期刊
FOOD AND CHEMICAL TOXICOLOGY
卷 50, 期 8, 页码 2930-2936出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fct.2012.05.033
关键词
Anisomeles indica; Anti-metastasis; Migration and invasion; MMP-9; NF-kappa B/AP-1 signaling
资金
- National Science Council of Taiwan [NSC 98-2320-B-324-001, NSC 100-2811-M-324-001]
Anisomeles indica popularly known in Taiwan as 'yu-chen-tsao' has been traditionally used as an anticancer and anti-inflammatory agent; however, little is known about its anti-metastatic potential. Therefore, we attempted in this study to examine the anti-metastatic potential of A. indica aqueous extract (AI), its isolated compounds apigenin, ovatodiolide, beta-sitosterol and acteoside in 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced human breast adenocarcinoma MCF-7 cells. Among the test agents, crude extract AI and pure compound apigenin potently suppressed the TPA-induced MCF-7 cells migration and invasion. In addition, AI and apigenin time- and dose-dependently down regulated the matrix metalloproteinase (MMP)-9 enzymatic activities and its mRNA expression. Furthermore, AI and apigenin also down regulated the nuclear factor (NF)-kappa B subunit p65, and activator protein (AP)-1 subunit c-Fos proteins expression in nucleus and, transcriptional activity of NF-kappa B and AP-1. This is the first report on the anti-metastatic potential of A. indica that suppressed the cancer cell invasion through the inhibition of MMP-9 enzyme via NF-kappa B/AP-1 signaling. Taken together, our data indicate that A. indica can be considered as a source of new anti-metastatic agent for food and pharmaceutical industries. (C) 2012 Elsevier Ltd. All rights reserved.
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