Article
Chemistry, Analytical
Jinxin He, Yan Zheng, Xiaorong Chen, Na Li, Fang Tang, Nairui Huo, Jianzhong Wang, Shaopeng Gu
Summary: A rabbit IgG-based ELISA was developed for the sensitive and specific detection of MBZ residues in food samples, showing promise for routine screening analysis.
ANALYTICAL METHODS
(2021)
Article
Chemistry, Applied
Chia-Lin Tsai, I-Ning Chen, Yi-Tien Chen
Summary: Mollusks, a major allergenic food, must be declared on food products to reduce the risk of allergies. However, there is currently no reliable immunoassay for detecting edible mollusks. In this study, a sandwich enzyme-linked immunosorbent assay (sELISA) was developed to detect 32 edible mollusk species without cross-reaction with non-mollusk species. The assay proved effective in detecting various cooked and raw mollusk samples, as well as commercial mollusk products.
Article
Agriculture, Multidisciplinary
Yifan Liang, Yuyao Zeng, Lin Luo, Zhenlin Xu, Yudong Shen, Hong Wang, Bruce D. Hammock
Summary: A specific nanobody Nb-7E against acrylamide was isolated and confirmed to be able to detect acrylamide. An ic-ELISA and an ECIA were developed for acrylamide determination, with ECIA showing lower limit of detection and wider linear detection range.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Hyeokjung Kim, Hyeran Noh
Summary: This research presents a novel signal amplification method for immunoassay that utilizes spatial concentration of gold nanoparticles on a cellulose-based plate to enhance color signals. The method shows potential benefits including versatility, simplicity, low cost, and the ability to operate multiple plates simultaneously.
Article
Chemistry, Applied
Yahui Guo, Panting Sang, Gang Lu, Xue Yang, Yunfei Xie, Zhigang Hu, He Qian, Weirong Yao
Summary: An artificial deoxyribozyme was used as a substitute for horseradish peroxidase (or alkaline phosphatase) in ELISA for generating amplified signals. The feasibility of the proposed deoxyribozyme-based ELISA (DLISA) was demonstrated in the detection of a forbidden veterinary drug, chloramphenicol. With a detection limit of 0.1 ng/L and a wide linear range from 0.000001 μg/mL to 1.0 μg/mL, DLISA showed excellent practical performances. The easy connection of antibody and DNAzyme through gold nanoparticles in DLISA made it worth popularizing.
Article
Immunology
Randy M. Stalter, Jared M. Baeten, Deborah Donnell, Matthew A. Spinelli, David Glidden, Warren C. Rodrigues, Guohong Wang, Michael Vincent, Nelly Mugo, Andrew Mujugira, Mark Marzinke, Craig Hendrix, Monica Gandhi
Summary: In order to support PrEP adherence for HIV prevention, new tools are needed, including those that offer real-time feedback. In a large completed PrEP trial, a novel immunoassay measuring adequate urine tenofovir levels predicted HIV protection and demonstrated good sensitivity and specificity for detectable plasma tenofovir.
CLINICAL INFECTIOUS DISEASES
(2021)
Article
Biochemistry & Molecular Biology
Shreyasee Das, Nele Dewit, Dirk Jacobs, Yolande A. L. Pijnenburg, Sjors G. J. G. In't Veld, Salome Coppens, Milena Quaglia, Christophe Hirtz, Charlotte E. Teunissen, Eugeen Vanmechelen
Summary: This study describes a novel Nf-L enzyme-linked immunosorbent assay (ELISA) with well-defined specificity and epitopes, which can accurately measure Nf-L analytes in cerebrospinal fluid and discriminate different diseases in clinical diagnosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Engineering, Chemical
Martina Zangheri, Maria Maddalena Calabretta, Donato Calabria, Jessica Fiori, Massimo Guardigli, Elisa Michelini, Sonia Melandri, Assimo Maris, Mara Mirasoli, Luca Evangelisti
Summary: Cosmetics analysis is a rapidly expanding field that requires compliance with strict regulations in each country. Research focuses on finding innovative techniques for the rapid, inexpensive, and sensitive detection of target analytes.
Article
Infectious Diseases
Alexander Ecke, Rudolf J. Schneider
Summary: Pharmaceutical contamination of water can lead to antimicrobial resistance, necessitating fast and cost-effective analytical methods. Antibody-based techniques, like ELISA, can aid in analyzing pharmaceutical contamination but may have drawbacks. Hydrolysis of beta-lactam antibiotics is shown to significantly reduce detection limits in immunochemical analysis in this study.
Article
Chemistry, Analytical
Binh V. Vu, Kristen Brosamer, Naiyah McDaniel, Katerina Kourentzi, Richard C. Willson, Harshica Fernando
Summary: Glow enzyme-linked immunosorbent assay (glow ELISA) utilizes inexpensive glow stick reagents to chemically excite fluorescent reporters, eliminating the need for complex optics and equipment, while achieving excellent detection limits and portability.
Article
Chemistry, Analytical
Anna Raysyan, Sandro D. Zwigart, Sergei A. Eremin, Rudolf J. Schneider
Summary: BPA, commonly used in polymer-based materials, has detrimental effects on thyroid gland and reproductive health. Fluorescence polarization immunoassay (FPIA) is a cost-effective and efficient method that offers high specificity and sensitivity for detecting BPA. New tracer molecules were designed and assessed using ELISA, resulting in a highly sensitive assay with a detection limit of 0.05 g/L.
Article
Chemistry, Analytical
Honglin Yan, Xiaoqian Tang, Xiaohan Liu, Yating Zheng, Minhui Zhang, Yueju Zhao, Qi Zhang
Summary: In this study, we explored the feasibility of developing immunoassay technology with a linear carrier to create a simpler and cheaper rapid immunoassay. Aflatoxins were selected as the focus of research due to their highly toxic and carcinogenic nature. We found that mercerized cotton and Dyneema line showed high-signal values and relatively low background noise, making them suitable for further development and competitive immunoassay research.
Article
Medical Laboratory Technology
Payam Kabiri, Ralf Weiskirchen, Josef van Helden
Summary: False laboratory findings due to biotin supplementation have raised concerns about the safety of immunoassays. Two experiments suggest that biotin interference in laboratory findings is probable, with falsely elevated test results occurring more frequently than falsely low results. Laboratories, medical staff, and patients must be aware of the potential for biotin interference, and further review of interference suppression methods may be necessary for test manufacturers like Roche Diagnostics.
JOURNAL OF CLINICAL LABORATORY ANALYSIS
(2021)
Article
Chemistry, Analytical
Jie Fei, Jiaqun Jiang, Yuchen Bai, Weilin Wu, Xiangjun Zhao, Wenbo Yu, Kai Wen, Xuezhi Yu, Jianzhong Shen, Zhanhui Wang
Summary: This study demonstrates the importance of careful hapten design and extensive antibody screening in the development of sandwich immunoassays for small molecules, using ABM as an example. Through combinatorial associations of antibody pairs, a sandwich immunoassay for ABM was successfully established.
ANALYTICAL CHEMISTRY
(2023)
Article
Food Science & Technology
Kyoko Noda, Yuki Hirakawa, Tomomi Nishino, Ritsuto Sekizuka, Marin Kishimoto, Tomohiro Furukawa, Sakiko Sawane, Ayu Matsunaga, Naoki Kobayashi, Kazutoshi Sugita, Kenji Oonaka, Hiroko Kawakami, Yuji Otsuka, Tetsuya Yamamoto, Toshihiro Yamamoto, Taku Yoshiya, Maiko Watanabe, Machiko Saka, Keiko Momma, Masayo Kushiro, Shiro Miyake
Summary: Nivalenol (NIV) is a mycotoxin that is more toxic than deoxynivalenol, and specific antibodies against it were successfully generated for the first time in this study. These monoclonal antibodies exhibited high reactivity towards NIV and 15-acetyl-NIV, indicating their potential use in immunoassays for monitoring NIV contamination in grains.