期刊
FOOD AND CHEMICAL TOXICOLOGY
卷 66, 期 -, 页码 56-64出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fct.2014.01.019
关键词
Taraxacum officinale polysaccharide; Anti-inflammatory; NF kappa B; Antioxidative; Nrf2; PI3KAkt
资金
- National Research Foundation of Korea (NRF)
- Ministry of Education, Science, and Technology [NRF-2010-0005599]
Anti-inflammatory and anti-oxidative activities of polysaccharides from Taraxacum officinale (TOP 1 and 2) were analyzed in RAW 264.7 cells. First, lipopolysaccharide (LPS) was applied to identify anti-inflammatory activity of TOPs, which reduced expression of inducible nitric oxide synthase (iNOS) and tumor necrosis factor (TNF)-alpha. TOPs treatment inhibited phosphorylation of inflammatory transcription factor, nuclear factor (NF)kappa B, and its upstream signaling molecule, PI3K/Akt. Second, cytoprotective potential of TOPs against oxidative stress was investigated via heme oxygenase (HO)-1 induction. HO-1, one of phase II enzymes shows antioxidative activity, was potently induced by TOPs treatment, which was in accordance with the nuclear translocation of nuclear factor-erythroid 2 p45-related factor 2 (Nrf2). In addition, TOPs treatment phosphorylated PI3K/Akt with slight activation of c-Jun NH2-terminal kinase (INK). TOPs-mediated HO-1 induction protected macrophage cells from oxidative stress-induced cell death, which was confirmed by SnPP and CoPP (HO-1 inhibitor and inducer, respectively). Consequently, TOPs potently inhibited NF kappa B-mediated inflammation and accelerated Nrf2-mediated antioxidative potential through the modulation of PI3K/Akt pathway, which would contribute to their promising strategy for novel anti-inflammatory and anti-oxidative agents. (C) 2014 Published by Elsevier Ltd.
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