Article
Food Science & Technology
Thammaporn Junsai, Saranya Poapolathep, Samak Sutjarit, Mario Giorgi, Zhaowei Zhang, Antonio Francesco Logrieco, Peiwu Li, Amnart Poapolathep
Summary: This study investigated the occurrence of multi-mycotoxins in six types of vegetable oils available in Thailand, revealing variations in mycotoxin contamination among different oil samples. The levels of analyzed mycotoxins were found to be below regulatory limits, indicating a low health risk to consumers.
Article
Chemistry, Analytical
Sophie Catanese, Raoul K. Khanna, Antoine Lefevre, Hugo Alarcan, Pierre-Jean Pisella, Patrick Emond, Helene Blasco
Summary: To apply metabo-lipidomics in ophthalmological and systemic diseases, it is necessary to standardize the preanalytical and analytical steps in human tears. This study compared different methods for each step of the workflow and found that methanol/water extraction provided the best results for metabolomics, while Folch extraction was superior for lipidomics. The use of microcapillary glass tube for sampling was superior to Schirmer strip for metabolomics. A volume of 5 mu L provided satisfactory metabo-lipidomic coverage. These findings will contribute to improving the metabo-lipidomic workflow in human tears for the identification of new biomarkers.
Article
Food Science & Technology
Xin Guan, Yuchao Feng, Decheng Suo, Zhiming Xiao, Shi Wang, Ying Liang, Xia Fan
Summary: In this study, a method for detecting 11 mycotoxins in maize was established using multiple-impurity adsorption purification (MIA) technologies and liquid chromatography-tandem mass spectrometry (LC-MS/MS). The validated method was suitable for simultaneous determination of multiple mycotoxins in maize.
Article
Environmental Sciences
Benjamin H. Maskrey, Karl Dean, Nadine Morrell, Andrew D. Turner
Summary: A simple and rapid UHPLC-MS/MS method has been developed for quantifying pharmaceuticals and metabolites in bivalve molluscs, demonstrating good performance in terms of extraction efficiencies and sensitivity.
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
(2021)
Article
Biochemical Research Methods
Shuailong Chen, Xuan Li, Shijia Liu, Longshan Zhao, Wenyong Zhang, Zhili Xiong, Hemi Luan
Summary: A rapid, sensitive, and high-throughput isotope dilution liquid chromatography-negative ion electrospray tandem mass spectrometry (IDLC-nESI-MS/MS) method was developed for accurate quantification of multiple ceramides. This method demonstrated good linearity and recovery, and could be applied for studying ceramide biology and disease diagnosis.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Jennifer Bates, Adilah Bahadoor, Sheryl A. Tittlemier, Jeremy E. Melanson
Summary: Analysis of low-level organic contaminants in complex matrices is important for global food safety monitoring. Balancing sample throughput with complex experimental designs and sample clean-up to reduce matrix effects is challenging. Multiple strategies exist to mitigate these effects, with internal standard-based methods such as isotope dilution mass spectrometry (IDMS) being the most advantageous.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Applied
Hyoyoung Kim, Eun Joo Baek, Yong-Kyoung Kim, Hyejin Park, Suel Hye Hur, Jang-Eok Kim, Ho Jin Kim
Summary: In this study, a specific, reliable, and suitable method for a fast and simultaneous analysis of 335 pesticides in ginseng was developed using liquid chromatography quadrupole mass spectrometry (LC-MS/MS) and gas chromatography quadrupole mass spectrometry (GC-MS/MS). The method was validated for linearity, sensitivity, selectivity, accuracy, and precision, with limits of detection (LOD) and limits of quantification (LOQ) ranging from 0.1-5.8 μg/kg and 0.3-17.5 μg/kg, respectively. Analysis of 467 ginseng samples from 2016 to 2019 revealed pesticide residues in 304 samples, but most were below the standard. The hazard quotient (HQ) of ginseng for detected pesticides was less than 1, indicating low risk.
Article
Food Science & Technology
Hongping Chen, Xinlu Wang, Pingxiang Liu, Qi Jia, Haolei Han, Changling Jiang, Jing Qiu
Summary: A modified QuEChERS method using ultra performance liquid chromatography tandem mass spectrometry was developed for the determination of three typical metabolites of pyrethroid pesticides in tea. The method was validated with good accuracy and precision, and 3-PBA and TFA were detected in two out of 22 tea samples.
Article
Chemistry, Analytical
Xinyue Zheng, Xujian Duan, Di Lu, Qiuhong Jiang, Yajun Liu, Hongyu Xue, Jiansong You, Lei Yin, Meiyun Shi
Summary: This study developed a highly selective and sensitive ultra-performance liquid chromatography-tandem mass spectrometry method to determine butachlor in a biological matrix. The method used tandem mass spectrometry cubed scanning mode, improving selectivity and sensitivity. The results indicated that butachlor was cellular toxic.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Biochemical Research Methods
Bob Challen, Michael Morris, Rainer Cramer
Summary: High-throughput mass spectrometry is a rapidly growing field, and liquid atmospheric pressure-matrix-assisted laser desorption/ionization (LAPMALDI) MS is proposed as an alternative for ultra-high-throughput protein analysis. By using a high-speed XY-stage actuator, sample acquisition rates of up to 10 samples per second and a data acquisition rate of 200 spectra per scan can be achieved.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
Article
Food Science & Technology
Daniela Delfino, Dario Lucchetti, Tabita Mauti, Marta Mancuso, Paolo Di Giustino, Daniela Triolone, Stefano Vaccari, Rossana Claudia Bonanni, Bruno Neri, Katia Russo
Summary: This study aimed to monitor the presence of Ochratoxin A (OTA) in cheeses and pork meat products. A simple and accurate dilute and shoot method was validated for OTA detection in 75 samples, and the importance of further research on exposure and risk assessment for OTA was highlighted.
JOURNAL OF FOOD SCIENCE
(2022)
Article
Biochemical Research Methods
Hsiao-Fan Chen, Chih-Chin Kao, Shuk-Man Ka, San-Yuan Wang, Michael X. Chen, Guan-Yuan Chen, Te- Weng, Ruo-Yun Lai, Shu-Ching Yeh, Yen-Chung Lin, Hsi-Hsien Chen, Wei-Chiao Chang, Mai-Szu Wu, I-Lin Tsai
Summary: An analytical method was developed to identify plasma biomarkers for IgA nephropathy. The method involved the analysis of IgA1 hinge region glycoforms and revealed a decrease in sialylation in the IgA1 hinge region of IgAN patients, which was correlated with the estimated glomerular filtration rate.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Chemistry, Analytical
A. Arrizabalaga-Larranaga, S. Epigmenio-Chamu, F. J. Santos, E. Moyano
Summary: This paper describes the development of a rapid and sensitive method for detecting banned dyes in spice samples using UHPLC-MS/MS technology combined with three atmospheric pressure ionization sources. The proposed method demonstrated good performance in sample analysis, with low detection limits, good precision, and accurate quantitation.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biochemical Research Methods
Elizabeth Holton, Barbara Kasprzyk-Hordern
Summary: The manuscript describes a new multiresidue method using UPLC-MS/MS and MRM for the identification and quantification of antibiotics and their metabolites in various environmental matrices. The method shows good instrumental performance with high accuracy and precision. Method quantification limits are low in river water, wastewater, river sediment, and suspended solids, and it has been successfully validated for the quantification of analytes in both aqueous and solid samples. Some analytes are considered on a semi-quantitative basis.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Food Science & Technology
Aristeidis S. Tsagkaris, Nela Prusova, Zbynek Dzuman, Jana Pulkrabova, Jana Hajslova
Summary: Cereals are a widely consumed food commodity that may be contaminated by mycotoxins, leading to potential health risks for consumers and significant financial losses. A UHPLC-q-Orbitrap MS method was optimized and validated for analyzing mycotoxins in wheat, maize, and rye flour matrices, achieving low LOQ, wide linear ranges, and good recovery rates. The study highlights the applicability of HRMS detection in mycotoxin food analysis.
Review
Food Science & Technology
Araceli Rivera-Perez, Roberto Romero-Gonzalez, Antonia Garrido Frenich
Summary: This review examines the extraction and clean-up procedures for toxic compounds in spices, herbs, and tea from 2010 to 2020. Analytical methods, including chromatographic separation and mass spectrometry detection, are discussed. The occurrence of these compounds in different matrices is also summarized.
FOOD REVIEWS INTERNATIONAL
(2023)
Article
Biochemistry & Molecular Biology
Julia Baranzelli, Sabrina Somacal, Camila Sant'Anna Monteiro, Renius de Oliveira Mello, Eliseu Rodrigues, Osmar Damian Prestes, Rosalia Lopez-Ruiz, Antonia Garrido Frenich, Roberto Romero-Gonzalez, Martha Zavariz de Miranda, Tatiana Emanuelli
Summary: Pre-harvest sprouting in wheat can be simulated in the laboratory, and different wheat cultivars have distinct behavior in terms of changes in phytochemical compounds during germination. Hard wheat cultivars have higher contents of benzoxazinoids and flavonoids compared to soft wheat cultivars. The matrix-bound phytochemicals decrease only in hard-texture wheat cultivars during germination, due to decreased levels of phenolic acids and flavonoids that are bound to the cell wall. Germination also increases the content of benzoxazinoids and antioxidant capacity in wheat, enhancing its health benefits.
Review
Chemistry, Analytical
Jesus Marin-Saez, Rosalia Lopez-Ruiz, Madalena Sobral, Roberto Romero-Gonzalez, Antonia Garrido Frenich, Isabel M. P. L. V. O. Ferreira
Summary: This study conducted a systematic review of literature from January 1, 2000 to March 15, 2022, focusing on studies related to the analysis of organic chemical hazards in saliva. The review aimed to provide insights into saliva sample collection, extraction, and analytical techniques, as well as the presence of identified compounds. The findings identified significant gaps in the standardized procedures for saliva sampling/collection/storage, limiting the wider use of saliva as a valuable specimen for human biomonitoring of exposure to food chain contaminants.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Food Science & Technology
Maria Elena Hergueta-Castillo, Rosalia Lopez-Ruiz, Antonia Garrido Frenich, Roberto Romero-Gonzalez
Summary: This study evaluated the metabolism and dissipation of flutriafol in different conditions and found that it had a short half-life of less than 17 days. Seven metabolites were detected at low concentrations, and some of them were found to be more toxic than the parent compound.
Article
Chemistry, Applied
Araceli Rivera-Perez, Pascual Garcia-Perez, Roberto Romero-Gonzalez, Antonia Garrido Frenich, Luigi Lucini
Summary: A comprehensive UHPLC-QTOF-HRMS fingerprinting approach was used to trace thyme from three different production regions and evaluate the metabolic differences in response to processing. 37 key compounds related to origin and 33 key compounds related to processing differentiation were identified. The study highlights the terroir influence on thyme composition, with flavonoids, amino acids, and peptides being the most discriminant chemical classes. Thyme sterilization was found to enrich the overall metabolite content, potentially due to increased compound accessibility.
Article
Agriculture, Multidisciplinary
Jesus Marin-Saez, Rosalia Lopez-Ruiz, Roberto Romero-Gonzalez, Antonia Garrido Frenich
Summary: In this study, a method involving solid-liquid extraction and liquid chromatography-high-resolution mass spectrometry was developed to investigate the dissipation of neem oil when applied alone or together with Bacillus thuringiensis (Bt). The dissipation of azadirachtin A (AzA) was found to be faster when neem oil and Bt were applied together at 22 degrees C compared to when neem oil was applied alone at 3 degrees C. Several related compounds and unknown metabolites were also identified in the degraded samples.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Chemistry, Analytical
Rosalia Lopez-Ruiz, Antonio Jesus Maldonado-Reina, Jesus Marin-Saez, Roberto Romero-Gonzalez, Jose Luis Martinez-Vidal, Antonia Garrido Frenich
Summary: This study proposes a methodology for characterizing plant protection products (PPPs) through suspect and unknown analyses. It involves three main stages: sample preparation, separation and detection, and data analysis. The use of different solvents and chromatographic techniques, such as LC and GC coupled with HRMS analyzers, allows for accurate detection and a high number of features. Tips and recommendations for data analysis are provided to perform suspect screening and unknown analysis.
TRENDS IN ENVIRONMENTAL ANALYTICAL CHEMISTRY
(2023)
Article
Food Science & Technology
Mireya Granados-Povedano, Irene Dominguez, Francisco Egea-Gonzalez, Antonia Garrido Frenich, Francisco Javier Arrebola
Summary: A new polyvalent wide-scope analytical method has been developed and validated to detect low concentrations of 260 pesticides, non-target substances, and metabolites in both raw and processed fruits. The target approach was validated according to SANTE Guide requirements, showing satisfactory results in terms of accuracy, precision, linearity, and robustness. The method, based on QuEChERS extraction followed by GC-HRMS, achieved part-per-trillions lower limits, allowing the detection of 18 pesticides in commercial samples. An optimized non-target approach was also applied, leading to the identification of additional compounds, including pesticide metabolites not considered in the target screening.
Article
Engineering, Marine
Marina Astudillo-Pascual, Roxana Tudor, Irene Dominguez, Pedro A. Aguilera, Antonia Garrido Frenich
Summary: This study aims to fill the information gaps regarding the content of organic micropollutants in seagrass and proposes an eco-friendly extraction method. The study conducted trials with various extraction methods and used gas chromatography coupled to high resolution mass spectrometry for comprehensive analysis. The results identified several compounds with high detection frequency in environmental samples, including PAHs, PAH derivatives, UV stabilizers, plastic additives, and pesticides.
JOURNAL OF MARINE SCIENCE AND ENGINEERING
(2023)
Article
Biochemical Research Methods
Ana M. Jimenez-Carvelo, Rosalia Lopez-Ruiz, Roberto Romero-Gonzalez, Antonia Garrido-Frenich
Summary: The study shows that employing an experimental design tool facilitates rapid optimization of critical parameters in mass spectrometry. It also highlights the disadvantage of using commercial databases in compound identification and the superiority of home-made databases.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Agriculture, Multidisciplinary
Jesus Marin-Saez, Rosalia Lopez-Ruiz, Roberto Romero-Gonzalez, Antonia Garrido Frenich
Summary: This study conducted targeted and nontargeted analysis to investigate the fate of additives in tomato samples treated with two pesticides under greenhouse conditions. Most of the compounds detected degraded within 7 days after the second application.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Review
Chemistry, Analytical
Rosalia Lopez-Ruiz, Jesus Marin-Saez, Osmar D. Prestes, Roberto Romero-Gonzalez, Antonia Garrido Frenich
Summary: This paper discusses the methods for controlling anthropogenic contaminants in edible oils, including sample preparation, analysis, and regulations. Liquid-liquid extraction and QuEChERS methods are commonly used for extraction and analysis, with mass spectrometry being the most commonly used analyzer. The results show that a portion of the analyzed samples exceed the legal limits for pesticides, MCPDs, and PAHs.
CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Applied
M. Graca Dias, Elsa Vasco, Francisco Ravasco, Lufsa Oliveira
Summary: This study estimated the vitamin D intake of "adults" and "elderly" populations in Portugal using the TDS methodology. The results showed that the majority of people had inadequate vitamin D intake, well below the Dietary Reference Values.
Article
Chemistry, Applied
Yanan Wang, Jiachen Shi, Yong-Jiang Xu, Chin-Ping Tan, Yuanfa Liu
Summary: This study investigates the variations in lipid digestion profiles among individuals of different ages using in vitro digestion models. The findings suggest that adults have a more comprehensive lipid digestion compared to infants, and infants tend to release shorter chain length and more saturated free fatty acids during digestion. Additionally, the particle sizes in the stomach of the elderly were consistently larger. This study enhances our understanding of how lipids with different degrees of unsaturation undergo digestion in diverse age groups.
Article
Chemistry, Applied
Hyunjong Yu, Huisu Kim, Pahn-Shick Chang
Summary: Chitosan-pectin emulsion-filled hydrogel (EFH) was developed to enhance the bioaccessibility of lipophilic bioactive compounds through intestinal delivery. The EFH, prepared without crosslinking agents, demonstrated improved mechanical strength and compactness with higher pectin concentration. It retained the emulsion at pH 2.0 and released it at pH 7.4, resulting in enhanced release of free fatty acids and improved bioaccessibility of curcumin.
Article
Chemistry, Applied
Tongze Zhang, Siqi Hong, Jia-Rong Zhang, Pin-He Liu, Siyi Li, Zixian Wen, Jianwei Xiao, Guirong Zhang, Olivier Habimana, Nagendra P. Shah, Zhongquan Sui, Harold Corke
Summary: Lactic acid fermentation significantly affects the morphology and physicochemical properties of proso millet starch, including the formation of surface indentations and small pores, decrease in gelatinization temperatures, and changes in hardness and adhesiveness.
Article
Chemistry, Applied
Liqin Kong, Feng Hong, Peng Luan, Yiping Chen, Yaoze Feng, Ming Zhu
Summary: This study presents a novel impedance biosensor using composite nanomaterials and T1R1 as a signal probe, which can competitively and ultra-sensitively detect umami intensity. The biosensor exhibits exceptional analytical performance and is suitable for food flavor evaluation.
Article
Chemistry, Applied
Kunying Lv, Yixin Yang, Qilong Li, Ran Chen, Liang Deng, Yiwei Zhang, Ning Jiang
Summary: Horse's milk, with its high nutritional value and low allergenic proteins, could be a substitute for cow's milk in infant consumption. A proteomic method was used to identify and compare milk fat globule membrane (MFGM) and whey proteins from different horse breeds. The study found differences in protein composition and functionality, which could support the development of formulas more suitable for human infants.
Article
Chemistry, Applied
Enrique Jacobo Diaz-Montana, Helene Brignot, Ramon Aparicio-Ruiz, Thierry Thomas- Danguin, Maria Teresa Morales
Summary: Sensory perception of virgin olive oil is influenced by phenols and volatiles, which are affected by the composition of the oil and biological factors. This study investigated the effect of saliva and phenols on the release of volatiles, and found that the presence of phenols decreased the release of saturated volatiles.
Article
Chemistry, Applied
Wei Zhou, Rui Zhang, Zhen Cai, Fangfang Wu, Yong Hu, Chao Huang, Kun Hu, Yun Chen
Summary: Environmentally friendly and outstanding pH-responsive cationic starch nanoparticles (CSNP) were prepared from pH-sensitive starch. CSNP exhibited nanosize and regular sphere, highly free-flowing molecular chains, and demonstrated excellent pH responsiveness through multiple emulsion/demulsification transitions.
Article
Chemistry, Applied
Andrea Koo, Vinayak Ghate, Weibiao Zhou
Summary: This study suggests that direct seeding may negatively affect the nutritional quality of crops, causing a decrease in ascorbic acid, vitamin K, and total glucosinolate content.
Article
Chemistry, Applied
Tonghao Du, Yazhou Xu, Xiaoyan Xu, Shijin Xiong, Linli Zhang, Biao Dong, Jinqing Huang, Tao Huang, Muyan Xiao, Tao Xiong, Mingyong Xie
Summary: This study successfully improved the ACE inhibitory activity of black sesame seeds by fermenting them with Lactobacillus Plantarum NCU116 and hydrolyzing them using acid protease. The RF-PSO model was used to predict the ACE inhibitory activity during the hydrolysis process. Eight peptides with ACE inhibitory activity were identified from fermented black sesame seed hydrolysates after separation and screening.
Article
Chemistry, Applied
Yao Liu, Songyi Lin, Kexin Liu, Shan Wang, Qiaozhen Liu, Na Sun
Summary: This study analyzed the structural changes of shrimp proteins during digestion, predicted the immunodominant epitopes, and validated their allergenicity. The results showed that shrimp proteins were degraded into peptides during digestion, but still carried IgE epitopes that trigger allergic reactions.
Article
Chemistry, Applied
Tiantian Fu, Hongwei Cao, Yu Zhang, Xiao Guan
Summary: This study investigates the impact of milling on the active components in rice, with a focus on the stability and bioaccessibility of phenols, VB1, and alpha-GABA during cooking and digestion. The findings show that milling exacerbates the instability of gamma-GABA during cooking and VB1 during digestion, and it affects the bioaccessibility of these active compounds.
Article
Chemistry, Applied
Zhihao Yang, Yanru Hou, Min Zhang, Puxin Hou, Chang Liu, Lu Dou, Xiaoyu Chen, Lihua Zhao, Lin Su, Ye Jin
Summary: This study investigated the molecular mechanism of feeding regimes on lamb flavor by using TMT labeling combined with MS. The results showed that pasture-fed groups had higher levels of amino acids and volatile flavor substances compared to concentrate-fed groups. Additionally, several differentially abundant proteins associated with lamb flavor were identified.
Article
Chemistry, Applied
Zixuan Xie, De Zhang, Junyu Zhu, Qianqian Luo, Jun Liu, Jingtao Zhou, Xiaoyong Wang, Yuqiong Chen, Zhi Yu, Dejiang Ni
Summary: This study investigated the acidification of aroma-enhanced black tea during storage. Analysis of non-volatile substances and organic acids using UPLC-Q-TOF/MS and HPLC revealed a decrease in soluble sugars and amino acids, while an increase in organic acids such as oxalic acid, malic acid, and quinic acid. In vitro experiments further demonstrated that the acidification is a result of the decomposition of sugars and amino acids by heating, as well as the oxidation of aromatic aldehydes. Additionally, the study showed that the taste composition of tea infusion is altered, with reduced amino acids, catechins, soluble sugars, and flavonoids. This research provides a theoretical basis for improving the quality of black tea.
Article
Chemistry, Applied
Lin Wang, Falai Ma, Zihan Li, Yan Zhang
Summary: This study developed time-temperature integrators based on amyloglucosidase@Cu3(PO4)2 nanoflowers for monitoring the freshness of chilled pork. The results showed that the integrators were highly reliable and accurate in predicting the quality of chilled pork.