期刊
FISHERIES SCIENCE
卷 78, 期 6, 页码 1321-1329出版社
SPRINGER JAPAN KK
DOI: 10.1007/s12562-012-0552-y
关键词
Diabetes mellitus; Diabetic complication; Fucoxanthin; PTP1B; Molecular docking; Enzyme kinetic
类别
资金
- Ministry of Food, Agriculture, Forestry and Fisheries in the Republic of Korea [20111001212-00]
- Ministry for Food, Agriculture, Forestry & Fisheries (MAFRA), Republic of Korea [CD20110521] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
In the present study, we investigated the antidiabetic potential of fucoxanthin via the inhibition of rat lens aldose reductase (RLAR), human recombinant aldose reductase (HRAR), advanced glycation end-product (AGE) formation, protein tyrosine phosphatase 1B (PTP1B), and alpha-glucosidase. Fucoxanthin displayed potent inhibitory activity against AGE formation and HRAR and RLAR activity. In addition, fucoxanthin showed potent inhibitory activity against PTP1B. However, it did not show alpha-glucosidase inhibitory activity below 200 mu M. In addition, our kinetic study revealed that fucoxanthin competitively inhibited RLAR, while it showed mixed-type inhibition against PTP1B. In order to confirm enzyme inhibition, we predicted the 3D structure of PTP1B using Autodock 4.0 to simulate the binding of fucoxanthin. Docking simulation results demonstrated that three residues of PTP1B (Phe30, Phe52, and Gly183) interact with the two hydroxyl groups of fucoxanthin. In addition, the binding energy was negative (-7.66 kcal/mol), indicating that the three hydrogen bonds may stabilize the open form of the enzyme and potentiate tighter binding to the active site of PTP1B, resulting in more effective PTP1B inhibition. The results of the present study therefore clearly demonstrate the promising potential of fucoxanthin as a therapeutic intervention for the management of diabetes as well as diabetes-associated complications, which could be explored further.
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