期刊
FISH & SHELLFISH IMMUNOLOGY
卷 36, 期 1, 页码 52-60出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2013.10.010
关键词
Sulfated galactans; Gracilaria fisheri; Immune stimulator; White spot syndrome virus (WSSV)
资金
- Thailand Research Fund (TRF) [RSA 5580037]
- Thailand Research Fund (TRF MAG) [MRG-WI535S074]
- Faculty of Science, Mahidol University
Sulfated galactans (SG) were isolated from the red seaweed Gracilaria fished (G. fisher. Chemical analysis revealed SG contains sulfate (12.7%) and total carbohydrate (42.2%) with an estimated molecular mass of 100 kDa. Structure analysis by NMR and FT-IR spectroscopy revealed that SG is a complex structure with a linear backbone of alternating 3-linked beta-D-galactopyranose and 4-linked 3,6-anhydrogalactose units with partial 6-O-methylate-beta-D-galactopyranose and with sulfation occurring on C4 of D-galactopyranose and C6 of L-galactopyranose units. SG treatment enhanced immune parameters including total haemocytes, phenoloxidase activity, superoxide anions and superoxide dismutase in shrimp Penaeus monodon. Shrimp fed with Anemia sauna enriched with SG (100 and 200 mu g ml(-1)) and inoculated with white spot syndrome virus (WSSV) showed a significantly lower mortality rate and lower viral VP 28 amplification and expression than control. The results suggest that SG from G. fisheri exhibits immune stimulatory and antiviral activities that could protect P. monodon from WSSV infection. (C) 2013 Elsevier Ltd. All rights reserved.
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