Article
Biochemistry & Molecular Biology
Libo Zhang, Hai Yu, Yuanyuan Bai, Bijoyananda Mishra, Xiaoxiao Yang, Jing Wang, Evan B. Yu, Riyao Li, Xi Chen
Summary: Carbohydrate-protein conjugates have diverse applications, from vaccines to high-throughput assays. A process combining chemoenzymatic synthesis, squarate linker conjugation, and neoglycoprotein immobilization on fluorescent magnetic beads was developed for a suspension multiplex array platform. This platform allowed for analysis of glycan and GBP interactions, providing useful insights into human galectins' multivalent glycan-binding properties.
Article
Ecology
Vojtech Abraham, Sheila Hicks, Helena Svobodova-Svitavska, Elissaveta Bozilova, Sampson Panajiotidis, Mariana Filipova-Marinova, Christin Eldegard Jensen, Spassimir Tonkov, Irena Agnieszka Pidek, Joanna Swieta-Musznicka, Marcelina Zimny, Eliso Kvavadze, Anna Filbrandt-Czaja, Martina Hattestrand, Nurgul Karlioglu Kilic, Jana Kosenko, Maria Nosova, Elena Severova, Olga Volkova, Margret Hallsdottir, Laimdota Kalnina, Agnieszka M. Noryskiewicz, Bozena Noryskiewicz, Heather Pardoe, Areti Christodoulou, Tiiu Koff, Sonia L. Fontana, Teija Alenius, Elisabeth Isaksson, Heikki Seppa, Siim Veski, Anna Pedziszewska, Martin Weiser, Thomas Giesecke
Summary: The collection of modern pollen data in Europe has shown that forest cover and climate parameters influence pollen accumulation rates, and long-distance transport of pollen grains is possible. Comparisons between modern and fossil pollen data demonstrate similar values, aiding in interpretations of fossil data.
Article
Chemistry, Organic
Alejandro E. Cristofalo, Pedro M. Nieto, Michel Thepaut, Franck Fieschi, Pablo H. Di Chenna, Maria Laura Uhrig
Summary: The synthesis of an octavalent glycocluster exposing a thiodisaccharide mimetic of hyaluronic acid was reported, showing strong self-aggregation. Experimental results confirmed the formation of spherical micelles with a diameter of approximately 6.2 nm and interaction with Langerin, a C-type lectin. This interaction was attributed to a multivalent effect not observed in experiments with basic disaccharides.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Dania Martinez-Alarcon, Viviane Balloy, Jean-Philippe Bouchara, Roland J. Pieters, Annabelle Varrot
Summary: SapL1 is a lectin from S. apiospermum that shows specificity for fucosylated oligosaccharides and binds to bronchial epithelial cells in a fucose-dependent manner, making it a potential drug target.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Physical
Saradamoni Mondal, Kishore Babu Bobbili, Sumanta Paul, Musti J. Swamy
Summary: The study on the thermal and chemical unfolding mechanism of Coccinia indica agglutinin (CIA17) revealed that the protein unfolds in multiple steps, including oligomer dissociation, dimer dissociation, and monomer unfolding. Fluorescence correlation spectroscopic studies showed that CIA17 forms oligomeric structures in a concentration dependent manner, playing a key role in the plant's defense response against microbes and insects.
JOURNAL OF PHYSICAL CHEMISTRY B
(2021)
Article
Chemistry, Organic
Sourav Misra, Sandip Paul, Sourav Pakrashy, Sayan Ghosh, Susmita Naskar, Pawan Kumar Maurya, Pinki Saha Sardar, Katta Venkateswarlu, Adity Bose, Anjoy Majhi
Summary: A novel 1,2,3-triazole-napthamide molecule (SSAM-1) has been designed and synthesized using De-Novo drug design method and copper-catalyzed alkyne-azide cycloaddition reaction. The interaction of SSAM-1 with proteins and enzymes has been studied and validated using fluorescence spectroscopy and molecular docking. ADMET prediction indicates the potential drug-ability of SSAM-1. These studies contribute to the early-stage drug design process.
INDIAN JOURNAL OF CHEMISTRY
(2023)
Article
Plant Sciences
Petr Dvorak, Yuliya Krasylenko, Miroslav Ovecka, Jasim Basheer, Veronika Zapletalova, Jozef Samaj, Tomas Takac
Summary: The study investigates the developmental expression and subcellular localization of FSD1 in Arabidopsis using microscopy techniques, revealing its importance in seed germination and lateral root development. FSD1 plays a crucial role in salt stress tolerance and lateral root formation, with a major subcellular pool located in the plastid stroma.
PLANT CELL AND ENVIRONMENT
(2021)
Review
Biochemistry & Molecular Biology
Na Lian, Xinwei Wang, Yanping Jing, Jinxing Lin
Summary: The plant cytoskeleton undergoes dynamic remodeling in response to developmental and environmental cues, with microtubule-associated proteins and actin-binding proteins playing key roles in modulating cytoskeletal behavior. The activities and levels of MAPs and ABPs are precisely regulated during development and environmental responses, but our understanding of this process remains limited.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Miroslava Hrbackova, Ivan Luptovciak, Katerina Hlavackova, Petr Dvorak, Michaela Ticha, Olga Samajova, Dominik Novak, Hanna Bednarz, Karsten Niehaus, Miroslav Ovecka, Jozef Samaj
Summary: Transgenic manipulation of SIMK in alfalfa plants infected with Sinorhizobium meliloti showed that downregulation of both SIMKK and SIMK led to reduced root hair growth and lower capacity for infection thread and nodule formation, while overexpression of GFP-tagged SIMK promoted root hair growth and clustering of infection threads and nodules. These findings suggest that genetic manipulations affecting SIMK expression have significant impacts on root hair, nodule, and shoot formation patterns in alfalfa, indicating the biotechnological potential of this MAPK.
PLANT BIOTECHNOLOGY JOURNAL
(2021)
Editorial Material
Plant Sciences
Jingjing Xing, Zhikun Duan, Liang Zhang, Xi Zhang, Jinxing Lin
TRENDS IN PLANT SCIENCE
(2021)
Review
Plant Sciences
Jingjing Xing, Liang Zhang, Zhikun Duan, Jinxing Lin
Summary: The article provides a summary of defense-associated signal transduction processes in plants, focusing on the connection between phospholipid-based signaling and membrane trafficking. It discusses the role of lipid diversity in these processes and highlights the mechanisms that regulate plant immunity by mediating feedback between phospholipid-based signaling and membrane trafficking.
TRENDS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Lenka Kubenova, Tomas Takac, Jozef Samaj, Miroslav Ovecka
Summary: The der1-3 mutant carrying a single-point mutation in the ACTIN2 gene shows enhanced plant growth under oxidative stress, with a more dynamic actin cytoskeleton and higher antioxidant activity. These results suggest that alterations in the actin cytoskeleton and increased capacity to decompose reactive oxygen species contribute to the mutant's enhanced tolerance against oxidative stress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Editorial Material
Genetics & Heredity
Yanmei Chen, Chunxiang Fu, Zhenying Wu, Huimin Xu, Hongmei Liu, Harald Schneider, Jinxing Lin
TRENDS IN GENETICS
(2021)
Review
Plant Sciences
Petr Dvorak, Yuliya Krasylenko, Adam Zeiner, Jozef Samaj, Tomas Takac
Summary: Reactive oxygen species (ROS) are essential signaling molecules in plant responses to environmental stimuli and development. Antioxidant enzymes play a critical role in scavenging ROS and their regulation is influenced by both environmental conditions and transcriptional activation.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Bodan Su, Xi Zhang, Li Li, Sammar Abbas, Meng Yu, Yaning Cui, Frantisek Baluska, Inhwan Hwang, Xiaoyi Shan, Jinxing Lin
Summary: This study provides insight into how the precise control of BSK1 spatiotemporal organization regulates signaling specificity to balance plant growth and immunity.
Article
Biochemical Research Methods
Huimin Xu, Yuanyuan Zhao, Yuanzhen Suo, Yayu Guo, Yi Man, Yanping Jing, Xinqiang He, Jinxing Lin
Summary: This study introduces a label-free imaging technology, coherent Raman scattering (CRS) microscopy, for visualizing the major structures and chemical composition of plant cell walls. This rapid approach can help researchers understand the highly heterogeneous structures and organization of plant cell walls.
Article
Plant Sciences
Tomas Takac, Pavel Krenek, George Komis, Pavol Vadovic, Miroslav Ovecka, Ludmila Ohnoutkova, Tibor Pechan, Petr Kasparek, Tereza Ticha, Jasim Basheer, Mark Arick, Jozef Samaj
Summary: This study demonstrates the significant role of HvMPK3 in barley's response to the bacterial elicitor flg22, with knock-out lines showing downregulation of defense-related proteins and reduced susceptibility to flg22. The differential proteomic analysis and phenotypic observations suggest a specific molecular and root hair response of barley to flg22 in HvMPK3 KO lines.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Yuliya Krasylenko, George Komis, Sofiia Hlynska, Tereza Vavrdova, Miroslav Ovecka, Tomas Pospisil, Jozef Samaj
Summary: Strigolactones regulate developmental events in roots by affecting the organization and dynamics of cortical microtubules. Chemical and genetic manipulation of strigolactone signaling impacts microtubule remodeling, especially under light conditions. The application of strigolactones in dark conditions partially alleviates cytoskeletal rearrangement, suggesting a new mechanistic connection between cytoskeletal behavior and light-dependent strigolactone signaling.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Despina Samakovli, Tereza Ticha, Tereza Vavrdova, Natalie Zavorkova, Ales Pecinka, Miroslav Ovecka, Jozef Samaj
Summary: The YODA kinase pathway is closely related to Arabidopsis embryo development control, with HSP90 proteins emerging as key regulators. Genetic interactions between HSP90 and YDA affect downstream signaling pathways, impacting basal and apical cell lineage development. Spatial-temporal expression of specific genes is altered when HSP90 or YDA function is impaired, suggesting a crucial role in cell fate determination and potential connection to auxin signaling during early embryo development.
Article
Plant Sciences
Laining Zhang, Tetyana Smertenko, Deirdre Fahy, Nuria Koteyeva, Natalia Moroz, Anna Kucharova, Dominik Novak, Eduard Manoilov, Petro Smertenko, Charitha Galva, Jozef Samaj, Alla S. Kostyukova, John C. Sedbrook, Andrei Smertenko
Summary: The study focuses on the function of formins in cytokinesis, suggesting their roles in promoting microtubule polymerization, nucleating F-actin at the cell plate, retaining dynamin-related proteins at the cell plate, and remodeling the cell plate membrane. The use of SMIFH2 has helped reveal the impact of formins on these processes.
Article
Biochemistry & Molecular Biology
Despina Samakovli, Tereza Ticha, Jozef Samaj
PLANT SIGNALING & BEHAVIOR
(2020)