Article
Infectious Diseases
Ola A. Abd El-Rahman, Fatma Rasslan, Safaa S. Hassan, Hossam M. Ashour, Reham Wasfi
Summary: MDR Acinetobacter baumannii is a critical pathogen in healthcare-associated infections, with its ability to form biofilms and transfer antibiotic resistance genes. The role of the RND efflux pump in biofilm formation was studied, and while it did not appear to be pivotal, the expression varied among different strains.
Article
Infectious Diseases
Christina Meyer, Kai Lucassen, Stefanie Gerson, Kyriaki Xanthopoulou, Thorsten Wille, Harald Seifert, Paul G. Higgins
Summary: This study investigated the impact of differential expression levels of RND-type efflux pumps AdeABC and AdeIJK on the susceptibility of multidrug-resistant Acinetobacter baumannii to commonly used biocides. The results showed that overexpression of AdeABC correlated with greater survival after exposure to certain biocides. These findings highlight the role of efflux pumps in the survival of A. baumannii when exposed to residual concentrations of biocides.
Article
Microbiology
K. Lucassen, K. Xanthopoulou, J. Wille, T. Wille, Y. Wen, X. Hua, H. Seifert, P. G. Higgins
Summary: The amino acid substitutions D26N (AdeR) and T156M (AdeS) significantly increase adeB expression and efflux activity, leading to multidrug resistance, while the D21V (AdeR) substitution has no effect. These findings suggest the importance of these substitutions in interfering with efflux activity to restore antimicrobial susceptibility.
Article
Biochemistry & Molecular Biology
Privita Verma, Monalisa Tiwari, Vishvanath Tiwari
Summary: The study investigated various efflux pumps present in carbapenem-resistant Acinetobacter baumannii (CRAB) and a potential efflux pump inhibitor. Molecular mechanism analysis showed that naringin dihydrochalcone-capped silver nanoparticles have multiple mechanisms of action, inhibiting efflux activity, generating reactive oxygen and nitrogen species, altering the electrochemical gradient, and CRAB did not develop resistance against them after several generations.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Infectious Diseases
Yanpeng Zhang, Bing Fan, Yong Luo, Zhiyuan Tao, Yongbo Nie, Yongtao Wang, Fanglin Ding, Yanwu Li, Dayong Gu
Summary: Carbapenem non-susceptible A. baumannii (CnSAB) strains showed increased distribution of carbapenemase genes and overexpression of RND family efflux pumps, leading to stronger antibiotic resistance, while carbapenem susceptible A. baumannii (CSAB) strains exhibited higher biofilm formation potential to survive antibiotic stress.
BMC INFECTIOUS DISEASES
(2021)
Article
Microbiology
Sonia Prieto Martin Gil, Ana Tajuelo, Mireia Lopez-Siles, Michael J. McConnell
Summary: This study revealed that subinhibitory concentrations of clinically-relevant antibiotics and disinfectants have variable effects on the promoter activity of RND family members in Acinetobacter baumannii, depending on the strain, compound tested, and growth phase. These findings suggest important implications for the treatment of A. baumannii infections.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Maoge Zang, Hugo MacDermott-Opeskin, Felise G. Adams, Varsha Naidu, Jack K. Waters, Ashley B. Carey, Alex Ashenden, Kimberley T. McLean, Erin B. Brazel, Jhih-Hang Jiang, Alessandra Panizza, Claudia Trappetti, James C. Paton, Anton Y. Peleg, Ingo Koper, Ian T. Paulsen, Karl A. Hassan, Megan L. O'Mara, Bart A. Eijkelkamp
Summary: This study explores the impact of host-derived polyunsaturated fatty acids (PUFAs) on Acinetobacter baumannii's susceptibility to antibiotics, specifically by affecting AdeB-mediated antibiotic resistance. The findings suggest that A. baumannii's unconditional desire for fatty acids may present a possible weakness in its multidrug resistance capacity.
Article
Biochemistry & Molecular Biology
Alicja Sloczynska, Matthew E. Wand, Lucy J. Bock, Stefan Tyski, Agnieszka E. Laudy
Summary: This study investigates the contribution of efflux mechanism to carbapenem resistance in A. baumannii clinical strains. Efflux pump inhibitors reduced carbapenem resistance in some isolates. The presence of AbaR25 resistance island with ISAba1 element was found to be involved in carbapenem resistance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Jeongwoo Jo, Kwan Soo Ko
Summary: The study on Acinetobacter baumannii isolates from South Korea revealed a high prevalence of tigecycline heteroresistance, mainly attributed to the overexpression of AdeABC efflux pumps due to ISAba1 insertion in adeS. Resistance was found to be unstable in an antibiotic-free environment, with potential for reversion to susceptibility through additional mutations.
MICROBIOLOGY SPECTRUM
(2021)
Article
Infectious Diseases
Nayeong Kim, Joo-Hee Son, Kyeongmin Kim, Hyo-Jeong Kim, Minsang Shin, Je-Chul Lee
Summary: The study found that DksA plays a regulatory role in the antimicrobial susceptibility of Acinetobacter baumannii, with increment dksA mutant strains showing increased susceptibility to quinolones and tetracyclines but decreased susceptibility to aminoglycosides, and upregulation of efflux pump gene expression. In contrast, (p)ppGpp-deficient mutants downregulate efflux pump gene expression, indicating that (p)ppGpp and DksA have opposite roles in modulating antimicrobial susceptibility via efflux pump gene regulation in A. baumannii.
Article
Microbiology
Morgane Choquet, Elodie Lohou, Etienne Pair, Pascal Sonnet, Catherine Mullie
Summary: The study evaluated the overexpression of efflux pumps in ciprofloxacin-resistant Acinetobacter baumannii strains, with most genes belonging to the RND family. Synthesis of 1-(1-naphthylmethyl)piperazine analogs as potential efflux pump inhibitors showed that quinoline and pyridine analogs were more effective, with stereochemistry playing a key role in inhibitory activity.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Microbiology
Maoge Zang, Felise G. Adams, Karl A. Hassan, Bart A. Eijkelkamp
Summary: Acinetobacter baumannii has become an urgent threat to healthcare systems, with multidrug resistance closely linked to the plasticity of its genome. Host fatty acids play a critical role in the development of resistance to antibiotics in A. baumannii, highlighting the potential for novel approaches in tackling antimicrobial resistance.
MICROBIOLOGY SPECTRUM
(2021)
Article
Infectious Diseases
Jenifer Cuesta Bernal, Jasmin El-Delik, Stephan Goettig, Klaas M. Pos
Summary: The study found that the Gram-negative bacterium AcrD efflux pump confers resistance to various antibiotics, including some previously unreported substrates. Substitution analyses of AcrD revealed key amino acid residues involved in drug binding and transport.
Article
Biotechnology & Applied Microbiology
Ebrahim Rafiei, Milad Shahini Shams Abadi, Behnam Zamanzad, Abolfazl Gholipour
Summary: The study found that multidrug-resistant A. baumannii isolates are resistant to Ciprofloxacin and Trimethoprim-sulfamethoxazole but more sensitive to Tigecycline. The frequency of efflux pump genes in these resistant strains is high, indicating that efflux pumps are one of the antibiotic resistance factors of A. baumannii.
Article
Infectious Diseases
Esther Boyer, Jean Dessolin, Margaux Lustig, Marion Decossas, Gilles Phan, Quentin Cece, Gregory Durand, Veronique Dubois, Joris Sansen, Jean-Christophe Taveau, Isabelle Broutin, Laetitia Daury, Olivier Lambert
Summary: This study reveals the Q93R MexA mutation and the OprM C-terminal peptide as molecular determinants modulating the assembly process efficacy with cognate and non-cognate OMFs, even though they are outside the interfacial contact. It provides insights into how OMF selectivity operates during the formation of the tripartite complex.
Article
Cardiac & Cardiovascular Systems
Jolanta Malyszko, Anna Tomaszuk-Kazberuk, Konrad Dobrzycki, Hanna Bachorzewska-Gajewska, Marcin Zorawski, Ewa Koc-Zorawska, Grazyna Kobus, Slawomir Dobrzycki
Summary: This study revealed that vitamin D deficiency is common in patients with CHF, with valvular disease associated with the most severe deficiency. Anemia prevalence in CHF due to coronary heart disease may be linked to the wider use of certain medications. Heart and kidney function are predictors of vitamin D level in CHF patients.
CARDIOLOGY JOURNAL
(2021)
Review
Environmental Sciences
Anna Buklaha, Anna Wieczorek, Piotr Majewski, Dominika Iwaniuk, Pawel Sacha, Elzbieta Tryniszewska, Piotr Wieczorek
Summary: Fogging technology, using fumigants such as hydrogen peroxide and peracetic acid, is widely used in various industries for efficient and safe disinfection. It has become especially significant in the twenty-first century due to the need to combat and prevent the spread of the coronavirus. Fumigation allows for the disinfection of hard-to-clean areas and is applicable in medical, pharmaceutical, and transportation fields, among others.
ANNALS OF AGRICULTURAL AND ENVIRONMENTAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Karolina Woziwodzka, Jolanta Malyszko, Ewa Koc-Zorawska, Marcin Zorawski, Paulina Dumnicka, Artur Jurczyszyn, Krzysztof Batko, Paulina Mazur, Malgorzata Banaszkiewicz, Marcin Krzanowski, Paulina Golasa, Jacek A. Malyszko, Ryszard Drozdz, Katarzyna Krzanowska
Summary: This study provides the first evidence that elevated serum transgelin is negatively associated with glomerular filtration in multiple myeloma patients and predicts a decline in renal function over long-term follow-up.
Article
Microbiology
Piotr Majewski, Anna Gutowska, David G. E. Smith, Tomasz Hauschild, Paulina Majewska, Tomasz Hryszko, Dominika Gizycka, Boguslaw Kedra, Jan Kochanowicz, Jerzy Glowinski, Justyna Drewnowska, Izabela Swiecicka, Pawel T. Sacha, Piotr Wieczorek, Dominika Iwaniuk, Anetta Sulewska, Radoslaw Charkiewicz, Katarzyna Makarewicz, Agnieszka Zebrowska, Slawomir Czaban, Piotr Radziwon, Jacek Niklinski, Elzbieta A. Tryniszewska
Summary: The study identified six extraintestinal Escherichia coli isolates that produce the mcr-1.1 gene, leading to colistin resistance. These strains also possess multiple virulence factors. The IncX4 plasmids carrying mcr genes were found to be disseminated among E. coli isolates and may facilitate the spread of colistin resistance genes between different clones.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Oncology
Anetta Sulewska, Jacek Niklinski, Radoslaw Charkiewicz, Piotr Karabowicz, Przemyslaw Biecek, Hubert Baniecki, Oksana Kowalczuk, Miroslaw Kozlowski, Patrycja Modzelewska, Piotr Majewski, Elzbieta Tryniszewska, Joanna Reszec, Zofia Dzieciol-Anikiej, Cezary Piwkowski, Robert Gryczka, Rodryg Ramlau
Summary: This study aimed to evaluate the diagnostic value of 14 lncRNAs in the early stages of non-small-cell lung cancer (NSCLC). The results showed that these lncRNAs can accurately distinguish cancerous from noncancerous lung tissues and NSCLC subtypes. The study suggests that these lncRNAs could be a promising tool to support the histopathological diagnosis of NSCLC.
Article
Immunology
Malgorzata Kepska-Dzilinska, Inga Chomicka, Ewa Karakulska-Prystupiuk, Agnieszka Tomaszewska, Grzegorz Wladyslaw Basak, Marcin Zorawski, Jolanta Malyszko
Summary: The aim of this study was to evaluate biomarkers of kidney injury in patients undergoing hematopoietic stem cell transplant. The results showed evidence of kidney injury in patients with normal or near normal kidney function.
TRANSPLANTATION PROCEEDINGS
(2022)
Article
Public, Environmental & Occupational Health
Anna Buklaha, Anna Wieczorek, Ewelina Kruszewska, Piotr Majewski, Dominika Iwaniuk, Pawel Sacha, Elzbieta Tryniszewska, Piotr Wieczorek
Summary: This study aims to test different disinfection agents for automobile cabins and compare their effectiveness in disinfecting car air conditioning and cabin surfaces. The results show that certain disinfectants can be successfully used for disinfection and significantly reduce the amount of microorganisms, improving the quality of cabin air and surfaces.
FRONTIERS IN PUBLIC HEALTH
(2022)
Article
Medicine, General & Internal
Irena Glowinska, Barbara Labij-Reduta, Jerzy Juzwiuk, Magdalena Lukaszewicz, Adam Pietruczuk, Agata Poplawska, Anna Daniluk-Jamro, Katarzyna Kakareko, Alicja Rydzewska-Rosolowska, Beata Naumnik, Ewa Koc-Zorawska, Marcin Zorawski, Tomasz Hryszko
Summary: COVID-19 has significantly impacted patients with end stage renal disease. This study investigated the longevity and modulating factors of humoral response after SARS-CoV-2 vaccination among dialyzed patients. The results showed that obesity increased the risk of antibody waning, while previous COVID-19 infection enhanced the longevity of the humoral response. Vaccine efficacy may also be influenced by previous infection and vaccine type.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Joanna Kacperczyk-Bartnik, Pawel Bartnik, Ksawery Golawski, Janusz Sierdzinski, Grzegorz Manka, Mariusz Kiecka, Michal Lipa, Damian Warzecha, Robert Spaczynski, Piotr Piekarski, Beata Banaszewska, Artur Jakimiuk, Tadeusz Issat, Wojciech Rokita, Jakub Mlodawski, Maria Szubert, Piotr Sieroszewski, Grzegorz Raba, Kamil Szczupak, Tomasz Kluz, Marek Kluza, Krzysztof Czajkowski, Miroslaw Wielgos, Ewa Koc-Zorawska, Marcin Zorawski, Piotr Laudanski
Summary: This study evaluated the levels of PARP in plasma and peritoneal fluid of patients with and without endometriosis. The results showed no significant differences in PARP concentrations between patients with and without endometriosis. However, a higher plasma PARP concentration was associated with a history of infertility.
Article
Biochemistry & Molecular Biology
Pawel Bartnik, Joanna Kacperczyk-Bartnik, Ksawery Golawski, Janusz Sierdzinski, Grzegorz Manka, Mariusz Kiecka, Michal Lipa, Damian Warzecha, Robert Spaczynski, Piotr Piekarski, Beata Banaszewska, Artur J. Jakimiuk, Tadeusz Issat, Wojciech Rokita, Jakub Mlodawski, Maria Szubert, Piotr Sieroszewski, Grzegorz Raba, Kamil Szczupak, Tomasz Kluz, Marek Kluza, Krzysztof Czajkowski, Miroslaw Wielgos, Ewa Koc-Zorawska, Marcin Zorawski, Piotr Laudanski
Summary: ZEB1 and ZEB2, as transcription factors, regulate epithelial-mesenchymal transformation (EMT). However, they do not seem to have significant value in the initial diagnosis of endometriosis as single markers. Elevated plasma ZEB2 levels in infertile patients might indicate a role of EMT dysregulation in the pathogenesis of adverse fertility outcomes.
Article
Biochemistry & Molecular Biology
Zuzanna Zielinska, Lukasz Oldak, Joanna Kacperczyk-Bartnik, Ewa Koc-Zorawska, Marcin Zorawski, Piotr Laudanski, Ewa Gorodkiewicz
Summary: A biosensor was developed to quantify PARP-1 in body fluids. The biosensor showed a linear response range and good precision, accuracy, and recovery. Validation was done using SPRi biosensors and ELISA tests, showing a close correlation coefficient of 1 for PARP-1 determination in plasma samples from endometriosis patients.
Meeting Abstract
Transplantation
Krzysztof Batko, Ewa Koc- Zorawska, Marcin Zorawski, Paulina Dumnicka, Malgorzata Banaszkiewicz, Karolina Woziwodzka, Marcin Krzanowski, Paulina Golasa, Anna Kurnik, Agnieszka Panek, Jacek Malyszko, Jolanta Malyszko, Katarzyna Krzanowska
NEPHROLOGY DIALYSIS TRANSPLANTATION
(2022)
Article
Medicine, General & Internal
Malgorzata Banaszkiewicz, Jolanta Malyszko, Krzysztof Batko, Ewa Koc-Zorawska, Marcin Zorawski, Paulina Dumnicka, Artur Jurczyszyn, Karolina Woziwodzka, Aleksandra Maleszka, Marcin Krzanowski, Andrzej Krasniak, Ryszard Drozdz, Katarzyna Krzanowska
Summary: This study aims to evaluate the biomarkers of iron turnover in patients with multiple myeloma and their relationship with anemia, iron status, inflammation, renal impairment, and disease burden. The results suggest that hepcidin-25 is involved in anemia in multiple myeloma and its concentrations are not affected by kidney impairment. Moreover, serum hepcidin-25 may be an early predictor of survival in this disease, independent of hemoglobin concentration.
MEDICINA-LITHUANIA
(2022)
Article
Medicine, General & Internal
Karolina Woziwodzka, Jolanta Malyszko, Ewa Koc-Zorawska, Marcin Zorawski, Paulina Dumnicka, Artur Jurczyszyn, Krzysztof Batko, Paulina Mazur, Malgorzata Banaszkiewicz, Marcin Krzanowski, Paulina Golasa, Jacek A. Malyszko, Ryszard Drozdz, Katarzyna Krzanowska
Summary: Urinary IGFBP-7 and NGAL monomer may be potential markers of tubular renal damage in MM patients, showing associations with renal function, disease stage, and other parameters.
MEDICINA-LITHUANIA
(2021)