Article
Biochemistry & Molecular Biology
Yan Wang, Ting Yang, Hainan Zeng, Junfen Wan, Xuejun Cao
Summary: In this study, a thermo-pH responsive recyclable ATPS was developed for the partitioning of lincomycin, showing good partitioning efficiency and high recoveries of the polymers. This recyclable ATPS has promising industrial applications in the separation and purification of antibiotics.
PROCESS BIOCHEMISTRY
(2021)
Article
Engineering, Chemical
Hao Li, Liangdong Zhao, Shuo Yin, Chuanyu Chen, Haixiong Liu, Jing Fang
Summary: This study highlights the importance of a salt-free extraction system, with the K4P2O7/isopropanol aqueous phase system identified as the optimal choice for biofuel recovery.
Article
Chemistry, Physical
Shaoping Ma, Fenfang Li, Zhijian Tan
Summary: A completely recyclable aqueous two-phase system (ATPS), based on two temperature-responsive polymers, was developed for the chiral separation of mandelic acid enantiomers. The two polymers can be easily recovered and one of them can be used as a chiral selector. High chiral separation efficiency was achieved under specific conditions. This recyclable ATPS provides new insights for other enantioselective liquid-liquid extraction (ELLE) applications.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Article
Chemistry, Physical
Jaran Patchimpet, Kanokphorn Sangkharak, Apiluck Eiad-ua, Sappasith Klomklao
Summary: The study investigated the recovery and partitioning of lipase from Nile tilapia viscera using a T-ATPS system, achieving high yield and partial purification of lipase by optimizing different partitioning parameters and conditions. Ultrasound was found to enhance the activity of lipase, demonstrating its potential as a technology for lipase recovery.
JOURNAL OF MOLECULAR LIQUIDS
(2021)
Article
Biochemistry & Molecular Biology
Phei Er Kee, John Chi-Wei Lan, Hip Seng Yim, Yin Hui Chow, Po-Ting Chen, Hui-Suan Ng
Summary: Keratinase is efficiently recovered using aqueous two-phase electrophoresis system, achieving high partition coefficient, recovery yield, and purification fold under optimal conditions. The introduction of an electric field in the system significantly enhances the selectivity towards extracellular keratinase, demonstrating the efficiency of ATPES in separating keratinase from crude feedstock.
PROCESS BIOCHEMISTRY
(2021)
Article
Polymer Science
Alexander Plucinski, Bernhard V. K. J. Schmidt
Summary: ATPS and water-in-water emulsions are important topics in chemistry and biology due to their biocompatibility and various applications. This study investigates ATPS formed from PDMA with different molar masses and commercial pullulan. It also transforms the ATPS into a pH-responsive water-in-water emulsion, which can be destabilised or stabilised depending on the pH, opening up new pathways for biomolecule encapsulation.
Article
Engineering, Environmental
Yinuo Li, Tingting Wei, Long Chen, Kaixiang Wang, Yulin Shi
Summary: In this study, a salt/water system is developed for efficient separation of zwitterionic polymer and dye from chemical sludge. It is found that at high salt concentration, the separation factor greatly increases and the regenerated polymer fluids exhibit good reusability.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Review
Food Science & Technology
Jun Xi, Xinbiao Zhou, Yu Wang, Shuang Wei
Summary: This review summarizes the research progress of short-chain alcohol/salt-based aqueous two-phase systems (ATPS) for the recovery of plant active compounds. It includes the principle and mechanism, the main factors affecting the efficiency, recent applications, and future perspectives. The findings show that alcohol/salt-based ATPS can significantly improve the purity of extracts and achieve higher extraction efficiency with the aid of ultrasound or microwave. Therefore, alcohol/salt-based ATPS plays a crucial role in improving the recovery of plant active ingredients and has broader application prospects in industrial production.
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2023)
Article
Chemistry, Physical
Yanrong Cheng, Wenlin Xu, Yaqiong Wang, Xia Guo
Summary: In this study, a water-soluble calixarene, p-sulfonatocalix[4]arene (SC[4]), was used as an adjuvant to improve the performance of the PEG 600/(NH4)(2)SO4 ATPS through multiple interactions. It was found that the formation of the ATPS was enhanced and the transfer of the target molecules into the polymer phase was promoted when SC[4] and SC[4]/imidazole ionic liquid ([C(n)mim]Br) complex were used as the adjuvants.
Article
Biotechnology & Applied Microbiology
Hui Suan Ng, Phei Er Kee, Hip Seng Yim, Joo Shun Tan, Yin Hui Chow, John Chi-Wei Lan
Summary: By investigating the partition behavior of GA in alcohol/salt ATPS, the optimal conditions were determined for the extraction of GA from various natural sources. This separation method can improve yield and reduce costs.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2021)
Article
Food Science & Technology
Xiao-Hua Zhang, Jing-Jing Zheng, Xiang-Dong Qing, Fang Lin, Yan-Ting Yuan, Kai -Long Yang, Jing-Zhe Zhang, Hui -Wen Gu
Summary: The phenolic compounds in Chinese tea were successfully extracted using ATPS and simultaneously analyzed using the ATLD algorithm combined with HPLC-DAD. The proposed method showed potential for distinguishing between different types of Chinese teas.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Engineering, Chemical
Tian Yao, Hongmei Li, Yuanhang Ren, Meilin Feng, Yichen Hu, Huiling Yan, Lianxin Peng
Summary: The report introduces thermo-separating magnetic ionic liquids (MILs) for the first time, detailing the synthesis, characterization, and application of two PPG600-based thermo-separating MILs, which were used to create a novel MILATPS system. The system showed high extraction efficiencies for phenolic compounds, exceeding 90%, and demonstrated potential for industrial treatment of phenolic wastewater with efficient recovery processes.
SEPARATION AND PURIFICATION TECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Damien Degoulange, Raj Pandya, Michael Deschamps, Dhyllan A. Skiba, Betar M. Gallant, Sylvain Gigan, Hilton B. de Aguiar, Alexis Grimaud
Summary: High-resolution Raman imaging is used to investigate the interface of salt-salt aqueous biphasic systems (ABSs) at a molecular level, revealing a gradual change of environment instead of a sharp molecular interface. This knowledge is of great importance in understanding aqueous interfaces and has significant implications in various biological and technological settings, including novel battery technologies.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Engineering, Environmental
Michael J. Sargent, Nigel K. H. Slater, John S. Dennis, Gary J. Smith, Paul S. Pearlman
Summary: This study investigated the separation of Hexamethylenediamine (HMD) from dilute aqueous solutions using phase separation techniques, with sodium hydroxide (NaOH) found to significantly promote the phase separation. It was also discovered that sodium sulfate and sodium carbonate can enhance the phase separation, but are unable to induce phase separation without the presence of sodium hydroxide.
CHEMICAL ENGINEERING JOURNAL
(2021)
Review
Environmental Sciences
Thivaharan Varadavenkatesan, Shraddha Pai, Ramesh Vinayagam, Arivalagan Pugazhendhi, Raja Selvaraj
Summary: Aqueous two-phase system (ATPS) is a technique that is increasingly being recognized for its ability to improve yield and purity in a single step, making it a preferred choice for concentrating, separating, and purifying target products from industrial waste discharges. Novel approaches and optimization techniques are being actively explored to enhance the yield and purity of ATPS, with a growing focus on drawing attention from academia and industries towards developing innovative tweaks to current practices in ATPS.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Biotechnology & Applied Microbiology
Sofia P. M. Silva, Jose A. Teixeira, Celia C. G. Silva
Summary: An enterocin whey solution was incorporated into gelatin/glycerol films to control Listeria monocytogenes. The films were shown to release bacteriocin and preserve anti-listerial activity for up to 90 days. When applied in cheese, the films were able to reduce L. monocytogenes contamination to undetected levels and prevent the migration of the pathogen.
Article
Polymer Science
Eduardo J. Gudina, Marcia R. Couto, Soraia P. Silva, Elisabete Coelho, Manuel A. Coimbra, Jose A. Teixeira, Ligia R. Rodrigues
Summary: A low-cost culture medium (CSLM) was developed for EPS production by Rhizobium viscosum CECT908, using sugarcane molasses and corn steep liquor as the sole ingredients. The EPS produced in this medium showed higher yield stress and flow consistency index, indicating its potential use in Microbial Enhanced Oil Recovery.
Article
Food Science & Technology
Luara Simoes, Natalia Fernandes, Jose Teixeira, Luis Abrunhosa, Disney Ribeiro Dias
Summary: Food and feed contamination by fungi, especially toxigenic ones, is a global concern. Lactic acid bacteria (LAB) have been gaining attention for their antifungal and anti-mycotoxin properties. This study tested 14 LAB strains isolated from naturally fermented Brazilian table olives for their ability to inhibit the growth of certain fungi and reduce mycotoxin production. The results showed that certain LAB strains exhibited strong antifungal activity and reduced the production of mycotoxins such as Aflatoxin B1, ochratoxin A, and patulin. These findings demonstrate the potential of LAB strains as natural agents to control toxigenic fungi and their mycotoxins in fermented products.
Review
Biotechnology & Applied Microbiology
Emanuel Cunha, Vitor Sousa, Pedro Geada, Jose A. Teixeira, Antonio A. Vicente, Oscar Dias
Summary: Microalgae are a sustainable and renewable source for various compounds used in industries such as cosmetics, food, and pharmaceuticals. However, their commercialization faces challenges due to low biomass and productivity. Strategies to enhance cost-effectiveness in microalgae cultivation focus on optimizing nutrient needs and environmental factors. Genome-scale metabolic (GSM) models aid in understanding metabolic processes and can be used to optimize compound and biomass production. This review provides an overview of key considerations and strategies in microalgae cultivation, emphasizing the use of GSM models for in silico-guided optimization.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)
Article
Microbiology
Joao Simoes, Eduardo Coelho, Paulo Magalhaes, Tiago Brandao, Pedro Rodrigues, Jose Antonio Teixeira, Lucilia Domingues
Summary: This study explores the potential of non-Saccharomyces yeasts to produce low-alcohol or non-alcoholic beers. Selected yeasts with low-to-null ethanol production were used in large-scale fermentations, resulting in beers with ethanol concentrations below 1.2% and the presence of fusel alcohols and esters. Lager beers produced with Saccharomyces yeast were more accepted by taster panelists. This research demonstrates the suitability of non-conventional yeasts for producing low-alcohol or non-alcoholic beers and opens perspectives for the development of non-conventional beers.
Article
Biotechnology & Applied Microbiology
Catarina Teixeira-Guedes, Joana S. Gomes-Dias, Sara A. Cunha, Manuela E. Pintado, Ricardo N. Pereira, Jose A. Teixeira, Cristina M. R. Rocha
Summary: The sequential use of enzymes in seaweeds has been shown to significantly improve extraction yield, solubilize carbohydrates and protein, and enhance antioxidant and prebiotic effects.
FOOD AND BIOPRODUCTS PROCESSING
(2023)
Article
Biotechnology & Applied Microbiology
Pedro Geada, Diogo Francisco, Francisco Pereira, Filipe Maciel, Leandro Madureira, Ana Barros, Joana L. Silva, Antonio A. Vicente, Jose A. Teixeira
Summary: Microalgae have gained attention as a promising feedstock for healthier food products, but high production costs and low productivities remain a challenge. This study used a two-step optimization strategy to maximize biomass production of a Chlorella vulgaris strain under heterotrophic conditions. After studying 24 variables, (NH4)2SO4, MgSO4middot7H2O, and C6H12O6 were identified as factors with a significant impact on biomass concentration. Optimization of the culture medium led to a 99.6% increase in biomass and a 48.0% increase in carbohydrate production.
FOOD AND BIOPRODUCTS PROCESSING
(2023)
Article
Chemistry, Applied
Hassan Laaroussi, Pedro Ferreira-Santos, Zlatina Genisheva, Meryem Bakour, Driss Ousaaid, Asmae El Ghouizi, Jose Antonio Teixeira, Badiaa Lyoussi
Summary: Bee pollen from Morocco was studied for its physicochemical, nutritional, biological, and techno-functional properties. The samples showed acceptable quality and had major constituents like carbohydrates, lipids, proteins, and polyphenols. The pollen exhibited good techno-functional properties and had potent antioxidant capacity and digestive enzyme inhibitory activity. The study suggests the potential application of bee pollen as a useful ingredient in the food industry.
Article
Chemistry, Applied
M. C. Coelho, A. S. Rodrigues, J. A. Teixeira, M. E. Pintado
Summary: The tomato processing industry is vital for the global market, aiming to optimize production and minimize waste while ensuring high-quality products. By-products generated in this industry are often discarded as waste, but they contain bioactive compounds with various beneficial properties. Recycling these compounds not only reduces environmental impact but also enables the development of value-added products for different purposes. This review discusses the recovery and utilization of bioactive compounds from tomato by-products within the context of a circular economy.
Review
Biochemistry & Molecular Biology
Francieli Colussi, Hector Rodriguez, Michele Michelin, Jose A. Teixeira
Summary: The economically viable alternative of agricultural by-product bioethanol provides a sustainable solution for expanding the use of renewable energy, ensuring greater energy supply security, and reducing environmental impacts. Improving the efficiency of existing processes is crucial to reduce costs, industrial waste, and carbon footprint. Ionic liquids (ILs) have shown promise in achieving cleaner processes and efficient dissolution and fractionation of lignocellulose for biofuel production. This review analyzes recent advances in biofuel production from lignocellulosic biomass using ILs as pretreatment and highlights cost optimization techniques for robust conversion processes.
Review
Biochemistry & Molecular Biology
Monica Serra, Ana Casas, Duarte Toubarro, Ana Novo Barros, Jose Antonio Teixeira
Summary: Microbial production of hyaluronic acid (HA) is gaining attention due to its increasing demand for industrial applications. HA is a linear, non-sulfated glycosaminoglycan widely distributed in nature, mainly composed of repeating units of N-acetylglucosamine and glucuronic acid. Its unique properties such as viscoelasticity, lubrication, and hydration make it attractive for industries like cosmetics, pharmaceuticals, and medical devices. This review presents and discusses available fermentation strategies for HA production.
Article
Food Science & Technology
Laura Alti-Palacios, Juana Martinez, Jose A. C. Teixeira, Jose S. Camara, Rosa Perestrelo
Summary: This study aimed to investigate the impact of cold pre-fermentative maceration (CPM) treatment on the aromatic and sensory properties of white wines from four grape varieties. A total of 62 aroma compounds were identified using HS-SPME/GC-MS. CPM treatment increased the relative concentration of alcohols, esters, and acids, while the impact on terpenoids varied with grape variety and vintage. Esters made the most significant contribution to the sensory properties, enhancing floral and fruity notes.
Article
Food Science & Technology
Marta C. C. Coelho, Soudabeh Ghalamara, Debora Campos, Tania Braganca Ribeiro, Ricardo Pereira, Antonio S. Rodrigues, Jose A. Teixeira, Manuela Pintado
Summary: Tomato by-products from processing industries in Portugal have the potential to be reused as a source of bioactive compounds. However, there is a lack of reliable national data on these by-products and their physicochemical properties, which hinders effective waste management planning. In this study, selected Portugal companies were recruited to obtain representative samples of by-products, and their physicochemical composition was evaluated. Additionally, an environmentally friendly method called ohmic heating (OH) was used to explore new safe value-added ingredients from these by-products.
Article
Food Science & Technology
Mariana Queiros, Guilherme Pereira, Ana Catarina Leite, Rita Leal, Rui Rodrigues, Jose A. Teixeira, Ricardo N. Pereira
Summary: This study investigates the effects of electrical fields and frequencies on the activity of pectinase in the clarification process of Vinho Verde wine must. The results show that electrical frequency can increase enzymatic activity, especially at lower temperatures. The temperature dependence of the enzyme's activity can be reduced when moderate electrical fields are applied.
FRONTIERS IN SUSTAINABLE FOOD SYSTEMS
(2023)
Article
Biotechnology & Applied Microbiology
Catia Santos-Pereira, Joana Sousa, angela M. A. Costa, Andreia O. Santos, Teresa Rito, Pedro Soares, Ricardo Franco-Duarte, Sara C. Silverio, Ligia R. Rodrigues
Summary: In this study, compost samples from three Portuguese companies were analyzed to identify and characterize carbohydrate-active enzymes (CAZymes) and microbial communities. The results showed that the compost samples were rich in glycoside hydrolases (GH), especially cellulases and oligosaccharide-degrading enzymes. The microbiome of the samples was dominated by bacteria, with the classes Gammaproteobacteria, Alphaproteobacteria, and Balneolia being the most abundant. Additionally, comparison with other samples indicated that composting is an excellent source of lignocellulose-degrading enzymes. This is the first comparative study on the abundance and taxonomic/functional profiles of CAZymes in Portuguese compost samples.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Thermodynamics
Haolei Yang, Xiaoqiang Bian, Lianguo Wang
Summary: A new association model, CPA-MHV1, combining the SRK equation of state with the CPA equation based on Michelsen's improved Huron-Vidal mixing rule, is developed. The model is used to investigate the vapor-liquid equilibrium of binary mixtures involving CH4, CO2, and H2O, as well as ternary mixtures containing NaCl. The results show that considering the solvation between CO2 and H2O yields the best performance, while CH4 with a pseudo-association scheme has the highest comprehensive prediction performance.
FLUID PHASE EQUILIBRIA
(2024)
Article
Thermodynamics
Vikas K. Sinha, Atanu K. Metya, Chandan K. Das
Summary: In this study, the solid-liquid coexistence curve for water was developed using coarse-grained mW and machine-learned ML-BOP water models. The ML-BOP model exhibited lower densities, a broader density-temperature hysteresis loop, and higher free energy compared to the mW model. The pressure dependence fusion curve for both models aligned with previous literature. This analysis demonstrates that the free energy method accurately captures the solid-liquid transformation and the thermodynamic melting point of water.
FLUID PHASE EQUILIBRIA
(2024)
Article
Thermodynamics
Curtis Hays Whitson
Summary: This article tells the story of an extraordinary individual who made numerous and valued contributions, portrayed through a few images and short stories.
FLUID PHASE EQUILIBRIA
(2024)
Article
Thermodynamics
Joe Hajjar, Sabine Enders
Summary: A generalized theoretical framework is introduced for multicomponent reactive nonuniform systems, combining the modified and generalized Cahn-Hilliard equation with a chemical kinetics model. The framework allows for the modelling and investigation of the combined reaction and diffusion in reactive liquid-liquid systems. It is found that for reacting mixtures with much smaller reaction rates than diffusion rates, the mixture will remain in phase equilibrium upon reaction and the interfacial chemical reaction will not affect the overall system dynamics, which are only influenced by the kinetics of the bulk phases.
FLUID PHASE EQUILIBRIA
(2024)
Article
Thermodynamics
Keycianne da Cruz Silva, Leticia Daniela de Souza, Vinicius Azevedo Gomes, Leandro Rodrigues de Lemos
Summary: Phase diagrams of four aqueous two-phase systems were determined experimentally, and conclusions were drawn regarding the effect of temperature on system enthalpy change and the influence of macromolecules on phase separation.
FLUID PHASE EQUILIBRIA
(2024)
Article
Thermodynamics
Yuanqing Wu, Shuyu Sun
Summary: The sparse-grid guided PINN training method improves the accuracy of the PINN surrogate model by rearranging collocation points and reduces the training time complexity. Experimental results demonstrate its clear advantages in terms of convergence, stability, and accuracy.
FLUID PHASE EQUILIBRIA
(2024)
Article
Thermodynamics
Peter Englezos
Summary: This article discusses the research progress and challenges in the field of canonical clathrate or gas hydrate phase equilibria, as well as the application of computational methods and models. It also explores the potential of machine learning techniques in hydrate and thermodynamic calculations.
FLUID PHASE EQUILIBRIA
(2024)
Article
Thermodynamics
Ying-Chieh Hung, Shao-Wei Su, Jia-Wei Yan, Gui-Bing Hong
Summary: In this study, VLE data for five binary systems were measured and successfully correlated using models. The predictive models were used to predict the binary VLE phase diagram and explain the mechanism of separation efficiency. The strength of electrostatic interactions and temperature-dependent separation behavior were determined through the analysis of molecular surface charge distribution.
FLUID PHASE EQUILIBRIA
(2024)
Article
Thermodynamics
Matevz Turk, Tomaz Urbic
Summary: By molecular dynamics and Monte Carlo simulations, this study determined the regions and hierarchy of anomalies in a purely repulsive core-softened system. It was found that in two dimensional systems, a size of 100 particles is sufficient for obtaining accurate details.
FLUID PHASE EQUILIBRIA
(2024)
Article
Thermodynamics
Adnan Jaradat, Rakan Al-Salman, Abdalla Obeidat
Summary: The vapor-liquid equilibrium of six primary alcohols were studied using molecular dynamics simulations and compared with experimental results. The study found that neglecting the Lennard-Jones interaction significantly reduced the critical temperature values and determining the critical temperature using surface tension gave more accurate results. The TraPPE-UA potential field showed good accuracy in predicting the critical temperature.
FLUID PHASE EQUILIBRIA
(2024)
Article
Thermodynamics
Michele Valsecchi, Amparo Galindo, George Jackson
Summary: The thermodynamic properties of aqueous mixtures of polyethylene glycol (PEG) are described using the SAFT-gamma Mie group-contribution equation of state. The model successfully predicts the miscibility gaps in a temperature range and shows good agreement with experimental results.
FLUID PHASE EQUILIBRIA
(2024)
Article
Thermodynamics
Roman Tomaschitz
Summary: Analytic closed-form expressions for the liquid and vapor saturation densities defining the coexistence curve are obtained. The coexistence curves of nitrogen, ethene, and sulfur hexafluoride are derived using high-precision data, and the critical power-law scaling of the order parameter and coexistence-curve diameter is examined. The Log-Log slopes of various quantities are used to determine the ideal power-law scaling regime and illustrate the slope evolution in the subcritical regime.
FLUID PHASE EQUILIBRIA
(2024)
Article
Thermodynamics
Nivaar Brijmohan, Kuveneshan Moodley, Caleb Narasigadu
Summary: An artificial neural network-based QSPR model was developed to estimate binary interaction parameters for the temperature-dependant form of the NRTL model. This model serves as a supplement to overcome limitations of group contribution methods in solvent screening and has been proven to be effective in improving the robustness of the screening process.
FLUID PHASE EQUILIBRIA
(2024)
Article
Thermodynamics
Marco Campestrini, Paolo Stringari, Salem Hoceini
Summary: In this paper, the solubility of benzene in different phases of methane was predicted using the GERG-2008 equation of state and the Gibbs free energy equation. The obtained results were in good agreement with experimental data and emphasized the significance of accurately representing the density of the fluid phase for predicting the solubility of solids in fluids.
FLUID PHASE EQUILIBRIA
(2024)
Article
Thermodynamics
Nefeli Novak, Georgios M. Kontogeorgis, Marcelo Castier, Ioannis G. Economou
Summary: This paper discusses theoretical considerations on electrolyte thermodynamic models for single and mixed solvent solutions. The authors find that there is no consensus among researchers on many fundamental issues in this field. They point out the need to convert electrolyte terms to the framework of the physical model and emphasize the importance of the Born term for liquid-liquid equilibrium calculations.
FLUID PHASE EQUILIBRIA
(2024)
