Review
Chemistry, Applied
Juliana Machado Dutra, J. P. Paula Espitia, Rejane Andrade Batista
Summary: Formononetin is an isoflavone compound that belongs to phytoestrogens and has a broad spectrum of physiological effects with potential applications in various industries. It can be used for product development in food, medicine, cosmetics, and has shown promise in the prevention and treatment of chronic diseases in the medical field, such as cancer, obesity, and neurodegenerative diseases.
Article
Biochemistry & Molecular Biology
Zi-Wen Zhou, Kunmei Ji, Xue-Yan Zhu, Xin-Ying Wu, Ruo-Tong Lin, Chu-Chu Xie, Ze-Lang Cai, Jia-Jie Chen
Summary: In this study, the natural isoflavone formononetin (FNT) was found to inhibit IgE-mediated mast cell activation and reduce allergic inflammation. FNT suppressed the release of inflammatory factors, histamine, and beta-hexosaminidase, and inhibited NF-kappa B and MAPK activity in mast cells. FNT also induced degradation and ubiquitination of the high-affinity IgE receptor, suggesting its potential as a therapeutic target for allergic diseases.
Article
Biochemistry & Molecular Biology
Jinjin Cao, Tingting Li, Ting Liu, Yanhui Zheng, Jiamiao Liu, Qifan Yang, Xuguang Li, Wenbo Lu, Yongju Wei, Wenhong Li
Summary: The spectral difference between formononetin (F) and ononin (FG), two isoflavones, is explained through a study of their fluorescence molecular structure. The fluorescence enhancement of FG in hot alkaline conditions is reported for the first time. F exhibits fluorescence only at pH >4.8 due to deprotonation of 7-OH, while the anionic form of F produces strong and stable fluorescence between pH 9.3 and 12.0. The fluorescence quantum yields of F and FG are measured to be 0.042 and 0.020, respectively. The differences in fluorescence characteristics between F and FG allow for the identification and quantitative analysis of components in Chinese herbal medicine.
Article
Chemistry, Medicinal
Yu Egashira, Tomoka Shimada, Kei Mayumi
Summary: In this study, a method was developed to evaluate the contribution of albumin-mediated hepatic uptake by considering the albumin binding fraction in plasma, leading to accurate prediction of human PK profiles. The findings suggest that the proposed PBPK approach is useful in scientific discussions with regulatory authorities.
JOURNAL OF PHARMACEUTICAL SCIENCES
(2021)
Article
Cell Biology
Xian-liang Zhao, Zhuang-gui Chen, Tian-ci Yang, Ming Jiang, Jie Wang, Zhi-xue Cheng, Man-jun Yang, Jia-xin Zhu, Tian-tuo Zhang, Hui Li, Bo Peng, Xuan-xian Peng
Summary: Exogenous glutamine can enhance the killing effect of antibiotics against multidrug-resistant bacteria, promote the influx of antibiotics, increase intracellular antibiotic concentrations, effectively prevent the development of antibiotic resistance, and provide new insights for the prevention or management of chronic, multidrug-resistant bacterial infections, bacterial persistence, and difficult-to-treat bacterial biofilms.
SCIENCE TRANSLATIONAL MEDICINE
(2021)
Review
Pharmacology & Pharmacy
Riddhi Trivedi, Kalyani Barve
Summary: Anemia, largely caused by iron deficiency, is commonly treated with oral iron supplementation, with intravenous administration also considered. Research focuses on overcoming limitations of iron therapies and improving iron absorption and uptake.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Chemistry, Medicinal
Florian Brandt, Martin Ullrich, Markus Laube, Klaus Kopka, Michael Bachmann, Reik Loeser, Jens Pietzsch, Hans-Juergen Pietzsch, Joerg van den Hoff, Robert Wodtke
Summary: The intentional binding of radioligands to albumin in the context of cancer therapy has potential benefits in enhancing radioactivity uptake into tumor lesions. This study designed clickable albumin binders and applied them to modify a somatostatin receptor ligand for radiopharmacological characterization, providing motivation for further investigation in endoradionuclide therapies.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Cell Biology
Kaiming Cao, Shixuan Li, Yu Wang, Hongze Hu, Sijia Xiang, Qianling Zhang, Yangzhong Liu
Summary: Recently, it has been discovered that bacterial cells contain membrane-less organelles formed by liquid-liquid phase separation of proteins or nucleic acids. In this study, it was found that the bacterial nickel-responsive regulatory protein, NikR, exhibits liquid-liquid phase separation in both solution and cells. Furthermore, disrupting the phase separation promotes the expression of nickel transporter genes, suggesting that the formation of these membrane-less compartments is a regulatory mechanism in bacterial cells.
Article
Microbiology
Fei Yan, Sheng Dong, Ya-Jun Liu, Xingzhe Yao, Chao Chen, Yan Xiao, Edward A. Bayer, Yuval Shoham, Chun You, Qiu Cui, Yingang Feng
Summary: This study clarifies the function and mechanism of substrate specificity of the five putative sugar transporters in Clostridium thermocellum. The results showed that transporter B is the major cellodextrin transporter, while transporter A is the major glucose transporter. The missing ATPase gene of transporter B was identified, and the correlation between transporter B and cellulosome production was revealed. Understanding the mechanism of cellodextrin utilization in C. thermocellum will facilitate the engineering of this strain for industrial applications.
Article
Food Science & Technology
Anne-Sofie Ravn Ballegaard, Jeppe Madura Larsen, Peter Have Rasmussen, Eva Untersmayr, Kirsten Pilegaard, Katrine Lindholm Bogh
Summary: Within the last decade, quinoa seeds have gained popularity as a new food and are now being considered as a suitable option for early introduction in infants. This study aimed to investigate the impact of quinoa on intestinal permeability and inflammation, comparing it with positive controls cholera toxin (CT) and capsaicin. The findings demonstrate that quinoa can increase intestinal permeability and promote compartment-specific protein uptake via mechanisms that may differ from CT and capsaicin.
MOLECULAR NUTRITION & FOOD RESEARCH
(2021)
Article
Chemistry, Applied
Maria Teresa Munoz, Vanessa Maldonado, Wence Herrera, Ana Mutis, Leonardo Bardehle, Cristian Medina, Emilio Hormazabal, Fernando Ortega, Andres Quiroz
Summary: The study aimed to determine the optimal conditions for isoflavone synthase activity through a response surface model and evaluate the formononetin content in Trifolium pratense. The results indicated that the best enzymatic assay condition was at pH 9.1 with an incubation at 34.5 degrees C for 155 minutes, with Precoz-3, Precoz-1, and Superqueli-INIA showing the highest production levels.
NATURAL PRODUCT RESEARCH
(2022)
Article
Chemistry, Multidisciplinary
Jiayi Zhu, Zhipeng Zhao, Huarui Chen, Xinglin Chen, Jinbin Liu
Summary: This study demonstrates that renal-clearable nanomedicines show a more sensitive response to injection dose and have improved tumor-targeting efficiency compared to nonrenal-clearable nanoparticles.
Review
Endocrinology & Metabolism
Ekram Ahmed Chowdhury, Behnam Noorani, Faleh Alqahtani, Aditya Bhalerao, Snehal Raut, Farzane Sivandzade, Luca Cucullo
Summary: This review discusses the significance of the blood-brain barrier in drug delivery and the evolution of different pharmacokinetic techniques in measuring brain uptake and permeability of small molecules. It emphasizes the importance of utilizing the right method to properly assess CNS exposure and acknowledges the progress made while recognizing the need to reduce failures in central nervous system drug development programs.
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
(2021)
Review
Chemistry, Analytical
Behrouz Seyfinejad, Sibel A. Ozkan, Abolghasem Jouyban
Summary: The article discusses the presence of drugs in the bloodstream in both free and bound forms, and how protein binding affects the free concentration of the drug. It highlights the importance of measuring the free concentration of drugs, their protein binding, and the benefits for personalized medicine and therapeutic drug monitoring.
Article
Pharmacology & Pharmacy
Jiahong Xiang, Mengqi Wu, Jianchao Wang, Mengmeng Lin, Mengmeng Sun, Xin Li, Ruijuan Xing, Ran Guo, Jianmin Gu, Tao Lyu, Lei Wang, Xiaowei Shi
Summary: Glytrexate, a novel multitarget folate antagonist developed by our team, shows inhibitory effects on various cancer cell types, especially KB tumor cells. A liquid chromatography-tandem mass spectrometry method was developed and applied to evaluate the stability, plasma protein binding, and pharmacokinetics of glytrexate. Results suggest that glytrexate has potential clinical application prospects as an injection formulation compared to oral administration.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Chemistry, Medicinal
Himalaya Singh, Annaram Thirupathi, Bishwaprava Das, Manojkumar Janni, Renu Kumari, Sandeep Singh, Mamunur Rashid, Mohammad Wahajuddin, Musuvathi Motilal Balamurali, Kumaravelu Jagavelu, Saravanan Peruncheralathan
Summary: The study demonstrates that 2,3-ACBTs inhibit angiogenesis by regulating the Akt/Src signaling pathway and disrupting cytoskeletal filaments of endothelial cells.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Guru R. Valicherla, Roshan A. Katekar, Shailesh Dadge, Mohammed Riyazuddin, Anees A. Syed, Sandeep K. Singh, Athar Husain, Muhammad Wahajuddin, Jiaur R. Gayen
Summary: This study investigates the pharmacokinetic properties of PSTi8 in Sprague Dawley rats for the first time. The results show that PSTi8 has high solubility and plasma protein binding, stability, and good bioavailability in rats. These findings support the development of PSTi8 as an antidiabetic agent.
Article
Nanoscience & Nanotechnology
Manju Sharma, Virendra Tiwari, Swati Chaturvedi, Muhammad Wahajuddin, Shubha Shukla, Jiban Jyoti Panda
Summary: This study developed self-fluorescent nanoparticles as a therapeutic system for Alzheimer's disease (AD), showing the ability to inhibit and disrupt the amyloid fibrils associated with AD. These nanoparticles were nontoxic to neural cells and protected neurons from cytotoxicity. Animal model experiments demonstrated that the nanoparticles improved memory in AD model rats. Overall, the nanoparticles showed promising potential as nanotheranostic agents for AD therapy.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Food Science & Technology
Manisha Bhateria, Kajal Karsauliya, Ashish Kumar Sonker, C. Yahavi, Sheelendra Pratap Singh
Summary: This study investigated the toxicokinetic properties of ENNA in vitro and in vivo. The results showed the metabolism and clearance process of ENNA in different species and provided various kinetic parameters. These findings are significant for designing and interpreting toxicological studies and predicting the risk assessment of ENNA in humans.
FOOD AND CHEMICAL TOXICOLOGY
(2022)
Article
Biochemical Research Methods
Shashyendra Singh Gautam, Raghwendra Pratap Singh, Kajal Karsauliya, Ashish Kumar Sonker, Panga Jaipal Reddy, Divya Mehrotra, Sameer Gupta, Sudhir Singh, Rashmi Kumar, Sheelendra Pratap Singh
Summary: Mass spectrometry-based label-free proteomics was used to profile the plasma proteome of Indian OSCC patients. The study identified potential biomarkers for OSCC and revealed their involvement in disease progression through pathway and network analysis.
JOURNAL OF PROTEOMICS
(2022)
Article
Biochemistry & Molecular Biology
Shubhangini Tiwari, Parul Gupta, Abhishek Singh, Swati Chaturvedi, M. Wahajuddin, Amit Mishra, Sarika Singh
Summary: This study investigated the therapeutic effects of the drug 4-phenylbutyric acid (4PBA) in Parkinson's disease (PD) and found that it can protect neurons, reduce oxidative stress and calcium levels, inhibit neuronal apoptosis, restore neuronal morphology, and inhibit astrocyte activation.
NEUROCHEMICAL RESEARCH
(2022)
Article
Biology
Farogh Ahsan, Tarique Mahmood, Tanveer A. Wani, Seema Zargar, Mohammed Haris Siddiqui, Shazia Usmani, Arshiya Shamim, Muhammad Wahajuddin
Summary: The research evaluated the effect of sericin on myocardial necrosis and hypertrophy, and found that sericin has significant cardioprotective effects.
Article
Oncology
Swati Chaturvedi, Zaiba Naseem, Sherif F. El-Khamisy, Muhammad Wahajuddin
Summary: This article discusses the application of nanomedicines in aging related disorders and the modulation of signaling molecules in the inflammasome cascade by nanomedicines.
SEMINARS IN CANCER BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Rafat Ali, Rohil Hameed, Divya Chauhan, Shantanu Sen, Muhammad Wahajuddin, Aamir Nazir, Sandeep Verma
Summary: This study demonstrates the design, synthesis, and biological activity of peptide conjugates that release H2S and possess anti-Alzheimer's disease properties. The conjugates target multiple factors involved in the progression of AD, including inhibition of aggregation, reduction of oxidative stress, and increased levels of ACh.
ACS CHEMICAL NEUROSCIENCE
(2022)
Review
Biochemistry & Molecular Biology
Yuliya Linhares, Alexander Kaganski, Christian Agyare, Isil A. Kurnaz, Vidushi Neergheen, Bartlomiej Kolodziejczyk, Monika Kedra, Muhammad Wahajuddin, Lahcen El-Youssf, Thomas Edison dela Cruz, Yusuf Baran, Milica Pesic, Uttam Shrestha, Rigers Bakiu, Pierre-Marie Allard, Stanislav Rybtsov, Myrtani Pieri, Velia Siciliano, Yensi Flores Bueso
Summary: Biodiversity is crucial for the robustness, stability, and re-silience of ecosystems, which in turn provide essential services for human survival. However, human activity is causing a rapid decline in biodiversity, posing a threat to the planet's life-support systems. This article emphasizes the importance of biodiversity for human health, particularly in the field of biomedicine, and presents approaches to promote biodiversity conservation.
TRENDS IN MOLECULAR MEDICINE
(2023)
Article
Pharmacology & Pharmacy
Virendra Tiwari, Jitendra Singh, Priya Tiwari, Swati Chaturvedi, Shivangi Gupta, Akanksha Mishra, Sonu Singh, Muhammad Wahajuddin, Kashif Hanif, Shubha Shukla
Summary: In this study, the role of the ACE2/Ang-(1-7)/Mas receptor axis in Alzheimer's disease (AD) was investigated using various methods. The results showed that activation of this axis could improve cognitive impairment and amyloid deposition in an AD-like rat model. These findings suggest the potential involvement of the ACE2/Ang-(1-7)/Mas axis in the pathophysiology of AD by regulating inflammation and cognitive functions.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Pavan K. Yadav, Ravi Saklani, Amrendra K. Tiwari, Saurabh Verma, Rafquat Rana, Divya Chauhan, Pooja Yadav, Keerti Mishra, Ashwini S. Kedar, Navodayam Kalleti, Jiaur R. Gayen, Muhammad Wahajuddin, Srikanta K. Rath, Madhav N. Mugale, Kalyan Mitra, Deepak Sharma, Manish K. Chourasia
Summary: The aim of this study was to develop a nanoemulsion (NE) for the effective delivery of paclitaxel (PTX) in breast cancer therapy. The TPP-TPGS(1000)-PTX-NE showed enhanced cellular uptake, mitochondrial membrane depolarization, and cell cycle arrest compared to free-PTX treatment. In vivo studies in tumor-bearing mice demonstrated superior performance of TPP-TPGS(1000)-PTX-NE in pharmacokinetics, biodistribution, and live imaging, with histological and survival investigations confirming its non-toxicity and potential for breast cancer treatment.
Article
Chemistry, Medicinal
Mohammad Shariq, Tarique Mahmood, Poonam Kushwaha, Saba Parveen, Arshiya Shamim, Farogh Ahsan, Tanveer A. Wani, Seema Zargar, Rufaida Wasim, Muhammad Wahajuddin
Summary: Nanotechnology is an effective tool for drug delivery in the treatment of Coronary heart disease (CHD). A combination nanoformulation of sericin and carvedilol has shown potential in protecting the heart. Chitosan nanoparticles were prepared and evaluated for their cardioprotective properties in doxorubicin-induced cardiotoxicity. The developed nanoparticle formulation was effective in reducing myocardial damage and improving cardiac markers.
Article
Biochemical Research Methods
Pavan K. Yadav, Amrendra K. Tiwari, Ravi Saklani, Divya Chauhan, Rafquat Rana, Pooja Yadav, Keerti Mishra, Ashwini S. Kedar, Wahajuddin, Jiaur R. Gayen, Manish K. Chourasia
Summary: A novel HPLC method has been developed and validated for simultaneous estimation of paclitaxel and baicalein. The method showed a wide linear range and low limits of quantification. According to international guidelines, the method is precise and accurate, suitable for formulation development and bioanalytical studies.
Article
Biochemistry & Molecular Biology
Anuradha Seth, Anirban Ghoshal, Varun Dewaker, Ankita Rani, Sangh Priya Singh, Mukul Dutta, Shivani Katiyar, Sandeep Kumar Singh, Mamunur Rashid, Muhammad Wahajuddin, Susanta Kar, Ajay Kumar Srivastava
Summary: A series of uniquely functionalized 2,3-dihydro-1H-pyrrolo[3,4-b]quinolin-1-one derivatives were synthesized using a post-Ugi modification strategy. Compound 5m showed potential antileishmanial activity and demonstrated inhibition of parasite burden in liver and spleen in vivo. The in vitro pharmacokinetic study confirmed the stability of compound 5m and it showed no toxicity in in silico predictions.
RSC MEDICINAL CHEMISTRY
(2022)
