期刊
FITOTERAPIA
卷 90, 期 -, 页码 199-208出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.fitote.2013.07.020
关键词
Mangiferin; Isomangiferin; Iriflophenone 3-C-beta-glucoside; Semi-preparative HPLC; Fibroblast-like synovial cells; Rheumatoid arthritis
资金
- Polish Ministry of Education and Science [N N302 041936]
A fast and efficient method for the isolation of the C-glucosidated xanthones mangiferin and isomangiferin from the South-African plant Cyclopia genistoides was developed for the first time. The procedure involved extraction, liquid-liquid partitioning with ethyl acetate and subsequent precipitation of mangiferin and isomangiferin from methanol and acetonitrile-water fractions, respectively. Additionally, two benzophenone derivatives: 3-C-beta-glucosides of maclurin and iriflophenone, were isolated from C. genistoides extracts using semi-preparative HPLC. Apart from the above, the isolation procedure also yielded hesperidin and small amounts of luteolin. The structures of the compounds were determined by 1D and 2D NMR experiments and/or LC-DAD-ESI-MS. The selected Cyclopia constituents were screened for pro-apoptotic activity on TNF-alpha-stimulated synovial cells isolated from rheumatoid arthritis patients. The strongest effect, measured as percent of apoptotic cells, was recorded for isomangiferin (75%), followed by iriflophenone 3-C-beta-glucoside (71%), hesperidin (67%) and mangiferin (65%). The results are encouraging for further studies on the use of the above compounds in the treatment of rheumatoid arthritis. (C) 2013 Elsevier B.V. All rights reserved.
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