Article
Chemistry, Multidisciplinary
Jing Tu, Yi Qiao, Yuhan Luo, Naiyun Long, Zuhong Lu
Summary: DNA quantification is crucial in life sciences research, and real-time MDA technology can provide template amount information during whole-genome amplification with a very low detection limit, offering a promising tool to quantify entire DNA without sacrificing sample completeness or introducing redundant steps.
Article
Chemistry, Analytical
Liang Gong, Siyue Yao, Yidong He, Chengliang Liu
Summary: A new qPCR data analysis method called AERKM is proposed to achieve more accurate results by introducing amplification efficiency (AE) for error correction. It has been verified to have higher precision and stability in 5-point 10-fold gradient qPCR tests of 63 genes.
ANALYTICAL CHEMISTRY
(2023)
Article
Plant Sciences
Rohit Bharati, Madhab Kumar Sen, Ram Kumar, Aayushi Gupta, Jana Ziarovska, Eloy Fernandez-Cusimamani, Olga Leuner
Summary: This study assessed the suitability of ten commonly used reference genes as optimal reference genes in M. officinalis under different abiotic stress conditions and plant organs. The combination of EF-1 alpha and GAPDH was recommended as the best reference genes for gene expression studies in M. officinalis.
Article
Biochemistry & Molecular Biology
Kathannan Sankar, Hyung Joo Yoon, Young Bo Lee, Kyeong Yong Lee
Summary: Bumble bees are important alternative pollinators and model insects. This study identified stable reference genes for the bumble bee species and their different lines and tissues using RT-qPCR procedures and four analysis programs. The results emphasized the need to evaluate the stability of candidate reference genes for any differently designed lines and tissue conditions in bumble bee species.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agronomy
Shengyi Bai, Xiaomin Wang, Meng Guo, Guoxin Cheng, Abid Khan, Wenkong Yao, Yanming Gao, Jianshe Li
Summary: This study screened out the most stable reference genes and studied the expression of related genes in the interaction between tomatoes and powdery mildew, providing a calibration basis for the expression of related functional genes.
Article
Chemistry, Applied
Tingting Li, Jishi Wang, Zhiying Wang, Lu Qiao, Rui Liu, Shanshan Li, Ailiang Chen
Summary: Mitochondrial genes are commonly used for PCR-based meat adulteration authentication, but variations in gene copies can lead to quantification errors. This study developed a quantitative method using single-copy nuclear genes to accurately identify mutton adulteration, achieving high recovery rates for simulated mutton-pork, mutton-chicken, and mutton-duck samples.
Article
Biochemistry & Molecular Biology
Mengdi Chen, Zhengbo Wang, Ziyuan Hao, Hongying Li, Qi Feng, Xue Yang, Xiaojiao Han, Xiping Zhao
Summary: This study selected suitable internal reference genes for studying gene expression in Broussonetia papyrifera under abiotic stresses. Through RT-qPCR and stability analysis, the best internal reference genes and their combinations were determined for different stress conditions and tissues. The reliability of the screened internal reference genes was further verified by analyzing the expression patterns of target genes. This study lays the foundation for functional analysis and regulatory mechanism research of genes in B. papyrifera.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Physiology
Guo Ding, Qionghua Gao, Jun Chen, Jie Zhao, Guojie Zhang, Weiwei Liu
Summary: This study validates reliable reference genes for RT-qPCR analysis in pharaoh ants and lays the foundation for future research.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Multidisciplinary Sciences
Saqib Rahman, Zhenzhen Zhao, Muhammad Umair Sial, Yanning Zhang, Hongyun Jiang
Summary: This study confirmed the stability of Actin and 18S as reference genes for normalizing the expression of target genes in Myzus persicae under different conditions. Both genes were effective in normalizing the expression levels of target genes under insecticide treatment and starvation. However, Actin was more stable than 18S at the developmental stages of M. persicae.
Article
Biochemical Research Methods
Daniele Tavernelli, Tiziana Calamoneri, Paola Vocca
Summary: Tree reconciliation is a framework for studying the mutual influence between gene and species trees. In this framework, costs are assigned to evolutionary events and the goal is to find a reconciliation with minimum total cost. This study introduces constraints on horizontal gene transfers and proves that the problem remains linear in the dimension of the trees.
IEEE-ACM TRANSACTIONS ON COMPUTATIONAL BIOLOGY AND BIOINFORMATICS
(2022)
Article
Fisheries
Shuangyan Zhang, Xiaoshang Ru, Fang Su, Wenke Liang, Libin Zhang, Hongsheng Yang
Summary: This study selected ten reference genes (RGs) for sea cucumber gonadal development and identified the most stable genes using quantitative real-time PCR analysis. The results showed that EF1 beta and Grb2 were the most stable combination for all gonad samples, while UBQ and RPS4 were the most stable combination for the testes. These findings provide valuable information for further studies on the molecular mechanisms of sea cucumber gonadal development.
AQUACULTURE REPORTS
(2022)
Article
Chemistry, Multidisciplinary
Bong Kyun Kim, Sang-A Lee, Minju Park, Eui Ju Jeon, Mi Jung Kim, Jung Min Kim, Heesuk Kim, Seungwon Jung, Sang Kyung Kim
Summary: This article introduces a photonic PCR platform using hydrogel microparticles, which utilizes photothermal nanomaterials as heating elements for rapid and portable POCT. The researchers successfully demonstrate the ability to perform multiplex assays by loading multiple encoded pPIN microparticles in a single reaction.
Article
Physiology
Jinrong Tang, Gemei Liang, Shaoqi Dong, Shuang Shan, Man Zhao, Xianru Guo
Summary: By evaluating the stability of reference genes in Athetis dissimilis under different conditions, suitable reference genes for different experimental conditions can be determined. Different reference genes are needed under different experimental conditions, providing a reliable basis for studying gene expression in A. dissimilis.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Zhiwei Chen, Nigel G. Halford, Chenghong Liu
Summary: Real-time quantitative PCR is a technique for measuring the content of a specific nucleic acid sequence in a sample, and it can be divided into absolute and relative quantitative methods. Relative quantification is mainly used for gene expression in functional genomics and transcriptome studies. However, to accurately use this technology, key points such as designing specific primers, selecting appropriate reference genes, and performing scientific calculations and statistics are crucial. This article proposes a workflow for relative quantitative PCR and introduces relevant points to help beginners better understand and utilize this technology.
Article
Multidisciplinary Sciences
Hao Zhan, Hanzhang Liu, Tianchong Wang, Lin Liu, Wanfeng Ai, Xiujun Lu
Summary: This study selected 11 candidate reference genes and evaluated their expression stability in different tissues and under various stress conditions in Quercus mongolica. The results showed that the most stable genes varied in different experimental environments. Moreover, the study demonstrated the reliability of the selected reference genes in different tissues and under different stress conditions.
Article
Chemistry, Physical
Sayyedeh Shahrbanoo Zahedi, Arash Larki, Seyyed Jafar Saghanezhad, Yadollah Nikpour
Summary: This research evaluated the potential application of mesoporous SBA-15@DABCO for the selective separation/concentration of Cr(VI) ions in water samples. The method showed a linear calibration curve for Cr(VI) determination with a low limit of detection. It was successfully applied for the determination of Cr(VI) in various environmental water and wastewater samples.
Article
Food Science & Technology
Seyyedeh Zeynab Mosavinia, Seyed Mohammad Mousavi, Ainaz Khodanazary, Seyed Mehdi Hosseini
Summary: The combination of ZnONPs and ZEO with CH coating showed antimicrobial effects and improved the biochemical and color properties of Asian sea bass fillets during refrigeration storage. This combination can optimize the storage time of refrigerated seafood.
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
(2022)
Article
Biochemistry & Molecular Biology
Maryam Mirzaei VandKhanghah, Aliakbar Hedayati, Samaneh Nazeri, Hamid Mohammadi Azarm, Rasoul Ghorbani
Summary: This study investigated the transmission of copper sulfate in the food chain of aquatic organisms, revealing that copper causes histopathological changes in liver tissues and has a strong positive correlation with blood glucose and enzyme levels.
BIOLOGICAL TRACE ELEMENT RESEARCH
(2022)
Article
Chemistry, Analytical
Fahimeh Shajarat, Kamal Ghanemi, Mohammad Alimoradi, Majid Ramezani
Summary: This study successfully synthesised a polydopamine-functionalised biosilica for efficient adsorption of heavy metal ions in different types of water matrices. The method showed good extraction and determination performance, and demonstrated comparable capability in industrial and environmental water samples.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY
(2023)
Article
Environmental Sciences
A. Lotfeali Ayeneh, H. Amanipoor, S. Battaleb-Looie, K. Bagheri Nia
Summary: The study identified and localized geological hazards in the construction phase of Ahvaz Subway Line 1 and assessed the environmental impacts in the surface and subsurface sections. The results showed potential environmental hazards at Stations 1, 2, 11, and 12 in the surface section, and the possibility of subsidence in the subway route due to the type of alluvial material and lithology. Seismic stresses and soil corrosion potential were also identified as key factors to consider along the subway route.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL SCIENCE AND TECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Zeinab Tamoradi Babaei, Arash Larki, Kamal Ghanemi
Summary: In this study, two-dimensional molybdenum disulfide nanosheets were synthesized and utilized as an effective adsorbent for preconcentration of heavy metal ions. Various analytical parameters were investigated to optimize the recovery of target ions, resulting in satisfactory recoveries and low detection limits. The kinetics and adsorption isotherms studies showed good fitting with the experimental data, indicating the potential application of the suggested procedure in environmental water sample analysis.
JOURNAL OF THE IRANIAN CHEMICAL SOCIETY
(2022)
Article
Marine & Freshwater Biology
Sajjad Abdolvand, Sahar Kaedinejadesfahani
Summary: The study investigated the accumulation of heavy metals in contaminated sediments and Sole fish from Musa estuary. Results showed significant differences in metal values between the liver and gill of fish species, with higher metal concentrations found in the liver. Pearson correlation analyses revealed a positive correlation between sediment and fish for metal concentrations. Geoaccumulation index and enrichment factor analyses indicated high pollution with anthropogenic sources in multiple sampling sites.
Article
Environmental Sciences
Parastoo Pourashouri, Hojat Mirsadeghi, Aynaz Khodanazary
Summary: Carotenoprotein extracted from Pacific white shrimp processing waste using alcalase and pepsin showed high protein and carotenoid content, with essential amino acids and dominant non-essential amino acids. The extracted samples also contained high amounts of mono and polyunsaturated fatty acids, with differences in fatty acid compositions between alcalase and pepsin extracted samples. Mineral content was significant in the extracted samples, with higher levels of certain minerals in alcalase-extracted samples. The results suggest that carotenoprotein from processing residue of Pacific white shrimp can be used as a valuable and nutritious food or feed powder.
WASTE AND BIOMASS VALORIZATION
(2022)
Article
Pharmacology & Pharmacy
Abdolazim Behfar, Narges Aqajari, Mohammad Reza Shushizadeh, Zahra Ramezani, Ebrahim Rajabzadeh Ghatrami
Summary: This study aimed to extract, purify, and characterize alginate from Sargassum angustifolium simultaneous with fucoidan extraction, and to investigate the effect of this process on the structure and properties of alginate. The obtained results showed the removal of 66.54% impurities, such as phenolic compounds, from the alginate extract. The M/G ratio, intrinsic viscosity, and molecular weight of alginate were determined to be 0.61, 0.9 dL/g, and 41.53 kDa, respectively.
JUNDISHAPUR JOURNAL OF NATURAL PHARMACEUTICAL PRODUCTS
(2022)
Article
Fisheries
Mohammad Hossein Khanjani, Gholamreza Ghaedi, Moslem Sharifinia
Summary: The study demonstrated that adding 0.2% beta-glucan to the diet of rainbow trout significantly improves growth performance, survival, and various haematological, immune, and biochemical parameters.
AQUACULTURE RESEARCH
(2022)
Article
Environmental Sciences
Forough Tajiki, Hossein Mohammad Asgari, Isaac Zamani, Farshid Ghanbari
Summary: This study assessed the impact of two dust sources on the concentration and diversity of airborne fungi, as well as the contribution of dust sources to airborne fungi. The results showed that dust from Hor al-Azim and Shadegan contributed significantly to the ambient fungal community in the study area.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Engineering, Marine
Adel Kassari, Mehdi Behdarvandi Askar
Summary: Submarine pipelines are a safe way to transport petroleum products, and studying the technical knowledge related to them is crucial. The experiments showed that factors such as pipeline diameter, depth, and burial depth influence the force applied to the submarine pipelines, with the absorption method providing reliable results.
SHIPS AND OFFSHORE STRUCTURES
(2022)
Article
Marine & Freshwater Biology
Khadijeh Khalifi, Negin Salamat, Abdolali Movahedinia
Summary: This study investigated the distribution and activity of Na+/K+-ATPase pump in the posterior gills of three species of crabs from different habitats. The results showed that the activity of NKA pump was higher in estuarial and marine species, while freshwater species had lower dependence on this enzyme.
OCEAN SCIENCE JOURNAL
(2022)
Article
Fisheries
Mahbubeh Yusefi, Hamid Mohammadiazarm, Amir Parviz Salati
Summary: The study suggests that NaDF can be a good alternative to antibiotics for improving fish performance, with 5 g/Kg NaDF significantly improving growth performance, immune parameters, and survival rate after thermal stress in juvenile common carp.
AQUACULTURE REPORTS
(2022)
Article
Chemistry, Analytical
Fahimeh Shajarat, Kamal Ghanemi, Mohammad Alimoradi, Majid Ramezani
Summary: This study successfully synthesized a nanostructured composite of diatomite-based biosilica/polydopamine for extraction and preconcentration of five important phthalic acid esters from aqueous samples. Through optimization of key parameters, effective analysis and detection of the samples were achieved, resulting in favorable outcomes.
MICROCHEMICAL JOURNAL
(2022)