Article
Biotechnology & Applied Microbiology
Teng-Kuei Huang, Brittney Armstrong, Patrick Schindele, Holger Puchta
Summary: Nicotiana tabacum, a non-food herb, shows potential as a bio-factory for producing medicines, vaccines, or valuable metabolites, but improving genetic tools for targeted genome modifications is crucial. By replacing SpCas9 with other CRISPR/Cas-based nucleases like SaCas9 and ttLbCas12a, targeting the tobacco SuRB gene showed improved efficiencies, suggesting ttLbCas12a as a superior alternative for gene editing in tobacco and other crops.
PLANT BIOTECHNOLOGY JOURNAL
(2021)
Article
Plant Sciences
Niels Tol, Robin Schendel, Alex Bos, Maartje Kregten, Sylvia Pater, Paul J. J. Hooykaas, Marcel Tijsterman
Summary: Agrobacterium tumefaciens-mediated transformation is the preferred tool for generating transgenic plants, but gene targeting (GT) events occur at low frequency and are often accompanied by random integration events. The functionality of polymerase theta (Pol theta) affects the biology of GT in plants, with its deficiency preventing T-DNA integration and ectopic GT events. Insights obtained from this study can be used for developing strategies to achieve high-quality GT events in crop plants.
Article
Biochemistry & Molecular Biology
Paul A. P. Atkins, Maria Elena S. Gamo, Daniel F. Voytas
Summary: Researchers overcame the limitations posed by donor molecules in gene targeting events by utilizing ONAS technology and developing the PANGEA bioinformatic pipeline, allowing them to collect a large number of plant somatic GT events and conversion tracts in a short period of time.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Plant Sciences
Irene Guzman-Benito, Natalia Patricia Achkar, Nicolas G. Bologna, Robertas Ursache
Summary: This paper reviews recent advances in CRISPR/Cas-mediated gene targeting in plants, discusses their potential applications, and suggests further improvements to achieve higher efficiency.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Editorial Material
Medicine, Research & Experimental
Jonas Schwickert, Franziska M. Zickgraf, Martin R. Sprick
Summary: Ovarian cancer, especially high-grade serous ovarian cancer (HGSOC), has the worst prognosis among all gynecological cancers. RAD51 expression is proposed as a biomarker of platinum resistance in HGSOC patients, which can help in selecting effective therapies for therapy-resistant patients.
EMBO MOLECULAR MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Thomas Bachelot, Thomas Filleron, Ivan Bieche, Monica Arnedos, Mario Campone, Florence Dalenc, Florence Coussy, Marie-Paule Sablin, Marc Debled, Claudia Lefeuvre-Plesse, Anthony Goncalves, Marie-Ange Mouret Reynier, William Jacot, Benoit You, Philippe Barthelemy, Benjamin Verret, Nicolas Isambert, Xavier Tchiknavorian, Christelle Levy, Jean-Christophe Thery, Tifenn L'Haridon, Jean-Marc Ferrero, Alice Mege, Francesco Del Piano, Etienne Rouleau, Alicia Tran-Dien, Julien Adam, Amelie Lusque, Marta Jimenez, Alexandra Jacquet, Ingrid Garberis, Fabrice Andre
Summary: The study found that single-agent antibodies against PD-L1 as maintenance therapy did not significantly benefit the overall population with metastatic breast cancer, but showed improved overall survival in patients with triple-negative breast cancer. Durvalumab may have better sensitivity in patients with CD274 amplification. Analysis of lymphocyte infiltration and homologous recombination deficiency did not predict sensitivity to durvalumab, warranting further research. Maintenance chemotherapy was more effective than durvalumab in patients with hormone receptor-positive and Her2-negative disease.
Article
Biochemistry & Molecular Biology
Chinnadurai Mani, Kaushlendra Tripathi, Tasmin R. Omy, Mark Reedy, Upender Manne, Komaraiah Palle
Summary: Inhibition of GLI1 in TNBC cells downregulates FANCD2 expression and induces HR deficiency, showing synergistic lethality when combined with a PARP inhibitor. This study provides a potential new approach for treating TNBC.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2022)
Review
Medicine, Research & Experimental
Michael R. Waarts, Aaron J. Stonestrom, Young C. Park, Ross L. Levine
Summary: Targeted therapies have become increasingly important in cancer treatment, with technological advances improving our understanding of genetic mutations in tumors. In some cases, targeted therapy has dramatically improved treatment outcomes and disease prognoses.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Genetics & Heredity
J. Keller McKowen, Satya V. S. P. Avva, Mukesh Maharjan, Fabiana M. Duarte, Jacob M. Tome, Julius Judd, Jamie L. Wood, Sunday Negedu, Yunkai Dong, John T. Lis, Craig M. Hart
Summary: The study reveals that BEAF physically interacts with the Pbro subunit of PBAP, which plays a subtle role in gene activation and chromatin structure. The interaction between BEAF and Pbro is more important in regulated genes, while other redundant factors may mask their roles in BEAF-associated promoters.
G3-GENES GENOMES GENETICS
(2022)
Article
Plant Sciences
Soujanya Akella, Xinrong Ma, Romana Bacova, Zachary P. Harmer, Martina Kolackova, Xiaoxue Wen, David A. Wright, Martin H. Spalding, Donald P. Weeks, Heriberto Cerutti
Summary: Programmable site-specific nucleases like CRISPR/Cas9 have enabled precise gene editing in Chlamydomonas reinhardtii. A new strategy using pairs of CRISPR/Cas9 RNPs and ssODNs without exogenous dsDNA has shown promise in recovering precise edits in target genes lacking a selectable phenotype, by selecting for edits in another gene that confers a selectable marker. This approach could potentially allow for the creation of individual strains with mutations in multiple target genes for studying complex cellular processes.
Article
Biotechnology & Applied Microbiology
Masaki Endo, Satoshi Iwakami, Seiichi Toki
Summary: Genome editing using artificial nucleases like CRISPR/Cas9 is popular in plants, but inducing nucleotide substitutions is challenging. To address this, a base editor utilizing a fusion of Cas9 and deaminase has been developed, but practical substitution is still limited. In higher plants, the rare homologous recombination (HR)-mediated gene targeting (GT) is achieved using positive-negative selection and a seamless piggyBac transposon-mediated marker elimination system can efficiently eliminate the marker.
PLANT BIOTECHNOLOGY JOURNAL
(2021)
Article
Oncology
Genevieve Dall, Cassandra J. J. Vandenberg, Ksenija Nesic, Gayanie Ratnayake, Wenying Zhu, Joseph H. A. Vissers, Justin Bedo, Jocelyn Penington, Matthew J. J. Wakefield, Damien Kee, Amandine Carmagnac, Ratana Lim, Kristy Shield-Artin, Briony Milesi, Amanda Lobley, Elizabeth L. L. Kyran, Emily O'Grady, Joshua Tram, Warren Zhou, Devindee Nugawela, Kym Pham Stewart, Reece Caldwell, Lia Papadopoulos, Ashley P. P. Ng, Alexander Dobrovic, Stephen B. B. Fox, Orla McNally, Jeremy D. D. Power, Tarek Meniawy, Teng Han Tan, Ian M. M. Collins, Oliver Klein, Stephen Barnett, Inger Olesen, Anne Hamilton, Oliver Hofmann, Sean Grimmond, Anthony T. T. Papenfuss, Clare L. L. Scott, Holly E. E. Barker
Summary: This study identified mutations in DNA repair genes in a subset of uterine leiomyosarcoma patients and found that these patients may benefit from PARP inhibitor therapy. Patient-derived xenograft models confirmed the efficacy of this treatment approach.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2023)
Article
Multidisciplinary Sciences
Sota Shinkado, Hiroki Saito, Masaya Yamazaki, Shunsuke Kotera, Takayuki Arazoe, Tsutomu Arie, Takashi Kamakura
Summary: Researchers have developed a genome editing technology using the CRISPR/Cas9 system for editing Fusarium species, demonstrating efficient and targeted gene knock-out and editing capabilities. The system can be applied to a broad range of Fusarium species for different research purposes.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Xu Zhao, Yuchen Sun, Xuanzi Sun, Jing Li, Xiaobo Shi, Zhinan Liang, Yuan Ma, Xiaozhi Zhang
Summary: This study demonstrated that AEG-1 depletion enhances radiosensitivity and promotes apoptosis as well as cell cycle arrest in G2 phase of glioma cells. Moreover, RFC5 was identified as the target of AEG-1 and associated with poor prognosis of glioma patients. These findings suggest that AEG-1 may serve as a reliable radiosensitizing target for glioma radiotherapy.
DNA AND CELL BIOLOGY
(2021)
Article
Cell Biology
Shang-Hung Chen, Wen-Tsung Huang, Wan-Chen Kao, Sheng-Yen Hsiao, Hsin-Yi Pan, Chin-Wen Fang, Yow-Ling Shiue, Chia-Lin Chou, Chien-Feng Li
Summary: The study demonstrated that MGMT plays a crucial role in the activation of the HR pathway and regulates DDR in NPC cells treated with CDDP and PARP inhibitor. Inhibition of MGMT can increase DSB formation induced by CDDP, decrease HR activity, and enhance cell death and DSB formation caused by PARP inhibitor in NPC cells.
JOURNAL OF BIOMEDICAL SCIENCE
(2021)
Article
Multidisciplinary Sciences
S. V. Satya Prakash Avva, Craig M. Hart
Article
Developmental Biology
Craig M. Hart
DEVELOPMENTAL BIOLOGY
(2014)
Article
Biochemistry & Molecular Biology
Nan Jiang, Eldon Emberly, Olivier Cuvier, Craig M. Hart
MOLECULAR AND CELLULAR BIOLOGY
(2009)
Article
Biochemistry & Molecular Biology
Swarnava Roy, Nan Jiang, Craig M. Hart
MOLECULAR GENETICS AND GENOMICS
(2011)
Article
Biochemistry & Molecular Biology
Alexey A. Soshnev, Bing He, Ryan M. Baxley, Nan Jiang, Craig M. Hart, Kai Tan, Pamela K. Geyer
NUCLEIC ACIDS RESEARCH
(2012)
Article
Multidisciplinary Sciences
Shraddha Shrestha, Dong-Ha Oh, J. Keller McKowen, Maheshi Dassanayake, Craig M. Hart
Article
Multidisciplinary Sciences
Mukesh Maharjan, Robert K. Maeda, Francois Karch, Craig M. Hart
Article
Biochemistry & Molecular Biology
Eldon Emberly, Roxane Blattes, Bernd Schuettengruber, Magali Hennion, Nan Jiang, Craig M. Hart, Emmanuel Kas, Olivier Cuvier
Article
Genetics & Heredity
Yuankai Dong, S. V. Satya Prakash Avva, Mukesh Maharjan, Janice Jacobi, Craig M. Hart
Article
Genetics & Heredity
Mukesh Maharjan, J. Keller McKowen, Craig M. Hart
Article
Genetics & Heredity
J. Keller McKowen, Satya V. S. P. Avva, Mukesh Maharjan, Fabiana M. Duarte, Jacob M. Tome, Julius Judd, Jamie L. Wood, Sunday Negedu, Yunkai Dong, John T. Lis, Craig M. Hart
Summary: The study reveals that BEAF physically interacts with the Pbro subunit of PBAP, which plays a subtle role in gene activation and chromatin structure. The interaction between BEAF and Pbro is more important in regulated genes, while other redundant factors may mask their roles in BEAF-associated promoters.
G3-GENES GENOMES GENETICS
(2022)
Article
Genetics & Heredity
Swarnava Roy, Matthew K. Gilbert, Craig M. Hart
Article
Biochemistry & Molecular Biology
Swarnava Roy, Yian Yee Tan, Craig M. Hart
MOLECULAR GENETICS AND GENOMICS
(2007)
Article
Genetics & Heredity
Matthew K. Gilbert, Yian Yee Tan, Craig M. Hart