
Article
Chemistry, Applied
Zhihao Yang, Yanru Hou, Min Zhang, Puxin Hou, Chang Liu, Lu Dou, Xiaoyu Chen, Lihua Zhao, Lin Su, Ye Jin
Summary: This study investigated the molecular mechanism of feeding regimes on lamb flavor by using TMT labeling combined with MS. The results showed that pasture-fed groups had higher levels of amino acids and volatile flavor substances compared to concentrate-fed groups. Additionally, several differentially abundant proteins associated with lamb flavor were identified.
Article
Chemistry, Applied
Zixuan Xie, De Zhang, Junyu Zhu, Qianqian Luo, Jun Liu, Jingtao Zhou, Xiaoyong Wang, Yuqiong Chen, Zhi Yu, Dejiang Ni
Summary: This study investigated the acidification of aroma-enhanced black tea during storage. Analysis of non-volatile substances and organic acids using UPLC-Q-TOF/MS and HPLC revealed a decrease in soluble sugars and amino acids, while an increase in organic acids such as oxalic acid, malic acid, and quinic acid. In vitro experiments further demonstrated that the acidification is a result of the decomposition of sugars and amino acids by heating, as well as the oxidation of aromatic aldehydes. Additionally, the study showed that the taste composition of tea infusion is altered, with reduced amino acids, catechins, soluble sugars, and flavonoids. This research provides a theoretical basis for improving the quality of black tea.
Article
Chemistry, Applied
Lin Wang, Falai Ma, Zihan Li, Yan Zhang
Summary: This study developed time-temperature integrators based on amyloglucosidase@Cu3(PO4)2 nanoflowers for monitoring the freshness of chilled pork. The results showed that the integrators were highly reliable and accurate in predicting the quality of chilled pork.