期刊
FOOD AND CHEMICAL TOXICOLOGY
卷 50, 期 7, 页码 2407-2416出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fct.2012.04.034
关键词
Anti-cancer; Lonicera japonica Thunb.; Polyphenolic extract; PI3K/Akt; MAPKs
资金
- National Research Foundation (NRF) of Korea
- Korean government (MEST) [2009-0084454]
- National R&D Program for Cancer Control, Ministry for Health, Welfare, and Family affairs, Republic of Korea [0820050]
- National Research Foundation of Korea [2009-0062556, 전09A1117, 2012R1A2A2A06045015, 2012M3A9B8019303] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Lonicera japonica Thunb. (L japonica T.) has been used in Korean traditional medicine for long time because of its anti-cancer and hepatic protective effect. In this study, we investigated polyphenolic extract in L japonica T. using high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS) and its anti-cancer effect on hepatocarcinoma cells. Human HepG2 cell line was treated with various concentrations of polyphenolic extract. Apoptosis was detective by cell morphology, cell cycle analysis and immunoblot analysis. Polyphenolic extract inhibited cell proliferation at 48 h in a dose-dependent manner. Polyphenolic extract affected HepG2 cell viability by inhibiting cell cycle progression at the G2/M transition and inducing apoptosis. Polyphenolic extract also decreased the expression of CDK1, CDC25C, cyclin B1, pro-caspases-3 and -9 and poly ADP ribose polymerase, and affected the levels of mitochondrial apoptotic-related proteins. The phosphorylation of extracellular signal-related kinase 1/2 (ERK 1/2), c-Jun N-terminal kinase (JNK), and p-38 mitogen-activated protein kinases (MAPKs) were increased in HepG2 cells treated with polyphenolic extract, whereas Akt was dephosphorylated. These results indicate that inhibition of PI3K/Akt and activation of MAPKs are pivotal in G2/M cell cycle arrest and apoptosis of human hepatocarcinoma cells mediated by polyphenolic extract. (C) 2012 Elsevier Ltd. All rights reserved.
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